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| 1 | Mucosal cytokine network in inflammatory bowel disease显示文摘Inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn's disease (CD) are characterized by ongoing mucosal inflammation in which dysfunction of the host immunologic response against dietary factors and commensal bacteria is involved. The chronic in-flammatory process leads to disruption of the epithelial barrier, and the formation of epithelial ulceration. This permits easy access for the luminal microbiota and dietary antigens to cells resident in the lamina pro-pria, and stimulates further pathological immune cell responses. Cytokines are essential mediators of the interactions between activated immune cells and non-immune cells, including epithelial and mesenchymal cells. The clinical efficacy of targeting TNF-α clearly indicates that cytokines are the therapeutic targets in IBD patients. In this manuscript, we focus on the bio-logical activities of recently-reported cytokines [Inter-leukin (IL)-17 cytokine family, IL-31 and IL-32], which might play a role through interaction with TNF-α in the pathophysiology of IBD. | Akira Andoh Yuhki Yagi Makoto Shioya Atsushi Nishida Tomoyuki Tsujikawa Yoshihide Fujiyama | 2008 | World Journal of Gastroenterology2008，14，33： | 21 |
| 2 | MK615 decreases RAGE expression and inhibits TAGE-induced proliferation in hepatocellular carcinoma cells显示文摘AIM:To investigate the proliferative effect of advanced glycation end-products(AGEs) and the role of their cellular receptor(RAGE) on hepatocellular carcinoma(HCC) cells,and the inhibitory effects of MK615,an extract from Japanese apricot,against AGEs were also evaluated.METHODS:Two HCC cell lines,HuH7 and HepG2,were used.Expression of RAGE was investigated by poly-merase chain reaction,Western blotting,and flow cytemetry(FACS).The effect of MK615 on RAGE expression was also evaluated by FACS.The proliferative effects of a control(unglycated bovine serum albumin),glucosederived AGEs(Glc-AGE),and glyceraldehyde-derived AGEs(Glycer-AGE),and the anti-proliferative effect of MK615 against AGEs,were evaluated using MTT assays.RESULTS:Expression of RAGE was confirmed at both the mRNA and protein levels in both HuH7 and HepG2.FACS revealed that the level of RAGE expression was higher in HuH7 than in HepG2.Treatment with 0.1 μg/mL MK615 decreased the expression level of RAGE from 24.3% to 3.7% in HuH7 and from 6.2% to 4.8% in HepG2.The growth indices for the control,Glc-AGE,and Glycer-AGE were 1.06 ± 0.08,0.99 ± 0.04,and 1.38 ± 0.05,respectively,in HuH7(P = 0.037),and were 1.03 ± 0.04,1.04 ± 0.03,and 1.07 ± 0.05,respectively,in HepG2(P > 0.05).When the cells were cultured simultaneously with Glycer-AGE and MK615,MK615 abrogated the proliferative effect of Glycer-AGE in HuH7.CONCLUSION:Only Glycer-AGE has a proliferative effect on HuH7,which expresses a higher level of RAGE.MK615 suppresses the proliferative effect of GlycerAGE on HuH7 by decreasing the expression of RAGE. | Yuhki Sakuraoka Tokihiko Sawada Toshie Okada Takayuki Shiraki Yoshikazu Miura Katsuya Hiraishi Tatsushi Ohsawa Masakazu Adachi Jun-ichi Takino Masayoshi Takeuchi Keiichi Kubota | 2010 | World Journal of Gastroenterology2010，16，42： | 6 |
| 3 | Interleukin-33 expression is specifically enhanced in inflamed mucosa of ulcerative colitis显示文摘 | Ayako Kobori Yuhki Yagi Hirotsugu Imaeda Hiromitsu Ban Shigeki Bamba Tomoyuki Tsujikawa Yasuharu Saito Yoshihide Fujiyama Akira Andoh | 2010 | Journal of Gastroenterology2010，，10： | 2 |
