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| 1 | Inhibitory effect of topiramate on Lewis lung carcinoma metastasis and its relation with AQP1 water channel显示文摘AIM: To study the effect of topiramate on tumor metastasis and its relation with aquaporin 1 (AQP1) water channel. METHODS: Lewis lung carcinoma metastatic model was used to determine the effect of topiramate on tumor growth and metastasis. Colorimetric estimation was used to investigate the action of topiramate on carbonic anhydrase (CA) activity. Western blotting and immunohistochemical analysis were used to study the influence of topiramate on AQP1 water channel expression in lungs or tumor tissues of mice bearing Lewis lung carcinoma.RESULTS: Treatment with topiramate (120 mg.kg^-1.d^-1, ig for 20 d) reduced the growth of primary tumor signifi-cantly (P<0.05). Its inhibitory rate of metastasis was 81.25 %. Topiramate inhibited CA activity in lungs of mice in a dose-dependent manner. Topiramate apparently decreased AQP1 protein expression and immunostaining in lungs or in tumor microvessel endothelial cells of mice. CONCLUSION: Suppression of AQP1 water channel expression may be an important pathway for the inhibitory effect of topiramate on tumor metastasis. | BingMA YangXIANG TaoLI He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，1： | 25 |
| 2 | Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis显示文摘AIM: To study effects of acetazolamide on aquaporin-1 (AQP1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQP1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. RESULTS: Immunohistochemical localization of AQP1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSION: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis. | YangXIANG BingMA TaoLI Jun-weiGAO He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，6： | 23 |
| 3 | Cloning,Characterization and Primary Function Study of a Novel Gene,Cymg1,Related to Family 2 Cystatins显示文摘Cystatins are cysteine proteinase inhibitors. We found two expression sequence tags (ESTs), CA463109 and AV042522, from a mouse testis library using Digital differential display (DDD). By electrical hybridization, a novel gene, Cymg1 (GenBank accession No. AY600990), which has a full length of 0.78kb, and contains four exons and three introns, was cloned from a mouse testis cDNA library. The gene is located in the 2G3 area of chromosome 2. The full cDNA encompasses the entire open reading frame, encoding 141 amino acid residues. The protein has a cysteine protease inhibitor domain that is related to the family 2 cystatins but lacks critical consensus sites important for cysteine protease inhibition. These characteristics are seen in the CRES subfamily, which are related to the family 2 cystatins and are expressed specifically in the male reproductive tract. CYMG1 has a 44% (48/108) identity with mouse CRES and 30% (42/140) identity with mouse cystatin C. Northern blot analysis showed that the Cymg1 is specifically expressed in adult mouse testes. Cell location studies showed that the GFP-tagged CYMG1 protein was localized in the cytoplasm of HeLa cells. Immunohistochemistry revealed that the CYMG1 protein was expressed in mouse testes spermatogonium, spermatocytes, round spermatids, elongating spermatids and spermatozoa. RT-PCR results also showed that Cymg1 was expressed in mouse testes and spermatogonium. The Cymg1 expression level varied in different developmental stages: it was low 1 week postpartum, steadily increased 2 to 5 weeks postpartum, and was highest 7 weeks postpartum. The expression level at 5 weeks postpartum was maintained during 13 to 57 weeks postpartum. The Cymg1 expression level in the testes over different developmental stages correlates with the mouse spermatogenesis and sexual maturation process. All these indicate that Cymg1 might play an important role in mouse spermatogenesis and sexual maturation. | YangXIANG Dong-SongNIE JianWANG Xiao-JunTAN YunDENG Shu-WeiLUO Guang-XiuLU | 2005 | Acta Biochimica et Biophysica Sinica2005，37，1： | 8 |
| 4 | Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocyte显示文摘AIM: To study the effects of acetazolamide and anordiol on osmotic water permeability in aquaporin 1 (AQP1)-cRNA injected Xenopus oocyte and their mechanisms. METHODS: AQP1 gene constructed in pBluescript was transcripted into cRNA in vitro and then the cRNA was injected in Xenopus oocytes. The effects of acetazolamide and anordiol on the water transport function of AQP1 were observed by assaying the osmotic swelling of oocytes.In addition, their effects on protein expression of AQP1 were quantitatively investigated by Western blotting method.RESULTS: After incubation for 15 min or 72 h, acetazolamide, a carbonic anhydrase inhibitor, equally reduced the water permeability of AQPI-cRNA injected oocyte in a dose-dependent manner. After incubation for 72 h, anordiol,an antiestrogen with partial estrogenic activity, reduced the osmotic water permeability dose dependently as well;however, no discernable action was observed after incubation with anordiol for 15 min. The Western blotting analysis showed that acetazolamide did not influence the protein expression of AQP1. However, after incubation for 72 h with anordiol (10 μmol/L), the quantity of AQP1 in the oocyte membrane was decreased dramatically(P<0.05). CONCLUSION: Both acetazolamide and anordiol inhibited the osmotic water permeability of AQP1-cRNA injected oocyte, but their mechanisms were different. Acetazolamide functionally inhibited the osmotic water permeability of AQP1, whereas anordiol primarily decreased the amount of AQP1 protein in the oocyte membrane. | BingMA YangXIANG Sheng-meiMU TaoLI He-mingYU Xue-junLI | 2004 | Acta Pharmacologica Sinica2004，25，1： | 6 |
