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| 1 | The Coix Genome Provides Insights into Panicoideae Evolution and Papery Hull Domestication显示文摘Coix is a grass crop domesticated as early as the Neolithic era.It is still widely cultivated for both highly nutritional food and medicinal use.However,the genetic study and breeding of this crop are hindered by the lack of a sequenced genome.Here,we report de novo sequencing and assembly of the 1619-Mb genome of Coix,and annotation of 75.39%repeats and 39629 protein-coding genes.Comparative genomics analysis showed that Coix is more closely related to sorghum than maize,but intriguingly only Coix and maize had a recent genome duplication event,which was not detected in sorghum.We further constructed a genetic map and mapped several important traits,especially the strength of hull.Selection of papery hull(thin:easy dehulling)from the stony hull(thick:difficult dehulling)in wild progenitors was a key step in Coix domestication.The papery hull makes seed easier to process and germinate.Anatomic and global transcriptome analysis revealed that the papery hull is a result of inhibition of cell division and wall biogenesis.We also successfully demonstrated that seed hull pressure resistance is controlled by two major quantitative trait loci(QTLs),which are associated with hull thickness and color,respectively.The two QTLs were further fine mapped within intervals of 250 kb and 146 kb,respectively.These resources provide a platform for evolutionary studies and will facilitate molecular breeding of this important crop. | Chao Guo Yanan Wang Aiguo Yang Jun He Chaowen Xiao Shanhua Lv Fengming Han Yibing Yuan Yuan Yuan Xiaolong Dong Juan Guo YawenYang-Hailan Liu Ningzhi Zuo Yaxi Hu Kangxu Zhao Zhengbo Jiang Xing Wang Tingting Jiang Yaou Sherf Moju Cao Yuan Wang Zhaobo Long Tingzhao Rong Luqi Huang Shufeng Zhou | 2020 | Molecular Plant2020，13，2： | 11 |
| 2 | QTL mapping for grain number per spikelet in wheat using a high-density genetic map显示文摘Grain number per spikelet (GNS) is a key determinant of grain yield in wheat.A recombinant inbred line population comprising 300 lines was developed from a cross between a high GNS variety H461 and Chinese Spring from which the reference genome assembly of bread wheat was obtained.Both parents and the recombinant inbred lines were genotyped using the wheat 55K single nucleotide polymorphism(SNP) array.A high-density genetic map containing 21,197 SNPs was obtained.These markers covered each of the 21 chromosomes with a total linkage distance of 3792.71 c M.Locations of these markers in this linkage map were highly consistent with their physical locations in the genome assembly of Chinese Spring.The two parents and the whole RIL population were assessed for GNS in two consecutive years at two different locations.Based on multi-environment phenotype data and best liner unbiased prediction values,three quantitative trait loci (QTL) for GNS were identified.One of them located on chromosomes 2B and the other two on 2D.Phenotypic variation explained by these loci varied from 3.07%to26.57%.One of these QTL,QGns.sicau-2D-2,was identified in each of all trials conducted.Based on the best linear unbiased prediction values,this locus explained 19.59%–26.57%of phenotypic variation.A KASP(Kompetitive Allele-Specific PCR) marker closely linked with this locus was generated and used to validate the effects of this locus in three different genetic backgrounds.The identified QTL and the KASP marker developed for it will be highly valuable in fine-mapping the locus and in exploiting it for markerassisted selection in wheat breeding programs. | Yu Lin Xiaojun Jiang Haiyan Hu Kunyu Zhou Qing Wang Shifan Yu Xilan Yang Zhiqiang Wang Fangkun Wu Shihang Liu Caixia Li Mei Deng Jian Ma Guangdeng Chen Yuming Wei Youliang Zheng Yaxi Liu | 2021 | The Crop Journal2021，9，5： | 4 |
| 3 | MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1显示文摘Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious strategies.Exosomes combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound treatment.Here,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound healing.Silk fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were constructed.The designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac protein.Gluc signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of SGM-Exo.Moreover,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in miR146a-Exos.Superior to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and neovascularization.The transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP treatment.Here,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering. | Qiankun Li Wenzhi Hu Qilin Huang Jie Yang Bingmin Li Kui Ma Qian Wei Yaxi Wang Jianlong Su Mengli Sun Shengnan Cui Rungong Yang Haihong Li Xiaobing Fu Cuiping Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 2 |
| 4 | Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates显示文摘Fluorescence probes with aggregation-induced emission(AIE)characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution.However,the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation.Here,we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green(ICG)during a study period of 36 days to demonstrate AIE probes are nontoxic.Furthermore,through bright and nontoxic AIE probes and fluorescence imaging in the second window(NIR-II,1,000-1,700 nm),we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates,breaking the current limitation of millimeter-deep NIR-II fluorescence imaging.Our important findings,i.e.,nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates,may facilitate successful translation of AIE probes in clinical trials. | Zonghai Sheng Yaxi Li Dehong Hu Tianliang Min Duyang Gao Jen-Shyang Ni Pengfei Zhang Yuenan Wang Xin Liu Kai Li Hairong Zheng Ben Zhong Tang | 2020 | Research2020，，1： | 1 |
