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| 1 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 2 | RESTplus: an improved toolkit for resting-state functional magnetic resonance imaging data processing显示文摘Resting-state functional magnetic resonance imaging (RS-fMRI)[1,2] provides relatively high spatial and temporal resolution for mapping spontaneous brain activity non-invasively. It has been widely used in cognitive neuroscience and clinical studies. A number of comprehensive software packages have been developed for RS-fMRI data analysis, among which a MATLAB package named REST (RESing-state fMRI data analysis Toolkit, released in October 2008 at http://www.restfmri.net)[3] is the earliest one dedicated to RS-fMRI analysis. REST focuses on RS-fMRI postprocessing metrics. | Xi-Ze Jia Jue Wang Hai-Yang Sun Han Zhang Wei Liao Ze Wang Chao-Gan Yan Xiao-Wei Song Yu-Feng Zang | 2019 | Science Bulletin2019，64，14： | 14 |
| 3 | Protective Effect of Hydroxysafflor Yellow A on Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Rats显示文摘Objective: To observe the effect of hydroxysafflor yellow A(HSYA), an active ingredient of a traditional Chinese herbal medicine Carthamus tinctorius L., on lung inflammation and pulmonary fibrosis induced by bleomycin(BLM) in rats. Methods: Animals were divided into 6 groups including normal group, model group, three HSYA groups and dexamethasone(DXM) group. Three doses of HSYA(35.6, 53.3, and 80.0 mg·kg^(–1)·day^(–1)) were intraperitoneally(i.p.) injected in rats for 3 weeks after BLM administration and DXM was used as the positive control(n=8 or 12). Arterial blood gas was assayed and morphological changes were observed. Lung mRNA expressions of tumor necrosis factor(TNF)-α, interleukin(IL)-1β, IL-6 and some cytokines in lung tissue were detected by real-time polymerase chain reaction. Nuclear factor-κB p65 or α-smooth muscle actin(α-SMA) protein distribution in rat lung tissue was observed by immunohistochemistry. Results: On the 7 th day after BLM administration, lung tissue showed serious inflammation. Treatment with HSYA or DXM ameliorated lung inflammation. After treatment with HSYA or DXM, oxygen partial pressure(PaO_2) increased(HSYA 80.0 mg·kg^(–1), P<0.01) and CO_2 partial pressure(PaCO_2) decreased(HSYA 53.3, 80.0 mg·kg^(–1), P<0.05). Moreover, the mRNA expression of TNF-α, IL-1β, and IL-6; and the number of NF-κB p65 positive cells was lower in HSYA 53.3 and 80.0 mg·kg^(–1) groups than those in the model group(all P<0.05). Twenty-one days after BLM administration, HSYA or DXM treatment ameliorated fibrosis, increased PaO_2(HSYA 53.3, 80.0 mg·kg^(–1), P<0.01), and decreased PaCO_2(53.3 and 80.0 mg·kg^(–1), P<0.05). Further, the mRNA expression of TGF-β1, α-SMA, and collagen Ⅰ as well as the number of α-SMA positive cells increased in the model group and HSYA can attenuate these changes(53.3, 80.0 mg·kg^(–1), P<0.05). Hematoxylin and eosin and Masson's trichrome staining indicated that the fibrosis and collagen deposition were ameliorated in HSYA groups(53.3, 80.0 mg·kg^(–1), P<0.05). Conclusion: HSYA could alleviate acute lung inflammation and chronic pulmonary fibrosis induced by BLM in rats. | JIN Ming WANG Lin WU Yan ZANG Bao-xia TAN Li | 2018 | Chinese Journal of Integrative Medicine2018，24，1： | 12 |
| 4 | Ultra-sensitive graphene strain sensor for sound signal acquisition and recognition显示文摘为健全信号获得和识别的一个便携、高精确的传感器从编织的特殊设计的 graphene 的薄电影被制作织物(GWF ) 。在被拉长之上,随机的裂缝的高密度出现在网络,它减少当前的小径,从而增加电阻。因此,这部电影能对人的喉咙作为一个紧张传感器起作用以便测量通过肌肉运动,一个声音是否被生产的讲话。超离频敏感允许由提取声波的签名特征的快速、低频率的讲话采样的实现。在这研究, 26 封英语信,典型汉字和音调的代表性的信号,甚至短语和句子被测试,揭示在电阻的明显、典型的变化。而且, graphene 传感器的电阻变化与预告录下的声音完美地反应了。由把人工智能与数字信号处理相结合,我们期望以后,这个 graphene 传感器将能成功地达成复杂声学的系统和听觉的数据的大数量。 | Yan Wang Tingting Yang Junchao Lao Rujing Zhang Yangyang Zhang Miao Zhu Xiao Li Xiaobei Zang Kunlin Wang Wenjian YU Hu Jin Li Wang Hongwei Zhu | 2015 | Nano Research2015，8，5： | 8 |
| 5 | Acquired immunodeficiency syndrome associated disseminated Penicillium Marneffei infection: report of 8 cases显示文摘 | LU Pu-xuan ZHU Wen-ke LIU Yan CHEN Xin-chun ZHAN Neng-yong LIU Jin-qing ZANG Jian YANG Gen-dong YE Ru-xin CAI Li-sheng | 2005 | Chinese Medical Journal2005，，16： | 7 |
