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| 1 | Characterization of humic acids extracted from the sediments of the various rivers and lakes in China显示文摘The humic acids (HAs) isolated from the sediments of the various rivers,lakes,and reservoirs in China were studied using elemental analyzer,fourier transform infrared (FT-IR),and CP/MAS 13C nuclear magnetic resonance (NMR) spectroscopy.The results showed that the HAs were characterized by some common chemical and physicochemical properties,but they also pose some differences in the C-containing functional groups.The C/N,C/H,O/C,and O/H ratios differ widely for the various HAs,showing that the elemental composition of the HAs from the various sediments was different due to the different environmental conditions.All HAs show similar spectra with different intense absorbance in the region of 4000-2000 cm-1,suggesting that they have very similar structures and functional groups.The absorbance region between 1900 and 400 cm-1 also showed similar spectra with different intense absorbance,different relative intensities,and some relevant differences for the various HAs.The total aromaticities for the six HAs varied from 23.1% to 41.8%.The differences in the elemental composition and functional groups for the various HAs were attributed to their different biogeochemical origins.The HAs in the sediments from Taihu Lake (one freshwater shallow lake with heavy eutrophication),the Pearl River (the tropical river),and the Liao River (located at the joint of the temperate zone and cold-temperate zone) showed different structural properties due to their different geographical and climate zones.These different properties for the six HAs were expected to affect the sorption,distributions,and fates of heavy metals and organic chemicals. | Mengchang HE Chunye LIN | 2008 | Journal of Environmental Sciences2008，20，11： | 35 |
| 2 | GIAHS Project and Its Implementation in China显示文摘GIAHS Project and Concept In 2002,Food and Agriculture Organization of the United Nations(hereafter FAO)started an initiative for the conservation and adaptive management of Globally Important Agricultural Heritage Systems(abbreviated as GIAHS) to safeguard and support world’s agro-cultural | MIN Qingwen~1,SUN Yehong~(1,2) and SHI Yuanyuan~1 1.Institute of Geographic Sciences and Natural Resources Research,CAS,Beijing 100101,China 2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China | 2010 | Journal of Resources and Ecology2010，1，1： | 9 |
| 3 | Hyperlipidemia does not prevent the cardioprotection by postconditioning against myocardial ischemia/reperfusion injury and the involvement of hypoxia inducible factor-1α upregulation显示文摘Hyperlipidemia 在 ischemic 心疾病的发展被认为是一个独立风险因素,并且它能增加心肌的危险性到 ischemia/reperfusion (I/R ) 损害。Ischemic postconditioning (Postcon ) 被表明了稀释 I/R 处于正常条件导致的心肌的损害。但是 hyperlipidemic 动物上的 ischemic Postcon 的效果是未知的。组织缺氧可诱导的 factor-1 (HIF-1 ) 被表明了由 preconditioning 在 cardioprotection 起一个中央作用,它除了 Postcon 是保护的策略之一。这研究的目的是决定 Postcon 是否能在 hyperlipidemic 动物减少心肌的损害并且估计 HIF-1 是否涉及 Postcon 机制。在用高胖的饮食或正常饮食喂了 8 个星期以后,男 Wistar 老鼠经历了左前面的下降冠的吸藏因为 30 min 由灌注的 180 min 列在后面与或没有 Postcon。I/R 侮辱导致的有害索引包括了梗塞尺寸,血浆肌酸 kinase 活动和 caspase-3 活动。结果证明 hyperlipidemia 显著地提高了 I/R 导致的心肌的损害,当 Postcon 显著地减少了时在 normolipidemic 和 hyperlipidemic 老鼠的心肌的损害。而且, hyperlipidemia 和 I/R 支持了 HIF-1 表示。最重要地,我们第一次证明了 Postcon 进一步不仅在 normolipidemic 而且处于 hyperlipidemic 条件在 HIF-1 蛋白质水平导致了重要增加。因此, Postcon 减少 I/R 在正常和 hyperlipidemic 导致的心肌的损害动物,和 HIF-1 upregulation 可以在调停 Postcon 的 cardioprotective 机制包含。 | Huanxin Zhao Yehong Wang Ye Wu Xiaoyu Li Guangzhao Yang Xiurui Ma Rongmi Zhao Huirong Liu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 9 |
