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| 1 | Adsorption of Cd(Ⅱ) from acidic aqueous solutions by tourmaline as a novel material显示文摘Batch experiments were conducted to investigate the behavior and mechanisms for the adsorption of Cd(Ⅱ) from aqueous solutions by tourmaline under acidic conditions. The results indicated that the adsorption of Cd(Ⅱ) significantly depend on the adsorption time, temperature, and the initial concentration of the metal ion. Furthermore, tourmaline had a very good adsorption capacity for Cd(Ⅱ) in acidic, neutral and alkaline aqueous solutions. This good adsorption capacity is attributed to the observation that tourmaline can automatically adjust the pH values of acidic (except pH 2.0 and 3.0), neutral or alkaline aqueous solutions to 6.4. Specifically, the removal capacity for Cd(Ⅱ) was higher at strongly acidic pH values (in contrast to industrial wastewater pH values) compared to that obtained for other types of adsorbents. Furthermore, the results obtained in this study showed good fits to the Langmuir and Freundlich adsorption isotherms. However, the Langmuir model fit better than the Freundlich model. The maximum uptake of Cd(Ⅱ) by tourmaline was 31.77, 33.11 and 40.16 mg/g at pH 4.0 at 15, 25 and 35°C, respectively. Therefore, tourmaline is an effective adsorbent for the removal of Cd(Ⅱ) from acidic aqueous solutions. In addition, the kinetics for the Cd(Ⅱ) adsorption by tourmaline closely followed the pseudo-second-order model. The thermodynamic parameters indicated that adsorption was feasible, spontaneous, and endothermic. Furthermore, the pH variation after adsorption, ζ-potential, metal ions desorbed and released, and FT-IR analysis indicated that the physisorption and chemisorption mechanisms of tourmaline for heavy metals. These mechanisms included water that was automatically polarized by tourmaline, the ion exchange process, and electropolar adsorption. Among the mechanisms, the automatic polarization of water caused by tourmaline is a unique adsorption mechanism for tourmaline. | WANG CuiPing WANG BaoLin LIU JingTing YU Li SUN HongWen WU JiZhou | 2012 | Chinese Science Bulletin2012，57，24： | 11 |
| 2 | Numerical study of shoreline changes by emergency beach nourishment project at the Middle Beach of Beidaihe, China显示文摘Beach nourishment is a common coastal management strategy used to protect beach from erosion along the sandy coastlines. This method has been successfully applied to an emergency project at the West Beach of Beidaihe in the summer of 2008 and the full West Beach nourishment project in 2009-2010, which is the direct base of this study. Some basic information about the emergency engineering area at the Middle Beach is firstly described. The shoreline change of this area, including the analysis of beach width in five monitoring profiles in the bathing places of Middle Beach, is then discussed. After that a numerical model based on one-line theory is established and the numerical results agree well with the measured shorelines, which indicates that the model is appropriate and is qualified to predict the shoreline change of the Middle Beach. With the same model and parameters, long-term performance of the project is predicted, and the result shows that without follow-up nourishment and project, the bathing places can remain suitable for bathing for about 10 a. It is suggested to nourish the beach in time and carry out the beach nourishment project for the full Middle Beach in Beidaihe. | ZHANG Yu HE Lulu KUANG Cuiping PAN Yi GU Jie YANG Yanxiong ZHANG Jiabo | 2012 | Acta Oceanologica Sinica2012，31，1： | 4 |