| 6 | Gram-scale synthesis of all-inorganic perovskite quantum dots with high Mn substitution ratio and enhanced dual-color emission显示文摘Mn-doped all-inorganic perovskite quantum dots (QDs) provide prominent applications in the fields of low-cost light source or display,because of their remarkable properties including dual-color emission and reduced lead content,as well as high photoluminescence quantum yields (PLQYs) and high stability.However,the existing synthesis approaches usually require hash conditions,such as high temperature and nitrogen protection,which is a major hurdler for the practical manufacturing.In addition,the significantly high Mn substitution ratio in CsPbX3 QDs is still challenging.The real dual-color emission with two strong emission peaks in the Mn-doped all-inorganic perovskite QDs has attracted great interest.Here we present a gram-scale approach to synthesize both CsPbxMn1-xCI3 and CsPb1-xMnxClyBr3-y QDs at 100 ℃ in the air with high Mn substitution ratio,up to 55.64% atomically.The as-prepared CsPb1-xMnxClyBr3-y QDs exhibit high PLQYs of 62.41% and dual-color emission with two strong emission peaks around at 400-450 nm and 600 nm,respectively.The enhanced peak at 400-450 nm is a result of the hybrid halides in CsPbBrxCl3-x host.Furthermore,the unique advantage of the optical emission and high PLQYs properties of the CsPbxMn1-xCl3 QDs has been demonstrated as invisible ink for encryption applications and polymer composites.Our gram-scale synthesis approach for Mn-doped all-inorganic perovskite QDs may boost the future research and practical applications of QDs-based white LED,spintronics,and molecular barcoding. | Lvming Dong Zhuo Chen Lei Ye Yan Yu Jianbing Zhang Huan Liu Jianfeng Zang | 2019 | Nano Research2019，12，7： | 6 |
| 7 | CEACAM6 promotes tumor migration, invasion, and metastasis in gastric cancer显示文摘(CEACAM6 ) Carcinoembryonic 抗原相关的房间粘附分子 6 证明在许多 humancancers 的增加的表示,和它的在表示上与提高的移植被联系,侵略,并且在 vivo 转移。这里,我们报导 CEACAM6 在房间衬里的胃的癌症(GC ) 和肿瘤纸巾是起来调整的。在 MKN-45 和 SGC-7901 GC 房间的 Over-expressionof CEACAM6 在 athymic 老鼠在 vitro 和转移支持了移植和侵略,而 MKN-28 和 SNU-16 GC 房间的移植和侵略被 knockdownof CEACAM6 压制。当 CEACAM6 是 over-expressedin SGC-7901 房间时,我们也观察到类固醇受体激活剂(C-SRC ) phosphorylation 被增加。一起拿,这些结果建议 CEACAM6 在 GC 作为 oncoprotein 工作并且可以是 importantmetastatic biomarker 和治疗学的目标。 | Yunqiang Zhang Mingde Zang Jianfang Li Jun Ji Jianian Zhang Xiaolei Liu Ying Qu Liping Su Chen Li Yinyan Yu Zhenggang Zhu Bingya Liu Min yan | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 5 |
| 8 | Anatomical variation of infra-pyloric artery origination: A prospective multicenter observational study (IPA-Origin)显示文摘Objective: Infra-pyloric artery(IPA) is an important anatomical landmark in treatment of gastric cancer and is the key vessel for pylorus-preserving gastrectomy and subgroup of infra-pyloric lymph nodes. However, its anatomical variation is not thoroughly understood. Our study aimed to clarify the origination of the IPA.Methods: We did this prospective, multicenter, open-label, observational study at gastric surgery departments of34 hospitals in China. Gastric cancer patients aged 18 years or older and scheduled to undergo elective total or distal gastrectomy were assigned. During the surgery, IPA dissecting and exposing the origination point with photographs or video clips were required. The primary outcome was the origination of the IPA. Analysis of variance, χ~2 tests and Fisher's tests were used to analyze the differences between groups. The study is registered at Clinicaltrials.gov(No. NCT03071237).Results: Between May 8 and July 31, 2017, 429 patients were assigned for the study, and 419(97.7%) patients had the IPA dissected and recorded through photograph or video and were included in the primary outcome analysis. The median age was 62 years old, and 73.7% were male. Among the patients, 78.5% received laparoscopic surgery. Single IPA origination was identified in 398(95.0%) patients, including gastroduodenal artery(GDA) in154(36.8%) patients, anterior