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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Development of "Purple Endosperm Rice" by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System显示文摘Anthocyanins 在主食庄稼的 anthocyanin 生合成的高抗氧化剂活动,和工程例如米饭(Oryza sativa L.) ,能为改进人的健康提供支持健康的食物。然而,为 biofortification 设计新陈代谢的小径仍然保持困难,并且因为它的 biosynthetic 小径的复杂基因规章的网络,在米饭内乳设计 anthocyanin 生产的以前的尝试失败了。在这研究,我们为叠的 transgene 开发了一个高效率的向量系统并且使用了它在米饭内乳设计 anthocyanin 生合成。我们做了包含内乳特定的倡导者驾驶的八 anthocyanin 相关的基因(从从薄荷科植物的玉米和六结构的基因 ?的二规章的基因) 的构造,加为标记切除的一个可选的标记和基因。有这构造的米饭的转变产生了新奇 biofortified germplasm 紫色内乳瑞斯(用汉语的叫的 Zijingmi ) ,它在内乳有高 anthocyanin 内容和抗氧化剂活动。这 anthocyanin 生产源于 transgenes 和产生激活的表示(或改进) 13 内长的 anthocyanin 生合成基因的表示,那是 silenced 或在野类型的米饭内乳在底层表示。这研究为叠的 transgene 提供一个有效、万用的工具箱并且为一条复杂生物小径的成功的工程表明它的使用,建议为在工厂的农学的特点的合成生物学和改进的这个工具箱的潜在的用途。 | Qinlong Zhu Suize Yu Dongchang Zeng Hongmei Liu Huicong Wang Zhongtang Yang Xianrong Xie Rongxin Shen Jiantao Tan Heying Li Xiucai Zhao Qunyu Zhang Yuanling Chen Jingxing Guo Letian Chen Yao-Guang Liu | 2017 | Molecular Plant2017，10，7： | 47 |
| 3 | DRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications显示文摘 | Xingliang Ma Qinlong Zhu Yuanling Ghen Yao-Guang Liu | 2016 | Molecular Plant2016，9，7： | 39 |
| 4 | Responses of Nitrogen and Related Enzyme Activities to Fertilization in Rhizosphere of Wheat显示文摘In the present experiment, wheat seedlings (Trticucum aestivum L.) were grown on a purple soil withvarious fertilizer treatments in order to investigate the responses of nitrogen and related eusyme activitiesin the rhizosphere. The results revealed the increments of both organic matter and total N in the soil withthe proximity to the growing roots, especially in the treatment of supplying pig manure in combination withchemical fertilizer, suggesting that they could come from root and microorganism exudation which could beintensified by inorganic-organic fertilization, being of benefit to improving the physical and biological envi-ronment in the rhizosphere of wheat. Much more inorganic N was observed in the fertilized soils surroundingwheat roots than in the CK treatment, indicating the improvement of crop N supply in the rhisosphere ofwheat by fertilization.The activities of invertase, urease and protease in the root zone were greatly enhanced as compared tothose in the other parts of soils except that the urease activities were similar in the rhizo8phere and nonrhi-zosphere of the CK and pig manure treatments, indicating that invertase and protease could be produced bygrowing roots and rhizosphere microorganisms, in contrast to urease which could be stimulated by urea. Also,significant increment of these three enzyme activities in the rhizosphere of wheat by fertilizatioll, especially byapplication of chemical fertilizer combined with pig manurel suggested that fertilization not only acceleratedthe renewal of organic matter but also enhanced bioavailability of organic N in that soil zone. This couldbe the reason why the total amount of inorganic N available for plants was increased more obviously in therhizosphere of wheat of the fertilization treatments than in that of the CK treatment. | YUANLING YUSHANGQI 等 | 1997 | Pedosphere1997，7，2： | 29 |
| 5 | Changes in phototactic behavior of Daphnia magna clone C_1242 in response to copper, cadmium and pentachlorophenol显示文摘In order to develop a sound biotechnique for monitoring water quality that builds on the previous experiments carried out in our laboratory, a specific D. magna clone C 1242 was used to study the effects of pollutants on phototactic behavior. In all experiments, the animals showed a stable and repeatable phototactic index approximated 0.2 in the presence and 0.4 in the absence of fish kairomones, which decreased significantly in response to pollutants. There existed no pollutant×fish kairomone interaction, indicating the changes in phototactic behavior of animals imposed by pollutants were independent of the presence of fish kairomones. The detection limits for changes in phototactic behavior of D. magna clone C 1242 are 0.04 mg/L for copper, 0.02 mg/L for cadmium, and 