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| 1 | CIMMYT小麦puroindoline基因型的进一步鉴定与分析显示文摘目的籽粒硬度是小麦市场分级和定价的重要依据,影响磨粉和食品加工品质。CIMMYT是我国最重要小麦引种地之一,研究其硬度基因型对我国小麦引种具有重要的应用价值。方法以CIMMYT常用的236份小麦亲本和高代品系为材料,采用改进的颗粒指数法、特异引物的PCR扩增和改进的SDS-PAGE凝胶电泳对其SKCS硬度及其puroindoine基因型进行鉴定和分析。结果202份硬质麦中,165份为PINA蛋白缺失类型;37份为Pinb-D1b类型,其中19份为非CIMMYT材料,18份亲本中均含有非CIMMYT材料。在34份软质麦中,有野生型、Pina-D1a/Pinb-D1i、Pina-D1c/Pinb-D1h、Pina-D1j/Pinb-D1i和Pina-D1a/Pinb-D1j共5种基因型。两种硬质类型中,Pina-D1b类型胚乳硬质程度明显高于(PSI硬度显著低于)Pinb-D1b类型;4种软质类型中,Pina-D1c/Pinb-D1h和Pina-D1a/Pinb-D1i类型胚乳硬质程度显著高于(PSI硬度显著低于)Pina-D1j/Pinb-D1i和Pina-D1a/Pinb-D1a类型。通过分析各类型的puroindoline基因序列发现,Pinb-D1h和Pinb-D1i编码同一种氨基酸,突变后仍表现为软质是因为第28位精氨酸变成色氨酸,其余软质变异类型色氨酸丰富区附近均未发生变化,这可能是导致其胚乳质地仍未变硬的主要原因。结论在先前研究基础上,本试验对CIMMYT小麦基因型作了进一步分析,发现系谱中没有外源材料的所有CIMMYT硬质麦均为Pina-D1b类型。从山羊草引进的软质突变类型之间存在一定硬度差异。 | 陈锋 夏先春 Manila William Morten Lillemo Richard Trethowan Roberto J Peňa 何中虎 | 2006 | 中国农业科学2006，39，8： | 6 |
| 2 | 14-day triple, 5-day concomitant, and 10-day sequential therapies for Helicobacter pylori infection in seven Latin American sites: a randomised trial显示文摘 | E Robert Greenberg Garnet L Anderson Douglas R Morgan Javier Torres William D Chey Luis Eduardo Bravo Ricardo L Dominguez Catterina Ferreccio Rolando Herrero Eduardo C Lazcano-Ponce María Mercedes Meza-Montenegro Rodolfo Pe?a Edgar M Pe?a Eduardo Salazar- | 2011 | The Lancet2011，，9790： | 2 |
| 3 | Immunity:研究开发出最优的HIV疫苗递送模式显示文摘HIV已不再占据头条新闻。这主要是因为抗逆转录病毒药物的成功开发已将HIV病毒感染导致的艾滋病(AIDS)转化为一种慢性的可控制的疾病。然而。当前在全世界大约0.367亿名HIV感染者中,仅约一半的人能够获得控制这种病毒所需的药物。与此同时。新的HIV感染率持续保持在非常高的水平,这突出强调了需要开发一种预防性疫苗。然而,几十年来。 | Matthias Pauthner Colin Havenar-Daughton Devin Sok Joseph P. Nkolola Raiza Bastidas Archana V. Boopathy Diane G. Carnathan Abishek Chandrashekar Kimberly M. Cirelli Christopher A. Cottrell Alexey M. Eroshkin Javier Guenaga Kirti Kaushik Daniel W. Kulp Jinyan Liu Laura E. McCoy Aaron L. Oom Gabriel Ozorowski Kai W. Post Shailendra K. Sharma Jon M. Steichen Steven W. de Taeye Talar Tokatlian Alba Torrents de la Peña Salvatore T. Butera Celia C. LaBranche David C. Montefiori Guido Silvestri Ian A. Wilson Darrell J. Irvine Rogier W. Sanders William R. Schief Andrew B. Ward Richard T. Wyatt Dan H. Barouch Shane Crotty Dennis R. Burton | 2017 | 现代生物医学进展2017，17，27： | 2 |
| 4 | Human aflatoxicosis in developing countries:a review of toxicology,exposure,potential health consequences,and interventions显示文摘 | Williams JH Phillips TD Jolly PE | 2004 | Am J Clin Nutr2004，80，5： | 1 |
| 5 | Side effects of denbuterol as repartitioning agent 显示文摘 | Brockway JM MacRae JC Williams PE | 1987 | Vet Rec1987，120，16： | 1 |
| 6 | Changes in saccomere length and physiological properties in immobilized muscle显示文摘 | Williams PE Goldspink G | 1978 | J Anat1978，127，3： | 1 |
| 7 | A novel serum protein similar to Clq, produced exclusively in adipocytes 显示文摘 | Scherer PE William S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 8 | A novel serum protein similar to Clq, produced exclusively in adipocytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 9 | A novel serum protein similar to C1q,produced exclusively in adipocytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 10 | A novel serum protein similar to C1q, produced exclusively in adipoeytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 11 | A novel serum protein similar to Clq,produced exclusively in adipocytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 12 | A novel serum protein similar to C1q, produced exclucively in adipocytes 显示文摘 | Schere PE William S Foglinano M | 1995 | J Biol chem1995，270，26： | 1 |
| 13 | Zinc-histidine complex protects cultured cortical neurons against oxidative stress-induced damage显示文摘 | Robert J Williams Jeremy PE Spencer | 2004 | Neuroscience Letters2004，371，23： | 1 |
| 14 | Tricholemmal carcinoma:clinicopathologic study of 10 cases显示文摘 | Swanson PE Mrrogi AJ Williams DJ | 1992 | J Cutan Pathol1992，19，2： | 1 |
| 15 | Clinical virology of Ebola hemorrhagic fever (EHF): virus, virus antigen, and IgG and IgM antibody findings among EHF patients in Kikwit, Demo- cratic Republic of the Congo, 1995 显示文摘 | Ksiazek TG Rollin PE Williams AJ | 1999 | J Infect Dis1999，，： | 1 |
| 16 | A novel serumprotein similar to Clqproduced exclusively in adipocytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
| 17 | A novel serum protein similar to Cl q , produced exclusively in adipocytes显示文摘 | Scherer PE Williams S Fogliano M | 1995 | ] Bioi Chern1995，270，26： | 1 |
| 18 | Ebola ( subtype Re- ston) virus among quarantined nonhuman primates recently im- ported from the Philippines to the United States显示文摘 | Rollin PE Williams P J Bressler DS | 1999 | J Infect Dis1999，179，1： | 1 |
| 19 | Changes in sarcomere length and physiological properties in immobilized muscle显示文摘 | Williams PE Goldspink G | 1978 | J Anat1978，127，3： | 1 |
| 20 | A novel serum protein similar to Clq, produced exclusively in adipocytes显示文摘 | Scherer PE Williams S fogliano M | 1995 | J Biol Chem1995，270，26： | 1 |
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