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| 1 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 2 | Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses显示文摘AIM: To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigenspecific T cell responses or not. METHODS: DCs were generated from peripheral blood mononuclear cells of patients with chronic hepatitis B (CHB) infection and healthy donors. We compared the phenotypes of these DCs and their ability to secrete cytokines and to participate in mixed lymphocyte reactions. In addition, autologous lymphocytes were cultured with DCs loaded with HBV core region peptide HBcAg8-27, an epitope recognized by cytotoxic T lymphocytes(CTL), and bearing human leucocyte antigen (HLA)-A2 for 10 d. Cytokine secretion and lytic activity against peptide-pulsed target cells were assessed. RESULTS: DCs with typical morphology were generated successfully by culturing peripheral blood mononuclear cells (PBMCs) from CriB patients with AIM-V containing GM-CSF and IL-4. Compared with DCs from normal donors, the level of CD80 expressed in DCs from CHB patients was lower, and DCs from patients had lower capacity of stimulate T cell proliferation. When PBMCs isolated from patients with chronic or acute hepatitis B infection and from normal donors were cocultured with HBcAg18-27 peptide, the antigen-specific memory response of PBMCs from acute hepatitis B patients was stronger than that of PBMCs from chronic hepatitis B patients or normal donors. PBMCs cocultured with DCs treated with HBcAg18-27 CTL epitope peptide induced an antigen-specific T cell reaction, in which the level of secreted cytokines and lyric activity were higher than those produced by memory T cells. CONCLUSION: DCs from patients with CHB can induce HBV antigen-specific T cell reactions, including secretion of cytokines essential for HBV clearance and for killing cells infected with HBV. | Ruo-BingLi Hong-SongChen YaoXie RanFei XuCong DongJiang Song-XiaWang Laiwei YuWang | 2004 | World Journal of Gastroenterology2004，10，11： | 8 |
| 3 | Blunted nitric oxide regulation in Tibetans under high-altitude hypoxia显示文摘Nitric oxide(NO) is an important molecule for vasomotor tone, and elevated NO signaling was previously hypothesized as a unique and adaptive physiological change in highland Tibetans. However, there has been lack of NO data from Tibetans living at low altitude and lowlander immigrants living at high altitude, which is crucial to test this hypothesis. Here, through cross-altitude(1990–5018 m) and cross-population(Tibetans and Han Chinese) analyses of serum NO metabolites(NOx) of 2086 individuals, we demonstrate that although Tibetans have a higher serum NOx level compared to lowlanders, Han Chinese immigrants living at high altitude show an even higher level than Tibetans. Consequently, our data contradict the previous proposal of increased NO signaling as the unique adaptive strategy in Tibetans.Instead, Tibetans have a relatively lower circulating NOx level at high altitude. This observation is further supported by data from the hypoxic experiments using human umbilical vein endothelial cells and gene knockout mice. No difference is detected between Tibetans and Han Chinese for endothelial nitric oxide synthase(e NOS), the key enzyme for circulating NO synthesis, suggesting that e NOS itself is unlikely to be the cause. We show that other NO synthesis-related genes(e.g. GCH1) carry Tibetan-enriched mutations significantly associated with the level of circulating NOx in Tibetans. Furthermore, gene network analysis revealed that the downregulation and upregulation of NOx is possibly achieved through distinct pathways.Collectively, our findings provide novel insights into the physiological and genetic mechanisms of the evolutionary adaptation of Tibetans to high-altitude hypoxia. | Yaoxi He Xuebin Qi Ouzhuluobu Shiming Liu Jun Li Hui Zhang Baimakangzhuo Caijuan Bai Wangshan Zheng Yongbo Guo Duojizhuoma Baimayangji Dejiquzong Bianba Gonggalanzi Yongyue Pan Qula Kangmin Cirenyangji Wei Guo Yangla Yi Peng Xiaoming Zhang Kun Xiang Zhaohui Yang Liangbang Wang Gengdeng Yanfeng Zhang Tianyi Wu Bing Su Chaoying Cui | 2018 | National Science Review2018，5，4： | 7 |
