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| 1 | Detection of micrometastasis of gastric carcinoma in peripheral blood circulation显示文摘AIM:To detect the micrometastasis of gastric carcinoma in peripheral blood circulation using immunomagnetic beads sorting technique and RT-PCR technique,and to discuss its significance and the difference between the two methods.METHODS:Density gradient centrifugation was used to isolate mononuclear cells from peripheral blood,immunomagnetic beads sorting technique and RT-PCR technique were used to detect the disseminated carcinoma cells.HE,immunocytochemical and immunofluorescence staining were also used to identify the characteristics of the cells separated with immunomagnetic beads sorting technique.RESULTS:Cells expressing cytokeratin were separated and enriched from the peripheral blood specimens of patients suffering from gastric carcinoma or chronic gastritis. After HE staining, two kinds of ceils with little cytoplasm were found.Majority of these cells had small and round nuclei, even chromatins and the thickness of nuclear membrane was normal. Immunohistochemical staining indicated that there were CD34 and CD45 expression on the cell membrane ofthis kind of cells and these cells also showed expressed human telomerase reverse transcriptase by immunofluorescence staining, but the expression of carcinoembryonic antigen was absent.So,these cells might hematopoiesis precursors.Another kind of cells had larger and abnormal nuclei with thicker nuclear membranes. Massed chromatins and polynudeoli were found in the nudei. These cells expressed human telomerase reverse transcriptase and carcinoembryonic antigen,but CD34 and CD45 were not found on the cell membrane.So,these cells were considered as gastric carcinoma cells escaping from the original focuses and existing in the peripheral blood circulation.Cardnoma cells were found in 25 of 60(41.7%) specimens of peripheral blood from patients with gastric carcinoma, while there were no such cells separated from the blood specimens of chronic gastritis patients.The difference of positive rates of disseminated carcinoma cells between two groups was markedly significant (P<0.005).The expressions of CK20 mRNA in peripheral blood specimens were examinated withRT-PCR. CK20 mRNA was detected from 32 of 60(53.3%) peripheral blood specimens in the group of gastric carcinoma patients,while none of the specimens from patients suffering from chronic gastritis had CK20 mRNA. Significant difference was also found between two groups (P<0.005).Statistic analyses also showed that there was a significant difference between the positive rates of two methods in detecting the disseminated carcinoma cells from the peripheral blood circulation of gastric carcinoma patients (P<0.05).CONCLUSION:The results demonstrated that there were disseminated carcinoma cells in the peripheral blood circulation of some patients with gastric carcinoma.Disseminated carcinoma cells can be detected from the peripheral blood samples with immunomagnetic beads sorting technique and RT-PCR technique.The positive rate of RT-PCR technique is higher than that of immunomagnetic beads sorting technique in detecting micrometastasis. | Xi-MeiChen Guo-YuChen Zhi-RongWang Feng-ShangZhu Xiao-LeiWang XiaZhang | 2004 | World Journal of Gastroenterology2004，10，6： | 32 |
| 2 | TIP30 regulates apoptosis-related genes in its apoptotic signal transduction pathway显示文摘AIM: To investigate the role of TIP30 in apoptotic signal pathway in hepatoblastoma cells and to provide a basis for TIP30 as a gene therapy candidate in the regression of hepatoblastoma cells.METHODS: Apoptosis of human hepatoblastoma cell lines HepG2 (p53 wild), Hep3B (p53 null) and PLC/RPF/5 (p53mutant) infected with Ad-TIP30 (bearing a wild type human Tip30 gene) were analyzed and p53, Bax and Bclxl expression levels were compared among these cells.MlT assay, DNA fragmentation, in situ 3' end labeling of DNA, annexin-Ⅴ FITC staining were used to detect cell death and apoptosis in cells at various time intervals subsequent to infection, and to determine whether TIP30 had an effect on the expression levels of some apoptosis-related gene products such as Bax, p53 and Bcl-xl. A similar time course experiment was performed by Western blotting.RESULTS: In MTT assay, the viability of HepG2 cells decreased significantly from 99.7% to 10% and displayed more massive cell death within 5-8 d than Hep3B and PLC/RPF/5 cells, with their viability decreased from 97.8% to 44.3% and 98.1% to 50.4%, respectively. In annexin-ⅤFITC assay, the percentage of apoptosis cells in HepG2cells was two to three-fold higher than that in control cells (infected with Ad-GFP), two-fold higher than that in