|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 2 | Tip-assisted ambient electric arc ionization mass spectrometry for rapid detection of trace organophosphorus pesticides in strawberry显示文摘In this study,an ambient mass spectrometry(AMS)based method was developed for rapid detection of organophosphorus pesticides in strawberry.This method combines an electric arc and a microsyringe tip to realize tip-assisted ambient electric arc ionization(TAAEAI).A high-voltage electric arc can be applied to the microsyringe tip to generate an electric field,which results in corona discharge at the microsyringe tip.The juiced strawberry sample loaded on the tip could be directly ionized with TAAEAI and then analyzed by a mass spectrometer.TAAEAI-MS was successfully applied to analyze 6 organophosphorus pesticides in three strawberry samples.Malathion and profenofos were detected from the investigated strawberry samples.This method could quantitatively determine the contents of organophosphorus pesticides in strawberry with high reproducibility,high precision,and high sensitivity.Sample matrices did not interfere with the pesticide analysis.The recoveries of organophosphorus pesticides spiked in strawberry samples varied between 82.6%and 116%with relative standard deviations(RSDs)less than 9.2%.The limits of detection(LODs)varied between 0.0124µg/g and 0.0245µg/g,while the limits of quantification(LOQs)varied between 0.0413µg/g and 0.0817µg/g.The coefficients of determination(R2)of the method were determined to be>0.995.The method established here may have potential application in the detection of organophosphorus pesticides in vegetables and fruits. | Zihan Ma Yuanji Gao Fengjian Chu Yunli Tong Yuwen He Yuan Li Zhan Gao Weiwei Chen Shuheng Zhang Yuanjiang Pan | 2022 | Chinese Chemical Letters2022，33，9： | 2 |
| 3 | Analysis of heat transfer and pressure drop for fin-and-tube heat exchangers with rectangular winglet-type vortex generators 显示文摘 | He Yaling Chu Pan Tao Wenquan Zhang Yuwen Xie Tao | 2013 | Applied Thermal Engineering2013，61，2： | 1 |
| 4 | Interfering with cellular signaling pathways enhances sensitization to combined sodium butyrate and GCV treatment in EBV-positive tumor cells显示文摘 | Yuwen He Shaohui Cai Ge Zhang Xiaoqing Li Liting Pan Jun Du | 2008 | Virus Research2008，，1： | 1 |
| 5 | Photocatalytic reduction of carbon dioxide on NiO/InTaO4 under visible light irradiation 显示文摘 | PAN PEIWEN CHEN YUWEN | 2007 | Catalysis Communications2007，8，15： | 1 |
| 6 | Genomic diversity and post-admixture adaptation in the Uyghurs显示文摘Population admixture results in genome-wide combinations of genetic variants derived from different ancestral populations of distinct ancestry, thus providing a unique opportunity for understanding the genetic determinants of phenotypic variation in humans. Here, we used whole-genome sequencing of 92 individuals with high coverage(30–60×) to systematically investigate genomic diversity in the Uyghurs living in Xinjiang, China(XJU), an admixed population of both European-like and East-Asian-like ancestry.The XJU population shows greater genetic diversity, especially a higher proportion of rare variants,compared with their ancestral source populations, corresponding to greater phenotypic diversity of XJU.Admixture-induced functional variants in EDAR were associated with the diversity of facial morphology in XJU. Interestingly, the interaction of functional variants between SLC24A5 and OCA2 likely influences the diversity of skin pigmentation. Notably, selection has seemingly been relaxed or canceled in several genes with significantly biased ancestry, such as HERC2–OCA2. Moreover, signatures of post-admixture adaptation in XJU were identified, including genes related to metabolism(e.g. CYP2D6), digestion(e.g.COL11A1), olfactory perception(e.g. ANO2) and immunity(e.g. HLA). Our results demonstrated population admixture as a driving force, locally or globally, in shaping human genetic and phenotypic diversity as well as in adaptive evolution. | Yuwen Pan Chao Zhang Yan Lu Zhilin Ning Dongsheng Lu Yang Gao Xiaohan Zhao Yajun Yang Yaqun Guan Dolikun Mamatyusupu Shuhua Xu | 2022 | National Science Review2022，9，3： | 1 |
| 7 | Interfering with cellular signaling pathways enhances sensitization to combined sodium butyrate and GCV treatment in EBV-positive tumor cells显示文摘 | Yuwen He Shaohui Cai Ge Zhang Xiaoqing Li Liting Pan Jun Du | 2008 | Virus Research2008，，1： | 1 |