superior pancreaticoduodenal artery(ASPDA) in 130(31.0%) patients, and right gastroepiploic artery(RGEA) in 114(27.2%) patients. Fifteen(3.6%) patients were identified with multiple IPA and 6(1.4%) patients were identified as IPA absence. The differences in the distribution of surgical approach(P=0.003) and geographic area(P=0.030) were statistically significant. No difference was shown in sex, age,gastrectomy type, tumor location, and clinical T, N and M stage.Conclusions: Our study found that the IPA originates from GDA, ASPDA and RGEA in similar proportions.Laparoscopic surgery may be more helpful in dissection of the IPA than open surgery. | Rulin Miao Jianjun Qu Zhengrong Li Daguang Wang Jiang Yu Weidong Zang Yong Li Fenglin Liu Jian Zhang Wu Song Kai Ye Su Yan Wei Wang Shuangyi Ren Lu Zang Changqing Jing Li Zhang Kuan Wang Weihua Fu Lin Fan Bin Liang Gang Zhao Jun Cai Li Yang Jiaming Zhu Jun You Kun Yang Qingxing Huang Zhaojian Niu Ning Ning Xingfeng Qiu Gang Ji Feng Liang Hua Huang Chao Gao Fei Shan Shuangxi Li Yongning Jia Lianhai Zhang Xiangji Ying Yan Zhang Zhaode Bu Xiangqian Su Gang Zhao Ziyu Li Jiafu Ji | 2018 | Chinese Journal of Cancer Research2018，30，5： | 5 |
| 9 | Engineering stem cell niches in bioreactors显示文摘Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body.Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as 'niches',to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix(synthetic and decellularized), paracrine/autocrine signaling and physical forces(i.e., mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general,bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering. | Meimei Liu Ning Liu Ru Zang Shang-Tian Yang Yan Li | 2013 | World Journal of Stem Cells2013，5，4： | 5 |
| 10 | Advances in neovascularization after diabetic ischemia显示文摘With the high incidence of diabetes around the world,ischemic complications cause a serious influence on people’s production and living.Neovascularization plays a significant role in its development.Therefore,neovascularization after diabetic ischemia has aroused attention and has become a hot spot in recent years.Neovascularization is divided into angiogenesis represented by atherosclerosis and arteriogenesis characterized by coronary collateral circulation.When mononuclear macrophages successively migrate to the ischemia anoxic zone after ischemia or hypoxia,they induce the secretion of cytokines,such as vascular endothelial growth factor and hypoxia-inducible factor,activate signaling pathways such as classic Wnt and phosphatidylinositol 3 kinase(PI3K)/protein kinase B(Akt)pathways,trigger oxidative stress response,activate endothelial progenitor cells or enter the glycolysis or lactic acid process and promote the formation of new blood vessels,remodeling them into mature blood vessels and restoring blood supply.However,the hypoglycemic condition has different impacts on neovascularization.Consequently,this review aimed to introduce the mechanisms of neovascularization after diabetic ischemia,increase our understanding of diabetic ischemic complications and their therapies and provide more treatment options for clinical practice and effectively relieve patients’pain.It is believed that in the near future,neovascularization will bring more benefits and hope to patients with diabetes. | Yue Cai Guang-Yao Zang Yan Huang Zhen Sun Li-Li Zhang Yong-Jiang Qian Wei Yuan Zhong-Qun Wang | 2022 | World Journal of Diabetes2022，13，11： | 4 |
| 11 | Simultaneous Prediction of Wheat Yield and Grain Protein Content Using Multitask Deep Learning from Time-Series Proximal Sensing显示文摘Wheat yield and grain protein content(GPC)are two main optimization targets for breeding and cultivation.Remote sensing provides nondestructive and early predictions of yield and GPC,respectively.However,whether it is possible to simultaneously predict yield and GPC in one model and the accuracy and influencing factors are still unclear.In this study,we made a systematic comparison of different deep learning models in terms of data fusion,time-series feature extraction,and multitask learning.The results showed that time-series data fusion significantly improved yield and GPC prediction accuracy with R 2 values of 0.817 and 0.809. | Zhuangzhuang Sun Qing Li Shichao Jin Yunlin Song Shan Xu Xiao Wang Jian Cai Qin Zhou Yan Ge Ruinan Zhang Jingrong Zang Dong Jiang | 2022 | Plant Phenomics2022，4，1： | 4 |
