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| 1 | Chromium(VI)-induced Production of Reactive OxygenSpecies, Change of Plasma Membrane Potential and Dissipation of Mitochondria Membrane Potential in Chinese Hamster Lung Cell Cultures显示文摘Objective\ To examine whether Reactive Oxygen Species (ROS) is generated, and whether plasma membrane potential and mitochondrial membrane potential are depolarized in Chinese Hamster Lung (CHL) cell lines exposed to Cr (VI). Methods\ CHL cells were incubated with Cr(VI) at 10 μmol/L, 2.5 μmol/L, 0.65 μmol/L for 3 and 6 hours, respectively. The production of ROS was performed by using 2,7_dichlorofluorescin diacetate; The changes in plasma membrane potential were estimated using fluorescent cationic dye DiBAC4; And the changes in mitochondria membrane potential were estimated using fluorescent dye Rhodamine 123. Results\ The ROS levels in CHL cells increased in all treated groups compared with the control group (P<0.01); The plasma membrane potential and mitochondrial membrane potential in CHL cells dissipated after incubated with Cr(VI) at 10 μmol/L for 3 hours and 6 hours (P<0.01), at 2.5 μmol/L for 6 hours (P<0.01 or 0 05). Conclusion\ Cr(VI) causes the dissipation of plasma membrane potential and mitochondrial membrane potential in CHL cell cultures, and Cr(VI)_induced ROS may play a role in the injuries. | XIEYI ZHUANGZHI-XIONG | 2001 | Biomedical and Environmental Sciences2001，14，3： | 9 |
| 2 | cDNA microarray in isolation of novel differentially expressed genes related to human glioma and clone of a novel full- length gene显示文摘Background This investigation was undertaken to obtain differentially expressed genes related to human glioma using cDNA microarray and the characterization of one novel full-length gene. Methods Total RNA was extracted from human glioma tissues and normal brain tissues, and mRNA was used to make probes. After hybridization and washing, the results were scanned using a computer system. The gene named 681F05 clone was an expressed gene to human glioma through four-time hybridization and scanning. Subsequently northern blot analysis was performed by northern blot, 5’RACE and bioinformatics. Results Fifteen differentially expressed genes to human glioma were obtained through four-time hybridization and scanning. Northern blot analysis confirmed that 681F05 clone was low-expressed in human brain tissues and over-expressed in human glioma tissues. The analysis of BLASTn and BLASTx showed that 681F05 clone is two cDNA clones encoding two novel proteins that are highly identified to the cyclophilin isoform 10 of C. Elgans, respectively. Sequence analysis revealed the two cDNA clones are two different splicing variants of a novel cycophilin-like gene (PPIL3a and PPIL3b).Conclusions cDNA microarray technology can be successfully used to identify differentially expressed genes. The novel full-length gene of human PPIL3 may be correlated with the formation of human glioma. | QIZhen-yu HUIGuo-zhen LIYao ZHOUZong-xiang GUShao-hua YINGKang XIEYi | 2005 | Chinese Medical Journal2005，，10： | 3 |
| 3 | Study on the DME-cytochrome b_5 and its mutants at site of F58显示文摘Phenylalanine-58 is one of the conservative residues in the hydrophobic pocket of Cyt b5, which forms aromatic stacking with the heme b. Previous study showed that both the stacking and the property of the aromatic resi-due affect hydrophobicity of the heme pocket, leading to change of protein抯 property. In order to further reveal the essence we esterify the heme propionate of Cyt b5, F58Y and F58W, and eliminate the hydrogen bond between heme propionate and Ser64 in examining the effect of hydrogen net on the p-p interaction. In this paper thermal denaturation of DME-Cyt b5 and its F58Y and F58W mutants has been stud-ied by UV-visible and CD spectra. The heme transfer reac-tions between these proteins and apo-myoglobin have been studied as well. The results demonstrate that esterification did not destroy the aromatic stacking; however, it affects the stability of the proteins due to different volumes, hydropho-bicities and hydrogen bonds forming ability of these sub-stituents. | SUHui LUJunxia WANGYunhua RENYi XIEYi HUANGZhongxian | 2004 | Chinese Science Bulletin2004，49，18： | 1 |
| 4 | The analysis of researchand development to the hazardous waste disposal technology in ourcountry显示文摘 | XIEYi(谢毅) HAOHaisong(郝海松) | 2008 | 化学工程与装备2008，，12： | 1 |