| 3 | Marek's disease virus VP22 enhances potentially the immune response of E SAT-6/CFP-10 against Mycobacterium bovis infection显示文摘对 Mycobacterium bovis 的当前可得到的疫苗,肺结核的原因的代理人,别提供可靠功效,因此对有改进功效的一支新奇疫苗有需要。这里,我们使用蛋白质 transduction 技术交付直接表示 mycobacterial 抗原指向房间的 DNA 疫苗。我们使用了包括 VP22 的领域(PTD ) 蛋白质从 Marek 的疾病病毒 serotype 结合的各种各样的蛋白质 transduction 1 (MDV-1 ) ,作为为编码抗原的 DNA 构造的交货系统,早能分泌的 antigenic target-6 kDa (ESAT-6 ) 蛋白质和文化过滤蛋白质 10 (CFP-10 ) M。bovis。真核细胞的表示 plasmid pZ106,编码抗原 ESAT-6 和 CFP-10,结合了到各种各样的 PTD,被用来构造试验性的准备。我们的调查结果与 CFP-10 独自或在联合表明了那 VP22 : ESAT-6 熔化蛋白质能传播进包围 transfected 房间的房间单层的所有原子核。而激活 trans transcriptional PTD 出现了,熔化蛋白质和 8R 肽的有限交货是不能的交付熔化蛋白质进任何 untransfected 房间。我们证明了有熔化到 VP22 的准备的那免疫导致更高的抗体和干扰素 -- titer (P < 0.05 ) 。一起拿,我们的结果证明 MDV-1 VP22 用 DNA 疫苗在基因治疗和免疫用作潜在的有免疫力的 enhancer,为 M 的预防提供一条新奇途径。bovis 感染。 | Hongjun Chen Wei Wang Cuiping Song Shengqing Yu Chan Ding | 2010 | Acta Biochimica et Biophysica Sinica2010，42，5： | 4 |
| 4 | Effect of aging treatment on microstructure and corrosion behavior of Al-Zn-Mg aluminum alloy in aqueous solutions with different aggressive ions显示文摘The microstructure and corrosion behavior of Al-Zn-Mg alloy(namely 7×××)after natural aging treatment(NAT)and artificial aging treatment(AAT)in aqueous NaCl solutions containing different aggressive ions have been investigated in current work.Results of microstructure characterization demonstrate that the aging treatment has a great influence on the grain size and precipitates.The grain size is relatively sizeable and no evident precipitates are observed in alloy after NAT comparable with that after AAT.The electrochemical corrosion behavior of alloy was studied by polarization curve and electrochemical impedance spectroscopy(EIS).The corrosion potential(E_(corr))of the aluminum alloy is more negative in 3.5 wt.%NaCl containing 0.052 wt.%NaHSO_(3)solution than that in 3.5 wt.%NaCl solutions with or without 0.907 wt.%NaHCO_(3).Charge transfer resistance(Rct)results reveal that alloy after AAT has an enhancement of corrosion resistance compare with that after NAT.With the immersion time increasing,mostly pitting spreads over the surface of the alloy only in NaCl solution,whereas exfoliation corrosion mainly occurs in NaCl solutions containing NaHSO_(3)or NaHCO_(3). | Pan Liu Lulu Hu Qinhao Zhang Cuiping Yang Zuosi Yu Jianqing Zhang Jiming Hu Fahe Cao | 2021 | Journal of Materials Science & Technology2021，，5： | 3 |
| 5 | Optimized prime editing efficiently generates glyphosate-resistant rice plants carrying homozygous TAP-IVS mutation in EPSPS显示文摘Dear Editor,Prime editing is a novel and universal CRISPR-Cas-derived precise genome-editing technology and has great potentials for applications in basic plant research and crop molecular breeding(Anzalone et al.,2019).Although low efficiency has restrained the original prime editors(PEs)from being used as a routine tool for precise genome editing in plants,an iterative update of the PEs is removing this obstacle(Lin et al.,2021;Xu et al.,2022).Recently,the Liu group reported three optimization strategies for improving prime editing efficiency(Chen et al.,2021;Nelson et al.,2022).The first strategy is based on engineered prime editing guide RNAs(epegRNAs),which were generated by incorporating structured RNA motifs to the 3′terminus of pegRNAs.This strategy enhances pegRNA stability and prevents degradation of the 3′extension(Nelson et al.,2022).The second strategy is based on the optimized PE2 protein(PEmax),which harbors a SpCas9 variant with increased nuclease activity,an additional nuclear localization signal(NLS)sequences,and a new linker between nCas9 and reverse transcriptase(Chen et al.,2021).The third strategy is based on inhibition of DNA mismatch repair(MMR)in cells(Chen et al.,2021).In this work,we tested the optimized PEs generated with these three strategies in rice,demonstrating that the optimized PEs greatly improved prime editing efficiency in rice.We named the two optimized PEs ePE3max and ePE5max:the former is comprised of the PEmax protein,an epegRNA with evopreQ1 appended to its 3′end,and a nicking sgRNA;the latter is comprised of the ePE3max system and a dominant negative OsMLH1 variant for inhibiting MMR.Using the two optimized PEs,we efficiently generated homozygous and heterozygous T173I,A174V,and P177S(TAP-IVS)mutation in EPSPS in rice,which lays a solid foundation for rice non-transgenic glyphosate-resistance breeding. | Yuanyuan Jiang Yiping Chai Dexin Qiao Junya Wang Cuiping Xin Wei Sun Zhenghong Cao Yu Zhang Yun Zhou Xue-Chen Wang Qi-Jun Chen | 2022 | Molecular Plant2022，15，11： | 3 |