| 12 | A new phenylethanoid glycoside from Rabdosia lophanthoides(Buch.-Ham.ex D.Don) Hara.显示文摘新 phenylethanoid glycoside, 3-hydroxy-4-methoxy-β -phenylethoxy-O-α-l-rhamnopyranosyl-(1 →3 )-2-O-acetyl-O-β-d-glucopyranoside, 把 lophanthoside 称为 A,从 Rabdosia lophanthoides (Buch.-Ham.ex D.Don ) 被孤立 Hara。它的结构被分光镜的证据决定。 | Wei Sheng Feng Xin Yu Zang Xiao Ke Zheng Yan Zhi Wang | 2009 | Chinese Chemical Letters2009，20，4： | 4 |
| 13 | Analysis of period doubling bifurcation and chaos mirror of biped passive dynamic robot gait显示文摘With a reasonable parameter configuration,the passive dynamic walking model has a stable,efficient,natural periodic gait,which depends only on gravity and inertia when walking down a slight slope.In fact,there is a delicate balance in the energy conversion in the stable periodic gait,making the gait adjustable by changing the model parameters.Poincaré mapping is combined with Newton-Raphson iteration to obtain the numerical solution of the final state of the passive dynamic walking model.In addition,a simulation on the walking gait of the model is performed by increasing the slope step by step,thereby fixing the model's parameters synchronously.Then,the gait features obtained in the different slope stages are analyzed and discussed,the intrinsic laws are revealed in depth.The results indicate that the gait can present features of a single period,doubling period,the entrance of chaos,merging of sub-bands,and so on,because of the high sensitivity of the passive dynamic walking to the slope.From a global viewpoint,the gait becomes chaotic by way of period doubling bifurcation,with a self-similar Feigenbaum fractal structure in the process.At the entrance of chaos,the gait sequence comprises a Cantor set,and during the chaotic stage,sub-bands in the final-state diagram of the robot system present as a mirror of the period doubling bifurcation. | ZHAO Jie WU XiaoGuang ZANG XiZhe YAN JiHong | 2012 | Chinese Science Bulletin2012，57，14： | 4 |
| 14 | AMPK regulates anaphase central spindle Length by phosphorylation of KIF4A显示文摘激活安培的蛋白质 kinase (AMPK ) 是与基本生物过程联合细胞的精力状态的一个精力传感器。尽管内在的机制仍然保持大部分未知, AMPK 被认为与房间分割被连接。这里,我们证明 AMPK 机能上地在整个房间分割和那 AMPK 参与催化子单元,特别 2,顺序与分开的有丝分裂的仪器被联系。用量的 phosphoproteomics 分析,我们发现强壮的直接底层 KIF4A 是由在 Ser801 的 AMPK 的 phosphorylated。进一步的分析竞争地揭示了那 AMPK 和曙光 B phosphoregulates KIF4A 在有丝分裂的阶段期间由于重叠识别主题,为 KIF4A 依赖的中央锭子长度控制导致精致的 phosphoregulation。给 AMPK 的内在的察觉到精力的函数,我们的学习与细胞的葡萄糖应力连接中央锭子长度的 KIF4A 依赖的控制。 | Qian-Ru Li Xiu-Min Yan Lin Guo Jia Li Yi Zang | 2018 | Journal of Molecular Cell Biology2018，10，1： | 4 |
| 15 | Tandem Strip Mill's Multi-parameter Coupling Dynamic Modeling Based on the Thickness Control显示文摘The rolling process is determined by the interaction of a number of different movements,during which the relative movement occurs between the vibrating roll system and the rolled piece,and the roll system's vibration interacts with the strip's deformation and rigid movement.So many parameters being involved leads to a complex mechanism of this coupling effect.Through testing and analyzing the vibration signals of the mill in the rolling process,the rolling mill's coupled model is established with comprehensive consideration of the coupling interaction between the mill's vertical vibration,its torsional vibration and the working roll's horizontal vibration,and vibration characteristics of different forms of rolling mill's vibration are analyzed under the coupling effect.With comprehensive attention to the relationship between the roll system,the moving strip and the rolling parameters'dynamic properties,and also from the strip thickness control point of view,further research is done on the coupling mechanism between the roll system's movement and the moving strip's characteristics in the rolling process.As a result,the law of inertial coupling