| 8 | Strategies for improving adipose-derived stem cells for tissue regeneration显示文摘Adipose-derived stem cells(ADSCs)have promising applications in tissue regeneration.Currently,there are only a few ADSC products that have been approved for clinical use.The clinical application of ADSCs still faces many challenges.Here,we review emerging strategies to improve the therapeutic efficacy of ADSCs in tissue regeneration.First,a great quantity of cells is often needed for the stem cell therapies,which requires the advanced cell expansion technologies.In addition cell-derived products are also required for the development of‘cell-free’therapies to overcome the drawbacks of cell-based therapies.Second,it is necessary to strengthen the regenerative functions of ADSCs,including viability,differentiation and paracrine ability,for the tissue repair and regeneration required for different physiological and pathophysiological conditions.Third,poor delivery efficiency also restricts the therapeutic effect of ADSCs.Effective methods to improve cell delivery include alleviating harsh microenvironments,enhancing targeting ability and prolonging cell retention.Moreover,we also point out some critical issues about the sources,effectiveness and safety of ADSCs.With these advanced strategies to improve the therapeutic efficacy of ADSCs,ADSC-based treatment holds great promise for clinical applications in tissue regeneration. | Xin Yuan Li Li Haofan Liu Jing Luo Yongchao Zhao Cheng Pan Xue Zhang Yuwen Chen Maling Gou | 2022 | Burns & Trauma2022，10，1： | 1 |
| 9 | Formulation, preparation, and stability of intravenous bufadienolides‐loaded lipid microspheres显示文摘 | Yan Weng Cheng Pan Jia Meng Bin Tian Miaomiao Xi Zhifu Yang Yuwen Li Yue Guan Xing Tang Aidong Wen | 2012 | Eur. J. Lipid Sci. Technol2012，，10： | 1 |
| 10 | Simulation of the water level influence on the difference within the water-tube tiltmeter in Shuangyang Lake显示文摘This article analyzes the relationship between the water level and the water-tube tilting in Shuangyang lake,based on the differential deformation features reflected by the NS and EW components of the water-tube tiltmeter.The results show a good spatiotemporal consistency between the variation of water level and the NS tilt component,which is considered to be affected by the magnitude and duration of the water level variation in Shuangyang Lake.The article uses Landsat remote sensing image data to extract the water boundary of Shuan-gyang Lake,and takes advantage of the finite element numerical simulation method to build three-dimensional models for different geological structural conditions of the Shuangyang seismostation.The simulation results show that when the underground medium is granite,the effect of water level variation on the vertical displacement of the surface is non-directional.With a 50-m soil layer in Model 2,the simulated NS tilt variation is equivalent to the actual observed water-tube tiltmeter NS component when the water level variation is 0.44 m and 0.8m.When the variation of water level reaches 2.0m,the simulation result of the NS component is 79.6 ms,which is slightly larger than the observed result of 60.32 ms.However,the simulation results show that the variation of the EW component is significantly smaller than that of the NS one.Due to the fact that the Shuan-gyang lake is long in the NS direction and short in the EW direction,the existence of the soil layer tends to generate ground deformation along the NS direction in the vicinity of the lake after the increase of water level,thereby resulting in the difference of the ground deformation in the two directions. | Chong Yue Chuncheng Tang Wei Yan Xiaodong Pan Xueme Li Yuwen Tan Zongfeng Zhang Tianlong Yu | 2021 | Earthquake Research Advances2021，1，2： | 1 |
