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| 1 | Get effective polyclonal antisera in one month显示文摘According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells. | YUAN XIN HU, Ju YUAN QUO, Lu SHEN, YAN CHEN, Zu CHUAN ZHANG, YONG LIAN ZHANGState Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, | 2002 | Cell Research2002，12，2： | 45 |
| 2 | Outbreak of Dengue Fever in Central China,2013显示文摘In 2013,the first dengue fever(DF) outbreak in central China was reported in the central of Henan province,northern temperate regions,although they have been sequentially recorded in Southern China.106 suspected DF cases were reported and 73 patients were confirmed dengue virus type 3(DEN-3) infections.62/392(15.8%) local health persons showed DEN antibodies positive.To this day Henan is the northernmost province in China which has been reported about outbreak of DF and what is important is that it warns us the endemic range of DF has been expanded geographically in China. | HUANG Xue Yong MA Hong Xia WANG Hai Feng DU Yan Hua SU Jia LI Xing Le TANG Xiao Yan MA Hong Peng ZU Bing Can ZHANG Qiao Hong CHEN Hao Min XU Bian Li | 2014 | Biomedical and Environmental Sciences2014，27，11： | 30 |
| 3 | Pairing of integrins with ECM proteins determines migrasome formation显示文摘 | Danni Wu Yue Xu Tianlun Ding Yan Zu Chun Yang Li Yu | 2017 | Cell Research2017，27，11： | 14 |
| 4 | Numerical Simulation of Electroosmotic Flow near Earthworm Surface显示文摘The electroosmotic flow near an earthworm surface is simulated numerically to further understand the anti soil adhesion mechanism of earthworm. A lattice Poisson method is employed to solve electric potential and charge distributions in the electric double layer along the earthworm surface. The external electric field is obtained by solving a Laplace equation. The electroosmotic flow controlled by the Navier-Stokes equations with external body force is simulated by the lattice Boltzmann method. A benchmark test shows that accurate electric potential distributions can be obtained by the LPM. The simulation shows that the moving vortices, which probably contribute to anti soil adhesion, are formed near earthworm body surface by the nonuniform and variational electrical force. | Y.Q. Zu Y.Y. Yan | 2006 | Journal of Bionic Engineering2006，3，4： | 12 |
| 5 | Study on the Expression Levels of CXCR4, CXCL12, CD44, and CD147 and Their Potential Correlation with Invasive Behaviors of Pituitary Adenomas显示文摘Objective To evaluate the factors of CXCR4, CXCL12, CD44, and CD147 as early potential diagnostic biomarkers by determining their expression levels in invasive and non-invasive pituitary adenomas. Methods Fresh pituitary adenoma specimens were collected from 35 pituitary adenoma (21 invasive and 14 non-invasive) patients who underwent surgical treatment in our Neurosurgery Department between January and April of 2009. The expression levels of CXCR4, CXCL12, CD44, and CD147 were evaluated firstly by flow cytometry, fluorescence microscopy in single cell suspensions, and then by immunohistochemical staining of paraffin tissue sections. Results Flow cytometric analyses showed that the percentage of CXCR4-and CXCL12-positive cells from invasive pituitary adenomas (IPA) was significantly higher in the single cell suspensions than that from non-invasive pituitary adenomas (nIPA) (P<0.05). Immunohistochemical staining revealed that CXCR4 and CXCL12 staining index scores of the invasive pituitary adenomas were significantly higher than those of the non-invasive pituitary adenomas (P<0.05). In contrast, neither flow cytometry nor immunohistochemical staining demonstrated significant difference between CD44 and CD147 expression levels, respectively. Conclusion Expression levels of CXCR4 and CXCL12 are correlated with the invasiveness of pituitary adenomas. Therefore, rather than CD44 and CD147, CXCR4 and CXCL12 may potentially serve as biomarkers for early detection of pituitary adenomas. | XING Bing KONG Yan Guo YAO Yong LIAN Wei WANG Ren Zhi REN Zu Yuan | 2013 | Biomedical and Environmental Sciences2013，26，7： | 9 |
