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| 1 | The first all-season sample set for mapping global land cover with Landsat-8 data显示文摘We report the world's first all-season training and validation sample sets for global land cover classification with Landsat-8 data.Prior to this,such samples were only available at a single date primarily from the growing season.It is unknown how much limitation such a single-date sample has to mapping global land cover in other seasons of the year.To answer this question,we selected available Landsat-8 images from four seasons and collected training and validation samples from them.We compared the performances of training samples in different seasons using Random Forest algorithm.We found that the use of training samples from any individual season would result in the best overall classification accuracy when validated by samples in the same season.The global overall accuracy from combined best seasonal results was 67.2% when classifying the 11 Level-1 classes in the Finer Resolution Observation and Monitoring of Global Land Cover(FROM-GLC) classification system.The use of training samples from all seasons(named all-season training sample set hereafter) produced an overall accuracy of 67.0%.We also tested classification within 10° latitude 60° longitude zones using all-season training subsample within each zone and obtained an overall accuracy of 70.2%.This indicates that properly grouped subsamples in space can help improve classification accuracies.All the results in this study seem to suggest that it is possible to use an all-season training sample set to reach global optimality with universal applicability in classifying images acquired at any time of a year for global land cover mapping. | Congcong Li Peng Gong Jie Wang Zhiliang Zhu Gregory S. Biging Cui Yuan Tengyun Hu Haiying Zhang Qi Wang Xuecao Li Xiaoxuan Liu Yidi Xu Jing Guo Caixia Liu Kwame O. Hackman Meinan Zhang Yuqi Cheng Le Yu Jun Yang Huabing Huang Nicholas Clinton | 2017 | Science Bulletin2017，62，7： | 21 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Annual 30-m land use/land cover maps of China for 1980–2015 from the integration of AVHRR, MODIS and Landsat data using the BFAST algorithm显示文摘Annual land use land cover(LULC)change information at medium spatial resolution(i.e.at 30 m)is required in numerous subjects,such as biophysical modelling,land management and global change studies.Annual LULC information,however,is usually not available at continental or national scale due to reasons such as insufficient remote sensing data coverage or lack of computational capabilities.Here we integrate high temporal resolution and coarse spatial resolution satellite images(i.e.,Moderate Resolution Imaging Spectroradiometer(MODIS)and Global Inventory Modelling and Mapping Studies(GIMMS)normalized difference vegetation index(NDVI))with high spatial resolution datasets(China’s Land-Use/cover Datasets(CLUDs)derived from 30-meter Landsat TM/ETM+/OLI)to generate reliable annual nominal 30 m LULC maps for the whole of China between 1980 and 2015.We also test the performance of a statistical based change detection algorithm(Breaks for Additive Seasonal and Trend),originally designed for tracking forest change,in classifying all-type LULC change.As a result,a nominal 30 m annual land use/land cover datasets(CLUD-A)from 1980 to 2015 was developed for the whole China.The mapping results were assessed with a change sample dataset,a regional annual validation sample set and a three-year China sample set.Of the detected change years,75.61%matched the exact time of conversion within±1 year.Annual mapping results provided a detail process of urbanization,deforestation,afforestation,water and cropland dynamics over the past 36 years.The consistent characterization of land change dynamics for China can be further used in scientific research and to support land management for policy-makers. | Yidi XU Le YU Dailiang PENG Jiyao ZHAO Yuqi CHENG Xiaoxuan LIU Wei LI Ran MENG Xinliang XU Peng GONG | 2020 | Science China Earth Sciences2020，63，9： | 12 |