| 4 | Validation of glaucoma-like features in the rat episcleral vein cauterization model显示文摘 | Bai Yujing Zhu Yingting Chen Qin Xu Jing Marinko V. Sarunic Uri H. Saragovi Zhuo Yehong | 2014 | Chinese Medical Journal2014，，2： | 7 |
| 5 | miR-145 improves metabolic inflammatory disease through multiple pathways显示文摘Chronic inflammation plays a pivotal role in insulin resistance and type 2 diabetes,yet the mechanisms are not completely understood.Here,we demonstrated that serum LPS levels were significantly higher in newly diagnosed diabetic patients than in normal control.miR-145 level in peripheral blood mononuclear cells decreased in type 2 diabetics.LPS repressed the transcription of miR-143/145 cluster and decreased miR-145 levels.Attenuation of miR-145 activity by anti-miR-145 triggered liver inflammation and increased serum chemokines in C57BL/6 J mice.Conversely,lentivirus-mediated miR-145 overexpression inhibited macrophage infiltration,reduced body weight,and improved glucose metabolism in db/db mice.And miR-145 overexpression markedly reduced plaque size in the aorta in ApoE−/−mice.Both OPG and KLF5 were targets of miR-145.miR-145 repressed cell proliferation and induced apoptosis partially by targeting OPG and KLF5.miR-145 also suppressed NF-κB activation by targeting OPG and KLF5.Our findings provide an association of the environment with the progress of metabolic disorders.Increasing miR-145 may be a new potential therapeutic strategy in preventing and treating metabolic diseases such as type 2 diabetes and atherosclerosis. | Min He Nan Wu Man Cheong Leong Weiwei Zhang Zi Ye Rumei Li Jinyang Huang Zhaoyun Zhang Lianxi Li Xiao Yao Wenbai Zhou Naijia Liu Zhihong Yang Xuehong Dong Yintao Li Lili Chen Qin Li Xuanchun Wang Jie Wen Xiaolong Zhao Bin Lu Yehong Yang Qinghua Wang Renming Hu | 2020 | Journal of Molecular Cell Biology2020，12，2： | 7 |
| 6 | microRNA-21a-5p/PDCD4 axis regulates mesenchymal stem cell-induced neuroprotection in acute glaucoma显示文摘间充质的干细胞(MSC ) 被表明了为许多神经病学的疾病有有希望的治疗学的好处;然而,内在的机制糟糕被理解。这里,我们显示出 MSC 的那 intravitreal 注入在尖锐绿内障的一个老鼠模型的支持的网膜的中心细胞(RGC ) 幸存与 microglial 激活的重要抑制, TNF- , IL-1,和反应的氧种类的生产,以及 caspase-8 和 caspase-3 激活。在 vitro, MSC 禁止了两 caspase-8-mediated RGC apoptosis 和 microglial 激活,部分经由 stanniocalcin 的行动 1 (STC1 ) 。而且,我们发现了那 microRNA-21a-5p (miR-21 ) 和它的目标, PDCD4,为 STC1 生产和在 vitro 并且在 vivo 的 MSC 的 neuroprotective 性质是必要的。重要地, miR-21 overexpression 或 PDCD4 尖锐绿内障上的击倒的扩充调停 MSC 的 neuroprotective 效果。miR-21/PDCD4 轴由导致 MSC 分泌施加 neuroprotective 的 STC1 和另外的因素的以前未被认出的 neuroprotective 机制完成的这些数据热点。因此, modulating miR-21/PDCD4 轴可能是为尖锐绿内障和另外的神经病学的疾病的临床的处理的有希望的策略。 | Wenru Su Zuohong Li Yu Jia Yingting Zhu Wenjia Cai Peixing Wan Yingying Zhang Song Guo Zheng Yehong Zhuo | 2017 | Journal of Molecular Cell Biology2017，9，4： | 5 |
| 7 | The essential oil components of Cinnamomum cassia: an analysis under different thinning models of plantation Pinus massoniana显示文摘Thinning is an important activity employed in forest management. To date, studies have mainly focused on the effects of thinning on the growth of trees during the same thinning period. In this study, plantation Pinus massoniana Lamb. near maturity were thinned at varying intensities and an economically important species, Cinnamomum cassia Presl., was planted beneath the thinned canopy. The aim of the study was to explore the effects of the extent of thinning on the essential oil content and its components of C. cassia in different parts of the plant, as well as the economic feasibility of the P. massoniana–C.cassia management model. Thinning significantly reduced the oil yield in the bark and branches of C. cassia, but hardly impacted