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您的检索式：作者名="Xueliang Dai"
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| 1 | Role of JAK-STAT3 signaling pathway during neuronal differentiation of rat bone marrow mesenchymal stem cells显示文摘BACKGROUND: Recent studies regarding neuronal differentiation of mesenchymal stem cells (MSCs) have primarily focused on induction methods and transplantation in vivo.However,knowledge about the intrinsic regulatory mechanisms underlying neuronal induction of MSCs remains limited and unclear.OBJECTIVE: To elucidate the role of JAK-STAT3 signaling pathway during neuronal differentiationof MSCs using a combination of the JAK-STAT3 signaling inhibitor AG490 and growth factors.DESIGN,TIME AND SETTING: Neural,molecular,biomedical,in vitro experiment was performed at the Laboratory of Pharmacology,School of Pharmacy,Nanjing Medical University between Marchand December 2008 MATERIALS: An inhibitor of the JAK-STAT3 signaling pathway was purchased from Calbiochem,USA.Antibody kit for total and phosphorylated STAT3 was purchased from Cell Signaling,USA.METHODS: MSCs from passage 3 were assigned to non-induced,growth factor,and AG490 groups.MAIN OUTCOME MEASURE: The number of cells expressing neuron-specific enolase,microtubule-associated protein,and glial fibrillary acidic protein were determined by immunocytochemistry.Total and phosphorylated (Tyr705) expression levels of STAT3 protein were measured by Western blot analysis.RESULTS: MSCs were transdifferentiated into neuronaland astrocyte-like phenotypes throughthe induction of epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor.In addition,the JAK-STAT3 signaling pathway was significantly activated during neural differentiation.Expression of phosphorylated (Tyr705) STAT3 was inhibited with AG490 (5 μmol/L) prior to neural induction with epidermal growth factor,basic fibroblast growth factor,and brain-derived neurotrophic factor;proportion of astrocyte-like cells was significantly reduced (P < 0.01),and the proportion of neuronal-like phenotypes was significantly increased (P < 0.01).CONCLUSION: JAK-STAT3 signaling pathway was shown to regulate neuronal induction of bone marrow MSCs.The proportion of MSC-induced neuronal-like cells was increased following treatment with the JAK-STAT3 signaling inhibitor AG490. | Huaiyong Hao Yongguang Wang Feng Cheng Baosheng Huang Heping Tian Xueliang Dai Lixin Li | 2010 | Neural Regeneration Research2010，5，5： | 2 |
| 2 | Molecular Characterization and Virulence Genes of Aeromonas hydrophila Isolated from the Chinese Giant Salamander (Andrias davidianus)显示文摘The Chinese giant salamander(Andrias davidianus) is the largest living amphibian in the world. Aeromonas hydrophila strain L602 was isolated from A. davidianus. The 16S rDNA gene of this isolate was amplified using PCR,and the phylogenetic tree was constructed by the neighbor-joining method. Four virulence genes(aerA,aha1,hly and alt) of A. hydrophila were amplified by PCR and drug resistances were tested using Kirby-Bauer disk diffusion method. The results showed that the length of this 16S rDNA sequence was 1453 bp,which showed 99% homology with A. hydrophila. The GenBank accession number was JX155398. Phylogenetic analysis indicated it grouped together with A. hydrophila. Four virulence genes were all detected,indicating that strain L602 was highly virulent. This stain was resistant to four antibiotics(vibramycin,furazolidone,ampicillin and erythromycin),while it was insensitive to streptomycin. Furthermore,this strain was susceptible to six antibiotics(sulfafurazole,ciprofloxacin,penbritin,norfloxacin,florfenicol and enrofloxacin). This study will help to validate the classification and virulence of pathogenic bacteria in amphibians. | Li WANG Yong WEI Guiping YUAN Min DAI Xueliang CHEN | 2012 | Asian Herpetological Research2012，3，4： | 1 |
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