| 11 | Expression profiles of east–west highly differentiated genes in Uyghur genomes显示文摘It remains unknown and debatable how European-Asian–differentiated alleles affect individual phenotypes.Here,we made the first effort to analyze the expression profiles of highly differentiated genes with eastern and western origins in 90 Uyghurs using whole-genome(30×to 60×)and transcriptome data.We screened 921872 east–west highly differentiated genetic variants,of which~4.32%were expression quantitative trait loci(e QTLs),~0.12%were alternative splicing quantitative trait loci(s QTLs),and~0.12%showed allele-specific expression(ASE).The 8305 highly differentiated eQTLs of strong effects appear to have undergone natural selection,associated with immunity and metabolism.European-origin alleles tend to be more biasedly expressed;highly differentiated ASEs were enriched in diabetes-associated genes,likely affecting the diabetes susceptibility in the Uyghurs.We proposed an admixture-induced expression model to dissect the highly differentiated expression profiles.We provide new insights into the genetic basis of phenotypic differentiation between Western and Eastern populations,advancing our understanding of the impact of genetic admixture. | Zhilin Ning Xinjiang Tan Yuan Yuan Ke Huang Yuwen Pan Lei Tian Yan Lu Xiaoji Wang Ruicheng Qi Dongsheng Lu Yajun Yang Yaqun Guan Dolikun Mamatyusupu Shuhua Xu | 2023 | National Science Review2023，10，4： | 0 |
| 12 | Performance Comparison of Computational Methods for the Prediction of the Function and Pathogenicity of Non-coding Variants显示文摘Non-coding variants in the human genome significantly influence human traits and complex diseases via their regulation and modification effects.Hence,an increasing number of computational methods are developed to predict the effects of variants in human non-coding sequences.However,it is difficult for inexperienced users to select appropriate computational methods from dozens of available methods.To solve this issue,we assessed 12 performance metrics of 24 methods on four independent non-coding variant benchmark datasets:(1)rare germline variants from clinical relevant sequence variants(ClinVar),(2)rare somatic variants from Catalogue Of Somatic Mutations In Cancer(COSMIC),(3)common regulatory variants from curated expression quantitative trait locus(eQTL)data,and(4)disease-associated common variants from curated genomewide association studies(GWAS).All 24 tested methods performed differently under various conditions,indicating varying strengths and weaknesses under different scenarios.Importantly,the performance of existing methods was acceptable for rare germline variants from ClinVar with the area under the receiver operating characteristic curve(AUROC)of 0.4481–0.8033 and poor for rare somatic variants from COSMIC(AUROC=0.4984–0.7131),common regulatory variants from curated eQTL data(AUROC=0.4837–0.6472),and disease-associated common variants from curated GWAS(AUROC=0.4766–0.5188).We also compared the prediction performance of 24 methods for non-coding de novo mutations in autism spectrum disorder,and found that the combined annotation-dependent depletion(CADD)and context-dependent tolerance score(CDTS)methods showed better performance.Summarily,we assessed the performance of 24 computational methods under diverse scenarios,providing preliminary advice for proper tool selection and guiding the development of new techniques in interpreting non-coding variants. | Zheng Wang Guihu Zhao Bin Li Zhenghuan Fang Qian Chen Xiaomeng Wang Tengfei Luo Yijing Wang Qiao Zhou Kuokuo Li Lu Xia Yi Zhang Xun Zhou Hongxu Pan Yuwen Zhao Yige Wang Lin Wang Jifeng Guo Beisha Tang Kun Xia Jinchen Li | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 13 | Solve the False-Positive Problem of the Mimic System Based on the Best Mimic Component Set显示文摘At present,there is a problem of false positives caused by the too vast mimic scope in mimic transformation technology.Previous studies have focused on the“compensation”method to deal with this problem,which is expensive and cannot fundamentally solve it.This paper provides new insights into coping with the situation.Firstly,this study summarizes the false-positive problem in the mimic transformation,analyzes its possible harm and the root causes.Secondly,three properties about the mimic scope are proposed.Based on the three properties and security quantification technology,the best mimic component set theory is put forward to solve the false-positive problem.There are two algorithms,the supplemental method and the subtraction method.The best mimic component set obtained by these two algorithms can fundamentally solve the mimic system’s false-positive problem but reduce the cost of mimic transformation.Thus make up for the lack of previous researches. | Yuwen Shao Zheng Zhang Xiaomei Wang Chuanxing Pan Jiangxing Wu | 2022 | China Communications2022，19，5： | 0 |
