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| 1 | Phosphorus recovery from biogas fermentation liquid by Ca-Mg loaded biochar显示文摘Shortage in phosphorus(P) resources and P wastewater pollution is considered as a serious problem worldwide. The application of modified biochar for P recovery from wastewater and reuse of recovered P as agricultural fertilizer is a preferred process. This work aims to develop a calcium and magnesium loaded biochar(Ca–Mg/biochar) application for P recovery from biogas fermentation liquid. The physico-chemical characterization, adsorption efficiency, adsorption selectivity, and postsorption availability of Ca-Mg/biochar were investigated. The synthesized Ca–Mg/biochar was rich in organic functional groups and in Ca O and Mg O nanoparticles. With the increase in synthesis temperature, the yield decreased, C content increased, H content decreased, N content remained the same basically, and BET surface area increased. The P adsorption of Ca–Mg/biochar could be accelerated by nano-Ca O and nano-Mg O particles and reached equilibrium after 360 min.The process was endothermic, spontaneous, and showed an increase in the disorder of the solid–liquid interface. Moreover, it could be fitted by the Freundlich model. The maximum P adsorption amounts were 294.22, 315.33, and 326.63 mg/g. The P adsorption selectivity of Ca–Mg/biochar could not be significantly influenced by the typical p H level of biogas fermentation liquid. The nano-Ca O and nano-Mg O particles of Ca–Mg/biochar could reduce the negative interaction effects of coexisting ions. The P releasing amounts of postsorption Ca–Mg/biochar were in the order of Ca–Mg/B600 > Ca–Mg/B450 > Ca–Mg/B300. Results revealed that postsorption Ca–Mg/biochar can continually release P and is more suitable for an acid environment. | Ci Fang Tao Zhang Ping Li Rongfeng Jiang Shubiao Wu Haiyu Nie Yingcai Wang | 2015 | Journal of Environmental Sciences2015，27，3： | 21 |
| 2 | Experimental studies on the Danxia landscape morphogenesis in Mt. Danxiashan, South China显示文摘Danxia 风景的形成太慢,不能在我们的生活时间被遵守, Danxia 风景在发展了的古气候与礼品显著地不同。因此,这研究采用了试验性的途径在丹克萨尚山,在各种各样的风景形态学的形成上检验 lithological 和 paleoclimatic 控制华南。122 件岩石样品的一个总数从象白点那样的 Danxia 风景形态学的一个范围被收集,通过洞,蜂房洞,水平沟,自然的桥,石头支柱,和鳞石头小、大。分析镇定样品在实验的不同类型被进行,包括测试的单轴的机械力量;对硫磺的酸侵蚀摇抵抗,结冰,并且融化;X 光检查荧光 spectrometry (XRF ) 分析;诱导地联合的血浆团 spectrometry (ICPMS ) 分析;并且在一台极化的显微镜下面的鉴定分析。结果显示各种各样的 Danxia 风景形态学的形成能源于至少一个下列过程:微分侵蚀由于 lithological 差别,化学溶解和再结晶,结冰并且融化的行动,酸腐蚀,河流动的捱过的、侧面的侵蚀,并且构造高举。在潮湿的季风气候和在第四级的冰川的阶段和间冰期的阶段的行动的集中的结冰并且融化的引申的水侵蚀能在 Danxia 风景地形学的形成上有大影响。 | ZHU Cheng WU Li ZHU Tongxin HOU Rongfeng HU Zhinong TAN Yan SUN Wei JIA Tianjiao PENG Hua | 2015 | Journal of Geographical Sciences2015，25，8： | 15 |
| 3 | Self-microemulsifying drug delivery system for improving the bioavailability of huperzine A by lymphatic uptake显示文摘Huperzine A(Hup-A) is a poorly water-soluble drug with low oral bioavailability. A selfmicroemulsifying drug delivery system(SMEDDS) was used to enhance the oral bioavailability and lymphatic uptake and transport of Hup-A. A single-pass intestinal perfusion(SPIP) technique and a chylomicron flow-blocking approach were used to study its intestinal absorption, mesenteric lymph node distribution and intestinal lymphatic uptake. The value of the area under the plasma concentration–time curve(AUC) of Hup-A SMEDDS was significantly higher than that of a Hup-A suspension(P <0.01).The absorption rate constant(K_a) and the apparent permeability coefficient(P_(app)) for