the oil yield from C. cassia leaves compared with pure C. cassia forest(CK). Among the different thinning treatments, both light(T.4) and extensive(T.1)thinning reduced the oil yield of C. cassia bark and new branches. The concentrations of the main aldehydes differed in different parts of the plant and were affected by the extent of thinning. The influence on cinnamaldehyde in the bark was minor, but was much greater in the branches and leaves. Both the oil yield and content of cinnamaldehyde showed that moderate(T.3) thinning was more favorable than other thinning models. These results not only provide a potentially promising model for the transformation of low–yield artificial pure forests of P. massoniana in the future, but also offer a reference for the management of artificial mixed stands. | Dayan Tao Yuanfa Li Daodiao Lu Yehong Luo Sufang Yu Shaoming Ye | 2016 | Journal of Forestry Research2016，27，3： | 5 |
| 8 | Group Connectivity and Group Colorings of Graphs -- A Survey显示文摘在 1950 年代, Tutte 介绍了理论没什么地方 -- 是的零流动调查地图的着色问题的一个工具,和他的大多数迷人推测在上没什么地方 -- 零流动。这些被纯毛织品等扩大了。在 1992 组织连接, nonhomogeneous 形式没什么地方 -- 零流动。让 G 2-edge-connected 是未受指导的图, A 一(添加剂) 可换群和 A *= A 吗?{ 0 } 。如果 G 有取向 D (G) ,图 G 是连接 A 的以便为每地图 b:V (G) 与令人满意的 S vV (G) b (v)= 0,有功能 f:E (G) 与 A * 以便为每顶点 v &te V (G) ,在指导进 v 的边上减 f 值的全部的数量从 v 外面指导的边上的 f 值的全部的数量等于 b (v) 。一张图的组着色从组连接的双概念产生。这些题目上有大量调查。这调查提供关于组连接和图的组着色的研究的一篇摘要。它包含下列节。1。没什么地方 -- 图的零个流动和组连接 2。完全的家庭和 A 减小 3。有边删除的减小,顶点删除并且切开顶点 4。作为组连接 5 的一个双概念组织着色。布鲁克斯定理,它的变化和双形式 6。平面图 7。图的组连接 7.1 张高度连接的图和折迭的图 7.2 个度条件 7.3 张互补的图图的 7.4 个产品有直径的 7.5 张图至多 2 7.6 张线图和没有爪的图 7.7 张三角形的图 7.8 爪分解和所有 Tutte | Hong-Jian LAI Xiangwen LI Yehong SHAO Mingquan ZHAN | 2011 | Acta Mathematica Sinica,English Series2011，27，3： | 4 |
| 9 | Residents’ Attitudes Towards Tourism in a Globally Important Agricultural Heritage Systems Pilot Site: a Case Study in China显示文摘Globally Important Agricultural Heritage Systems (GIAHS) is a wide program to promote the conservation and adap-tive management of such systems and their associated landscapes, biodiversity, knowledge systems and cultures. Rice-Fish Systems (RFS) in Longxian village of China, as a traditional agricultural system, was selected as one of the five pilot sites of GIAHS in 2005. Researchers have paid more attention to the dynamic conser-vation and adaptive management of RFS because it is under severe threats from global development challenges. Tourism is suggested to be brought into the RFS conservation program as an alternative industry. This paper presents a comparative study of residents in three types of farm household in Longxian, seeking attitudes to the RFS conservation and tourism development and ways in which such information may guide future strategies. Results show resi-dents, belonging to the household type in which most family mem-bers are abroad, have the most positive attitudes to RFS conserva-tion and tourism development; while residents, as other household type in which less than 50% of family members are abroad, are the least sensitive group in these three household types, due to the motivation of moving abroad weakening their enthusiasm to participate in the local activities. Implications are discussed in the context of how resident attitudes will affect the future manage-ment of GIAHS conservation and tourism development, and then measures are put forward to foster tourism cooperation and multi-stakeholders process establishment. | Sun Yehong Min Qingwen Cheng Shengkui | 2009 | Chinese Journal of Population,Resources and Environment2009，7，1： | 4 |