| 14 | Double-branch fusion network with a parallel attention selection mechanism for camouflaged object detection显示文摘To meet the challenge of camouflaged object detection(COD),which has a high degree of intrinsic similarity between the object and background,this paper proposes a double-branch fusion network(DBFN)with a parallel attention selection mechanism(PASM).In detail,a schismatic receptive field block(SRF)combined with an attention mechanism for low-level information is performed to learn texture features in one branch,and an integration of the SRF,a hybrid attention mechanism(HAM),and a depth feature polymerization module(DFPM)is employed for high-level information to extract detection features in the other branch.Then,both texture features and detection features are input into the PASM to acquire selective expression matrices.Eventually,the final result is obtained after further selective matrix optimization with atrous spatial pyramid pooling(ASPP)and a residual channel attention block(RCAB)being applied serially.Experimental results on three public datasets verify that our method outperforms the state-of-the-art methods in terms of four evaluation metrics,i.e.,mean absolute error(MAE),weighted Fβmeasure(Fβω),structural measure(S_(α)),and E-measure(E_(φ)) | Junjiang XIANG Qing PAN Zhengrong ZHANG Songnian FU Yuwen QIN | 2023 | Science China(Information Sciences)2023，66，6： | 0 |
| 15 | Lineage-specific positive selection on ACE2 contributes to the genetic susceptibility of COVID-19显示文摘The Angiotensin-Converting Enzyme-2(ACE2)gene,located on Xp22.2,attracts a great deal of attention because the protein it encodes is believed to be the functional cellular receptor for the new coronavirus(SARS-CoV-2).However,recent studies are controversial,especially concerning the intrinsic link between ACE2 diversity and COVID-19 susceptibility.Here,we conduct a population genetic study on ACE2 in6354 individuals representing 210 present-day populations and 5329 individuals of ancient or archaic groups.We dissected the genetic architecture ofACE2 and identified two major haplogroups(hg)in East Asians,i.e.ACE2-hgl(43%)and ACE2-hg2(53%),while other populations harbor more diverse A CE2-hgs.Accordingly,there was a significant loss of ACE2 common variations in East Asians in contrast to the X-chromosome-wide and genome-wide patterns.Notably,association analysis between ACE2-hgs and COVID-19 severity in 1229 Han Chinese individuals with various levels of COVID-19 severity showed a higher risk ofACE2-hgl(odds ratio=1.56,P<0.01)and a lower risk of ACE2-hg2(odds ratio=0.65,P<0.01).Interestingly,ACE2-hgl is in strong linkage disequilibrium with rs1849863-C,which is an assumed risk factor of elevated plasma ACE2 level and is related to a higher risk of COVID-19 severity,hospitalization and infection.Strikingly,remarkable signatures of positive selection were detected,especially on ACE2-hg2,and were traced back to 100000 years ago(but rose to a strong level during the Bronze Age,5000~3000 years ago,in East Asians).The selection pressures could have stemmed from multiple sources,but pre-COVID-19 viral epidemics and pandemics might have been potential driving forces,which consequently contributed to the genetic susceptibility to COVID-19 within and between populations. | Yuwen Pan Panhong Liu Fang Wang Peng Wu Fanjun Cheng Xin Jin Shuhua Xu | 2022 | National Science Review2022，9，9： | 0 |
| 16 | Signaling Pathways Associated with Cancer Stem Cells Play a Significant Role in Immunotherapy Resistance显示文摘Cancer stem cells(CSCs)are a subpopulation of tumor cells with properties of self-renewal,pluripotency,plasticity,and differentiation,and are associated with various aberrantly stimulated signaling pathways.They are responsible for tumor recurrence,distant metastasis,and drug resistance,thus inducing poor prognosis.Immunotherapy has achieved encouraging results.However,the resistance associated with its clinical application is a persistent problem in clinical and scientific researches.Increasing evidence shows that signaling pathways associated with CSCs mediate immunotherapy resistance.This review highlights the link between them,and focuses on the underlying mechanism so as to provide potential strategies and approaches for the development of new targets against the immune resistance challenge. | Yajuan Zhu# Yuwen Zhou Yao Xie Pan Song Xuelei Ma | 2019 | Journal of Oncology Research2019，1，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员