0.80 mg/L for PCP, respectively, quite lower than LC 50(48 h). The changes in phototactic behavior in presence to pollutants occurred quickly(3 h) compared to the period over whole acute toxicity tests. Therefore, D. magna clone C 1242 could be potentially used to monitor water quality. Moreover, the phototactic behavior did not decrease further in the pollutant mixtures employed in our experiments compared to individual pollutants, except in the Cd-PCP treatment. This fact suggests that the formation of water quality criteria must be based upon pollutant mixture tests. | YUANLing E.Michels L.DeMeester | 2003 | Journal of Environmental Sciences2003，15，6： | 20 |
| 6 | A Novel Rice bHLH Transcription Factor, DTD, Acts Coordinately with TDR in Controlling Tapetum Function and Pollen Development显示文摘Dear Editor, Male reproductive development is an essential biological process for flowering plants and crucial for crop seed production.Formation of the male reproductive organ,the anther,involves a number of developmental events,including stamen meristem specification,generation of sporogenous cells and their differentiation into microspore mother cells (MMCs),meiosis,microspore (pollen) maturation,and pollination (Ma,2005).The formation of microspores and their development into mature pollen grains require cooperative interactions between gametophytic (microspores) and sporophytic (anther wall) cells,with the innermost cell layer,the tapetum,playing the most crucial role (Ma,2005).Tapetal cells undergo degeneration by programmed cell death (PCD). | Chonghui Ji Heying Li Libin Chen Min Xie Fengping Wang Yuanling Chen Yao-Guang Liu | 2013 | Molecular Plant2013，6，5： | 14 |
| 7 | A study of SAR remote sensing of internal solitary waves in the north of the South China Sea:Ⅰ.Simulation of internal tide transformation显示文摘For settlement of the well-known problem of contemporary radar imaging models,i.e.,the problem of a general underestimation of radar signatures of hydrodynamic features over oceanic internal waves and underwater bottom topography in tidal waters at high radar frequency bands(X-band and C-band),the impact of the ocean surface mixed layer turbulence and the significance of stratified oceanic model on SAR remote sensing of internal solitary waves are proposed.In the north of the South China Sea by utilizing some observed data of background field the nonlinearity coefficient,the dispersion coefficient,the horizontal variability coefficient and the phase speed in the generalized K-dV equation are determined approximately.Through simulations of internal tide transformation the temporal evolution and spatial distribution of the vertical displacement and horizontal velocity of internal wave field are obtained.The simulation results indicate that the maximum amplitudes of internal solitary waves occur at depth 35 m,but the maximum current speeds take place at depth 20 m in this area of the sea(about 20°30'N,114°E)in August.It was noticed that considering the effects of flood current and ebb current respectively is appropriate to investigate influence of the background shear flow on coefficients of the K-dV equation.The obtained results provide the possibility for the simulation of SAR signatures of internal solitary waves under considering the impact of ocean surface mixed layer turbulence in the companion paper. | FAN Zhisong ZHANG Yuanling SONG Mei | 2008 | Acta Oceanologica Sinica2008，27，4： | 12 |
| 8 | Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes显示文摘新基因起源是授与 phenotypic 变化和生物差异的 genomic 革新的主要来源。在植物的新 mitochondrial 基因的产生可以引起细胞质的男绝育(厘米) ,它能支持 outcrossing 和增加健康。然而, mitochondrial 基因怎么在结构和功能发源并且演变,仍然保持不清楚。米饭厘米的野未成功的类型被 mitochondrial 基因 WA352c (以前命名的 WA352 ) 授与并且广泛地在混合米饭繁殖被利用了。这里,我们重建由 11 mitochondrial genomic recombinant 的鉴定和分析的 WA352c 的进化轨道在野、栽培的米饭与 WA352c 有关组织。我们推断这些结构在野米饭 Oryza rufipogon,结合的 substoichiometric 变和顺序变化的 mitochondrial 染色体在保存 mitochondrial 序列之中通过多重重新整理产生了。我们鉴别二表示了,但是在这些结构之中的 nonfunctional protogenes,和他们能发展进功能的厘米基因经由的表演定序能减轻蛋白质的自我禁止的潜力的变化。这些顺序变化将资助蛋白质与编码原子核的 mitochondrial 蛋白质 COX11 交往的能力,在花药 tapetum 和男绝育导致早熟的规划房间死亡。而且,我们证明把 COX11 相互作用领域编码为这些 WA352c 相关的基因的序列经历了在进化期间净化选择。我们经由在结构,顺序,拷贝数字,和功能包含渐渐的变化的 multi-recombination/protogene formation/functionalization 机制为新厘米基因的形成和进化建议一个模型。 | Huiwu Tang Xingmei Zheng Chuliang Li Xianrong Xie Yuanling Chen Letian Chen Xiucai Zhao Huiqi Zheng Jiajian Zhou Shan Ye Jingxin Guo Yao-Guang Liu | 2017 | Cell Research2017，27，1： | 11 |
