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您的检索式：作者名="WEI Minxin"
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| 1 | Commutative regulation between endothelial NO synthase and insulin receptor substrate 2 by microRNAs显示文摘Endothelial NO synthase (eNOS) expression is regulated by a number of transcriptional and post-transcriptional mechanisms, but the effects of competing endogenous RNAs (ceRNAs) on eNOS mRNA and the underlying mechanisms are still unknown. Our bioinformatic analysis revealed three highly expressed eNOS-targeting miRNAs (miR-15b, miR-16, and miR-30b) in human endothelial cells (ECs). Among the 1103 mRNA targets of these three miRNAs, 15 mRNAs share a common disease association with eNOS. Gene expression and correlation analysis in patients with cardiovascular diseases identified insulin receptor substrate 2 (IRS2) as the most correlated eNOS-ceRNA. The expression levels of eNOS and IRS2 were coincidentally increased by application of laminar shear but reduced with eNOS or IRS2 siRNA transfection in human ECs, which was impeded by Dicer siRNA treatment. Moreover, luciferase reporter assay showed that these three miRNAs directly target the 3′UTR of eNOS and IRS2. Overexpression of these three miRNAs decreased, whereas inhibition of them increased, both mRNA and protein levels of eNOS and IRS2. Functionally, silencing eNOS suppressed the Akt signal pathway, while IRS2 knockdown reduced NO production in ECs. Thus, we identified eNOS and IRS2 as ceRNAs and revealed a novel mechanism explaining the coincidence of metabolic and cardiovascular diseases. | Xiaoli Sun Huizhen Lv Peng Zhao Jinlong He Qinghua Cui Minxin Wei Shiqing Feng Yi Zhu | 2019 | Journal of Molecular Cell Biology2019，11，6： | 2 |
| 2 | The change of serum leptin and its relationship with platelet membrane glycoprotein Ib in patients with coronary heart disease显示文摘The aim of this paper was to investigate the change of serum leptin and its relationship with platelet mem-brane glycoprotein Ib(GP Ib)in patients with coronary heart disease(CHD).The enrolled included 50 patients with CHD(CHD group)and 30 patients without CHD(control group)who were diagnosed by coronary angiography.The positive percentage and the average fluorescence intensity of platelet membrane GP Ib were detected by full-blood flow cytometry.Serum leptin was detected by enzyme linked immunosorbent assay.The positive percentage and the average fluorescence intensity of platelet membrane GP Ib in the CHD group were significantly lower than those in the control group(P<0.05).After correcting the differences of systolic blood pressure,body mass index(BMI),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),fasting glucose,PPBS,fasting insulin and quantita-tive insulin sensitive index,serum leptin level in the CHD group was significantly higher than that in the control group(P<0.05).Single factor correlative analysis revealed that serum leptin in CHD patients was negatively correlated with the average fluorescence intensity of platelet membrane GP Ib(P<0.05).Multifactorial stepwise regression analysis showed that serum leptin in CHD patients was independently negatively correlated with the average fluorescence intensity of platelet membrane GP Ib(P<0.05).Logistic analysis demonstrated that serum leptin was independently correlated with the risk of CHD(P<0.05).Hyperleptinemia was veri-fied in CHD patients.The increase of serum leptin could affect blood platelet activation.Hyperleptinemia may play an important role in the pathogenesis of CHD. | XIA Dasheng SONG Yanqiu LI Chao ZHANG Feng WEI Minxin | 2007 | Frontiers of Medicine2007，1，4： | 0 |
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