| 4 | Inlet Recirculation Influence to the Flow Structure of Centrifugal Impeller显示文摘Inlet recirculation is proved as an effective way for centrifugal compressor surge margin extension,and is successively used in some engineering applications.Unfortunately its working mechanism is still not being well understood,which leads to redesigning of inlet recirculation mostly by experience.Also,most study about inlet recirculation is steady to date.It is necessary to study surge margin extension mechanism about inlet recirculation.To expose the mechanism in detail,steady and unsteady numerical simulations were performed on a centrifugal compressor with and without inlet recirculation.The results showed that,with inlet recirculation,the inlet axial velocity is augmented,relative Mach number around blade tip leading edge area is significantly reduced and so is the flow angle.As the flow angle decreased,the incidence angle reduced which greatly improves the flow field inside the impeller.Moreover,inlet recirculation changes the blade loading around blade tip and restrains the flow separation on the blade suction side at the leading edge area.The unsteady results of static pressure around blade surface,entropy at inlet crossflow section and vorticity distributions at near tip span surface indicated that,at near stall condition,strong fluctuation exists in the vicinity of tip area due to the interaction between tip leakage flow and core flow.By inlet recirculation these strong flow fluctuations are eliminated so the flow stability is greatly enhanced.All these improvements mentioned above are the reason for inlet recirculation delays compressor stall.This research reveals the surge margin extension reason of inlet recirculation from an unsteady flow viewpoint and provides important reference for inlet recirculation structure design. | YANG Ce CHEN Shan LI Du YANG Changmao WANG Yidi | 2010 | Chinese Journal of Mechanical Engineering2010，23，5： | 10 |
| 5 | High-energy cathode materials for Li-ion batteries: A review of recent developments显示文摘Lithium ion batteries(LIBs) represent one of the most promising solutions for environmentally friendly transportation such as electric vehicles. The demand for high energy density, low cost and environmentally friendly batteries makes high-capacity cathode materials very attractive for future LIBs. Layered Li NixCoyMnzO2(x+y+z=1), Li-rich oxides and Li-V-O compounds have attracted much attention due to their high capacities in recent years. In this review, we focus on the state-of-the-art research activities related to Li NixCoyMnzO2, Li-rich oxides and Li-V-O compounds, including their structures, reaction mechanisms during cycling, challenges and strategies that have been studied to improve their electrochemical performances. | ZHANG YiDi LI Yi XIA XinHui WANG XiuLi GU ChangDong TU JiangPing | 2015 | Science China(Technological Sciences)2015，58，11： | 7 |
| 6 | Quality control of Semen Ziziphi Spinosae standard decoction based on determination of multi-components using TOF-MS/MS and UPLC-PDA technology显示文摘A sensitive,fast and comprehensive method for the quality assessment of Semen Ziziphi Spinosae(SZS)standard decoction with characterization of its chemical components was developed and validated.UPLC-Q/TOF-MS/MS system was used to identify thirty-six chemical components of SZS standard decoction which included nucleosides,phenolic acids,alkaloids,and flavonoids.Furthermore,a UPLC-PDA method was validated to simultaneously determine adenosine,protocatechuic acid,magnoflorine,catechin,protocatechin,vicenin II,spinosin,kaempferol-3-rutinoside,and 6'''-feruloylspinosin which represent four species of characteristic compounds.The qualitative method had been validated according to Chinese Pharmacopoeia(2015 edition)in terms of lineary,repeatability,recovery and stability for all analytes,with the results showing good precision,accuracy and stability.In conclusion,the method using UPLC combined with MS and PDA provided a novel way for the standardization and identification of SZS standard decoction,and also offered a basis for qualitative analysis and quality assessment of the preparations for SZS standard decoction. | Di Wang Qing Li Ran Liu Huarong Xu Yidi Yin Yifan Wang Huijia Wang Kaishun Bi | 2019 | Journal of Pharmaceutical Analysis2019，9，6： | 6 |
| 7 | Highly efficient base editing in human tripronuclear zygotes显示文摘 | Changyang Zhou Meiling Zhang Yu Wei Yidi Sun Yun Sun Hong Pan Ning Yao Wanxia Zhong Yixue Li Weiping Li3 Hui Yang Zi-jiang Chen | 2017 | Protein & Cell2017，8，10： | 6 |