| 4 | De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation显示文摘Structural variants(SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome(ZF1), with a contig N50 length of 24.57 mega-base pairs(Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps(0.25 Mb in total length) in the reference human genome(GRCh38). Markedly, we detected17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid(Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1(1.32%–1.53%) compared to other East Asian genomes(0.70%–0.98%),reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence-a662-bp intronic insertion in the SCUBE2 gene-is enriched and associated with better lung function(the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies. | Ouzhuluobu Yaoxi He Haiyi Lou Chaoying Cui Lian Deng Yang Gao Wangshan Zheng Yongbo Guo Xiaoji Wang Zhilin Ning Jun Li Bin Li Caijuan Bai Baimakangzhuo Gonggalanzi Dejiquzong Bianba Duojizhuoma Shiming Liu Tianyi Wu Shuhua Xu Xuebin Qi Bing Su | 2020 | National Science Review2020，7，2： | 4 |
| 5 | Complex permittivity, permeability, and microwave absorption of barium ferrite by citrate sol-gel process显示文摘BaFe12O19 hexaferrites were prepared by citrate sol-gel process. The formation temperature was much lower thanthat of the conventional ceramic method. The complex dielectric constant and complex permeability of hexaferrite-paraffinwax composites had been measured by the transmission/reflection coaxial line method in the range from 200 MHz to 6 GHz.The measurement results showed that both the complex dielectric constant and dielectric loss exhibited no significant varia-tion with the measuring frequency increase. It also showed that the real part of permeability decreased as the frequency in-creasing for all samples, the resonance phenomena due to domain wall resonance was observed in μ' spectra, the calciningtemperature strongly affects the microwave properties of BaFe12O19 ferrites, the higher the calcining temperature, the lowerthe resonance frequency. | ZHANGHaijun WUMingzhong YAOXi ZHANGLiangying | 2003 | Rare Metals2003，22，2： | 3 |
| 6 | Cd^(2+) removal from wastewater by sulfate reducing bacteria with an anaerobic fluidized bed reactor显示文摘Cd2+removalfromwastewaterbysulfatereducingbacteriawithananaerobicfluidizedbedreactorMaXiaohang,HuaYaoxi,JiangFengInstituteofM... | Ma Xiaohang, Hua Yaoxi, Jiang Feng Institute of Microbiology of Zhejiang Province, Hangzhou 310012, China Liu Jian Environmental Protection Institute of Zhejiang Province, Hangzhou 310007, China Ye Xieming Metallurgical Institute of Zheji | 1997 | Journal of Environmental Sciences1997，9，3： | 3 |
| 7 | Flow analysis and design of three-dimensional wind tunnel contractions 显示文摘 | SU Yaoxi | 1991 | AIAA Journal1991，29，11： | 1 |
| 8 | 显示文摘 | ChouXiujian(-修建) ZhaiJiwei(翟继伟) Yaoxi(姚熹) | 2007 | 硅酸盐学报2007，35，： | 1 |
| 9 | Experimentalresearchontheeffectofbloodsug-arinComusofficinalisdifferentextract显示文摘 | YaoXY YangY DongFY | 1997 | 北京中医1997，，5： | 1 |
| 10 | 显示文摘 | WangJiangying(王疆瑛) YaoXi(姚熹) ZhangLiangying(张良莹) | 2004 | 功能材料2004，6，35： | 1 |
| 11 | Flow analysis and design of three-dimensional wind tunnel contractions显示文摘 | Su Yaoxi | 1991 | AIAA jom~al1991，29，： | 1 |
| 12 | Electrical nonuniformity of grain boundaries within ZnO varistors显示文摘 | SUN Hong ZHANGLiangying YAOXi | 1993 | Journal of the American Ceramic Society:1993，76，5： | 1 |
| 13 | Adsorption voltam- metric determination of μg · L^-1 level formaldehyde via in situ derivatization with Girard' s reagent显示文摘 | Wing Hongchan TIAN Yaoxie | 1997 | Anal Chim Acta1997，339，12： | 1 |
| 14 | Polarization and depolarization behavior of hot pressed lead lanthanum zireonate titanate ceramics显示文摘 | YAOXi CHEN Zhi-li CROSS L E | 1983 | Journal of Applied Physics1983，54，： | 1 |
| 15 | 显示文摘 | HuangHui(黄晖) LuoHongjie(罗宏杰) YaoXi(姚熹) | 2002 | 物理学报)2002，51，： | 1 |
| 16 | Exploring the primate genome:Unraveling the mysteries of evolution and human disease显示文摘Nonhuman primates(NHPs),our closest relatives,represent one of the most successful lineages of adaptive radiation in mammals.The primate order contains several hundred species living in varied ecological niches with phenotypic diversity.However,what we know about NHPs is still limited because of genome diversity.To address this gap,the Primate Genome Project(PGP)consortium recently made a comprehensive sequencing effort. | Yaoxi He Yafei Mao | 2023 | The Innovation2023，4，4： | 0 |
| 17 | Seasonality and Sex-Biased Fluctuation of Birth Weight in Tibetan Populations显示文摘Birth weight(BW)is a key determinant of infant mortality.Previous studies have reported seasonal fluctuation of BW.However,the responsible environmental factors remain disputable.High-altitude environment provides a great opportunity to test the current hypotheses due to its distinctive climate conditions.We collected BW data of~9000 Tibetan singletons born at Lhasa(elevation:3660 m)from 2014 to 2018.Using regression models,we analyzed BW seasonality of highland Tibetans.Multivariate models with meteorological factors as independent variables were employed to examine responsible environmental factors accounting