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| 1 | Quantifying 3D cell-matrix interactions during mitosis and the effect of anticancer drugs on the interactions显示文摘The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all these processes and thus an in-depth understanding of forces during mitosis gains insight into disease diagnosis and disease treatment. Here, we develop a traction force microscope method based on monolayer fluorescent beads for measuring the weak traction force (tens of Pa) of mitotic cells in three dimensions. We quantify traction forces of human ovarian granulosa (KGN) cells exerted on the extracellular matrix throughout the entire cell cycle in three dimensions. Our measurements reveal how forces vary during the cell cycle, especially during cell division. Furthermore, we study the effect of paclitaxel (PTX) and nocodazole (NDZ) on mitotic KGN cells through the measurement of traction forces. Our results show that mitotic cells with high concentrations of PTX exert a larger force than those with high concentrations of NDZ, which proved to be caused by changes in the structure and number of microtubules. These findings reveal the key functions of microtubule in generating traction forces during cell mitosis and explain how dividing cells regulate themselves in response to anti-mitosis drugs. This work provides a powerful tool for investigating cell-matrix interactions during mitosis and may offer a potential way to new therapies for cancer. | Yongman Liu Jianye Wang Yong Su Xiaohai Xu Hong Liu Kainan Mei Shihai Lan Shubo Zhang Xiaoping Wu Yunxia Cao Qingchuan Zhang Shangquan Wu | 2021 | Nano Research2021，14，11： | 3 |
| 2 | Typical Causes and Countermeasures of Moisture Damage for Asphalt Pavement 显示文摘 | Yang Ruochong Liang Xisan Lai Yongman | 2008 | Journal of Tongfi University (Natural Science)2008，6，： | 1 |
| 3 | Ordered Meso- porous Porphyrinic Carbons with Very High Electrocatalytic Activity for the Oxygen Reduction Reaction 显示文摘 | JAE Y TAEYOUNG K YONGMAN C | 2013 | Scientific Report2013，3，2715： | 1 |
| 4 | A study by in situ techniques of the thermal evolution of the structure显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engineering Science2002，57，： | 1 |
| 5 | A study by in situ techniques of evolution of the structure显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engineering Science2002，57，： | 1 |
| 6 | Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions显示文摘 | Yongman I W Ward O P | 1991 | Applied and Environ- mental Microbiology1991，57，2： | 1 |
| 7 | A study by in situ techniques of the thermal evolution of the structure of a Mg-Al-CO3 layered double hydroxide显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engineering Science2002，57，： | 1 |
| 8 | A study by in situ techniques of the thermal evolution of the structure 显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engineering Science2002，57，： | 1 |
| 9 | MPEG-7 Homogeneous Texture Descriptor显示文摘 | Ro Yongman Kim M Ho Kyung Kang | 2001 | ETRI Journal2001，23，2： | 1 |
| 10 | The study on heat transfer characteristic of coil's internal-melt ice stor- age tank for elliptical tube 显示文摘 | GUAN Wayout LI Lifu LIN Yongman | 2012 | International Journal of Engineering and Industries2012，3，2： | 1 |
| 11 | A study by in situ techniques of evolution of the structure显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engineering Science2002，57，： | 1 |
| 12 | Meta-Analysis: The Efficacy and Safety of Paricalcitol for the Treatment of Secondary Hyperparathyroidism and Proteinuria in Chronic Kidney Disease显示文摘 | Tianzhao Han Gong Rong Dayong Quan Ying Shu Zhu Liang Ninglan She Manli Liu Bing Yang Gong Cheng Yongman Lv Leonard Stern Sihe Wang | 2012 | BioMed Research International2012，，： | 1 |
| 13 | A study by in situ techniques of the thermal evolution of the structure of a Mg-Al-CO3 layered double hydroxide显示文摘 | Weishen Yang Yongman Kim Paul K T Liu | 2002 | Chemical Engi-neering Science2002，57，： | 1 |
| 14 | A study by in situ techniques of the thermal evolution of the structure of a Mg-Al-CO3 layered double hydroxide显示文摘 | Yang Weishen Kim Yongman Liu Paul K.T | | 0，，15： | 1 |