| 6 | The antioxidant activity of glucosamine hydrochloride in vitro显示文摘 | Ronge Xing Song Liu Zhanyong Guo Huahua Yu Cuiping Li Xia Ji Jinhua Feng Pengcheng Li | 2005 | Bioorganic & Medicinal Chemistry2005，，6： | 1 |
| 7 | Impact of allelopathic rice seedlings on rhizospheric microbial populations and their functional diversity显示文摘 | Lin Ruiyu Rong Hong Zhou Junjian Yu Cuiping Ye Chenying Chen Liangsheng Lin Wenxiong | 2007 | Acta Ecologica Sinica2007，，9： | 1 |
| 8 | Enhancing the Thermostability of a Novel β-agarase AgaB through Directed Evolution显示文摘 | Chao Shi Xinzhi Lu Cuiping Ma Yiming Ma Xiaoyan Fu Wengong Yu | 2008 | Applied Biochemistry and Biotechnology2008，，1： | 1 |
| 9 | The Mycoplasma gallisepticum α-enolase is cell surface-exposed and mediates adherence by binding to chicken plasminogen显示文摘 | Hongjun Chen Shengqing Yu Xinyue Shen Danqing Chen Xvsheng Qiu Cuiping Song Chan Ding | 2011 | Microbial Pathogenesis2011，，： | 1 |
| 10 | Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye 显示文摘 | Li Cuiping Yu Huahua Liu Song | 2005 | Bioorganic and Medicinal Chemistry Letters2005，15，： | 1 |
| 11 | Purification and Refolding of a Novel β-Agarase from Inclusion Body of E. coli显示文摘β-agarase AgaB appears to represent a new family of glycoside hydrolase; it is structurally and functionally different from other known agarases. In the present study, AgaB was expressed with a temperature-inducible expression system in E. coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag. The protein existed mainly in the form of inclusion body. After being washed and solubilized, AgaB in inclusion body was denatured and purified to electrophoretic purity by immobilized metal affinity chromatography. The purified AgaB was then refolded using a simple pulse dilution method, and the refolded AgaB showed a high specific hydrolysis activity of about 1600 units /mg protein. Forty milligrams of refolded pure protein were obtained from 1L of culture. | ZHANG Li, LU Xinzhi, HAN Feng, MA Cuiping, and YU Wengong* Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, P.R. China | 2007 | Journal of Ocean University of China2007，6，1： | 1 |
| 12 | Factors influencinghemolyticactivity of venom from the jellyfish Rhopilema esculentumKishinouye显示文摘 | YU Huahua LI Cuiping LI Ronggui | 2007 | Food and Chemical Toxicology2007，45，7： | 1 |
| 13 | MeRIP-PF:An Easy-to-use Pipeline for High-resolution Peak-finding in MeRIP-Seq Data显示文摘RNA modifications, especially methylation of the N6 position of adenosine (A)--m 6A, represent an emerging research frontier in RNA biology. With the rapid development of high-throughput sequencing technology, in-depth study of m 6A distribution and function relevance becomes feasible. However, a robust method to effectively identify m 6A-modified regions has not been available yet. Here, we present a novel high-efficiency and user-friendly analysis pipeline called MeRIP-PF for the signal identification of MeRIP-Seq data in reference to controls. MeRIP-PF provides a statistical P-value for each identified m 6A region based on the difference of read distribution when compared to the controls and also calculates false discovery rate (FDR) as a cut off to differentiate reliable m 6A regions from the background. Furthermore, MeRIP-PF also achieves gene annotation of m 6A signals or peaks and produce outputs in both XLS and graphical format, which are useful for further study. MeRIP-PF is implemented in Perl and is freely available at http://software.big.ac.cn/MeRIP-PF.html. | Yuli Li Shuhui Song Cuiping Li Jun Yu | 2013 | Genomics, Proteomics & Bioinformatics2013，11，1： | 1 |