for seasonal variation.We compared BW,low-BW prevalence and sex ratio between high-land and lowland populations,and we observed a significant seasonal pattern of BW in Tibetans,with a peak in winter and a trough in summer.Notably,there is a marked sex-biased pattern of BW seasonality(more striking in males than in females).Sunlight exposure in the 3rd trimester and barometric pressure exposure in the 2nd trimester are significantly correlated with BW,and the latter can be explained by seasonal change of oxygen partial pressure.In particular,due to the male-biased BW seasonality,we found a more serious BW reduction and higher prevalence of low-BW in males,and a skewed sex ratio in highlanders.The infant BW of highland Tibetans has a clear pattern of seasonality.The winter BW is larger than the summer BW,due to the longer sunlight exposure during the late-trimester.Male infants are more sensitive to hypoxia than female infants during the 2nd trimester,leading to more BW reduction and higher mortality. | Yaoxi He Jun Li Tian Yue Wangshan Zheng Yongbo Guo Hui Zhang Li Chen Chunxia Li Hongyan Li Chaoying Cui Ouzhuluobu Xuebin Qi Bing Su | 2022 | Phenomics2022，2，1： | 0 |
| 18 | Infection and Immunity of Herpes Simplex Virusin the Culture System sof Lym phocytes and Monocyte-Macrophages显示文摘The study was made by the method of experimental infection using cultured cells in vitro. The characteristic of HSV infection of the cells and the effects of immunity factors on the infection were analyzed by cytopathic effect, double antibodies sandwich ELISA for immunoglobulin yield, hemolytic plaque assay for specific SRBC antibody forming cells, microcytopathy assay for viral titer, IFA for viral antigen and PCR for viral DNA.The results were as follows: ①A series of models of HSV infection were established, including the model of HSV 1 persistent infection of Raji cells, the model of acute and cytocidal HSV 1 infection of HSB\-2 cells, the models of temporary persistent HSV 1 infection of LPS stimulated U\-\{937\} cells and murine peritoneal macrophages and the models of inhibitions both of IgG synthesis and specific SRBC antibody production by HSV 1 infection of human tonsillar lymphocytes activated by PWM stimulating. ②According to the characteristic of HSV infection of lymphocyte and monocyte macrophage, it was reasonable that stimulating with antigen, mitogen, LPS, or inflammatory factors might make lymphocytes and/or monocyte macrophages become permissive cells of replicative HSV infection, or might activate the latent virus, resulting in HSV dissemination by blood circulation. ③It was proved that IFN α, IFN γ, TNF, M CSF, GM CSF and IL 3 have an inhibitory effect on HSV replication in lymphocyte and monocyte macrophage, respectively and reversed the replicative enhancement activity of LPS, suggesting that applications of these immunity factors favour lymphocytes and monocyte macrophages with a resistance to HSV replicative infection and with an inhibition of latent virus reactivation in vivo, and therefore, it might be helpful for preventing the virus from dissemination by blood circulation.\; | Ji Xiaohui(季晓辉) Yao Kun(姚 ) Zhou Yaoxi(周瑶玺) Zhang Ming(张 明) Wang Minglun(王明伦) Cao Zhihui(曹志惠) Sun Banghua(孙邦华) Li Huandi(李焕娣) Ding Runing(丁如宁) Chen Shenbao(陈慎宝) | 1999 | The Journal of Biomedical Research1999，23，2： | 0 |
| 19 | Preparation and microwave hexaferrites by a sol-gel process显示文摘Ba4ZnzCo2_zFe36O60 hexaferrites with Z = 2.0, 1.5, 1.0, 0.5, and 0.0 were prepared by a citrate sol-gel process.The complex dielectric constant and complex permeability of hexaferrite-paraffin wax composites were measured in the range from 0.1 to 6.0 GHz. Measured results showed that both the complex dielectric constant and the dielectric loss exhibited no significant change as the measuring frequency increasing except for the sample with Z = 0.0. The real part of permeability decreased with increasing frequency for all samples in the whole measuring frequency range, and the natural resonance phenomena were observed in allμ' spectrum but Co2U. The results also indicate that the content of zinc closely affects the microwave properties of Ba4ZnzCo2_zFe36O60 felTites, and the greater the zinc content, the lower the natural resonance frequency is. | ZHANGHaijun JIAXiaolin YAOXi ZHANGLiangying | 2004 | Rare Metals2004，23，1： | 0 |
| 20 | 叶绿体到细胞核逆行信号调控拟南芥microRNA生成显示文摘文章简介叶绿体是植物细胞重要的细胞器,它不仅是光合作用的中心,而且是植物感受内源和外界环境刺激的感受器之一。叶绿体可以利用代谢过程中产生的小分子,通过逆行信号通路将环境刺激传递至细胞核,调控核内基因表达。在这项研究中,研究者通过正向遗传筛选获得了一个严重影响miRNA产生的突变体CUE1。 | 方晓峰 赵高展 Su Zhang Yaoxi Li Hanqing Gu Yan Li Qiao Zhao 戚益军 | 2020 | 科学新闻2020，，2： | 0 |
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