| 15 | Animal models for anti-neutrophil cytoplasmic antibody-associated vasculitis:Are current models good enough?显示文摘Antineutrophil cytoplasmic autoantibody(ANCA)-associated vasculitis(AAV)is a rare and severe systemic autoimmune disease characterized by pauci-immune necrotizing inflammation of small blood vessels.AAV involves multiple organ systems throughout the body.Our knowledge of the pathogenesis of AAV has increased considerably in recent years,involving cellular,molecular and genetic factors.Because of the controlled environment with no other confounding factors,animal models are beneficial for studying the mechanistic details of disease development and for providing novel therapeutic targets with fewer toxic side effects.However,the complexity and heterogeneity of AAV make it very difficult to establish a single animal model that can fully represent the entire clinical spectrum found in patients.The aim of this review is to overview the current status of animal models for AAV,outline the pros and cons of methods,and propose potential directions for future research. | Fei Huang Yongman Lv Siyang Liu Hao Wu Qingquan Liu | 2023 | Animal Models and Experimental Medicine2023，6，5： | 0 |
| 16 | Dynamic response of the cell traction force to osmotic shock显示文摘Osmotic pressure is vital to many physiological activities,such as cell proliferation,wound healing and disease treatment.However,how cells interact with the extracellular matrix(ECM)when subjected to osmotic shock remains unclear.Here,we visualize the mechanical interactions between cells and the ECM during osmotic shock by quantifying the dynamic evolution of the cell traction force.We show that both hypertonic and hypotonic shocks induce continuous and large changes in cell traction force.Moreover,the traction force varies with cell volume:the traction force increases as cells shrink and decreases as cells swell.However,the direction of the traction force is independent of cell volume changes and is always toward the center of the cell-substrate interface.Furthermore,we reveal a mechanical mechanism in which the change in cortical tension caused by osmotic shock leads to the variation in traction force,which suggests a simple method for measuring changes in cell cortical tension.These findings provide new insights into the mechanical force response of cells to the external environment and may provide a deeper understanding of how the ECM regulates cell structure and function. | Yongman Liu Wenjie Wu Shuo Feng Ye Chen Xiaoping Wu Qingchuan Zhang Shangquan Wu | 2023 | Microsystems & Nanoengineering2023，9，5： | 0 |
| 17 | Allomaternal care and‘adoption'in an edge-of-range population of Taihangshan macaques in Northern China显示文摘Among mammals,mothers provide maternal care and protection starting from gestation and they ensure nutritional supply via lactation after birth(Clutton-Brock 1991).However,in social mammals,infants may also be cared for by a variety of other group members,such as fathers,siblings,a host of more distant relatives,and even unrelated individuals.Care by individuals other than the mother is known as allomaternal care(nonmaternal care)(Ishizuka 2020).Moreover,adoption of an orphaned infant is a special case of allomaternal care(Chaves et al.2020). | Yongman Guo Cyril C Grueter Jiqi Lu | 2023 | Current Zoology2023，69，2： | 0 |
| 18 | Therapeutic DC vaccination with IL-2 as a consolidation therapy for ovarian cancer patients: a phase Ⅰ/Ⅱ trial显示文摘当卵巢的癌症(OvCa ) 对外科和常规化疗作出回应很好时,先进 OvCa 的高复发率被观察。在这阶段 I/II 研究,有最小的剩余疾病的 10 个 OvCa 病人与自体同源的树枝状的房间(DC ) 和 IL-2 被对待评估这治疗学的策略的安全和可行性并且描绘通过这治疗导致的抗原特定的有免疫力的改变。在起始的 debulking 和化疗以后的约 4 个月,病人们收到了自体同源的导出单核白血球的 DC 的二下的剂量在 4 星期的间隔与自体同源的肿瘤 lysate 和锁眼恋栈者 hemocyanin (KLH ) 搏动了。在每 DC 接种以后,低剂量(200 mIU ) IL-2 作为一个有免疫力的助手被介绍 14 连续的天。种痘很好被容忍。没有疾病恶化,在三,从 10 个病人,,在起始的治疗以后的包括地位在 DC 种痘以后显示出完全的宽恕(CR ) 的维护 83, 80.9 和 38.2 个月。有稳定的疾病(SD ) 的一个病人在 DC 种痘以后经历了肿瘤的完全的消失,并且这地位被维持 50.8 个月直到肿瘤复发。在二,有部分反应(PR ) 的病人不正在回答种痘和他们的疾病复发了到 DC。在有疾病的三个病人免费长期的幸存,重要有免疫力的改变被观察,包括的增加的生来的杀手(NK ) 活动, IFN-γ在 DC 种痘以后的 -secreting T 房间, immune-stimulatory cytokine 分泌物和减少的免疫者镇压的因素分泌物。在有 NED 地位和增加的全面幸存的病人,因此, DC 种痘导致了肿瘤相关的免疫,潜在地对 OvCa 与长期的临床的回答联系了。 | Soyoung Baek Yong-Man Kim Sung-Bae Kim Choung-Soo Kim Seog-Woon Kwon YongMan Kim HyunSoo Kim Hyunah Lee | 2015 | Cellular & Molecular Immunology2015，12，1： | 0 |
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