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| 1 | Rapid determination of pesticide residues in Chinese materia medica using QuEChERS sample preparation followed by gas chromatography-mass spectrometry显示文摘Pesticide residue analysis plays an important role in the quality control of Chinese materia medica.This paper reports the development and validation of an analytical method for the quantitative determination of the residues of 39 pesticides in 12 different matrices of Chinese materia medica.Sample preparation utilized the QuEChERS method with acetonitrile:1% aqueous acetic acid(9:1,v/v)as extraction solvent followed by sample clean-up by dispersive solid phase extraction using primary secondary amine sorbent and graphitized carbon black.Extracts were then analysed by gas chromatography coupled with electron impact mass spectrometry in the selected ion monitoring mode.Limit of detection(LOD)and limit of quantitation(LOQ)values were in the ranges 0.5-50 ng/g and 1-100 ng/g,respectively.The recoveries of the 39 pesticides were in the range 75-112% with precision(as relative standard deviation,RSD)<15%.The results show that the modified QuEChERS method allows rapid and sensitive analysis of multiple pesticide residues in Chinese materia medica. | Yichen Hu Li Wan Jinming Zhang Fang Yang Jiliang Cao | 2012 | Acta Pharmaceutica Sinica B2012，2，3： | 11 |
| 2 | Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material显示文摘Stem cell-based therapy has been used to treat ischaemic heart diseases for two decades.However,optimal cell types and transplantation methods remain unclear.This study evaluated the therapeutic effects of human umbilical cord mesenchymal stem cell(hUCMSC)sheet on myocardial infarction(MI).Methods:hUCMSCs expressing luciferase were generated by lentiviral transduction for in vivo bio-luminescent imaging tracking of cells.We applied a temperature-responsive cell culture surface-based method to form the hUCMSC sheet.Cell retention was evaluated using an in vivo bio-luminescent imaging tracking system.Unbiased transcriptional profiling of infarcted hearts and further immunohistochemical assessment of monocyte and macrophage subtypes were used to determine the mechanisms underlying the therapeutic effects of the hUCMSC sheet.Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function,angiogenesis and left ventricular remodelling.Results:When transplanted to the infarcted mouse hearts,hUCMSC sheet significantly improved the retention and survival compared with cell suspension.At the early stage of MI,hUCMSC sheet modulated inflammation by decreasing Mcp1-positive monocytes and CD68-positive macrophages and increasing Cx3cr1-positive non-classical macrophages,preserving the cardiomyocytes from acute injury.Moreover,the extracellular matrix produced by hUCMSC sheet then served as bioactive scaffold for the host cells to graft and generate new epicardial tissue,providing mechanical support and routes for revascularsation.These effects of hUCMSC sheet treatment significantly improved the cardiac function at days 7 and 28 post-MI.Conclusions:hUCMSC sheet formation dramatically improved the biological functions of hUCMSCs,mitigating adverse post-MI remodelling by modulating the inflammatory response and providing bioactive scaffold upon transplantation into the heart.Translational perspective:Due to its excellent availability as well as superior local cellular retention and survival,allogenic transplantation of hUCMSC sheets can more effectively acquire the biological functions of hUCMSCs,such as modulating inflammation and enhancing angiogenesis.Moreover,the hUCMSC sheet method allows the transfer of an intact extracellular matrix without introducing exogenous or synthetic biomaterial,further improving its clinical applicability. | Rui Guo Feng Wan Masatoshi Morimatsu Qing Xu Tian Feng Hang Yang Yichen Gong Shuhong Ma Yun Chang Siyao Zhang Youxu Jiang Heqing Wang Dehua Chang Hongjia Zhang Yunpeng Ling Feng Lan | 2021 | Bioactive Materials2021，6，9： | 6 |
| 3 | Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants显示文摘 | CAN‐FANGNIU WEIWEI QI‐YUNZHOU AI‐GUOTIAN YU‐JUNHAO WAN‐KEZHANG BIAOMA QINGLIN ZHENG‐BINZHANG JIN‐SONGZHANG SHOU‐YICHEN | 2012 | Plant Cell & Environment2012，，6： | 1 |
| 4 | Zingiber officinale (ginger) compounds have tetracycline‐resistance modifying effects against clinical extensively drug‐resistant Acinetobacter baumannii显示文摘 | Hui‐MinWang Chung‐YiChen Hsi‐AnChen Wan‐ChunHuang Wei‐RuLin Tun‐ChiehChen Chun‐YuLin Hsin‐JuChien Po‐LiangLu Chiu‐MeiLin Yen‐HsuChen | 2010 | Phytother Res2010，，12： | 1 |
| 5 | Cis9,trans11和trans10,cis12-CLA诱导乳腺癌细胞MCF-7凋亡机制的研究(英文)显示文摘Objective:The aim of the study was to explore the activities of cis9,trans11-CLA(C9,t11-CLA) and trans10,cis12-CLA(t10,c12-CLA) inhibiting tumor,and investigate their relationships with PPARγ and apoptotic proteins,and mechanism of anti-cancer.Methods:The inhibitory rate,cell growth curve and apoptotic morphological observation of MCF-7 cells were obtained by MTT assay,trypan blue staining and Hoechst33342 fluorescence staining.The apoptotic rate and cell cycle were detected with flow cytometry.Transcriptional level of genes was detected with RT-PCR semi-quantitative method,and Western blot was performed to detect proteins levels.Results:The two CLA isomers could reduce cell proliferation(P < 0.05),increase apoptotic rate(P < 0.05),and increase obviously the transcriptional and protein levels of PPARγ(P < 0.01).The synchronism and correlation between the effects of CLA to PPARγ and apoptotic proteins Bax,Bcl-2,Caspase 3 changes were found with the dose-and time-dependent manners.There was cooperative relation between the levels of PPARγ and the rates of Bax/Bcl-2,Caspase 3(small fragment) by experiments of PPARγ inhibitor GW9662 and ligand Rosiglitazone.Conclusion:The apoptotic pathway of PPARγ-Bcl-2-Caspase 3 signaling was found.The C9,t11-CLA and t10,c12-CLA could inhibit MCF-7 cell proliferation and promote apoptosis via activating PPARγ-Bcl-2-Caspase 3 pathway.CLA may be a kind of activator of PPARγ. | Xianzi Wan Xianlin Yuan Xiangling Yang Yichen Li Ling Zhong | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，10： | 0 |
| 6 | J-aggregation of photosensitizers leads to an ultrahigh drug-loading system for targeted delivery显示文摘Drug loading capacity is very important in the construction of targeted drug delivery systems(TDDSs)for the improvement of drug delivery efficiency.However,the drug-loading capacity of most nanomaterials is non-idealistic,and developing the high drug-loading TDDSs is still a critical challenge.In this work,an ultrahigh loading system(denoted as HMPB_(2))was prepared via J-aggregation of an aza-boron dipyrromethene derivative(Bod)by using hollow MnO_(2)modified with glucosamine pillar[5]arene as a carrier,which was demonstrated to have typical J-aggregate absorption of Bod,specific cancer cells targeting ability,negligible dark cytotoxicity,and potent phototoxicity.This work provides a successful example to construct an ultrahigh drug-loading system via J-aggregation for targeted delivery. | Yun Qu Wenjuan Jin Yichen Wan Zhichao Pei Yuxin Pei | 2024 | Chinese Chemical Letters2024，35，1： | 0 |
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