Hup-A in different parts of the intestine suggested a passive transport mechanism, and the values of K_a and P_(app) of Hup-A SMEDDS in the ileum were much higher than those in other intestinal segments. The determination of Hup-A concentration in mesenteric lymph nodes can be used to explain the intestinal lymphatic absorption of Hup-A SMEDDS. For Hup-A SMEDDS, the values of AUC and maximum plasma concentration(C_(max)) of the blocking model were significantly lower than those of the control model(P<0.05). The proportion of lymphatic transport of Hup-A SMEDDS and Hup-A suspension were about 40% and 5%,respectively, suggesting that SMEDDS can significantly improve the intestinal lymphatic uptake and transport of Hup-A. | Fang Li Rongfeng Hu Bin Wang Yun Gui Gang Cheng Song Gao Lei Ye Jihui Tang | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 15 |
| 4 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 5 | Combining sum-difference and auxiliary beams for adaptive monopulse in jamming显示文摘Only in the presence of sidelobe jamming(SLJ),can the conventional adaptive monopulse technique null the jamming effectively and maintain the monopulse angle estimation accuracy simultaneously.While mainlobe jamming(MLJ) exists,the mainlobe of adaptive pattern will subject to serious distortion,which results in a failure of detecting and tracking targets by monopulse technique.Therefore,a monopulse angle estimation algorithm based on combining sum-difference beam and auxiliary beam is presented.This algorithm utilizes both high gain difference beams and high gain auxiliary beams for cancelling the mainlobe jammer and multiple sidelobe jammers(SLJs) while keeping an adaptive monopulse ratio.Theoretical analysis and simulation results indicate that the serious invalidation of monopulse technique in MLJ and SLJs scenarios is resolved well,which improves the monopulse angle accuracy greatly.Furthermore,the proposed algorithm is of simple implementation and low computational complexity. | Rongfeng Li Can Rao Lingyan Dai Yongliang Wang | 2013 | Journal of Systems Engineering and Electronics2013，24，3： | 13 |
| 6 | Role and effect of vein-transplanted human umbilical cord mesenchymal stem cells in the repair of diabetic foot ulcers in rats显示文摘Diabetic foot ulcer(DFU)is one of diabetic complications,which is frequently present and tormented in diabetes mellitus.Most multipotent mesenchymal stromal cells(MSCs)are capable of immune evasion,providing an allogeneic,ready-to-use,cell product option for therapeutic applications.The beneficial effect of MSCs for the treatment of a variety of traumatic injuries,such as open wounds,has been extensively explored.In this study,a rat DFU model was used to simulate the pathophysiology of clinical patients and to investigate the localization of human umbilical cord mesenchymal stem cells(hUC-MSCs)after intravenous transplantation and its role in DFU healing,so as to evaluate the potential of hUC-MSCs in the treatment of DFU.The diabetic rat model was established by streptozotocin injection,which was used to create full-thickness foot dorsal skin wounds to mimic DFU by a 6-mm skin biopsy punch and a Westcott scissor.The hUC-MSCs were transplanted through femoral vein,and the ulcer cicatrization situation and the fate of hUC-MSCs were evaluated.Our data suggest that intravenously transplantated hUC-MSCs have the ability to migrate and locate to the wound tissue and are helpful to wound healing in DFU rats,partly by regulating inflammation,trans-differentiation and providing growth factors that promote angiogenesis,cell proliferation and collagen deposition.Herein,we demonstrate that hUC-MSC transplantation is able to accelerate DFU healing in rats and transplantation of exogenous stem cells may be a potential strategy for clinical application in DFUs. | Rongfeng Shi Weishuai Lian Yinpeng Jin Chuanwu Cao Shilong Han Xiaohu Yang Suming Zhao Maoquan Li Hui Zhao | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 13 |
