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| 1 | LSD1 coordinates with the SIN3A/HDAC complex and maintains sensitivity to chemotherapy in breast cancer显示文摘 | Yang Yang Wei Huang Rongfang Qiu Ruiqiong Liu Yi Zeng Jie Gao Yu Zheng Yongqiang Hou Shuang Wang Wenqian Yu Shuai Leng Dandan Feng Yan Wang | 2018 | Journal of Molecular Cell Biology2018，10，4： | 10 |
| 2 | Genome-wide analysis of expression quantitative trait loci(eQTLs)reveals the regulatory architecture of gene expression variation in the storage roots of sweet potato显示文摘Dissecting the genetic regulation of gene expression is critical for understanding phenotypic variation and species evolution.However,our understanding of the transcriptional variability in sweet potato remains limited.Here,we analyzed two publicly available datasets to explore the landscape of transcriptomic variations and its genetic basis in the storage roots of sweet potato.The comprehensive analysis identified a total of 724,438 high-confidence single nucleotide polymorphisms(SNPs)and 26,026 expressed genes.Expression quantitative trait locus(eQTL)analysis revealed 4408 eQTLs regulating the expression of 3646 genes,including 2261 local eQTLs and 2147 distant eQTLs.Two distant eQTL hotspots were found with target genes significantly enriched in specific functional classifications.By combining the information from regulatory network analyses,eQTLs and association mapping,we found that IbMYB1-2 acts as a master regulator and is the major gene responsible for the activation of anthocyanin biosynthesis in the storage roots of sweet potato.Our study provides the first insight into the genetic architecture of genome-wide expression variation in sweet potato and can be used to investigate the potential effects of genetic variants on key agronomic traits in sweet potato. | Lei Zhang Yicheng Yu Tianye Shi Meng Kou Jian Sun Tao Xu Qiang Li Shaoyuan Wu Qinghe Cao Wenqian Hou Zongyun Li | 2020 | Horticulture Research2020，7，1： | 2 |
| 3 | Associations of Residential Greenness with Depression and Anxiety in Rural Chinese Adults显示文摘BACKGROUND Depression and anxiety are top contributors to non-fatal health loss globally.Several studies have indicated the association between residential greenness and mental health.METHOD The participants(n=27,366)were recruited from four counties in Henan Province,China during 2015–2017.Symptoms of depression and anxiety were evaluated using the Patient Health Questionnaire-2(PHQ-2)and the Generalized Anxiety Disorder-2(GAD-2)in the baseline survey.The level of residential greenness during the 3-year period before the baseline survey was assessed using the Moderate Resolution Imaging Spectroradiometer(MODIS)Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index(EVI).The mixed-effect linear regression model was applied to examine the associations of residential greenness with depression and anxiety.RESULTS The results of adjusted models showed that the score of PHQ-2(Dscore and 95%confidence interval[CI])decreased by-0.024(-0.041,-0.006)and-0.022(-0.038,-0.004)with an interquartile range(IQR)increase in NDVI and EVI within a 1,000-m buffer radius,respectively.The score of GAD-2(Dscore and 95%CI)decreased by-0.024(-0.040,-0.006)and-0.028(-0.044,-0.011),in relation to an IQR increase in NDVI and EVI within a 1,000-m buffer radius,respectively.CONCLUSIONS A higher level of residential greenness was significantly associated with lower risk of depression and anxiety in rural areas of Henan Province.Improving residential greenness accessibility may help to promote the mental health of rural populations. | Niu Di Shanshan Li Hao Xiang Yinyu Xie Zhenxing Mao Jian Hou Xiaotian Liu Wenqian Huo Boyi Yang Guanghui Dong Chongjian Wang Gongbo Chen Yuming Guo | 2020 | The Innovation2020，1，3： | 1 |
| 4 | Multiplex CRISPR/Cas9-mediated knockout of soybean LNK2 advances flowering time显示文摘Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of soybean adaptation to diverse latitudes is still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2(LNK2) homeologs of Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs were designed for editing the four LNK2 genes. A transgene-free homozygous quadruple mutant of the LNK2 genes was developed using the CRISPR(clustered regularly interspaced short palindromic repeats)/Cas9(CRISPR-associated protein 9). Under long-day(LD) conditions, the quadruple mutant flowered significantly earlier than the wild-type(WT). Quantitative real-time PCR(q RT-PCR)revealed that transcript levels of LNK2 were significantly lower in the quadruple mutant than in the WT under LD conditions. LNK2 promoted the expression of the legume-specific E1 gene and repressed the expression of FT2 a. Genetic markers were developed to identify LNK2 mutants for soybean breeding.These results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four LNK2 genes shortens flowering time in soybean. Our findings identify novel components in flowering-time control in soybean and may be beneficial for further soybean breeding in high-latitude environments. | Zhaobo Li Qun Cheng Zhuoran Gan Zhihong Hou Yuhang Zhang Yongli Li Haiyang Li Haiyang Nan Cen Yang Linnan Chen Sijia Lu Wenqian Shi Liyu Chen Yanping Wang Chao Fang Liping Kong Tong Su Shichen Li Kun Kou Lingshuang Wang Fanjiang Kong Baohui Liu Lidong Dong | 2021 | The Crop Journal2021，9，4： | 0 |
| 5 | An electrochemical biosensor based on DNA“nano-bridge”for amplified detection of exosomal microRNAs显示文摘Exosomal miRNAs,as potential biomarkers in liquid biopsy for cancer early diagnosis,have aroused widespread concern.Herein,an electrochemical biosensor based on DNA“nano-bridge”was designed and applied to detect exosomal microRNA-21(miR-21)derived from breast cancer cells.In brief,the target miR-21 can specifically open the hairpin probe 1(HP1)labeled on the gold electrode(GE)surface through strand displacement reaction.Thus the exposed loop region of HP1 can act as an initiator sequence to activate the hybridization chain reaction(HCR)between two kinetically trapped hairpin probes:HP2 immobilized on the GE surface and biotin labeled HP3 in solution.Cascade HCR leads to the formation of DNA“nano-bridge”tethered to the GE surface with a great deal of“piers”.Upon addition of avidin-modified horseradish peroxidase(HRP),numerous HRP were bound to the formed“nano-bridge”through biotin-avidin interaction to arouse tremendous current signal.In theory,only a single miR-21 is able to trigger the continuous HCR between HP2 and HP3 until all of the HP2 are exhausted.Therefore the proposed biosensor achieved ultrahigh sensitivity toward miR-21 with the detection limit down to 168 amol/L,as well as little cross-hybridization even at the single-base-mismatched level.Successful attempts were also made in the detection of exosomal miR-21 obtained from the MCF-7 of breast cancer cell line.To our knowledge,this is the first attempt to built horizontal DNA nano-structure on the electrode surface for exosomal miRNAs detection.In a word,the high sensitivity,selectivity,low cost make the proposed method hold great potential application for early point-of-care(POC)diagnostics of cancer. | Jing Zhang Meifeng Hou Guanyu Chen Huifang Mao Wenqian Chen Wenshen Wang Jinghua Chen | 2021 | Chinese Chemical Letters2021，32，11： | 0 |
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