| 9 | A study of SAR remote sensing of internal solitary waves in the north of the South China Sea:Ⅱ.Simulation of SAR signatures of internal solitary waves显示文摘A new model developed from the full-spectrum model of Lyzenga and Bennett (1988) is built up by us preliminarily through considering the impact of the ocean surface mixed layer turbulence on SAR remote sensing of internal solitary waves. In the partial differential equation of the action spectral density of the surface gravity-capillary waves the source function representing the contribution of the turbulence is added besides the usual source function representing the contribution of the wind. The source function is determined by applying the k-ε model and adopting the Nasmyth spectrum of oceanic turbulence (Nasmyth,1970; Oakey,1982; Fan,2002) on the basis of the previous simulation results of internal tide transformation obtained in the companion paper (Fan et al.,2008). Either under relatively high wind speed,or under low wind speed,our model predicts significant large modulations of radar backscatter at all three bands (L,C and X bands) for both VV and HH polarization. These results prove that considering the impact of ocean surface mixed layer turbulence on SAR remote sensing of internal solitary waves is reasonable and appropriate for settlement of the well-known problem of contemporary radar imaging models. | FAN Zhisong ZHANG Yuanling SONG Mei | 2008 | Acta Oceanologica Sinica2008，27，5： | 10 |
| 10 | Genome-editing technologies: the gap between application and policy显示文摘Genome editing is a new technology for manipulating genomic DNA sequences at specific sites,and researchers worldwide are rapidly developing new variants of genomeediting applications that target mRNAs,viruses,chromatin structure,and other cellular processes. | Gousi Li Yao-Guang Liu Yuanling Chen | 2019 | Science China(Life Sciences)2019，62，11： | 9 |
| 11 | The long-term prediction of the oil-contaminated water from the Sanchi collision in the East China Sea显示文摘The condensate and bunker oil leaked from the Sanchi collision would cause a persistent impact on marine ecosystems in the surrounding areas. The long-term prediction for the distribution of the oil-polluted water and the information for the most affected regions would provide valuable information for the oceanic environment protection and pollution assessment. Based on the operational forecast system developed by the First Institute of Oceanography, State Oceanic Administration, we precisely predicted the drifting path of the oil tanker Sanchi after its collision. Trajectories of virtual oil particles show that the oil leaked from the Sanchi after it sank is mainly transported to the northeastern part of the sink location, and quickly goes to the open ocean along with the Kuroshio. Risk probability analysis based on the outcomes from the operational forecast system for years 2009 to2017 shows that the most affected area is at the northeast of the sink location. | YIN Liping ZHANG Min ZHANG Yuanling QIAO Fangli | 2018 | Acta Oceanologica Sinica2018，37，3： | 9 |
| 12 | Adsorption characteristics of a novel ceramsite for heavy metal removal from stormwater runoff显示文摘Urban sediments have rapidly increased in recent years around the world,and their effective management has become an important problem.To remove heavy metals from stormwater runoff and use sediments as a resource,a novel ceramsite was developed using sewer pipe sediments(SPS),river bed sediments(RBS),urban water supply treatment sludge(WSTS),and wastewater treatment plant excess sludge(WWTS).The optimal composition was determined based on the Brunauer–Emmett–Teller specific surface area and an orthogonal test design.The adsorption characteristics of the novel ceramsite for dissolved heavy metals(Cu^(2+)and