| 10 | Single Atom Alloy Preparation and Applications in Heterogeneous Catalysis显示文摘There have been remarkable progresses in manipulating heterogeneous catalysts' nanostructures in the past decade.The concept of single atom alloy (SAA) was firstly proposed in 2012 when researchers successfully stabilized single Pd atoms on the Cu(111) surface.However,earlier work in 2009,which focused on replacing one Au atom with a Pd atom in thiolate protected Au25 nanoclusters,could also be considered as the pioneer work of single atom alloy.Both kinds of single atom alloys exhibited the potential of maximum utilization of scarce elements and attractive catalytic performances.The well-defined structures of SAA catalysts make accurate modeling possible,which further realizes the rational design of single atom alloy catalysts.In this review,we summarize the research trajectory of single atom alloys as well as recent achievements in this field.We also Introduce several commonly adopted characterization methods for SAA catalysts such as scanning tunneling microscopy (STM),temperature programmed reaction (TPR),extended X-ray absorption fine structure (EXAFS) spectra,matrix assisted laser desorption/ionization mass spectrum (MALDI-MS) and differential pulse voltammetry (DPV).Through discussing recent progresses in SAA catalysts,we propose that future researches in this filed should be focused on exploring new kinds of metal nanocrystals and controlling the nanostructure of SAA even more precisely. | Jianyu Han jjanmin Lu Min Wang Yehong Wang Feng Wang | 2019 | Chinese Journal of Chemistry2019，37，9： | 4 |
| 11 | Tunable oxygen vacancy concentration in vanadium oxide as mass-produced cathode for aqueous zinc-ion batteries显示文摘Oxygen vacancy(Vö)is important in the modification of electrode for rechargeable batteries.However,due to the scarcity of suitable preparation strategy with controllable Vöincorporation,the impact of Vöconcentration on the electrochemical performances remains unclear.Thus,in this work,Vö-V_(2)O_(5)-PEDOT(VöVP)with tunable Vöconcentration is achieved via a spontaneous polymerization strategy,with the capability of mass-production.The introduction of poly(2,3-dihydrothieno-1,4-dioxin)(PEDOT)not only leads to the formation of Vöin V_(2)O_(5),but it also results in a larger interlayer spacing.The as-prepared Vö-V_(2)O_(5)-PEDOT-20.3%with Vöconcentration of 20.3%(denoted as VöVP-20)is able to exhibit high capacity of 449 mAh·g^(−1) at current density of 0.2 A·g^(−1),with excellent cyclic performance of 94.3% after 6,000 cycles.It is shown in the theoretical calculations that excessive Vöin V_(2)O_(5) will lead to an increase in the band gap,which inhibits the electrochemical kinetics and charge conductivity.This is further demonstrated in the experimental results as the electrochemical performance starts to decline when Vöconcentration increases beyond 20.3%.Thus,based on this work,scalable fabrication of high-performance electrode with tunable Vöconcentration can be achieved with the proposed strategy. | Yehong Du Xinyu Wang Juncai Sun | 2021 | Nano Research2021，14，3： | 4 |
| 12 | Peripheral Nerve and Transgene Cells Transplantation in the Treatment of Experimental Neuropathy of SD Rats显示文摘Purpose: To observe whether optic nerve can regenerate by peripheral nerve transplantation and GDNF gene transfer.Methods: Optic nerve was transected about 2 mm posterior to the eye to establish experimental models of optic neuropathy. Sciatic nerve was sutured to the sheath of injury optic nerve by a 10 - 0 nylon, pcDNA3-GDNF was smeared to the joint and injected to the vitreous cavity. The regenerated retinal ganglion cells axons were observed by HRP. The retina and optic nerve were observed by pathohistological methods.Results: Some