and the stiffness coupling effect caused by different forms of the roll system's vibration is determined and the existence of nonlinear characteristics caused by the elastic deformation of moving strip is also found.Furthermore,a multi-parameter coupling-dynamic model is established which takes the tandem strip mill as its research object by making a detailed kinematics analysis of the roll system and using the principle of virtual work.The coupling-dynamic model proposes the instruction to describe the roll system's movement,and analyzes its dynamic response and working stability,and provides a theoretical basis for the realization of the strip thickness'dynamic control. | PENG Yan ZHANG Yang SUN Jianliang ZANG Yong | 2015 | Chinese Journal of Mechanical Engineering2015，28，2： | 4 |
| 16 | Peniazaphilones A—I,Produced by Co-culturing of Mangrove Endophytic Fungi,Penicillium sclerotiorum THSH-4 and Penicillium sclerotiorum ZJHJJ-18显示文摘Co-culturing the mangrove endophytic fungus Penicillium sclerotiorum THSH-4 with Penicillium sclerotiorum ZJHJJ-18 in PDB medium resulted in the production of nine new azaphilones derivatives,peniazaphilones A—I(1-9),and six known azaphilones derivatives(10-15).Their structures were elucidated by analysis of detailed spectroscopic data,single-crystal X-ray diffraction,ECD spectra,^(13)C NMR calculation and DP4+analysis.It is noteworthy that compound 1 with an isoquinoline quinone moiety,was isolated from natural source for the first time.Besides,compound 2 is the third reported phenylhydrazone derivative from fungi.In the bioactivity assays,compounds 1,12,14 and 15(IC_(50)=4.71-17.64μmol/L)exhibited potent inhibition of LPS-induced NO release from RAW264.7 without obvious cytotoxicity within 50μmol/L compared to the positive control indomethacin(IC_(50)=35.27μmol/L).Moreover,compounds 1 and 13 displayed moderate to strong cytotoxicity to A549 and MDA-MB-435,with IC_(50) values in the range of 6.84-14.63μmol/L.Together compound 1 showed moderate broad-spectrum antibacterial activity. | Wencong Yang Jie Yuan Qi Tan Yan Chen Yujia Zhu Hongming Jiang Ge Zou Zhenming Zang Bo Wang Zhigang She | 2021 | Chinese Journal of Chemistry2021，39，12： | 3 |
| 17 | Air-Stable Ultrabright Inverted Organic Light-Emitting Devices with Metal Ion-Chelated Polymer Injection Layer显示文摘Here,this work presents an air-stable ultrabright inverted organic lightemitting device(OLED)by using zinc ionchelated polyethylenimine(PEI)as electron injection layer.The zinc chelation is demonstrated to increase the conductivity of the PEI by three orders of magnitude and passivate the polar amine groups.With these physicochemical properties,the inverted OLED shows a record-high external quantum efficiency of 10.0% at a high brightness of 45,610 cd m^(-2) and can deliver a maximum brightness of 121,865 cd m^(-2).Besides,the inverted OLED is also demonstrated to possess an excellent air stability(humidity,35%)with a half-brightness operating time of 541 h@1000 cd m^(-2) without any protection nor encapsulation. | Shihao Liu Chunxiu Zang Jiaming Zhang Shuang Tian Yan Wu Dong Shen Letian Zhang Wenfa Xie Chun-Sing Lee | 2022 | Nano-Micro Letters2022，14，1： | 3 |
| 18 | Ultrapure and highly efficient green light emitting devices based on ligand-modified CsPbBr3 quantum dots显示文摘All inorganic Cs PbBr3 perovskite quantum dots(QDs)have been recognized as promising optical materials tfabricate green light emission devices because of their excellent optical performance.However,regular Cs Pb BrQDs with an oleic acid(OA)ligand show poor stability,which limits their practical application.We replaced thOA ligand in Cs PbBr3 QDs with a 2-hexyldecanoic acid(DA)ligand and,in the synthesis,found that the nematerial has better optical properties than regular Cs PbBr3 QDs(Cs PbBr3-OA QDs).Due to the strong bindinenergy between the DA ligand and QDs,the ligand-modified Cs PbBr3 QDs(Cs PbBr3-DA QDs)show a higphotoluminescence quantum yield(PLQY)of 96%,while the PLQY of Cs PbBr3-OA QDs is 84%.Subsequentlythe Cs PbBr3 QDs coated on the blue light-emitting diode(LED)chips as green