| 4 | Roles of prostaglandin I2 and thromboxane A2 in cardiac ischemia-reperfusion iniury: a study using mice lacking their respectivereceptors显示文摘 | Xiao CY Hara A Yuhki K el al | 2001 | Circulation2001，104，18： | 1 |
| 5 | Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat 显示文摘 | Lopez J V Yuhki N Masuda R | 1994 | J Mol Evol1994，39，： | 1 |
| 6 | Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections显示文摘AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. RESULTS: Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. CONCLUSION: We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC. | Yuhki Sakuraoka Tokihiko Sawada Takayuki Shiraki Kyunghwa Park Yuhichiro Sakurai Naohisa Tomosugi Keiichi Kubota | 2012 | World Journal of Gastroenterology2012，18，28： | 1 |
| 7 | The relationship between delayed traumatic intracrebral hematoma and coagulopathy in patients diagnosed with traumartic subarachnoid hemorrhage显示文摘 | Sawauchi S Yuhki K Abe T | 2001 | No Shinkei Ceka2001，29，： | 1 |
| 8 | The relationship between delayed traumatic intracerebral hematoma and coagulopathy in patients diagnosed with a traumatic subarachnoid hemorrhage显示文摘 | Sawauchi S Yuhki K Abe T | 2001 | No Shinkei Geka2001，29，2： | 1 |
| 9 | Nature and origin of polymorphism in feline MHC class Ⅱ DRA and DRB genes显示文摘 | Yuhki N O'Brien SJ | 1997 | J Immunol1997，158，6： | 1 |
| 10 | Prostaglandin recep- tors EP2, EP3, and IP mediate exudate formation in car- rageenin-induced mouse pleurisy显示文摘 | Yuhki K Ueno A Naraba H | 2004 | J Pharmacol Exp Ther2004，311，3： | 1 |
| 11 | Molecular genetics and evolution of melanism in the cat family 显示文摘 | EIZIRIK E YUHKI N JOHNSON W E | 2003 | Current Biology2003，13，5： | 1 |
| 12 | The relationship between delayed traumatic intracerebral hematoma and coagulopathy in patients diagnosed with a traumatic subarachnoid hemorrhage显示文摘 | Sawauehi S Yuhki K Abe T | 2001 | No Shinkei Geka2001，29，2： | 1 |
| 13 | Doxorubicin induces apoptosis by activation of caspase-3 in cultured cardiomyocytes in vitro and rat cardiac ventricles in vivo 显示文摘 | Ueno M Kakinuma Y Yuhki K | 2006 | J PharmacolSci2006，101，2： | 1 |
| 14 | Roles of prostaglandin I(2) and thromboxane A(2) in cardiac ischemia-reperfusion injury:a study using mice lacking their respective receptors显示文摘 | Xiao CY Hara A Yuhki K | 2001 | Circulation2001，104，18： | 1 |
| 15 | Mo- lecular genetics and evolution of melanism in the cat family显示文摘 | EIZIRIK E YUHKI N JOHNSON W E | 2003 | Curt Biol2003，13，5： | 1 |
| 16 | The relationship between delayed traumatic intracerebral hematoma and coagulopathy in patients diagnosed with a traumatic subarachnoid hemorrhage显示文摘 | Sawauchi S Yuhki K Abe T | 2001 | NO Shinkei Geka2001，29，： | 1 |
| 17 | Decreased susceptibility to renovascular hypertension in mice lacking the prostaglandin I2 receptor IP显示文摘 | Fujino T Nakagawa N Yuhki K | 2004 | J Clin Invest2004，114，6： | 1 |
| 18 | BMPR1A signaling is necessary for hair follicle cycling and hair shaft differentiation in mice显示文摘 | Yuhki M Yamada M Kawano M | | 0，，08： | 1 |
| 19 | Molecular ge- netics and evolution of melanism in the cat family显示文摘 | Eizirik E Yuhki N Johnson W E | 2003 | CurrBio12003，13，5： | 1 |
| 20 | The relationship between delayed traumatic intracerebral hematoma and coagulopathy in patients diagnosed with a traumatic subarachnoid hemorrhage显示文摘 | Sawauchi S Yuhki K Abe T | 2001 | No ShinkeiGeka2001，29，2： | 1 |
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