retinal ganglion cells with regenerated axons could be seen a week after transplantation with large cell bodies. The cells could be identified by HRP. The regenerated nerve fibers grew to the transplanted sciatic nerve. Then the number of retinal ganglion cells with regenerated axons increased with time going. Only a few regenerated retinal ganglion cells could be seen two months after transplantation. The large retinal ganglion cells were seen to have plenty of | Jian Ge, Yanyan Li, Yehong Zhuo, Yan GuoZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 1998 | 眼科学报1998，14，3： | 3 |
| 13 | Characterization of Glucocorticoid Receptor on Lymphocytes in Chinese Patients with Glucocorticoid-induced Glaucoma显示文摘Purpose : We studied the pathogenesis of glucocorticoid-induced glucoma (GIG) through characterization of glucocorticoid receptor (GR) on lymphocytes in Chinese patients with GIG.Methods:By radioligand receptor binding followed by Scatchard analysis, the specific binding sites were characterized and quantitated for glucocorticoid receptors on peripheral blood lymphocytes obtained from patients with GIG and the control group.Results:The binding sites we detected were as follows; 12.7 ± 1.47 × 103 receptors per cell with a KD of 3.02 ± 0.62nmol/L in patients with GIG, 7.26 ± 0.45 × 103 receptors per cell with a KD of 3.03 ± 0.56nmol/L in the control group. The statistical difference of receptors per cell is significant between two groups (p < 0.05), patients with GIG having more GR binding sites, while the difference of Kd is not significant ( p > 0.05 ) . Conclusion: The preliminary findings suggest that patients with GIG are more sensitive to glucocorticoid and the increase of binding sites of | Yehong Zhuo, Jian Ge , Yan GuoZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences , Guangzhou 510060, China | 1998 | 眼科学报1998，14，3： | 3 |
| 14 | Determination of dioxin-like polychlorinated biphenyls in soil and moss from Fildes Peninsula,Antarctica显示文摘Dioxin-like polychlorinated biphenyls(PCBs) are a class of toxic and persistent chemicals that are often found in the environment.The determination of dioxin-like PCBs in Fildes Peninsula,Antarctica,is reported in this paper.Dioxin-like PCBs were widely distributed in Antarctic soil and moss with concentration of 2.23-27.2 pg/g in soil and 10.4-812 pg/g in moss.The highest concentrations were found in the Ardley Island area and the lowest concentrations on the west coast.The contribution of PCB-118 to the ∑12PCBs ranged from 36.0% to 69.6% in soil,and 17.2% to 43.4% in moss.The congener specific patterns in soil and moss were similar.Long-range atmospheric transport is thought to be the main source of PCBs detected on King George Island.The ∑12PCBs toxic equivalent(TEQ) in soil was 0.400×10?3 to 516×10?3 pg(TEQ)/g with a mean value of 147×10?3 pg(TEQ)/g.PCB-126 had the largest contribution(81.1%) to the ∑12PCBs TEQ in soil.The ∑12PCBs TEQ in moss ranged from 2.90×10?3 to 1.19 pg/g with a mean value of 0.482 pg/g.PCB-126 was also the largest contributor(91.4%) to the ∑12PCBs TEQ in moss.The mean TEQ in moss was about 40 times that in soil. | WANG Qiang SHI YeHong HU JianXin YAO ZiWei FANG XueKun DONG YiAn | 2012 | Chinese Science Bulletin2012，57，9： | 3 |
| 15 | Lysophosphatidic acid mediates the pathogenesis of psoriasis by activating keratinocytes through LPAR5显示文摘Dear Editor,Psoriasis is a common immune mediated,chronic inflammatory skin disease,which has been characterized by epidermal acanthosis,hyperkeratosis,parakeratosis and extensive infiltration of inflammatory cell.KCs have critical roles in skin innate and adaptive immune responses during the development of psoriasis,which produce large amounts of inflammatory mediators,such as antimicrobial peptides(e.g.,S100A8,9),proinflammatory cytokines(e.g,IL-6,IL-17A,C,TNF-α,)and chemokines(e.g,CXCL1,2),triggering innate or adaptive immune responses. | Li lei Bei Yan Panpan Liu Jie Li Chao Chen Wu Zhu Yehong Kuang Xiang Chen Cong Peng | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 3 |