| 6 | Contrast-enhanced ultrasound for quantitative assessment of portal pressure in canine liver fibrosis显示文摘AIM: To explore the feasibility of non-invasive quantitative estimation of portal venous pressure by contrast-enhanced ultrasound(CEUS) in a canine model.METHODS: Liver fibrosis was established in adult canines(Beagles; n = 14) by subcutaneous injection of carbon tetrachloride(CCl4). CEUS parameters, including the area under the time-intensity curve and intensity at portal/arterial phases(Qp/Qa and Ip/Ia, respectively), were used to quantitatively assess the blood flow ratio of the portal vein/hepatic artery at multiple time points. The free portal venous pressures(FPP) were measured by a multi-channel baroreceptor using a percutaneous approach at baseline and 8, 16, and 24 wk after CCl4 injections in each canine. Liver biopsies were obtained at the end of 8, 16, and 24 wk from each animal, and the stage of the fibrosis was assessed according to the Metavir scoring system. A Pearson correlation test was performed to compare the FPP with Q p/Q a and I p/I a.RESULTS: Pathologic examination of 42 biopsies from the 14 canines at weeks 8, 16, and 24 revealed that liver fibrosis was induced by CCl4 and represented various stages of liver fibrosis, including F0(n = 3), F1(n = 12), F2(n = 14), F3(n = 11), and F4(n = 2). There were significant differences in the measurements of Qp/Qa(19.85 ± 3.30 vs 10.43 ± 1.21, 9.63 ± 1.03, and 8.77 ± 0.96) and Ip/Ia(1.77 ± 0.37 vs 1.03 ± 0.12, 0.83 ± 0.10, and 0.69 ± 0.13) between control and canine fibrosis at 8, 16, and 24 wk, respectively(all P < 0.001). There were statistically significant negative correlations between FPP and Q p/Q a(r =-0.707, P < 0.001), and between FPP and Ip/Ia(r =-0.759, P < 0.001) in the canine fibrosis model. Prediction of elevated FPP based on Q p/Q a and I p/I a was highly sensitive, as assessed by the area under the receiveroperating curve(0.866 and 0.895, respectively).CONCLUSION: C E U S i s a p o t e n t i a l m e t h o d t o accurately, but non-invasively, estimate portal venous pressure through measurement of Qp/Qa and Ip/Ia parameters. | Lin Zhai Lan-Yan Qiu Yuan Zu Yan Yan Xiao-Zhuan Ren Jun-Feng Zhao Yu-Jiang Liu Ji-Bin Liu Lin-Xue Qian | 2015 | World Journal of Gastroenterology2015，21，15： | 3 |
| 7 | Clinicopathologic characteristics and therapeutic responses of Chinese patients with non-small cell lung cancer who harbor an anaplastic lymphoma kinase rearrangement显示文摘Introduction: The rearrangement of the anaplastic lymphoma kinase(ALK) gene accounts for approximately 1%–6% of lung adenocarcinoma cases and deines a molecular subgroup of tumors characterized by clinical sensitivity to ALK inhibitors such as crizotinib. This study aimed to identify the relationship between ALK rearrangement and the clinico?pathologic characteristics of non?small cell lung cancer(NSCLC) and to analyze the therapeutic responses of crizotinib and conventional chemotherapy to ALK rearrangement in NSCLC patients.Methods: A total of 487 lung cancer patients who underwent testing for ALK rearrangement in our department were included in this study. ALK rearrangement was examined by using fluorescence in situ hybridization(FISH) assay.Results: Among the 487 patients, 44(9.0%) were diagnosed with ALK rearrangement by using FISH assay. In 123 patients with adenocarcinoma who were non?smokers and of a young age(≤58 years old), the frequency of ALK rearrangement was 20.3%(25/123). Short overall survival(OS) was associated with non?adenocarcinoma tumor type(P = 0.006), poorly diferentiated tumors(P al growth factor rece= 0.001), advanced?stage tumors(P < 0.001), smoking history(P ptor(EGFR)(P = 0.008), and wild?type epidermrter time to cancer p= 0.008). Moreover, patients with poorly diferentiated and advanced?stage tumors had a shorogression compared with those with well diferentiated(P = 0.023) and early?stage tumors(P = 0.001), respectively.Conclusions: ALK?rearranged NSCLC tends to occur in younger individuals who are either non?smokers or light smokers with adenocarcinoma. Patients with ALK rearrangement might beneit from ALK inhibitor therapy. | Sha Fu Hai‑Yun Wang Fang Wang Ma‑Yan Huang Ling Deng Xiao Zhang Zu‑Lu Ye Jian‑Yong Shao | 2015 | Chinese Journal of Cancer2015，34，9： | 3 |