| 5 | Molecular Cloning of a Novel Mouse Testis-specific Spermatogenic Cell Apoptosis Inhibitor Gene mTSARG7 as a Candidate Oncogene显示文摘A novel mouse gene, mTSARG7 (GenBank accession No. AY489184), with a full cDNA length of 2279 bp and containing 12 exons and.ll introns, was cloned from a mouse expressed sequence tag (GenBank accession No. BE644543) that was significantly up-regulated in cryptorchidism. The gene was located in mouse chromosome 8A1.3 and encoded a protein containing 403 amino acid residues that was a new member of the acyltransferase family because the sequence contained the highly conserved phosphate acyltransferase (PlsC) domain existing in all acyltransferase-like proteins. The mTSARG7 protein and AU041707 protein shared 83.9% identity in 402 amino acid residues. Expression of the mTSARG7 gene was restricted to the mouse testis. The results of the in situ hybridization analysis revealed that the mTSARG7 mRNA was expressed in mouse spermatogonia and spermatocytes. Subcellular localization studies showed that the EGFPtagged mTSARG7 protein was localized in the cytoplasm of GC-1 spg cells. The mTSARG7 mRNA expression was initiated in the mouse testis in the second week after birth, and the expression level increased steadily with spermatogenesis and sexual maturation of the mouse. The results of the heat stress experiment showed that the mTSARG7 mRNA expression gradually decreased as the heating duration increased. The pcDNA3.1 Hygro(-)/mTSARG7 plasmid was constructed and introduced into GC- 1 spg cells by liposome transfection. The mTSARG7 can accelerate GC-1 spg cells, causing them to traverse the S-phase and enter the G2-phase, compared with the control group where this did not occur as there was no transfection of mTSARG7. In conclusion, our results suggest that this gene may play an important role in spermatogenesis and the development of cryptorchid testes, and is a testis-specific apoptosis candidate oncogene. | Xiao-JunTAN Xiao-WeiXING Lu-YunLI Zhao-DiWU Chang-GaoZHONG Dong-SongNIE Jun-JiangFU YangXIANG YunDENG Guang-XiuLU | 2005 | Acta Biochimica et Biophysica Sinica2005，37，6： | 3 |
| 6 | Effects of pump source on spectra of optically pumped sub- millimeter wave laser 显示文摘 | Bao Yangxiang Huang Xiao He Zhihong | 2006 | International Journal of Infrared and Millimeter Waves2006，27，： | 1 |
| 7 | Cloud security defence to protect cloud computing against HTTP - DoS and XML - DoS attacks显示文摘 | Ashley Chonka YangXiang n WanleiZhou AlessioBonti | | 0，，34： | 1 |
| 8 | Microstructure and mechanical properties of austempered high silion cast steel 显示文摘 | Yangxiang Li Xiang Chen | 2001 | Materials Science and Engineering2001，308，2： | 1 |
| 9 | Microstructure and mechanical properties of austempered high silion cast steel显示文摘 | Yangxiang Li Xiang Chen | 2001 | Materials Science and Engineering2001，，： | 1 |
| 10 | A last and robust imagesegmentation using FCM with spatial information显示文摘 | Wang Yangxiang Bu Juan | 2009 | Digital Signal Processing2009，11，7： | 1 |
| 11 | Reread “Blanc de Chine”显示文摘As Chinese culture is rich in cultural connotations, Dehua White Porcelain became outstanding Chinese porcelain with monochrome glazes in the ceramic history. “Blanc de Chine” was once glorious along the Maritime Silk Road. Both artistic creation and cultural transmission need splendid expression taking root in national tradition and keeping a foothold in the spirit of the time. | Hou Yangxiang | 2017 | China & The World Cultural Exchange2017，83，9： | 0 |
| 12 | Proteomics: a new approach for drug discovery显示文摘Proteomics is a bridge that crosses genome to drug discovery. Proteomic studies will provide possible targets for therapeutic usage and moreover increase the efficiency of the downstream of drug discovery process. By using the 2-D electrophoresis combining with MS technology, which is most preva-lent techniques of proteome, we have identified more than | Xue-junLI YangXIANG Hui-kaiTIAN BingMA Xiao-haoYAO Hong-meiZHANG He-mingYU | 2004 | Acta Pharmacologica Sinica2004，25，11： | 0 |
| 13 | IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA显示文摘Objective To evaluate the expression and function activity of P-glycoprotein (P-gp) in human mononuclear cells (MNCs) in vitro transfected by multidrug resistance-1(MDR1) mRNA. Methods Two MDR1 cDNA vectors, pT7TS_MDR1 and pGEM5Zf(+)_MDR1, were constructed and transcripted in vitro. Vector pGEM5Zf(+)_MDR1 only contained the coding region of mdr1 cDNA, and pT7TS_MDR1 also included Xeponus β-globin 5’ and 3’ untranslated region. MNCs were prepared from peripheral blood of parvicellular lung cancer patient. The two human mdr1 mRNAs were then transferred into human MNCs in vitro by DOTAP. And the expression efficiency and pump function of P-gp were measured with flow cytometry. Results Expression of P-gp significantly elevated in both transferred cells compared with untransferred cells (P < 0.01). And pT7TS_MDR1 showed higher capability in elevating the expression of P-gp than pGEM5Zf(+)_MDR1 (P < 0.01). The P-gp function was elevated in both pT7TS_MDR1 and pGEM5Zf(+)_MDR1 groups. The survival ratio of MNCs in erythrocyte-lysis-solution (ELS, 86.07%) and lymphocyte-isolation-solution (LIS, 83.67%) had no significant difference. The CD34+ cells content of the MNCs used for transfection was 2.65% and 1.01% in ELS and LIS group, respectively (P < 0.01).Conclusions It is a feasible approach to improve P-gp expression in human MNCs by transfection of MDR-1 mRNA. And the ELS may be more suitable for purifing MNCs for mRNA transfection than LIS. | YangXiang LeiLi FangTian Xiu-yuYang | 2005 | Chinese Medical Sciences Journal2005，20，1： | 0 |
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