| 16 | Cutaneous Hypersensitivity as an Indicator of Visceral Inflammation via C-Nociceptor Axon Bifurcation显示文摘Pain on the body surface can accompany disorders in the deep tissue or internal organs. However, the anatomical and physiological mechanisms are obscure.Here, we provided direct evidence of axon bifurcation in primary C-nociceptive neurons that innervate both the skin and a visceral organ. Double-labeled dorsal root ganglion(DRG) neurons and Evans blue extravasation were observed in 3 types of chemically-induced visceral inflammation(colitis, urocystitis, and acute gastritis) rat models.In the colitis model, mechanical hypersensitivity and spontaneous activity were recorded in vivo from doublelabeled C-nociceptive neurons in S1 or L6 DRGs. These neurons showed significantly enhanced responses to both somatic stimulation and colorectal distension. Our findings suggest that the branching of C-nociceptor axons contribute to cutaneous hypersensitivity in visceral inflammation. Cutaneous hypersensitivity on certain locations of the body surface might serve as an indicator of pathological conditions in the corresponding visceral organ. | Yehong Fang Shu Han Xiaoxue Li Yikuan Xie Bing Zhu Xinyan Gao Chao Ma | 2021 | Neuroscience Bulletin2021，37，1： | 3 |
| 17 | Rhodojaponin Ⅵ indirectly targets Cav2.2 channels via N-ethylmaleimide-sensitive fusion protein to alleviate neuropathic pain显示文摘Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed.Rhodojaponin Ⅵ,a grayanotoxin from Rhododendron molle,showed remarkable antinociceptive efficacy in models of neuropathic pain,but its biotargets and mechanisms are unknown.Given the reversible action of rhodojaponin Ⅵ and the narrow range over which its structure can be modified,we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin Ⅵ.N-Ethylmaleimide-sensitive fusion(NSF) was confirmed as the key target of rhodojaponin Ⅵ through biological and biophysical experiments.Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca2+current intensity,whereas rhodojaponin Ⅵ reversed the effects of NSF.In conclusion,rhodojaponin Ⅵ represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF. | Keliang Chen Tao Wang Yong Li Jun Wu Cheng-Xiao Zhao Sheng Liu Fengrun Sun Yehong Fang Jiahuan Hu Jinping Hu Chong-Jing Zhang Haibo Yu Chao Ma Shi-Shan Yu | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 2 |
| 18 | Inhibition of Muscular Nociceptive Afferents via the Activation of Cutaneous Nociceptors in a Rat Model of Inflammatory Muscle Pain显示文摘Topical irritants such as capsaicin(CAP),peppermint oil(PO),and mustard oil(MO)are effective in relieving inflammatory muscle pain.We investigated the effects of topical irritants in a rat model of inflammatory muscle pain produced by injecting complete Freund's adjuvant(CFA)into the tibialis anterior muscle.CFAinduced mechanical hypersensitivity and the spontaneous activity of muscular nociceptive afferents,and decreased weight-bearing of the hindlimb were relieved by topical application of CAP,PO,or MO on the skin overlying the inflamed muscle.The effects of topical irritants were abolished when applied to the skin on the ipsilateral plantar region or on the contralateral leg,or when the relevant cutaneous nerve or dorsal root was transected.Our results demonstrated that topical irritants may alleviate inflammatory muscle pain via activating cutaneous nociceptors and subsequently inhibiting the abnormal activity of muscular nociceptive neurons. | Yehong Fang Jie Zhu Wanru Duan Yikuan Xie Chao Ma | 2020 | Neuroscience Bulletin2020，36，1： | 2 |
