| 1 | Somatic hybridization between rice and ap.omictic Panicum显示文摘Protoplasts from suspension-culture cells of japonica rice cultivar 02428 (Oryza sativa L.)were inactivated with 2.5 mM indoacetamide (IDA) at room temperature (22-25℃) for 15 minutes before fusion. The nonmorphogenic protoplasts from suspension culture of apromictic Panicum maximum Jacq. were treated with 60 Kr soft X-ray or with no treat ment. These inactivated protoplast mixtures were fused by electrofusion or PEG method and incubated. After incubation, over 50 putative rice cybrids plantlets were obtained. A mong them, 28 regenerated plants were vigorously growing and flowering. Comparing with the original rice parental plants, these fusion-cell derived plants have remarkahle variation in morphology and structure on the flower organ. In most plants, the number of anthers was increased, ranging from 7 to 13 and the number of stigma or ovary was increased, ranging from 3 to 6 on a single flower(see fingure). In some plants, the fiumber of ovule in a single ovary or the embryo sac in a single ovule | YANQiusheng ZHANGXueqin TENGSheng SUNJingsan XINHuawei | 1995 | Chinese Rice Research Newsletter1995，3，4： | 0 |
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