Cd^(2+)) were investigated through adsorption isotherms and kinetic experiments at(25±1)℃.Both Cu^(2+) and Cd^(2+) were effectively removed by the novel ceramsite,and their equilibrium adsorption was 4.96 mg·g^(-1) and 3.84 mg·g^(-1),respectively.Langmuir isotherms and a pseudo-first-order kinetic equation described the adsorption process better than other techniques.Characterization analysis of the ceramsite composition before and after heavy metal adsorption showed that the Cu^(2+) and Cd^(2+) contents in the ceramsite increased after adsorption.The results revealed that adsorption is both a physical and chemical process,and that ceramsite can be used as a bioretention medium to remove heavy metals from stormwater runoff while simultaneously converting problematic urban sediments into a resource. | Jianlong wang Yuanling Zhao Pingping Zhang Liqiong Yang Huai'ao Xu Guangpeng Xi | 2018 | Chinese Journal of Chemical Engineering2018，26，1： | 9 |
| 13 | ALMOST SURE AND MOMENT EXPONENTIAL STABILITY OF PREDICTOR-CORRECTOR METHODS FOR STOCHASTIC DIFFERENTIAL EQUATIONS显示文摘这份报纸几乎肯定处理并且预言者修正者方法的一个班的指数的稳定性用于它的随机的微分方程的时刻 ? 类型。为这类方法的稳定性标准被导出。方法被显示几乎肯定维持并且时刻为在适当条件下面的所有足够地小的 timesteps 的指数的稳定性。一个数字实验进一步证明这些理论结果。 | Yuanling NIU Chengjian ZHANG | 2012 | Journal of Systems Science & Complexity2012，25，4： | 4 |
| 14 | Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants显示文摘Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including plants.The system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site. | Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen Jianian Tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong Xie Qinlong Zhu Yuanling Chen Xiucai Zhao Jingxin Guo Yao-Guang Liu | 2020 | Science China(Life Sciences)2020，63，6： | 4 |
| 15 | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica显示文摘Recent studies indicated that type II Toxoplasma gondii(Tg)GRA15II favored the generation of classically activated macrophages(M1),whereas type I/III TgROP16I/III promoted the polarization of alternatively activated macrophages(M2).A number of studies have demonstrated that M2 cells are involved in the pathogenesis of the liver fibrogenesis caused by Schistosoma japonicum.The purpose of the present study was to explore the inhibitory effect of Toxoplasma-derived TgGRA15II on mouse hepatic fibrosis with schistosomiasis.The gra15II and rop16I/III genes were amplified from strains T.gondii PRU and Chinese 1 Wh3,respectively.Lentiviral vectors containing the gra15II or rop16I/III plasmid were constructed and used to infect the RAW264.7 cell line.The polarization of the transfected cells was evaluated,followed by co-culture of the biased macrophages with mouse hepatic stellate JS1 cells.Then,mice were injected with GRA15II-driven macrophages via the tail vein and infected with S.japonicum cercariae.TgGRA15II induced a M1-biased response,whereas TgROP16I/III drove the macrophages to a M2-like phenotype.The in vitro experiments indicated that JS1 cell proliferation and collagen synthesis were decreased following co-culture with TgGRA15II-activated macrophages.Furthermore,mice inoculated with TgGRA15II-biased macrophages displayed a notable alleviation of collagen deposition and granuloma formation in their liver tissues.Our results suggest that TgGRA15II-induced M1 cells may dampen the M2 dominant pathogenesis of hepatic fibrosis and granulomatosis.These results provide insights into the use of parasite-derived immunomodulators as potential anti-fibrosis agents and to re-balance the schistosomiasis-induced immune response. | Yuanyuan Xie Huiqin Wen Ke Yan Shushu Wang Xuesong Wang Jian Chen Yuanling Li Yuanhong Xu Zhengrong Zhong Jilong Shen Deyong Chu | 2018 | Cellular & Molecular Immunology2018，15，2： | 4 |