| 7 | Abscisic Acid Antagonizes Ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis显示文摘增加的证据表明了 abscisic 酸(骆驼毛的织物) 否定地调制乙烯生合成,尽管内在的机制仍然保持不清楚。为了识别因素,包含了,我们在 Arabidopsis 与改变的乙烯生产为骆驼毛的织物感觉迟钝的异种进行了一幅屏幕。ABI4 的主导的等位基因, abi4-152,在 ABI4 的 C 终点与 16-amino-acid 截断生产通常认为的蛋白质,减少乙烯生产。由对比, ABI4 的二后退的猛烈等位基因, abi4-102 和 abi4-103,导致增加的乙烯进化,显示 ABI4 否定地调整乙烯生产。进一步的分析证明乙烯生合成基因 ACS4, ACS8,和 ACO2 的 thatexpression 显著地在 abi4-152 被减少,但是在猛烈异种增加了,与骆驼毛的织物上的部分依赖。染色质 immunoprecipitation 量的 PCR 试金证明 ABI4 直接绑这些乙烯生合成基因的倡导者,那件骆驼毛的织物提高这个相互作用。包含截断的 ABI4-152 肽的熔化蛋白质在转基因的植物比它的全身的对应物积累了到高水平,建议 ABI4 被它的 C 终点使动摇。因此,我们的结果证明那件骆驼毛的织物否定地在 Arabidopsis 通过乙烯生合成基因 ACS4 和 ACS8 的调停 ABI4 的 transcriptional 压抑调整乙烯生产。 | Zhijun Dong Yanwen Yu Shenghui Li Juan Wang Saijun Tang Rongfeng Huang | 2016 | Molecular Plant2016，9，1： | 9 |
| 8 | The Low Energy X-ray telescope (LE) onboard the Insight-HXMT astronomy satellite显示文摘The Low Energy X-ray telescope(LE) is one of the three main instruments of the Insight-Hard X-ray Modulation Telescope(Insight-HXMT). It is equipped with Swept Charge Device(SCD) sensor arrays with a total geometrical area of 384 cm^2 and an energy band from 0.7 to 13 ke V. In order to evaluate the particle induced X-ray background and the cosmic X-ray background simultaneously, LE adopts collimators to define four types of Field Of Views(FOVs), i.e., 1.6°×6°, 4°×6°, 50°-60°×2°-6 oand the blocked ones which block the X-ray by an aluminum cover. LE is constituted of three detector boxes(LEDs) and an electric control box(LEB) and achieves a good energy resolution of 140 e V@5.9 ke V, an excellent time resolution of 0.98 ms, as well as an extremely low pileup(<1%@18000 cts/s). Detailed performance tests and calibration on the ground have been performed,including energy-channel relation, energy response, detection efficiency and time response. | Yong Chen WeiWei Cui Wei Li Juan Wang YuPeng Xu FangJun Lul YuSa Wang TianXiang Chen DaWei Han Wei Hu Yi Zhang Jia Huo YanJi Yang MaoShun Li Bo Lu ZiLiang Zhang TiPei Li ShuangNan Zhang ShaoLin Xiong Shu Zhang RongFeng Xue XiaoFan Zhao Yue Zhu YuXuan Zhu HongWei Liu YiJung Yang Fan Zhang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 8 |
| 9 | OSBPL2-disrupted pigs recapitulate dual features of human hearing loss and hypercholesterolaemia显示文摘Oxysterol binding protein like 2(OSBPL2), an important regulator in cellular lipid metabolism and transport, was identified as a novel deafness-causal gene in our previous work. To resemble the phenotypic features of OSBPL2 mutation in animal models and elucidate the potential genotypephenotype associations, the OSBPL2-disrupted Bama miniature(BM) pig model was constructed using CRISPR/Cas9-mediated gene editing, somatic cell nuclear transfer(SCNT) and embryo transplantation approaches, and then subjected to phenotypic characterization of auditory function and serum lipid profiles. The OSBPL2-disrupted pigs displayed progressive hearing loss(HL) with degeneration/apoptosis of cochlea hair cells(HCs) and morphological abnormalities in