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| 1 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 2 | Sequence modification of merB gene and high organomercurial resistance of transgenic tobacco plants显示文摘Mercury pollution has caused severe damage to environment and great attention has been paid to its control. Phytoremediation may become one of the most efficient measures to recover the polluted soil since it is economical, highly efficient and friendly to environment. In this report, plant genetic engineering methods were employed to modify the DNA sequence of merB genes that catalyze the conversion of organomercurals into ionic mercury. The modified mer-Bhe genes were introduced into tobacco by Agrobacterium, and the resultant transgenic plants were verified by Southern and Northern hybridization. High level of organomercu-rial resistance was detected on progenies of transgenic plants, some of which were resistant to PMA (phenyl mercury acetate) of 2.5μmol/L whereas 0.1μmol/L PMA killed the seedlings of wild-type tobacco in soiless culrure. With the increase of PMA concentration, the inhibition of the seedling growth became apparent. This result makes it possible to breed mercury-resistant tobacco | TIAN Jilin SHEN Ruijuan HE Yuke | 2002 | Chinese Science Bulletin2002，47，24： | 6 |
| 3 | Self-Sensing Test Method for the Temperature of Piezoelectric Stacks显示文摘A self-sensing test method for the temperature of piezoelectric stack,based on the high correlation between the static capacitance and the stack temperature,is proposed in order to construct a self-sufficient methodology of temperature measurement. Firstly,a theoretical model of static capacitance of the piezoelectric stack under preload was set up,and the influence of preload on the static capacitance was analyzed. Secondly,the correctness of the model was verified by static capacitance test experiments under various preloading conditions. Finally, the temperature measurement experiments at low-temperature stage for two kinds of piezoelectric stacks,namely the lowtemperature-resistant piezoelectric stack and conventional piezoelectric stack, were conducted under various preloading conditions using a polynomial fitting method. The results,which validate the accuracy of the test method,show that the maximum temperature deviations of the two kinds of piezoelectric stack are 3.9 ℃ and 2.8 ℃,respectively,when the preload force is close to the specified value. The test method uses the piezoelectric stack itself as a temperature sensor,which does not require additional equipment for temperature sensing,so that the space and equipment cost could be economized. And the test for static capacitance is concise and convenient,which indicates that in the cooling process,a concise and efficient test of the temperature of the piezoelectric stack could be realized so as to grasp the current temperature change in time. | SHEN Xing LI Yang LIANG Lei ZHANG Lei DAI Yuke CHEN Yuchen YANG Jinchuan | 2019 | Transactions of Nanjing University of Aeronautics and Astronautics2019，36，1： | 2 |
| 4 | HYL1 gene maintains venation and polarity of leaves显示文摘 | Lin Yu Xuhong Yu Ruijuan Shen Yuke He | 2005 | Planta2005，，2： | 1 |
| 5 | HYL1 gene maintains venation and polarity of leaves显示文摘 | Lin Yu Xuhong Yu Ruijuan Shen Yuke He | 2005 | Planta2005，，2： | 1 |
| 6 | The decoration of merB gene and high resistance of transgenic tobacco to organomercurials显示文摘 | TIAN Jielin SHEN Ruijuan HE Yuke | 2002 | Chinese Science Bulletin2002，47，23： | 1 |
| 7 | Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation显示文摘Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,respectively.Two significantly differentially expressed genes(Unigene13430_All and CL10096.Contig1_All)were associated with the auxin pathway.The full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length CL10096.Contig1_All coding sequence(1,470 bp)encoded 489 amino acids.Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19.The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant different.PsARF19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root formation.The results of this study may form the basis of future investigations on the mechanism mediating peony root formation.The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes. | Yinglong Song Wenqian Shang Zheng Wang Songlin He Xinya Meng Liyun Shi Yuxiao Shen Dan He Xueyuan Lou Yuke Sun | 2022 | Phyton-International Journal of Experimental Botany2022，91，12： | 0 |
| 8 | Role of metasomatized mantle lithosphere in the formation of giant lode gold deposits:Insights from sulfur isotope and geochemistry of sulfides显示文摘The Wulong deposit is one of the largest quartz vein-type gold deposits with at least 80 tons of identified gold reserves in the eastern part of the Liaodong Peninsula.Gold orebodies are mainly hosted in the Late Jurassic gneissic two-mica granite and Early Cretaceous diorite dykes,and are structurally controlled by the NNE-and NW-trending faults.Gold mineralization mainly occurs as veins with lenticular shapes and is closely associated with sulfides and Bi minerals.Previous studies on the deposit mainly focused on its geological characteristics,fluid inclusions and the timing of gold mineralization.However,the extreme enrichment mechanism of gold,the geodynamic setting and ore source of the gold deposit remain equivocal.Here we present results from geochemical studies of pyrite and pyrrhotite at Wulong to address these issues.Pyrrhotite can be grouped into euhedral(Po1)and anhedral(Po2)varieties based on the textures.Four generations of pyrite were identified based on petrographic studies:Py1 is coarse-grained subhedral to euhedral crystal coexisting with milky quartz,and Au-depleted in composition;Py2 is represented by medium-grained,subhedral to anhedral pyrite coexisting with euhedral Po1,smoky quartz and minor chalcopyrite;Py3 occurs as fined-grained anhedral grains in the quartz-polymetallic sulfide veins;Py4 occurs as veinlets or aggregates in the quartz-calcite veins and contains the lowest Au concentrations.The time-resolved LA-ICP-MS ablation depth profiles of sulfides combined with detailed petrological observations indicate that gold mainly occurs either as invisible solid solution within the crystal lattice of sulfides,to some extent,or as small blebs of native gold and electrum.The close temporal and spatial relationships among native bismuth,native gold and Bi-Te-S minerals suggest that Bi-rich melts may have significantly sequestered gold from the hydrothermal fluids in the Wulong gold deposit.Sulfides from auriferous quartz veins have a restricted D33S range(-0.2‰to+0.2‰)and a mean d34S value of+1.25‰with a feature of the mass-dependent fractionation(MDF)of sulfur isotopes,implying that the ore-forming materials were potentially derived from the mantle lithosphere that was metasomatized and fertilized by subduction-related fluids released from slab during the Early Cretaceous.This study demonstrates the Bi-rich melts as significant gold scavengers in As-deficient ore-forming fluid,and highlights the fundamental role of the metasomatized and fertilized mantle lithosphere in the formation of giant lode gold deposits in the eastern North China Craton at ca.120 Ma. | Baisong Du Zuoman Wang M.Santosh Yuke Shen Shufei Liu Jiajun Liu Kexin Xu Jun Deng | 2023 | Geoscience Frontiers2023，14，5： | 0 |
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