| 19 | Aquaporin-1 Expressed in Cultured Human Trabecular Meshwork Cells显示文摘Objective:To determine if aquaporin-1 could be detected in cultures of human trabecularshwork cells. Methods: Using primers specific for aquaporin-1, reverse transcription combined withpolymerase chain reaction (RT-PCR) yielded a product and its size with total RNAprepared from the human trabecular meshwork cells. SDS-PAGE and immunoblottingwere also used in this study to detect the specific water channel.Results: The presence of this product and its size (298 base pairs) are consistent withthat of an aquaporin-1 message in these cells. A band of 28 kD in agreement with themolecular size of aquaporin-1 was showed in a film by immunoblotting.Conclusion: The presence of aquaporin-1 in human trabecular meshwork cells, thepredominant cell-type of the primary outflow region of the human eye, suggests that waterchannels may be involved in the movement of aqueous fluid out of the eye. In addition,the existence of aquaporin-1 on cultures of human trabecular meshwork cells provides anin vitro model to study the endogenous expression of aquaporin-1 and its possible role inthe regulation of aqueous outflow. | Mingkai Lin, Jian Ge, Yehong Zhuo, Yuqing Lan, Keming Yu, Jianliang ZhengZhongshan Ophthalmic center, Sun Yat-sen University, Guangzhou 510060, China | 2002 | Eye Science2002，18，1X： | 2 |
| 20 | Human umbilical cord mesenchymal stem cells for psoriasis:a phase 1/2a,single-arm study显示文摘Psoriasis is a common,chronic immune-mediated systemic disease that had no effective and durable treatment.Mesenchymal stem cells(MSCs)have immunomodulatory properties.Therefore,we performed a phase 1/2a,single-arm clinical trial to evaluate the safety and efficacy of human umbilical cord-derived MSCs(UMSCs)in the treatment of psoriasis and to preliminarily explore the possible mechanisms.Seventeen patients with psoriasis were enrolled and received UMsC infusions.Adverse events,laboratory parameters,PASl,and PGA were analyzed.We did not observe obvious side effects during the treatment and 6-month follow-up.A total of 47.1%(8/17)of the psoriasis patients had at least 40%improvement in the PASl score,and 17.6%(3/17)had no sign of disease or minimal disease based on the PGA score.And the efficiency was 25%(2/8)for males and 66.7%(6/9)for females.After UMSC transplantation(UMSCT),the frequencies of Tregs and CD4^(+)memory T cells were significantly increased,and the frequencies of T helper(Th)17 and CD4^(+)naive T cells were significantly decreased in peripheral blood(PB)of psoriasis patients.And all responders showed significant increases in Tregs and CD4^(+)memory T cells,and significant decreases in Th17 cells and serum IL-17 level after UMsCT.And baseline level of Tregs in responders were significantly lower than those in nonresponders.In conclusion,allogeneic UMSCT is safe and partially effective in psoriasis patients,and level of Tregs may be used as a potent biomarker to predict the clinical efficacy of UMSCT.Trial registration Clinical Trials NCT03765957. | Lamei Cheng Siqi Wang Cong Peng Xiao Zou Chao Yang Hua Mei Chuang Li Xian Su Na Xiao Qi Ouyang Mi Zhang Qiaolin Wang Yan Luo Minxue Shen Qun Qin Honglin Wang Wu Zhu Guangxiu Lu Ge Lin Yehong Kuang Xiang Chen | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 2 |
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