HC stereocilia, as well as hypercholesterolaemia. High-fat diet(HFD) feeding aggravated the development of HL and led to more severe hypercholesterolaemia. The dual phenotypes of progressive HL and hypercholesterolaemia resembled in OSBPL2-disrupted pigs confirmed the implication of OSBPL2 mutation in nonsydromic hearing loss(NSHL) and contributed to the potential linkage between auditory dysfunction and dyslipidaemia/hypercholesterolaemia. | Jun Yao Huasha Zeng Min Zhang Qinjun Wei Ying Wang Haiyuan Yang Yajie Lu Rongfeng Li Qiang Xiong Lining Zhang Zhibin Chen Guangqian Xing Xin Cao Yifan Dai | 2019 | Journal of Genetics and Genomics2019，46，8： | 7 |
| 10 | MiR-31 regulates the function of diabetic endothelial progenitor cells by targeting Satb2显示文摘Endothelial 与糖尿病在病人失灵被知道导致脉管的疾病,并且 endothelial 祖先房间(EPC ) 为脉管的内皮细胞层的功能的保藏是不可缺少的。MicroRNA-31 (miR-31 ) 被发现了能调制干细胞的区别。然而, miR-31 怎么在糖尿病的 EPC 工作,仍然是不清楚的。这研究的目的是调查 miR-31 怎么调整糖尿病的 EPC 功能。在当前的学习, miR-31 表示在正常、糖尿病的 EPC 之间被比较。Satb2 根据计算预言在 EPC 作为 miR-31 的一个机能上地相关的目标被认出。我们也以它的 anti-apoptotic 效果探索了 miR-31 的角色。在 miR-31 表示的显著举起在糖尿病的 EPC 被发现,并且这提高的表情在高葡萄糖下面导致了压制的房间增长。miR-31 指向 Satb2,这也被发现,导致 anti-apoptotic 效果和 EPC 的功能的维护。而且,展出的 Satb2 击倒在健康、糖尿病的题目的 EPC 的增长和移植上的禁止的效果,它显示出象 miR-31 overexpression 的一样的趋势。相反地, Satb2 的 overexpression 显示出相反的效果。而且, Satb2 的 overexpression 在健康、糖尿病的题目稀释了 miR-31-induced 迁居和形成殖民地的能力减小和 EPC 的 apoptosis 正式就职。在糖尿病的 EPC,提高了葡萄糖水平被发现到起来调整 miR-31 表示,它接着提高了 EPC 的 malfunction 和死亡。在结论,我们的结果显示 miR-31 的起来规定可以由指向 Satb2 在糖尿病位于 endothelial 机能障碍下面。 | Weishuai Lian Xiaoxiao Hu Rongfeng Shi Shilong Han Chuanwu Cao Kun Wang Maoquan Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，4： | 7 |
| 11 | Effect of Ti substitution on hydrogen storage properties of Zr_(1-x)Ti_xCo (x = 0, 0.1, 0.2, 0.3) alloys显示文摘Zr1-xTixCo(x = 0, 0.1, 0.2, 0.3) alloys were prepared by arc-melting method and the effect of Ti substitution on hydrogen storage properties was studied systematically. Hydrogen desorption pressure-composition-temperature(PCT) measurements were carried out using Sievert's type volumetric apparatus for ZrCo(at 473 K, 573 K and 673 K) and Zr1-xTixCo alloys(at 673 K), respectively. Products after dehydrogenation were characterized by X-ray diffraction(XRD). In addition, the kinetics of Zr1-xTixCo hydride was investigated at 473 K and 673 K,respectively, under hydrogen pressure of 5 MPa. Results showed that Ti substitution for Zr did not change the crystal structure of ZrCo phase.With the increase of temperature from 473 K to 673 K, the extent of disproportionation for ZrCo alloy increased. With Ti content increasing at 673 K, the desorption equilibrium pressure of Zr1-xTixCo-H2 systems elevated and the disproportionation reaction of Zr1-xTixCo alloys was inhibited effectively. Ti substitution decreased the kinetics rate and the effective hydrogen storage capacity of Zr1-xTixCo alloys slightly.Generally speaking, it was found that Zr0.8Ti0.2Co alloy had better anti-disproportionation property with less decrease of effective hydrogen storage capacity which was beneficial to tritium application in the International Thermonuclear Experimental Reactor(ITER). | Yamin Zhao Rongfeng Li Ruihe Tang Boyan Li Ronghai Yu Wei Liu Huaqin Kou Jianbo Meng | 2014 | Journal of Energy Chemistry2014，23，1： | 6 |