Hep3B cells and 1.4-fold higher than that in PLC/RPF/5 cells 36 h after infection, respectively. Moreover, in HepG2 cells, the p53 began to increase 6-8 h after infection, reaching a maximum level between 8 and 12 h after infection and then dropped. Bax showed a similar increase in the cells as p53 reached the maximum at 8-12 h and subsequently decreased. Interestingly, Bcl-xl protein levels were down regulated during 24 to 36 h after Ad-TIP30 infection. In contrast, ectopic expression of TIP30 in Hep3B and PLC/RPF/5 cells had no effect on the regulation of Bax expression, but had an effect on Bcl-xl levels. In comparison with HepG2 cells, these data suggested that up-regulation of p53 levels by TIP30 might be a pre-requisite for Bax and Bax/Bcl-xl ratio increase. We hypothesized that TIP30 might regulate Bax gene partly through p53, which sensitizes cells to apoptosis by involving a p53 apoptosis signal transduction pathway.CONCLUSION: TIP30 plays an important role in predisposing hepatoblastoma cells to apoptosis through regulating expression levels of these genes. Ad-TIP30carrying exogenous TIP30-anti-tumor genes may be regarded as a potential candidate for the treatment of hepatocellular carcinoma. | MeiShi XiaZhang PingWang Hong-WeiZhang Bai-HeZhang Meng-ChaoWu | 2005 | World Journal of Gastroenterology2005，11，2： | 22 |
| 3 | Effect of rainfall interannual variability on the biomass and soil water distribution in a semiarid shrub community显示文摘The dynamics of biomass and soil moisture in semiarid land is driven by both the current rainfall and the ecosystem memory.Based on a meta-analysis of existing experiments,an ecosystem model was used to calculate the effect of the rainfall interannual variability on the pattern of biomass and soil moisture in a shrub community.It was found that rainfall interannual variability enabled shrubs to be more competitive than grasses,and to maintain the dominant role over a longer time.The rainfall interannual variability resulted in complex soil moisture dynamics.The soil water recharge in wet years alternated with discharge in drought years. | LIU JunShanXU XiaZHANG Yong,TIAN YuQiang & GAO Qiong State Key Laboratory of Earth Surface Processes and Resource Ecology,College of Resources Science & Technology,Beijing Normal University,Beijing 100875,China | 2010 | Science China(Life Sciences)2010，53，6： | 7 |
| 4 | The Grape Component Resveratrol Interferes with the Function of Chemoattractant Receptors on Phagocytic Leukocytes显示文摘Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory and anti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of G protein-coupled chemoattractant receptors, which play important roles in inflammation and immune responses.RV, over a non-cytotoxic concentration range, inhibited chemotactic and calcium mobilization responses of phagocytic cells to selected chemoattractants. At low micromolar concentrations, RV potently reduced superoxide anion production by phagocytic leukocytes in response to the bacterial chemotactic peptide fMLF, a high affinity ligand for formylpeptide receptor FPR, and Aβ42, an Alzheimer's disease-associated peptide and a ligand for the FPR variant FPRL1. In addition, RV reduced phosphorylation of extracellular signal-regulated kinase (ERK1/2) and the activation of nuclear factor NF-κB induced by formylpeptide receptor agonists. These results suggest that the inhibition of the function of chemoattractant receptors may contribute to the anti-inflammatory properties of RV. Thus, RV may be therapeutically promising for diseases in which activation of formylpeptide receptors contributes to the pathogenic processes. Cellular & Molecular Immunology. 2004;1(1):50-56. | HengyiTao ChunfuWu YeZhou WanghuaGong XiaZhang PabloIribarren YuqingZhao YingyingLe JimingWang | 2004 | Cellular & Molecular Immunology2004，1，1： | 6 |
| 5 | La1-xCexMnO3/attapulgite nanocomposites as catalysts for NO reduction with NH3 at low temperature显示文摘 | Xiazhang Li YU Yin Chao Yao Shixiang Zuo Xiaowang Lu Shiping Luo Chaoying Ni | 2016 | Particuology2016，14，3： | 4 |
| 6 | Facile synthesis of Ce02 nanotubes templated by modified attapulgite 显示文摘 | Chen Zhigang Chen Feng Li Xiazhang | 2010 | Journal of Rare Earths2010，28，4： | 1 |
| 7 | Connexin 43 Reverses Malignant Phenotypes of Glioma Stem Cells by Modulating E‐Cadherin显示文摘 | Shi‐CangYu Hua‐LiangXiao Xue‐FengJiang Qing‐LiangWang YanLi Xiao‐JunYang Yi‐FangPing Jiang JieDuan Jian‐YongJiang Xian‐ZongYe Sen‐LinXu Yang‐HongXin Xiao‐HongYao Jian‐HongChen Wei‐HuaChu WeiSun BingWang Ji MingWang XiaZhang Xiu‐WuBian | 2012 | STEM CELLS2012，，2： | 1 |
| 8 | Oriented polycrystalline mesoporous CeO 2 with enhanced pore integrity显示文摘 | Chaoying Ni Xiazhang Li Zhigang Chen Hui-Yin Harry Li Xinqiao Jia Ismat Shah John Q. Xiao | 2008 | Microporous and Mesoporous Materials2008，，3： | 1 |