| 8 | Robust minimum-variance time-reversal beamforming for target detection and localization in uncertain environment显示文摘在有颠倒( TR )技术是 investigated.TR 的柔韧的时间的不明确的环境的目标察觉和本地化是物理进程以及信号 processing.Therefore 的一个方法,经由 TR 执行 beamforming 是自然的,即, TR beamforming ( TRBF ) .To 还原剂 TR 上的环境无常的效果时间空间的集中,播送 TRBF 与当模特儿而不是物理探查来源被学习,有斜装载的柔韧的最小变化的收到 TRBF 被放 forward. | RUAN Yidi PAN Xiang LI Jianlong GONG Xianyi | 2010 | Chinese Journal of Acoustics2010，29，1： | 3 |
| 9 | Rabies virus co-localizes with early(Rab5) and late(Rab7) endosomal proteins in neuronal and SH-SY5Y cells显示文摘Rabies virus(RABV) is a highly neurotropic virus that follows clathrin-mediated endocytosis and p H-dependent pathway for trafficking and invasion into endothelial cells. Early(Rab5, EEA1) and late(Rab7, LAMP1) endosomal proteins play critical roles in endosomal sorting, maturity and targeting various molecular cargoes, but their precise functions in the early stage of RABV neuronal infection remain elusive. In this study, the relationship between enigmatic entry of RABV with these endosomal proteins into neuronal and SH-SY5 Y cells was investigated.Immunofluorescence, TCID_(50) titers, electron microscopy and western blotting were carried out to determine the molecular interaction of the nucleoprotein(N) of RABV with early or late endosomal proteins in these cell lines. The expression of N was also determined by down-regulating Rab5 and Rab7 in both cell lines through RNA interference. The results were indicative that N proficiently colocalized with Rab5/EEA1 and Rab7/LAMP1 in both cell lines at 24 and 48 h post-infection, while N titers significantly decreased in early infection of RABV. Down-regulation of Rab5 and Rab7 did not inhibit N expression, but it prevented productive infection via blocking the normal trafficking of RABV in a low pH environment. Ultrathin sections of cells studied by electron microscope also verified the close association of RABV with Rab5 and Rab7 in neurons. From the data it was concluded that primary entry of RABV strongly correlates with the kinetics of Rab-proteins present on early and late vesicles, which provides helpful clues to explain the early events of RABV in nerve cells. | Waqas Ahmad Yingying Li Yidi Guo Xinyu Wang Ming Duan Zhenhong Guan Zengshan Liu Maolin Zhang | 2017 | Virologica Sinica2017，32，3： | 3 |
| 10 | Ten years after Hurricane Katrina: monitoring recovery in New Orleans and the surrounding areas using remote sensing显示文摘Remote sensing data have been widely used in pre-hazard prevention/preparation, emergency response and post-hazard recovery monitoring. Hurricane Katrina caused serious damage to the environment, society and economy in the southern United States in 2005. On the 10 th anniversary of Hurricane Katrina, we monitored the recovery process in New Orleans and the surrounding area based on remote sensing. Results from multi-source remote sensing data indicated that the average vegetation conditions of the affected areas have not fully recovered compared with the pre-disaster conditions, especially in the hurricane's landfall area. Analysis from moderate resolution Landsat data showed that many civil engineering works have been undertaken in the city of New Orleans to prevent future disasters. Frequent observation using highresolution images recorded the progress of some of these civil construction projects(e.g. the 17 th Street Canal pumping station) in New Orleans. In this case study, we illustrated the capabilities of remote sensing techniques in recovery monitoring following a natural disaster. International/institutional cooperation is suggested to improve Earth observation capability in hazard monitoring. More Chinese Earth observation data are expected to be used in international monitoring. | Xuecao Li Le Yu Yidi Xu Jun Yang Peng Gong | 2016 | Science Bulletin2016，61，18： | 2 |
| 11 | Generation of a Golden Leaf Triploid Poplar by Repressing the Expression of GLK Genes显示文摘Poplar trees are excellent varieties widely used for gardening and greening.However,their single color and floating fluffy seeds are major disadvantages.Plant species or varieties with variegated leaves are desperately needed to meet various demands for gardens,urban greening and landscape decoration,as they produce rich foliage colors that are aesthetically pleasing and functional.In this study,we generated a golden leaf triploid poplar(P.alba×P.berlinensis)by repressing the expression of GLK(Golden2-like)genes in leaves.The triploid golden leaf poplar had reduced chlorophyll content but almost no change in the growth rate.It has great potential in landscaping once it passes safety assessments. | Yidi Li Chenrui Gu Huixin Gang Yu Zheng Guifeng Liu Jing Jiang | 2021 | Forestry Research2021，1，1： | 1 |