| 12 | Quantitative Analysis on Measure Results by Resistograh for Wood Decay of Ancient Architecture显示文摘The air-dried density, bending strength (MOR) and compressive strength parallel to grain of different grades of rotten wood were measured with test specimens of 100-130 year-old larch (Larix sp.) collected in Wu Ying Dian, the Forbidden City. In addition, the MOR of specimens was measured by Resistograph and the correlations were analyzed between resistance value and mechanical properties of wood. The correlations are significant for quantitative evaluation of wood structural condition by Resistograph. The results showed that wood air-dried density, strength properties and resistance values decreased significantly with the increase of decay degree, and there were remarkable differences among various grades of wood. Based on the rotten degree, the attenuation rate is in the following order: MOR> compressive strength parallel to grain > air-dried wood density > resistance value, and the above 4 indexes were 32%, 53%, 73% and 74% of those of intact wood, respectively, when the degree of rotten wood reached grade 3. Significant positive correlation at 0.01 level was showed between the wave trough values and wood air-dried density, MOR, compressive strength parallel to grain. The correlations between the wave peak values and the average value and air-dried wood density, MOR reached 0.01 level, and the correlations between the wave peak values and the average value and compressive strength parallel to grain reached 0.05 level. Based on the correlation between resistance value and physico-mechanical properties of wood, the values could be analyzed quantitatively. | WANG Xiaohuan HUANG Rongfeng ZHENG Hongkui LI Hua LIU Xiuying | 2006 | Chinese Forestry Science and Technology2006，5，4： | 6 |
| 13 | Adaptive detectors in the Krylov subspace显示文摘The validity of the application of the Krylov subspace techniques in adaptive filtering and detection is investigated. A new verification of the equivalence of two well-known methods in the Krylov subspace, namely the multistage Wiener filters(MWF) and the auxiliary-vector filtering(AVF), is given in this paper. The MWF and AVF are incorporated into two well-known detectors, namely, the adaptive matched filter(AMF) and Kelly's generalized likelihood ratio test(GLRT) including their diagonally loaded versions, which form new detectors.Compared to the conventional AMF, GLRT, and their diagonally loaded versions as well as the reduced-rank AMF and GLRT, the probabilities of detection(PDs) of the new detectors are improved especially when the sample support is low. More importantly, the new detectors are robust of the rank selection of the clutter subspace compared to the reduced-rank AMF and GLRT. These new detectors all possess asymptotic constant false alarm rate(CFAR) property. | LIU WeiJian XIE WenChong LI RongFeng WANG ZeTao WANG YongLiang | 2014 | Science China(Information Sciences)2014，57，10： | 5 |
| 14 | Hierarchically porous carbon foams for electric double layer capacitors显示文摘对便携式的电子设备的成长要求意味着力量采购的那个无足轻重的人逐渐地被寻求在以后。电的双层电容器(EDLC ) 正在答应候选人因为使用尽最大努力由于他们的高力量密度和突出的控告 / 解除骑车稳定性采购原料。三维(3D ) 自食其力基于碳的材料广泛地在小 EDLC 为使用被学习了。然而,为 3D 碳电极的主要挑战是在他们的内部空格的有限离子散开率。探讨这限制,为内部离子散开提供另外的隧道的层次地多孔的 3D 结构被建议了。此处,我们为 ultralight 的合成报导一个新化学方法(9.92 mg/cm 3) 3D 面对钾碳酸盐包含连接 glutaraldehydecross 的 chitosan aerogel 的碳化的多孔的碳泡沫(PCF ) 。电子显微镜学图象表明碳泡沫是包含一致地驱散的 macropores 的碳表的一个互连的网络。另外, Brunauer-Emmett-Teller 大小证实层次地多孔的结构。PCF 电极能完成的电气化学的数据表演当当前的密度从 0.5 ~ 100 A/g 被增加时,在 0.5 A/g 的当前的密度的 246.5 F/g 的一个突出的 gravimetric 电容,和 67.5% 的显著电容保留被观察。伪固态的对称的 supercapacitor 经由二的汇编被制作新 PCF 并且被发现交付一超离频在 2.8 Wh/kg 的精力密度的 25 kW/kg 的力量密度。这研究与高电容的性能表明 ultralight 和层次地多孔的碳泡沫的合成。 | Feng Zhan Tianyu Liu Guihua Hou Tianyi Kou Lu Yue Rongfeng Guan Yat Li | 2016 | Nano Research2016，9，10： | 5 |