| 14 | Recent progress on the hydrogen storage properties of ZrCo-based alloys applied in International Thermonuclear Experimental Reactor(ITER)显示文摘Under the development of International Thermonuclear Experimental Reactor(ITER) system aimed at realizing the controllable fusion reaction to solve the energy crisis fundamentally, there is an urgent need to find an appropriate material for tritium handling. ZrCo alloy is considered to be a promising candidate for the storage and delivery of hydrogen isotopes due to the favorable characteristics such as low plateau pressure for absorption, high dissociation pressure at moderate temperature and better ability of trapping ~3He. However, the hydrogen induced disproportionation and the slower recovery/deliverty rate of ZrCo-based alloys have limited their further application in ITER system. This review summarizes the efforts towards enhancing the hydrogen storage properties of ZrCo-based alloys including element substitution, surface modification, disproportionation mechanism investigation and the isotope effect study. Element substitution and surface modification play positive role to improve the anti-disproportionation ability and kinetic property of the alloys. However, the ZrCo-based alloys require to be further modified by more attempts such as new composition, novelty modification method or catalyst addition in order to better satisfy the application demands for tritium handling. Moreover, new insight for further understanding the inner disproportionation mechanisms of this material is needed by combining the advance characterization and theoretical analysis, which is in favor of addressing the disproportionation problem of the ZrCo-based alloys essentially. | Feng Wang Rongfeng Li Cuiping Ding Wukui Tang Yibo Wang Shimeng Xu Ronghai Yu Ying Wu | 2017 | Progress in Natural Science:Materials International2017，27，1： | 1 |
| 15 | Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye 显示文摘 | YU Huahua LI Cuiping LI Ronggui | 2007 | Food and Chemical Toxicology2007，45，7： | 1 |
| 16 | Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye显示文摘 | LI Cuiping YU Huahua LIU Song | 2005 | Bioorganic & Medicinal Chemistry Letters2005，24，15： | 1 |
| 17 | Community-based study of the association of subclinical thyroid dysfunction with blood pressure显示文摘 | Yu Duan Wen Peng Xiaodong Wang Wei Tang Xiaoyun Liu Shuhang Xu Xiaodong Mao Shangyong Feng Yu Feng Youwen Qin Kuanfeng Xu Cuiping Liu Chao Liu | 2009 | Endocrine2009，，2： | 1 |
| 18 | Performance evaluation of a beach nourishment project at west beach in beidaihe,China显示文摘 | KUANG Cuiping PAN Yi ZHANG Yu | 2011 | Journal of Coastal Research2011，27，4： | 1 |
| 19 | Approach of moving boundary and its application in 3D tidal current simulation of Changjiang (Yangtze) River Estuary based on ECOMSED model显示文摘Considering three-dimensional model ECOMSED can not simulate wetting-drying of shoal with its fixed boundary, an approach to represent moving boundary in the model is introduced here. This approach smoothly joints the internal and external mode by making use of wetting and drying tech-nique and is verified by a numerical test which presents a good agreement with the previous test results obtained by other researchers. A three dimensional numerical model is established to simu-late the hydrodynamics in spring tide in the Changjiang (Yangtze) River estuary by this modified ECOMSED model which is also validated through the observed field data, the simulation presents a good periodic tidal change. It also successfully simulates the tidal current of computational areas and reproduces the tidal flat intermittent appearance. | KUANG Cuiping SUN Bo LIU Shuguang GU Jie YU Weiwei | 2009 | Acta Oceanologica Sinica2009，28，4： | 1 |
| 20 | A two-dimensional morphological model based on next generation circulation solver II: Application to Caofeidian, Bohai Bay, China显示文摘 | KUANG Cuiping CHEN Siyu ZHANG Yu | 2012 | Coastal Engineering2012，59，1： | 1 |
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