| 12 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 13 | Kinase-substrate Edge Biomarkers Provide a More Accurate Prognostic Prediction in ER-negative Breast Cancer显示文摘The estrogen receptor(ER)-negative breast cancer subtype is aggressive with few treatment options available.To identify specific prognostic factors for ER-negative breast cancer,this study included 705,729 and 1034 breast invasive cancer patients from the Surveillance,Epidemiology,and End Results(SEER)and The Cancer Genome Atlas(TCGA)databases,respectively.To identify key differential kinase-substrate node and edge biomarkers between ER-negative and ERpositive breast cancer patients,we adopted a network-based method using correlation coefficients between molecular pairs in the kinase regulatory network.Integrated analysis of the clinical and molecular data revealed the significant prognostic power of kinase-substrate node and edge features for both subtypes of breast cancer.Two promising kinase-substrate edge features,CSNK1A1-NFATC3 and SRC-OCLN,were identified for more accurate prognostic prediction in ERnegative breast cancer patients. | Yidi Sun Chen Li Shichao Pang Qianlan Yao Luonan Chen Yixue Li Rong Zeng | 2020 | Genomics, Proteomics & Bioinformatics2020，18，5： | 1 |
| 14 | Efficient removal and recovery of Cd^(2+)from aqueous solutions by capacitive deionization(CDI)method using biochars显示文摘Biochars are low-cost and sustainable materials for environmental technologies.In this work,we pre-pared three biochars using pomelo peel(P-BC),algae(A-BC),and corncob(C-BC)to recover Cd^(2+)from wastewater via capacitive deionisation(CDI).A-BC possesses the highest amount of mesopores and nitro-gen functionality and attains the highest removal of Cd^(2+)via physical adsorption.For the electro-sorption capacity,C-BC and A-BC perform better capacitive removal of Cd^(2+)than P-BC due to the smaller charge-transfer and mass-transfer resistances.Also,this work investigated the impacts of surface morphology,cell voltage,NaCl,initial pH,and Cd^(2+)concentrations on Cd^(2+)capacitive removal and electrode regen-eration performances.The results indicated that A-BC and C-BC may be prospective materials for Cd^(2+)removal from wastewater by CDI.However,the presence of competing cations at high concentrations may influence the removal of Cd^(2+)at a low level,requiring the modification of A-BC and C-BC in future work. | Zhao Song Lingyu Li Yidi Chen Xiaoguang Duan Nanqi Ren | 2023 | Journal of Materials Science & Technology2023，，17： | 1 |
| 15 | A UFLC/MS/MS method for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale (Sam.) Juz. in rat plasma显示文摘A sensitive and reliable ultra fast liquid chromatography tandem mass spectrometry(UFLC-MS/MS)method has been developed and validated for simultaneous quantitation of alisol A and alisol B 23-acetate from Alisma orientale(Sam.)Juz.in rat plasma using diazepam as an internal standard(IS).The plasma samples were extracted by liquideliquid extraction with methyl tert-butyl ether and separated on a Venusil MP C18 column(100 mm×2.1 mm,3.0 mm)(Venusil,China)using gradient elution with the mobile phase consisting of methanol and 0.1%acetic acid in water at a flow rate of 0.4 ml/min.The two analytes were monitored with positive electrospray ionization by multiple reaction monitoring mode(MRM).The lower limit of quantitation was 5.00 ng/ml for alisol A and 5.00 ng/ml for alisol B 23-acetate.The calibration curves were linear in the range of 5.00 e2500 ng/ml for alisol A and 5e2500 ng/ml for alisol B 23-acetate.The mean extraction recoveries were above 63.8%for alisol A and 68.0%for alisol B 23-acetate from biological matrixes.Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria(15%).The validated method was successfully applied to the pharmacokinetic study of alisol A and alisol B 23-acetate in rat plasma after oral administration of alcohol extract of Alismatis Rhizoma. | Yaowen Zhang Qing Li Chunxiao Lv Yidi Yin Kaishun Bi | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，5： | 1 |