| 5 | LignansfromKadsuraAngustifolia显示文摘 | ChenYG QinGW XieYY etal | 1998 | JAsianNatProdRes1998，1，2： | 1 |
| 6 | GrowthofSb2E3(ES, Se) polygonal tubular crystals via a novel solvent-relief-selfseeding process显示文摘 | ZhengXiuwen XieYi ZhuLiying | 2002 | Inorg Chem2002，41，3： | 1 |
| 7 | The Transcriptional Profile in the Parvovirus H1sensitive Liver Cells显示文摘To gain wholesale information about antineoplastic mechanism of parvovirus, a complementary DNA microarray, representing 12800 different genes, was used to compare the gene expression variance between a H1 sensitive human liver cell line, L02, and a resistant derivative clone, L02R, which survived the infection of 10 m.o.i, parvovirus H1. The prepared cDNAs of L02 and L02R were labeledwith fluorescent dyes, Cy5 and Cy3 respectively, with which the mixed sample gave a red and a green image after hybridization. The two images were merged into one for further analyses. | HuangQing-shan GuMei-gang GuoLan-ping LiYao XieYi LuoZu-yu | 2000 | 胃肠病学2000，5，B08： | 0 |
| 8 | Effect of Transfection with p53 Gene on Malignancy of Human Hepotacellular Carcinoma Cell Lines显示文摘Mutations in p53 gene occur in more than 50% of human tumor cells. Introduction of exogenous wild type p53 gene into tumor cells in vitro often leads to the reduction of the latter's malignancy and tumor formation ability in nude mice. QGY-7703 and SMMC-7721 are two human hepatoceUular carcinoma cell lines established in China. Researchers in our laboratory had investigated their p53 backgrounds in the earlier works. | XuHong LiJian-hong GuoLan-ping XieYi LuoZu-yu | 2000 | 胃肠病学2000，5，B08： | 0 |
| 9 | Tumor Gene Therapy Vectors Based on Parvovirus H-1显示文摘Parvovirus H-1 has been a promising vector in human gene therapy of tumor. With a vector, pSHll8, a mutation was performed down the site of p38 promoter to change ATG codon into ACG, which leaves the NS 1 protein intact and ensures correct expression of the downstream report genes, such as enhanced green fluorescent protein (GFP) gene and wt p53 cDNA, in newly constructed H1 based vectors (pH1EG and pHI/p53). The vector, pH1EG, | HuangQing-shan LiLi GuMei-gang GuoLan-ping LuoZu-yu XieYi | 2000 | 胃肠病学2000，5，B08： | 0 |
| 10 | Rituximab in combination with cyclophosphamide, vincristine, doxorubicin and prednisone for treatment of initially diagnosed diffuse large B cell lymphoma: a multi-center clinical study显示文摘AIM: To evaluate the efficacy of rituximab combined with cyclophosphamide, vincristine, doxorubicin, and prednisone (CHOP) in treating the initially diagnosed diffuse large B cell lymphoma (DLBL). METHODS: From Apr.2002 to Feb. 2003, 52 patients were enrolled in this study. Chemotherapy was conducted with cyclophosphamide 600 mg·m^-2, vincristine 1.4 mg·m^-2,doorubicin 25mg·m^-2 on d 1 and prednisone 60 mg·d^-l for successive 5 d (standard CHOP). There were 6 courses, 3 wk each. Rituximab 375 mg·m^-2 was infused once a week, 2 d before the first course of chemotherapy (successive infusion) for 4 times on standard dose or for 6 times on extended dose. Or rituximab was infused once every 3 wk, 2 d before each CHOP (separated infusion) for 4 times on the schedule of standard dose or for 6 times on the extended dose. RESULTS: The complete response (CR) rate (60%) and total effective (100%) were achieved in 50 patients who were evaluated for efficacy, respectively. And among 34 patients in Ann Arbor stage Ⅲ and Ⅳ, 15 patients were completely relieved. The complete effective rate was 44%. Fifty patients were followed-up for (8±s 5) wk, 2-30wk and estimated progress free survival (PFS) rate of 16 wk was 87 %. Standard and extend regimen were not different in effect, as well as the separated or concentrated infusion of rituximab (P>0.05). The regimen could be well tolerated, and the major adverse reactions were infusion-related response (32 % ) and hematological toxicities (20 %). CONCLUSION:Rituximab in combined with CHOP can be successfully applied to the therapy of initially diagnosed diffuse large B cell lymphoma, with high CR rate and mild adverse reactions. | LIJun-min SHENYang CHENFang-yuan XIEYi WANGChun HOUJian HONGXiao-nan WUDe-pei CHENJia HOUMing XUJian-min SHENZhi-xiang | 2004 | 中国新药与临床杂志2004，23，1： | 0 |
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