| 8 | Hercynian-Indosinian Sedimento-Tectonic Evolution of the Nanpanjiang Basin显示文摘Following the orogeny in the Early Palaeozoic, the tectonic evolution of the Nanpanjiang basin in the Hercynian-Indosinian stage constituted a complete Wilson Cycle, made up of the stages of early rifting (D-P1), late rifting and passive margin developement (P2-T1), and peripheral foreland basin developement (T2-T3). The REE data of the initial Early Triassic basalts from the southern part of the basin has verified the existence of the oceanic crust once in the basinal evolution. The basin developed into a foreland one in the Middle-Late Triassic, indicating that the Yangtze plate once collided with the Indo-Sinian plate along the Black River fracture zone. | Qin Jianhua, Wu Yinglin, Yan Yangji and Zu ZhongfaResearch Center of Sedimentary and Energy Geology, Chinese Academy of Geological Sciences, Chengdu, Sichuan | 1996 | Acta Geologica Sinica(English Edition)1996，70，4： | 3 |
| 9 | Study on the Structure of Supramolecular Inclusion Complex of β-Cyclodextrin with Retinoic Acid显示文摘Inclusion compound of retinoic acid with (-cyclodextrin was prepared by coprecipitating method, the structure of resulting product was studied by elemental analysis, differential scanning caloriemetry(DSC) analysis, FT-IR spectroscopy and X-ray diffractometry, and the formed supramolecule self-assembles in aqueous solution according to molar ratio 2:1 of host-guest. | Yan Ling ZHANG1, Wei Sheng LIU1, Yang LI1, Xue Yi MA1*, Yao Zu CHEN2 1National Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou 730000 2Department of Chemistry, Zhejiang University, Hangzhou 310027 | 2001 | Chinese Chemical Letters2001，12，4： | 3 |
| 10 | Wetting transition energy curves for a droplet on a square-post patterned surface显示文摘Due to the property of water repellence, biomimetic superhydrophobic surfaces have been widely applied to green technologies, in turn inducing wider and deeper investigations on superhydrophobic surfaces. Theoretical, experimental and numerical studies on wetting transitions have been carried out by researchers, but the mechanism of wetting transitions between Cassie-Baxter state and Wenzel state,which is crucial to develop a stable superhydrophobic surface, is still not fully understood. In this paper,the free energy curves based on the transition processes are presented and discussed in detail. The existence of energy barriers with or without consideration of the gravity effect, and the irreversibility of wetting transition are discussed based on the presented energy curves. The energy curves show that different routes of the Cassie-to-Wenzel transition and the reverse transition are the main reason for the irreversibility. Numerical simulations are implemented via a phase field lattice Boltzmann method of large density ratio, and the simulation results show good consistency with the theoretical analysis. | Wei Gong Yingqing Zu Sheng Chen Yuying Yan | 2017 | Science Bulletin2017，62，2： | 3 |
| 11 | Synthesis and Antitumor Activity of4β-(1,2,3-Triazol-1-yl) podophyllotoxins显示文摘Three 4β-(1,2,3-triazol-1-yl) podophyllotoxins 2~4 have been synthesized and testedtar their antitumor activity in vitro. The compound 3 was found to be much more cytotoxic than theclinically used etoposide (VP-16) against L 1210 cells. | Chang Nian CAO~2 Yu Yin CHEN1 Yan Guang WANG1 Yao Zu CHEN(1Department of Chemistry, Zhejiang University, Hangzhou 310027)(2Department of Applied Chemistry, Qinghai University, Xining 810000) | 1999 | Chinese Chemical Letters1999，10，12： | 3 |