| 16 | In situ formation of lithiophilic Li_(22)Sn_(5) alloy and high Li-ion conductive Li_(2)S/Li_(2)Se via metal chalcogenide SnSSe for dendrite-free Li metal anodes显示文摘Lithium metal has gained extensive attention as the most ideal candidate for next-generation battery anode owing to the ultrahigh specific capacity and the lowest electrochemical potential.However,uncontrollable dendrite growth and huge volume variation extremely restrict the future deployment of lithium metal batteries.Herein,we report metal chalcogenide SnSSe with unique nanoplate stacking structure as a robust substrate for stable Li metal anode.During the initial Li plating process,lithiophilic Li_(22)Sn_(5) alloy and Li_(2)S/Li_(2)Se sites are obtained via in-situ electrochemical reaction of Li metal and SnSSe.Density functional theory(DFT)calculation demonstrates that the formed Li_(2)S/Li_(2)Se achieves low Li diffusion energy barrier,ensuring rapid Li~+migration.Li_(22)Sn_(5) alloy provides strong nucleation sites,promoting uniform Li nucleation.Furthermore,in-situ optical microscopy analysis suggests that the synthesized effect fundamentally inhibits lithium dendrite growth.Consequently,SnSSe modified Cu foil delivered an ultralow nucleation overpotential,superior cycling stability with 450 cycles(Coulombic efficiency,>98%),and excellent plating/stripping behavior over 2200 h at 0.5 mA cm^(-2).Moreover,the brilliant reversible cycles and rate capability were also realized in Li@SnSSe//LiFePO_(4)(LFP)full cell,shedding light on the feasibility of SnSSe for stable and dendrite-free lithium metal anode. | Yaya Wang Yang Guo Jiang Zhong Meng Wang Lei Wang Shengyang Li Song Chen Hongli Deng Yong Liu Yidi Wu Jian Zhu Bingan Lu | 2022 | Journal of Energy Chemistry2022，31，10： | 0 |
| 17 | Application of nitrogen loaded biochar in purifying agricultural wastewater and as a nitrogen releaser for rice production显示文摘Herein a new approach to the application of agricultural eutrophic wastewater for rice plant cultivation is described.Biochar was used as a medium for the sorption of ammonia from simulated wastewater and subsequently as a nitrogen(N)releaser in the cultivation of rice plants.The main goals of this approach were to isolate ammonia from simulated wastewater and transfer it into rice cultivation,and or explore how exogenous N promoted the growth of rice.The results demonstrate that according to X-ray diffraction phase analysis,most of the properties of biochar were retained before and after loading NH_(4)^(+)-N.Compared with biochar,the crystal peak of AlOOH in N-loaded biochar(NLB)disappeared and the intensity of the crystal peak of CuCaSe_(2)decreased,which was the important mechanism allowing it to adsorb 30.8%of the N present in simulated low N-concentration agricultural wastewater.The soil N content in NLB treatments was higher than in Non-NLB treatments during the critical tillering and reproductive growth stages.Moreover,the N adsorption-desorption process of NLB matched the N requirements of the rice plant,and thus greatly increased the tiller number by 11.9%and rice yield by 7.5%.These results indicated that the indirect use of ammonia derived from wastewater using biochar as a sorption and releasing medium for rice plant cultivation was promising.This is the first time that biochar was used for possibly indirect reuse of agricultural eutrophic wastewater and enhancement of rice plant growth. | Hongyang Chen Yidi Sun Yang Sun Yanzhi Wang Yanqi Li Qi Wu Daocai Chi | 2023 | International Journal of Agricultural and Biological Engineering2023，16，4： | 0 |
| 18 | Audio-visual keyword transformer for unconstrained sentence-level keyword spotting显示文摘As one of the most effective methods to improve the accuracy and robustness of speech tasks,the audio-visual fusion approach has recently been introduced into the field of Keyword Spotting(KWS).However,existing audio-visual keyword spotting models are limited to detecting isolated words,while keyword spotting for unconstrained speech is still a challenging problem.To this end,an Audio-Visual Keyword Transformer(AVKT)network is proposed to spot keywords in unconstrained video clips.The authors present a transformer classifier with learnable CLS tokens to extract distinctive keyword features from the variable-length audio and visual inputs.The outputs of audio and visual branches are combined in a decision fusion module.As humans can easily notice whether a keyword appears in a sentence or not,our AVKT network can detect whether a video clip with a spoken sentence contains a pre-specified keyword.Moreover,the position of the keyword is localised in the attention map without additional position labels.Exper-imental results on the LRS2-KWS dataset and our newly collected PKU-KWS dataset show that the accuracy of AVKT exceeded 99%in clean scenes and 85%in extremely noisy conditions.The code is available at https://github.com/jialeren/AVKT. | Yidi Li Jiale Ren Yawei Wang Guoquan Wang Xia Li Hong Liu | 2024 | CAAI Transactions on Intelligence Technology2024，9，1： | 0 |
