| 1 | Effect of cluster needling at scalp acupoints on differential protein expression in rat brain tissue after acute focal cerebral ischemia显示文摘Objective:To explore the function of cluster needling at scalp points therapy on regulating differential protein's expression at different time points in middle cerebral artery occlusion(MCAO)model rats.Methods:Fifty-four rats were divided into three groups randomly and 18 rats in each group.The groups respectively were the model group(group M,n=18),cluster needling at scalp points group(group C,n=18),false operation group(group F,n=18).Each group was then assigned in three subgroups,including 24-h,7-day,and 14-day subgroups.Six rats in each subgroup.Acupuncture at Baihui(GV20)and 2 points beside Baihui,which was 3 e4 mm away from the midline.Longa score was used to evaluated neurological effects.Proteomics methods were used to identify differentially expression proteins with a standard of fold change greater than 1.5 and P<.05 at different times.Results:1.Nerve function scoring:The nerve function scores at 7 and 14 days decreased in group C,which showed better neural function than group M(P<.05).2.Fold change in proteins:Group M showed932 differentially expressed proteins compared with group F,and among them,414 proteins showed significant changes in expression after acupuncture.The expression levels of Cdc42 and GFAP were increased,and Mag,Shank2,and MBP levels were decreased.In the Gene Ontology analysis,the cellular component consisted of the terms cytoplasm,cytoskeleton,lysosome,and plasma membrane.The main related biological processes were cellecell signaling,protein transport,aging,and cell adhesion.Many synaptic and metabolic pathways were found by KEGG analysis.Conclusion:Cluster needling at scalp acupoints can improve the nerve function score and improve dyskinesia in MCAO model rats.Cluster needling at scalp acupoints can regulate the expression of 414 proteins,including Cdc42,GFAP,Mag,Shank2,and MBP,which are related to cerebral ischemia.The differential proteins are major concentration in cytoplasm,cytoskeleton,lysosomes,and plasma membrane,participate in cellecell signaling,protein transport,aging,and cell adhesion,and act through multiple synaptic and metabolic pathways to exert their biological functions. | Xiaona Wu Jinxia Ni Huiyan An Yintong Gao Miaomiao Li Zhenzhen Huang Jingni Xu | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，3： | 1 |
| 2 | On the excavation-induced stress drop in damaged coal considering a coupled yield and failure criterion显示文摘Investigating the stress drop of abutment pressure is the key to a deep quantitative analysis of the discontinuous stress redistribution under mining.In the present study,uniaxial and triaxial compression tests are carried out separately to determine the bulk and shear moduli,the cohesion,and the internal friction angle of the coal samples.By extending the meaning of Mohr’s circle referring to yield stress instead of the maximum principal stress,a yield line is introduced to illustrate the stress drop of Mohr’s circle referring to yield stress instead of the maximum principal stress at the elastoplastic boundary.Furthermore,a theoretical solution of the stress drop as a function of the damage is proposed to investigate how the abutment pressure differs considering the yield line and failure line.In addition,applying the stress drop at the yield line in non-pillar mining,top coal mining,and protective coal mining shows that the damage has a nonlinearly positive influence on the stress drop.The results shows that the bulk modulus and internal friction angle have a more sensitive influence on the stress drop than do the shear modulus and cohesion.Finally,the stress drop is divided into a discontinuous stress drop at the yield line and a structural stress drop at the failure line.The stress drop is effective in describing the discontinuous stress redistribution and shows a clear difference in the movement direction of Mohr’s circle considering the unloading pressure. | Dongjie Xue Jie Zhou Yintong Liu Lie Gao | 2020 | International Journal of Coal Science & Technology2020，7，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员