phosphors are demonstrated.Thcolor conversion from blue to pure green is achieved by adding the Cs PbBr3-OA QDs solution up to 60μL,whilthe pure green emission devices only need 18μL Cs PbBr3-DA QDs solution under the same concentration.Thultrapure,highly efficient green light-emitting devices based on Cs PbBr3-DA QDs exhibit a luminous efficiencof 43.6 lm/W with a CIE(0.2086,0.7635)under a 15.3 m A driving current.In addition,the green emissiowavelength of the devices based on Cs PbBr3-DA QDs almost has no shift,even under a high injection currenThese results highlight the promise of DA ligand-modified Cs PbBr3 QDs for light-emitting devices and enrich thapplication field of ligand-modified Cs PbBr3 QDs. | Dongdong Yan Shuangyi Zhao Huaxin Wang Zhigang Zang | 2020 | Photonics Research2020，8，7： | 3 |
| 19 | Integration of enzyme immobilization and biomimetic catalysis in hierarchically porous metal-organic frameworks for multi-enzymatic cascade reactions显示文摘Multiple enzymes-induced biological cascade catalysis is indispensable in biotechnology and industrial processes. Nevertheless,the drawbacks of most natural enzymes, including poor stability and recyclability and sensitivity to the environment, have hindered their broader application. Here, we report a facile strategy to prepare a biomimetic cascade reaction system by combining the advantages of enzyme immobilization and biomimetic catalysis in a one-pot reaction system based on the hierarchically porous metal-organic frameworks(HP-MOFs). The hierarchically porous zirconium-porphyrin-based MOF(HPPCN-222(Fe)) synthesized by modulator-induced strategy possessed tunable hierarchical porous and peroxidase-like activity,permitting them to act as not only an efficient immobilization matrix for glucose oxidase(GOx) but also peroxidase mimics to catalyze the cascade for glucose detection. A stable, anti-interference and reusable colorimetric biosensor for glucose detection was successfully established through GOx@HP-PCN-222(Fe) on the basis of the artificial tandem catalysis. Moreover, the GOx@HP-PCN-222(Fe)-fabricated electrode was available for glucose detection by electrochemical method. This work provides a potentially universal method to design functional multi-enzymatic cascade reaction systems by integrating the merits of enzyme encapsulation and biomimetic catalysis in HP-MOFs. | Shu-Fan Li Yan Chen Yi-Shu Wang Hui-Lin Mo Shuang-Quan Zang | 2022 | Science China Chemistry2022，65，6： | 3 |
| 20 | Gut microbiota mediates the absorption of FLZ,a new drug for Parkinson’s disease treatment显示文摘The gut microbiota plays an important role in regulating the pharmacokinetics and pharmacodynamics of many drugs.FLZ,a novel squamosamide derivative,has been shown to have neuroprotective effects on experimental Parkinson’s disease(PD)models.FLZ is under phase I clinical trial now,while the underlying mechanisms contributing to the absorption of FLZ are still not fully elucidated.Due to the main metabolite of FLZ was abundant in feces but rare in urine and bile of mice,we focused on the gut microbiota to address how FLZ was metabolized and absorbed.In vitro studies revealed that FLZ could be exclusively metabolized to its major metabolite M1 by the lanosterol 14 alpha-demethylase(CYP51)in the gut microbiota,but was almost not metabolized by any other metabolism-related organs,such as liver,kidney,and small intestine.M1 was quickly absorbed into the blood and then remethylated to FLZ by catechol O-methyltransferase(COMT).Notably,dysbacteriosis reduced the therapeutic efficacy of FLZ on the PD mouse model by inhibiting its absorption.The results show that the gut microbiota mediate the absorption of FLZ through a FLZ-M1-FLZ circulation.Our research elucidates the vital role of the gut microbiota in the absorption of FLZ and provides a theoretical basis for clinical pharmacokinetic studies and clinical application of FLZ in the treatment of PD. | Junmei Shang Shurong Ma Caixia Zang Xiuqi Bao Yan Wang Dan Zhang | 2021 | Acta Pharmaceutica Sinica B2021，11，5： | 3 |
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