| 12 | Wind tunnel simulation experiment and investigation on the electrification of sandstorms显示文摘Electric discharge phenomenon is often observed during the passage of sandstorms over desert regions and sometimes does great damage to human beings. Based on previous researches on the electrification of sandstorms by the scholars both at home and aboard,the authors conducted the simulation experiments on the electric fields of sandstorm-triggered creeping-saltating sand, suspending sand and watered suspending sand in the large-scale wind tunnel. According to the experimental results the electric field structure and its formation mechanism were analysed and discussed, the quantity of electric charges and electric field strengthwere measured and calculated, and finally several conclusions were drawn. | QU Jianjun YAN Muhong DONG Guangrong ZHANG Hongfa ZU Ruiping TUO Wanquan ZHAO Aiguo XIAO Zhenghua LI Fang YANG Bao | 2004 | Science China Earth Sciences2004，47，6： | 2 |
| 13 | Some Bonnesen-style Inequalities for Higher Dimensions显示文摘We discuss the higher dimensional Bonnesen-style inequalities.Though there are many Bonnesen-style inequalities for domains in the Euclidean plane R2 few results for general domain in R n(n ≥ 3) are known.The results obtained in this paper are for general domains,convex or non-convex,in Rn. | Jia Zu ZHOU Yan Hua DU Fei CHENG | 2012 | Acta Mathematica Sinica,English Series2012，28，12： | 2 |
| 14 | Factors Affecting Patient Satisfaction with Community Health Service under the Gatekeeper System:A Cross-sectional Study in Nanjing,China显示文摘The gatekeeper policy has been implemented for approximately ten years on a pilot population in China. It is necessary to assess the satisfaction of patients utilizing community health service(CHS) under the gatekeeper system. Our study showed that the cognition of gatekeeper policy was associated with four dimensions including doctor‐patient relationships, information and support, organization of care, and accessibility(P < 0.001). One or more factors such as gender and self‐perceived health scores also affected their satisfaction. General practitioners must be prepared to focus on these aspects of information and support, organization of care, and accessibility as indicators of potential opportunities for improvement. Additionally, policymakers can improve patients' satisfaction with CHS by strengthening their awareness of the gatekeeper policy. | LI Wen Zhen GAN Yong ZHOU Yan Feng CHEN Ya Wen LI Jing KKANDAWIRE Naomiem HU Sai QIAO Yan LU Zu Xun | 2017 | Biomedical and Environmental Sciences2017，30，9： | 2 |
| 15 | THEMIS statistical study on the plasma properties of high-speed flows in Earth's magnetotail显示文摘Using Time History of Events and Macroscale Interactions during Substorms(THEMIS) observations from 2007 to 2011 tail seasons, we study the plasma properties of high speed flows(HSFs) and background plasma sheet events(BPSs) in Earth's magnetotail(|Y_(GSM)|<13R_E, |Z_(GSM)|<5R_E, –30R_E | PAN Dong Xiao SUN Wei Jie SHI Quan Qi TIAN An Min YAO Zhong Hua FU Sui Yan ZONG Qiu Gang ZHOU Xu Zhi PU Zu Yin | 2016 | Science China Earth Sciences2016，59，3： | 2 |
| 16 | 用Lattice Boltzmann方法模拟1/4圆腔内的定常层流运动显示文摘分析格子Boltzmann方法中二阶精度的曲线边界处理方法.应用格子Boltzmann方法及其边界处理方法模拟1/4圆腔内的定常层流运动,引入流线图和等涡线图分析流场随如数的变化.并且发现当Re数在10~100区间内变化时,随着Re数的增大,顺时针旋转流场的涡心位置偏离X轴的角度逐渐减小,而逆时针旋转流场的涡心位置偏离X轴的角度却越来越大. | 施卫平 Zu Yingqing Yan Yuying | 2008 | 计算物理2008，25，1： | 2 |
