|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | RBCC引射模态发动机设计技术研究进展显示文摘RBCC发动机未来可实现地面自主水平起降、可重复使用等功能,具有潜在的低成本和高可靠性优势,因而成为天地往返推进系统备选方案之一。但引射模态发动机性能偏低,限制了RBCC发动机的应用,引射模态发动机性能亟需提升。针对多模态RBCC引射模态发动机性能偏低的问题,阐述了RBCC引射模态发动机基本气动热力过程。综述了引射模态推力增益、主次流相互作用以及二次燃烧组织等引射模态发动机关键技术的研究进展,并分析了RBCC引射模态发动机设计进一步的研究方向。 | 林彬彬 Yidi Tang 尚永锋 张恒铭 | 2020 | 飞航导弹2020，，2： | 2 |
| 3 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 4 | On‐device audio‐visual multi‐person wake word spotting显示文摘Audio‐visual wake word spotting is a challenging multi‐modal task that exploits visual information of lip motion patterns to supplement acoustic speech to improve overall detection performance.However,most audio‐visual wake word spotting models are only suitable for simple single‐speaker scenarios and require high computational complexity.Further development is hindered by complex multi‐person scenarios and computational limitations in mobile environments.In this paper,a novel audio‐visual model is proposed for on‐device multi‐person wake word spotting.Firstly,an attention‐based audio‐visual voice activity detection module is presented,which generates an attention score matrix of audio and visual representations to derive active speaker representation.Secondly,the knowledge distillation method is introduced to transfer knowledge from the large model to the on‐device model to control the size of our model.Moreover,a new audio‐visual dataset,PKU‐KWS,is collected for sentence‐level multi‐person wake word spotting.Experimental results on the PKU‐KWS dataset show that this approach outperforms the previous state‐of‐the‐art methods. | Yidi Li Guoquan Wang Zhan Chen Hao Tang Hong Liu | 2023 | CAAI Transactions on Intelligence Technology2023，8，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员