| 5 | Sensory protection to enhance functional recovery following proximal nerve injuries:current trends显示文摘Proximal nerve injury can lead to devastating functional impairment.Because axonal regeneration is slow,timely reinnervation of denervated muscle does not occur.These denervated muscles atrophy and lose function.Sensory protection is a surgical technique thought to prevent denervated muscle impairment using local sensory nerves to provide trophic support to the muscle until motor nerves can regenerate,and neuromuscular junctions are reestablished.We performed a comprehensive literature search using multiple databases to find primary articles reporting on the outcomes and treatment of sensory protection.This paper reviews the three main approaches to sensory protection:(1)end-to-end neurorrhaphy,(2)end-to-side neurorrhaphy,and(3)direct muscle neurotization.It discusses the evidence supporting each technique and outlines goals for future investigations. | Boa Tram Nghiem Ian C.Sando Yaxi Hu Melanie GUrbanchek Paul S.Cederna | 2015 | Plastic and Aesthetic Research2015，2，1： | 0 |
| 6 | Effect of riboflavin deficiency on intestinal morphology,jejunum mucosa proteomics,and cecal microbiota of Pekin ducks显示文摘This study was to determine the effects of riboflavin deficiency(RD)on intestinal development,jejunum mucosa proteome,cecal short-chain fatty acids(SCFA)profiling,and cecal microbial diversity and community of starter Pekin ducks.Male white Pekin ducks(1 d old,n=240)were allocated into 2 groups,with 12 replicates and 10 birds per replicate in each group.For 21 d,all ducks had ad libitum access to either an RD or a riboflavin adequate(control,CON)diet,formulated by supplementing a basal diet with 0 or 10 mg riboflavin per kg of diet,respectively.Compared to the CON group,growth retardation,high mortality,and poor riboflavin status were observed in the RD group.Furthermore,RD reduced the villus height and the ratio of villus height to crypt depth of jejunum and ileum(P<0.05),indicating morphological alterations of the small intestine.In addition,dietary RD enhanced relative cecum weight and decreased cecal SCFA concentrations(P<0.05),including propionate,isobutyrate,butyrate,and isovalerate.The jejunum mucosa proteomics showed that 208 proteins were upregulated and 229 proteins were downregulated in the RD group compared to those in the CON group.Among these,RD mainly suppressed intestinal absorption and energy generation processes such as glycolysis and gluconeogenesis,fatty acid beta oxidation,tricarboxylic acid cycle,and oxidative phosphorylation,leading to impaired ATP generation.In addition,RD decreased the community richness and diversity of the bacterial community in the cecum of ducks.Specifically,RD reduced the abundance of butyrate-producing bacteria in the cecum(P<0.05),such as Eubacterium coprostanoligenes,Prevotella and Faecalibacterium.Dietary RD resulted in growth depression and intestinal hypofunction of Pekin ducks,which could be associated with impaired intestinal absorption and energy generation processes in intestinal mucosa,as well as gut microbiota dysbiosis.These findings contribute to our understanding of the mechanisms of intestinal hypofunction due to RD. | Yaxi Xu Bo Zhang Rui Zhao Kexin Gao Suyun Liang Yongbao Wu Yongsheng Hao Dapeng Liu Zhanbao Guo Jian Hu Zhengkui Zhou Ming Xie Jing Tang | 2023 | Animal Nutrition2023，，1： | 0 |
| 7 | Strategies to improve genomic predictions for 35 duck carcass traits in an F2 population显示文摘Background Carcass traits are crucial for broiler ducks,but carcass traits can only be measured postmortem.Genomic selection(GS)is an effective approach in animal breeding to improve selection and reduce costs.However,the performance of genomic prediction in duck carcass traits remains largely unknown.Results In this study,we estimated the genetic parameters,performed GS using different models and marker densi-ties,and compared the estimation performance between GS and conventional BLUP on 35 carcass traits in an F2 population of ducks.Most of the cut weight traits and intestine length traits were estimated to be high and moder-ate heritabilities,respectively,while the heritabilities of percentage slaughter traits were dynamic.The reliability of genome prediction using GBLUP increased by an average of 0.06 compared to the conventional BLUP method.The Permutation studies revealed that 50K markers had achieved ideal prediction reliability,while 3K markers still achieved 90.7%predictive capability would further reduce the cost for duck carcass traits.The genomic relationship matrix nor-malized by our true variance method instead of the widely used 2pi(1-pi)could achieve an increase in prediction reliability in most traits.We detected most of the bayesian models had a better performance,especially for BayesN.Compared to GBLUP,BayesN can further improve the predictive reliability with an average of 0.06 for duck carcass traits.Conclusion This study demonstrates genomic selection for duck carcass traits is promising.The genomic prediction can be further improved by modifying the genomic relationship matrix using our proposed true variance method and several Bayesian models.Permutation study provides a theoretical basis for the fact that low-density arrays can be used to reduce genotype costs in duck genome selection. | Wentao Cai Jian Hu Wenlei Fan Yaxi Xu Jing Tang Ming Xie Yunsheng Zhang Zhanbao Guo Zhengkui Zhou Shuisheng Hou | 2023 | Journal of Animal Science and Biotechnology2023，14，5： | 0 |
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