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| 1 | Cloning, expression, and characterization of a novel diketoreductase from Acinetobacter baylyi显示文摘oxidoreductases 催化的羰基组的减小涉及所有生物过程并且经常是重要 biocatalyst 的一个班。在这篇文章,我们报导指定为 diketoreductase (DKR ) 能与优秀 stereoselectivity 在 diketo 酉旨把二个羰基组归结为相应 dihydroxy 酉旨的新奇的酶。DKR 被反向的基因方法从 Acinetobacter baylyi 克隆,不同类地在 Escherichia coli 表示了,并且净化了到由二色析法的步的同质。这新奇的酶展出了双余因子特性,与在 NADPH 上的 NADH 的偏爱。DKR 催化的 dihydroxy 酉旨产品仅仅是有两 diastereomeric 过量的 3R,5S 立体异构, enantiomeric 过量珍视超过 99.5% 。另外,酶的一些生物化学的性质例如最佳的 pH 和温度,也被描绘。而且,顺序分析显示这新酶对细菌的 3-hydroxyacyl 辅酶相应 -- 脱氢酶。基于唯一的催化活动和优秀 stereoselectivity,更重要地, DKR 能在珍贵 chiral 药中介的合成被利用,例如 Lipitor ? 。 | Xuri Wu Nan Liu Yunmian He Yijun Chen | 2009 | Acta Biochimica et Biophysica Sinica2009，41，2： | 3 |
| 2 | VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway显示文摘Although VEGF-B was discovered as a VEGF-A homolog a long time ago,the angiogenic effect of VEGF-B remains poorly understood with limited and diverse findings from different groups.Notwithstanding,drugs that inhibit VEGF-B together with other VEGF family members are being used to treat patients with various neovascular diseases.It is therefore critical to have a better understanding of the angiogenic effect of VEGF-B and the underlying mechanisms.Using comprehensive in vitro and in vivo methods and models,we reveal here for the first time an unexpected and surprising function of VEGF-B as an endogenous inhibitor of angiogenesis by inhibiting the FGF2/FGFR1 pathway when the latter is abundantly expressed.Mechanistically,we unveil that VEGF-B binds to FGFR1,induces FGFR1/VEGFR1 complex formation,and suppresses FGF2-induced Erk activation,and inhibits FGF2-driven angiogenesis and tumor growth.Our work uncovers a previously unrecognized novel function of VEGF-B in tethering the FGF2/FGFR1 pathway.Given the anti-angiogenic nature of VEGF-B under conditions of high FGF2/FGFR1 levels,caution is warranted when modulating VEGF-B activity to treat neovascular diseases. | Chunsik Lee Rongyuan Chen Guangli Sun Xialin Liu Xianchai Lin Chang He Liying Xing Lixian Liu Lasse DJensen Anil Kumar Harald FLanger Xiangrong Ren Jianing Zhang Lijuan Huang Xiangke Yin JongKyong Kim Juanhua Zhu Guanqun Huang Jiani Li Weiwei Lu Wei Chen Juanxi Liu Jiaxin Hu Qihang Sun Weisi Lu Lekun Fang Shasha Wang Haiqing Kuang Yihan Zhang Geng Tian Jia Mi Bi-Ang Kang Masashi Narazaki Aaron Prodeus Luc Schoonjans David MOrnitz Jean Gariepy Guy Eelen Mieke Dewerchin Yunlong Yang Jing-Song Ou Antonio Mora Jin Yao Chen Zhao Yizhi Liu Peter Carmeliet Yihai Cao Xuri Li | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
| 3 | Maternal zinc alleviates tert-butyl hydroperoxide-induced mitochondrial oxidative stress on embryonic development involving the activation of Nrf2/PGC-1αpathway显示文摘Background Mitochondrial dysfunction induced by excessive mitochondrial reactive oxygen species(ROS)damages embryonic development and leads to growth arrest.Objective The purpose of this study is to elucidate whether maternal zinc(Zn)exert protective effect on oxidative stress targeting mitochondrial function using an avian model.Result In ovo injected tert-butyl hydroperoxide(BHP)increases(P<0.05)hepatic mitochondrial ROS,malondialdehyde(MDA)and 8-hydroxy-2-deoxyguanosine(8-OHdG),and decreases(P<0.05)mitochondrial membrane potential(MMP),mitochondrial DNA(mtDNA)copy number and adenosine triphosphate(ATP)content,contributing to mitochondrial dysfunction.In vivo and in vitro studies revealed that Zn addition enhances(P<0.05)ATP synthesis and metallothionein 4(MT4)content and expression as well as alleviates(P<0.05)the BHP-induced mitochondrial ROS generation,oxidative damage and dysfunction,exerting a protective effect on mitochondrial function by enhancing antioxidant capacity and upregulating the mRNA and protein expressions of Nrf2 and PGC-1α.Conclusions The present study provides a new way to protect offspring against oxidative damage by maternal Zn supplementation through the process of targeting mitochondria involving the activation of Nrf2/PGC-1αsignaling. | Liang Huang Wei Gao Xuri He Tong Yuan Huaqi Zhang Xiufen Zhang Wenxuan Zheng Qilin Wu Ju Liu Wence Wang Lin Yang Yongwen Zhu | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 0 |
| 4 | Arylene Diimide Derivatives as Anolyte Materials with Two-Electron Storage for Ultrastable Neutral Aqueous Organic Redox Flow Batteries显示文摘Two-electron neutral aqueous organic redox flow batteries(AORFBs)hold more promising applications in the power grid than one-electron batteries because of their higher capacity.However,their development is strongly limited by the structural instability of the highly reduced species.By combining the extendedπ-conjugation structure of the anolytes and the enhanced aromaticity of the highly reduced species,we reported a series of highly conjugated and inexpensive arylene diimide derivatives(NDI,PDI,and TPDI)as novel two-electron storage anolyte materials for ultrastable AORFBs.Matched with(ferrocenylmethyl)trimethylammonium chloride(FcNCl)as catholyte,arylene diimide derivative-based AORFBs showed the highest stability in two-electron AORFBs to date.The NDI/FcNCl-based AORFB delivered 98.44%capacity retention at 40 mA cm^(−2)for 350 cycles;TPDI/FcNCl-based AORFB also showed remarkable stability with 97.22%capacity retention at 20 mA cm^(−2)over 200 cycles.This finding lays the theoretical foundation and offers a reference for improving the stability of two-electron AORFBs. | Xu Liu Xuri Zhang Chaoyu Bao Zengrong Wang Heng Zhang Guoping Li Ni Yan Ming-Jia Li Gang He | 2023 | CCS Chemistry2023，5，10： | 0 |
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