| 15 | Argo floats revealing bimodality of large-scale mid-depth circulation in the North Atlantic显示文摘Analysis of Argo float trajectories at 1 000 m and temperature at 950 m in the North Atlantic between November 2003 and January 2005 demonstrates the existence of two different circulation modes with fast transition between them. Each mode has a pair of cyclonic-anticyclonic gyres. The difference is the location of the cyclonic gyre. The cyclonic gyre stretches from southeast to northwest in the first mode and from the southwest to the northeast in the second mode. The observed modes strongly affect the heat and salt transport in the North Atlantic. In particular, the second mode slows down the westward transport of the warm and saline water from the Mediterranean Sea. | CHU Peter C IVANOV Leonid M MELNICHENKO Oleg V LI Rongfeng | 2008 | Acta Oceanologica Sinica2008，27，2： | 4 |
| 16 | Nitrogen release characteristics of polyethylene-coated controlled-release fertilizers and their dependence on membrane pore structure显示文摘 | Xiangdong Yang Rongfeng Jiang Yangzheng Lin Yanting Li Juan Li Bingqiang Zhao | 2018 | Particuology2018，16，1： | 4 |
| 17 | Recent progress on biodegradable materials and transient electronics显示文摘Transient electronics(or biodegradable electronics)is an emerging technology whose key characteristic is an ability to dissolve,resorb,or physically disappear in physiological environments in a controlled manner.Potential applications include eco-friendly sensors,temporary biomedical implants,and datasecure hardware.Biodegradable electronics built with water-soluble,biocompatible active and passive materials can provide multifunctional operations for diagnostic and therapeutic purposes,such as monitoring intracranial pressure,identifying neural networks,assisting wound healing process,etc.This review summarizes the up-to-date materials strategies,manufacturing schemes,and device layouts for biodegradable electronics,and the outlook is discussed at the end.It is expected that the translation of these materials and technologies into clinical settings could potentially provide vital tools that are beneficial for human healthcare. | Rongfeng Li Liu Wang Deying Kong Lan Yin | 2018 | Bioactive Materials2018，3，3： | 4 |
| 18 | Hippocampal overexpression of TREM2 ameliorates high fat diet induced cognitive impairment and modulates phenotypic polarization of the microglia显示文摘Type 2 diabetes mellitus(T2DM)and Alzheimer's disease(AD)share several common pathophysiological features.Rare variants of triggering receptor expressed on myeloid cells 2(TREM2)increase the risk of developing AD,suggesting the involvement of TREM2 and innate immunity in AD development.It is still unknown whether TREM2 is related to cognitive impairment in T2DM.Here,we investigated the effects of the hippocampal overexpression of TREM2 on cognitive in long-term high-fat diet(HFD)-fed mice.Male C57BL/6J mice were maintained on HFD for 50 weeks.TREM2 was overexpressed in the hippocampus 36 weeks after HFD feeding using adeno-associated virus vector(AAV)-mediated gene delivery.The results showed that the HFD feeding induced rapid and persistent weight gain,glucose intolerance and significant impairments in learning and memory.Compared with AAV-con,AAV-TREM2 significantly ameliorated cognitive impairment without altering body weight and glucose homeostasis in HFD mice.The overexpression of TREM2 upregulated