| 9 | Upconversion hollow nanospheres CeF_(3) co-doped with Yb^(3+) and Tm^(3+) for photocatalytic nitrogen fixation显示文摘Solar driven nitrogen(N_(2))fixation to synthesize ammonia is a potential alternative for the traditional Haber-Bosch approach to meeting industrial demand,but is largely hampered by the difficulties in the harvesting of solar energy and activating inert N_(2).In this work,hollow CeF_(3) nanospheres co-doped with activator Tm^(3+)and sensitizer Yb^(3+)(Yb^(3+):Tm^(3+):CeF_(3))were prepared by microwave hydrothermal method.The product was employed as a catalyst for photo-driven N_(2) fixation by adjusting the molar ratio of Ce^(3+):Yb^(3+):Tm^(3+).Results show that the porous hollow structure enhances the light-harvesting by physical scattering and reflection.In addition,heteroatom doping generates abundant fluorine vacancies(F_(V))which provide abundant active sites for adsorption and activation of N_(2).The sample with molar ratio of CeF_(3):Yb^(3+):Tm^(3+)at 178:20:2 demonstrates the highest utilization of solar energy attributed to the strongest upconversion capability of near-infrared(NIR)light to visible and ultraviolet(UV)light,and the NH_(4)+concentration achieves the highest value of 15.06μmol/(gcat∙h)under simulated sunlight while nearly 6.22μmol/(gcat∙h)under NIR light.Current study offers a promising and sustainable strategy for the fixation of atmospheric N_(2) using full-spectrum solar energy. | Minghui Zhong Zhendong Wang Da Dai Baozhu Yang Shixiang Zuo Chao Yao Fengqin Wu Xiazhang Li | 2022 | Journal of Rare Earths2022，40，4： | 1 |
| 10 | Layer-by-layer synthesis of hollow spherical CeO2 templated by carbon spheres显示文摘 | Xiazhang Li Feng Chen Xiaowang Lu Chaoying Ni Xiaobing Zhao Zhigang Chen | 2010 | Journal of Porous Materials2010，，3： | 1 |
| 11 | Support vector machines and kd‐tree for separating quasars from large survey data bases显示文摘 | DanGao Yan‐XiaZhang Yong‐HengZhao | 2008 | Monthly Notices of the Royal Astronomical Society2008，，3： | 1 |
| 12 | Microwave hydrothermal synthesis of lanthanum oxyfluoride nanorods for photocatalytic nitrogen fixation:Effect of Pr doping显示文摘Photocatalytic fixation of nitrogen has been recognized as a green and promising strategy for ammonia synthesis under ambient conditions.However,the efficient reduction of nitrogen remains a challenge due to high activation energy of nitrogen and low utilization of solar energy.Herein,lanthanum oxyfluoride with different doping content of Pr3+(LaOF:xPr3+)upconversion nanorods were synthesized by microwave hydrothermal method.Results indicated that the doping of Pr3+generated considerable defects on the surface of LaOF which acted as the adsorption and activation center for nitrogen.Meanwhile,the Pr3+ion narrowed the band gap and broadened the light response range of LaOF because LaOF:Pr3+can upconvert visible light into ultraviolet light,which excite LaOF nanorods and improve the utilization of solar light.The doping amount of Pr3+had critical effect on the photocatalytic nitrogen fixation performance which reached as high as 180μmol·L?1·ho1 when the molar ratio of Pr3+to LaOF was optimized to be 2%. | Xiangyu Yan Da Dai Kun Ma Shixiang Zuo Wenjie Liu Xiazhang Li Chao Yao | 2020 | Frontiers of Materials Science2020，14，1： | 0 |
| 13 | Construction of upconversion fluoride/attapulgite nanocomposite for visible-light-driven photocatalytic nitrogen fixation显示文摘Developing photocatalysts with wide spectrum absorption and strong nitrogen activation is critical for nitrogen fixation under mild conditions.Herein,one-dimensional natural clay attapulgite(ATP)supported YFg:Sm^3+were successfully synthesized via microwave hydrothermal method,and the composites were employed as the catalyst for photocatalytic nitrogen fixation under visible-light irradiation.Results indicated that the production of ammonia reached as high as 41.2 mg·L^-1 within 3 h when the molar ratio of Sm^3+and the mass fraction of YF3:Sm^3+were optimized.The enhanced fixation performance is mainly due to that the modified ATP fibber with abundant active sites and the doped fluoride with defective vacancy facilitate the adsorption and activation of N2.Furthermore,the upconversion property of YF3:Sm^3+increases the harvesting of visible-light energy,meanwhile the Z-scheme heterostructure built between YF:Sm^3+and modified ATP inhibits the recombination of charge carriers and retains high redox potentials for N2 reduction. | Xuhua YE Xiangyu YAN Xini CHU Shixiang ZUO Wenjie LIU Xiazhang LI Chao YAO | 2020 | Frontiers of Materials Science2020，14，4： | 0 |
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