| 16 | Improved two-way satellite time and frequency transfer with Multi-GEO in BeiDou navigation system显示文摘Due to the limitation of Geostationary Earth Orbit(GEO)satellite resources,only very few TWSTFT links with parallel counterpart operating via diferent GEO satellites between two ground stations are available.There are 5 GEO satellites in BeiDou Navigation System,which enables an improved TWSTFT with Multi-GEO.Two algorithms are put forward to take the advantage of Multi-GEO links to improve the performance of time and frequency transfer respectively.The dynamic weight average algorithm based on a newly proposed dynamic time variance aiming at improving the performance of time transfer is shown to be able to reduce the dynamic time deviation of the two-way measurement result to 1/√N dynamic time deviation of the single link using N-GEO links,if each link has similar level of link noises.The algorithm of cancelling out the link noise Allan variances with 2-GEO links is expected to obtain the Allan variance of clock diference between two ground stations.The experiment results using 2-GEO links in BeiDou Navigation System show that the dynamic weight average algorithm can improve the dynamic time deviation of the two-way measurement to around 0.6 of that of the single link in an average concept.And the estimated Allan deviation of the clock diference using the algorithm for cancelling the noise Allan variances of both links can reach 7×10 12(τ0.65)for the averaging time 1–105s which is within the same order of magnitude as the estimated Allan deviation based on carrier Doppler measurement for the averaging time 10 s–104s,despite the noisy links with the Allan deviation follows 3×10 10(τ1). | YANG WenKe GONG Hang LIU ZengJun LI YuanLing SUN GuangFu | 2014 | Science China(Information Sciences)2014，57，2： | 4 |
| 17 | STRONG PREDICTOR-CORRECTOR APPROXIMATION FOR STOCHASTIC DELAY DIFFERENTIAL EQUATIONS显示文摘 | Yuanling Niu Chengjian Zhang Kevin Burrage | 2015 | Journal of Computational Mathematics2015，33，6： | 3 |
| 18 | The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice显示文摘 | Yongyao Xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu Yuanling Chen Yoji Kawano Yao-Guang Liu Letian Chen | 2018 | Molecular Plant2018，11，5： | 3 |
| 19 | Nitrogen Status and Utilization in Rhizosphere of Rice显示文摘NitrogenStatusandUtilizationinRhizosphereofRiceHUANGJIAN-GUOandYUANLING(INRA,Microbiologie,CentredeNancy,54280Champenoux)(Fra... | HUANGJIAN-GUO YUANLING | 1995 | Pedosphere1995，5，4： | 2 |
| 20 | Geochemistry, Petrology and Mineralogy of Coal Measure Shales in the Middle Jurassic Yanan Formation from Northeastern Ordos Basin, China: Implications for Shale Gas Accumulation显示文摘The Jurassic Yanan Formation is one of the most important coal-producing formations and hydrocarbon source rocks in the Ordos Basin, North China. To evaluate the shale gas potential of the Yanan shale, a total of 48 samples from north Ordos Basin were sampled, and their geochemical, petrological, mineralogical and pore characteristics were investigated. It was found that the shale samples are a suite of early mature source rock. The total organic carbon(TOC) content ranges from 0.33% to 24.12% and the hydrogen index(HI) ranges from 43.31 mg/g to 330.58 mg/g. The relationship between Tmax and HI indicates the organic matter is type Ⅱ-Ⅲ. This conclusion is also supported by the organic petrological examination results, which shows that the kerogen is mainly liptinite and vitrinite. Minerals in the samples are composed mainly of quartz, clay and feldspar, and the clay minerals are composed of prevailing kaolinite, illite/smectite, chlorite and a small amount of illite. Under scanning electron microscope, OM pores in the Yanan shale are scarce except pores come from the kerogen intrinsic texture or clay aggregates within the organic particles. As the weak compaction caused by shallow burial depth, interparticle pores and intraparticle pores are common, the hydrocarbon storage capacity of the Yanan shale was improved. According to evaluation, the Yanan shale is considered as a good shale gas reservoir, but its hydrocarbon potential is more dependent on biogenic and coal-derived gas as the thermogenic gas is limited by the lower thermal maturity. | CHEN Qian YAN Xiangbin GUO Yuanling HONG Taiyuan NIE Haikuan ZHANG Jinchuan TANG Xuan LI Wanjun LIU Chong | 2018 | Acta Geologica Sinica(English Edition)2018，92，6： | 2 |
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