| 17 | Stability of superconducting Nd_(0.8)Sr_(0.2)NiO_(2) thin films显示文摘The discovery of superconducting states in the nickelate thin film with infinite-layer structure has paved a new way for studying unconventional superconductivity.So far,research in this field is still very limited due to difficulties in sample preparation.Here we report the successful preparation of the superconducting state of Nd_(0.8)Sr_(0.2)NiO_(2)thin film(T_(c)=8.0-11.1 K)and study the stability of such films in the ambient environment,water,and under electrochemical conditions.Our work demonstrates that the superconducting state of Nd_(0.8)Sr_(0.2)NiO_(2)is remarkably stable,which can last for at least 47-day continuous exposure to air at 20°C and 35%relative humidity.We also show that the superconductivity disappears after being immersed in de-ionized water at room temperature for 5 h.Surprisingly,it can also survive under ionic liquid gating conditions with an applied voltage of about 4 V,which is even more stable than conventional perovskite complex oxides. | Xiang Ding Shengchun Shen Huaqian Leng Minghui Xu Yan Zhao Junrui Zhao Xuelei Sui Xiaoqiang Wu Haiyan Xiao Xiaotao Zu Bing Huang Huiqian Luo Pu Yu Liang Qiao | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，6： | 2 |
| 18 | Two New Sesquiterpenes from Celastrus Paniculatus.Subsp.Paniculatus显示文摘Two new β-dihydroagarofuran sesquiterpene polyol esters were isolated from Celastrus paniculatus, subsp. paniculatus.Their Structures were deduced on the basis of spectral analyses including 2D NMR. CO2 supercritical fluid was used in separation. | Kun ZHANG Yan Hong WANG Yao Zu CHEN (The State Key Laboratory of Applied Organic Chemistry, Lanzhou University,Lanzhou. 730000)(Institute of Organic Chemistry, Zhejiang University, Hangzhou,310027)(Guangdong University of Technology,Guangzhau,510090) | 1997 | Chinese Chemical Letters1997，8，11： | 2 |
| 19 | Role and mechanism of radiological protection cream in treating radiation dermatitis in rats显示文摘OBJECTIVE: To explore the role and mechanism of a radiation protection cream(Rp) in the treatment of radiation dermatitis, and to accumulate necessary technical information for a new drug report on Rp.METHODS: High-performance liquid chromatography was used to establish the method of measuring the main effective ingredients of sovereign and adjuvant herbs of Rp drugs, and to formulate the draft quality standards of Rp. A total of 48Sprague-Dawley male rats were randomly divided into the Model,Trolamine cream(Tc), Rp and Blank groups according to a random number table method.The skin of each rat's buttocks was irradiated using an electron linear accelerator to establish an acute radiation dermatitis model. The histological changes were observed under light microscopy and electron microscopy during wound healing and the effect of Rp on rat fibroblast Ku70/80 gene expression was detected at the transcriptional level.RESULTS: Pathological examination revealed that Rp protected the cellular and subcellular structures of skin after irradiation, promoting the proliferation and restoration of collagen fibers. Ku70/80 mRNA expression levels in the Rp and Tc groups were higher than that in the model group(P<0.05).Moreover,The majority of grade radiation dermatitis relative to the Model, Rp and Tc groups for reducing grade Ⅲ and Ⅳ dermatitis efficiency were 85.7%and 69.2%(P<0.05), respectively.The efficacy of Rp group in treating radiation dermatitis was better than the Trolamine cream group by16.5%(P<0.05).CONCLUSION: Compared with Tc, Rp had certain advantages in the efficacy and performance to price ratio.Thus, Rp is considered an effective alternative formulation for the prevention and treatment of radiation dermatitis. | Guohong Zu Yan Dou Qingfen Tian Houwei Wang Weichong Zhao Fusheng Li | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 2 |
| 20 | Numerical simulation of bubbles deformation, flow, and coalescence in a microchannel under pseudo-nucleation conditions显示文摘 | YAN Y ZU Y | 2011 | Heat Transfer Engineering2011，32，1314： | 1 |
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