the synaptic proteins spinophilin,PSD95 and synaptophysin,suggesting the improvement in synaptic transmission.Dendritic complexity and spine density in the CA1 region were rescued after TREM2 overexpression.Furthermore,TREM2 markedly increased the number of iba-1/Arg-1-positive microglia in the hippocampus,suppressed neuroinflammation and microglial activation.In sum,hippocampal TREM2 plays an important role in improving HFD-induced cognitive dysfunction and promoting microglial polarization towards the M2 anti-inflammatory phenotype.Our study also suggests that TREM2 might be a novel target for the intervention of obesity/diabetes-associated cognitive decline. | Min Wu Maolin Liao Rongfeng Huang Chunxiu Chen Tian Tian Hongying Wang Jiayu Li Jibin Li Yuxiang Sun Chaodong Wu Qifu Li Xiaoqiu Xiao | 2022 | Genes & Diseases2022，9，2： | 4 |
| 19 | Dendrimers as carriers for contrast agents in magnetic resonance imaging显示文摘Magnetic resonance imaging (MRI) is a non-invasive clinical imaging modality,which has become widely used in the diagnosis of human diseases around the world.Some MRI exams include the use of contrast agents.The goal of an ideal MRI contrast agent involves the tissue-or organ-targeting materials with high relaxivity and specificity,low toxicity and side effects,suitable long intravascular duration and excretion time and high contrast enhancement with low doses,in vivo,all coupled to low overall cost.Dendrimers are synthetic,highly branched,mono-disperse macromolecules of nanometer dimensions.Properties associated with these dendrimers such as uniform size,water solubility,modifiable surface functionality and available internal cavities make them candidates for ideal carriers of MRI contrast agents.The research progress of the dendritic contrast agents is discussed. | YAN GuoPing AI ChaoWu LI Liang ZONG RongFeng LIU Fan | 2010 | Chinese Science Bulletin2010，55，27： | 3 |
| 20 | Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs显示文摘Clinical xenotransplantations have been hampered by human preformed antibody-mediated damage of the xenografts.To overcome biological incompatibility between pigs and humans,one strategy is to remove the major antigens[Gal,Neu5 Gc,and Sd(a)]present on pig cells and tissues.Triple gene(GGTAI,CMAH,and β4 GalNT2)knockout(TKO)pigs were produced in our laboratory by CRISPR-Cas9 targeting.To investigate the antigenicity reduction in the TKO pigs,the expression levels of these three xenoantigens in the cornea,heart,liver,spleen,lung,kidney,and pancreas tissues were examined.The level of human IgG/IgM binding to those tissues was also investigated,with wildtype pig tissues as control.The results showed that aGal,Neu5 Gc,and Sd(a)were markedly positive in all the examined tissues in wildtype pigs but barely detected in TKO pigs.Compared to wildtype pigs,the liver,spleen,and pancreas of TKO pigs showed comparable levels of human IgG and IgM binding,whereas corneas,heart,lung,and kidney of TKO pigs exhibited significantly reduced human IgG and IgM binding.These results indicate that the antigenicity of TKO pig is significantly reduced and the remaining xenoantigens on porcine tissues can be eliminated via a gene targeting approach. | Ronggen Wang Miaomiao Ruan Runjie Zhang Lei Chen Xiaoxue Li Bin Fang Chu Li Xueyang Ren Jiying Liu Qiang Xiong Lining Zhang Yong Jin Lin Li Rongfeng Li Ying Wang Haiyuan Yang Yifan Dai | 2019 | The Journal of Biomedical Research2019，33，4： | 3 |
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