| 19 | Unraveling the skatole biodegradation process in an enrichment consortium using integrated omics and culture-dependent strategies显示文摘3-Methylindole(skatole)is regarded as one of the most offensive compounds in odor emission.Biodegradation is feasible for skatole removal but the functional species and genes responsible for skatole degradation remain enigmatic.In this study,an efficient aerobic skatole-degrading consortium was obtained.Rhodococcus and Pseudomonas were identified as the two major and active populations by integrated metagenomic and metatranscriptomic analyses.Bioinformatic analyses indicated that the skatole downstream degradation wasmainly via the catechol pathway,and upstream degradation was likely catalyzed by the aromatic ring-hydroxylating oxygenase and flavin monooxygenase.Genome binning and gene analyses indicated that Pseudomonas,Pseudoclavibacter,and Raineyella should cooperate with Rhodococcus for the skatole degradation process.Moreover,a pure strain Rhodococcus sp.DMU1 was successfully obtained which could utilize skatole as the sole carbon source.Complete genome sequencing showed that strain DMU1 was the predominant population in the consortium.Further crude enzyme and RT-qPCR assays indicated that strain DMU1 degraded skatole through the catechol ortho-cleavage pathway.Collectively,our results suggested that synergistic degradation of skatole in the consortium should be performed by diverse bacteria with Rhodococcus as the primary degrader,and the degradation mainly proceeded via the catechol pathway. | Qiao Ma Nan Meng Jiancheng Su Yujie Li Jiazheng Gu Yidi Wang Jingwei Wang Yuanyuan Qu Zelong Zhao Yeqing Sun | 2023 | Journal of Environmental Sciences2023，，5： | 0 |
| 20 | Long-term follow-up of volumetric modulated arc therapy in definitive radiotherapy for cervical cancer: A single-center retrospective experience显示文摘Objective:To evaluate the treatment outcomes and toxicity of volumetric modulated arc therapy(VMAT)in definitive radiotherapy for patients with cervical cancer.Methods:Medical records of 440 patients with cervical cancer treated with VMAT in definitive radiotherapy with or without concurrent chemotherapy between January 2010 and June 2015 in our institution were retrospectively reviewed.A dose of 45 Gy was prescribed to the planning target volume with VMAT in 25 fractions,followed by high-dose rate intracavitary brachytherapy with 28–36 Gy in 4–6 fractions.Survival time was estimated using Kaplan-Meier method.Risk factors for treatment failure were identified using Cox proportional hazards regression model.Results:The median follow-up periods were 58.3 months(range:2.7–112.9 months).The 3-and 5-year survival rates were overall survival(OS)79.4%and 74.4%,cancer-specific survival(CSS)81.4%and 77.1%,progressionfree survival(PFS)73.3%and 71.1%,locoregional control(LC)83.3%and 82%and distant metastasis-free survival(DMFS)80.8%and 77.7%,respectively.The 5-year OS for patients with different stages was IB191.6%,IB2100%,IIA 82.5%,IIB 74.4%,IIIA 57.1%,IIIB 61.7%and IVB 17.6%,respectively.Of the 124 patients with recurrence,93 patients(75.0%)experienced distant failure.Multivariate analysis showed that non-squamous cell carcinoma of the cervix(P<0.001),more advanced stage(P=0.004),pelvic lymph node metastasis(P<0.001)and para-aortic lymph node metastasis(P=0.019)were all independent risk factors of PFS.The incidence of grades 3–4 late bowel toxicities was 2.7%.No grades 3–4 bladder toxicity was observed.Conclusion:This study demonstrated that the use of VMAT in definitive radiotherapy resulted in satisfactory outcomes with acceptable toxicities for patients with cervical cancer.Distant metastasis predominated as the pattern of disease relapse.Further studies are needed to investigate the intensified systemic therapies. | Yidi Yuan Jing You Weihu Wang Xiaofan Li | 2020 | Radiation Medicine and Protection2020，1，2： | 0 |
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