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| 1 | TALEN-based generation of a cynomolgus monkey disease model for human microcephaly显示文摘基因在非人类的首领编辑可以为在人探索病原学和遗传上基于的神经病学的混乱的治疗学的策略导致珍贵模型。然而,仔细在人模仿病理学、行为的赤字的神经病学的混乱的一个猴子模型成功地还没被产生了。Microcephalin 1 (MCPH1 ) 在人的大脑,和智力迟钝伴随的 MCPH1 变化原因头小畸型的进化被含有。这里,我们产生了用抄写带 biallelic MCPH1 变化的一只 cynomolgus 猴子(Macaca fascicularis ) 像使活跃之物的受动器核酸酶。猴子概括了大多数在病人观察的重要临床的特征,包括在头圆周,早熟的染色体冷凝作用(PCC ) ,语料库 callosum 的发育不全和上面的手足 spasticity 的显著减小。而且,在变异的真皮的成纤维细胞的 MCPH1 的 overexpression 救了 PCC 症候群。这个猴子模型可以帮助我们阐明在人的头小畸型并且更好的致病的 MCPH1 的角色在首领大脑尺寸的进化理解这蛋白质的功能。 | Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Jie Ren Xiaofeng Lin Haiqing Zheng Weijun Huang Yunhan Ma Dongdong Xu Zheng Chen Xinming Song Xinyi Lin Min Zhuang Tao Wang Fengfeng Zhuang Jianzhong Xi Frank Fuxiang Mao Huimin Xia Bruce T Lahn Qi Zhou Shihua Yang Andy Peng Xiang | 2016 | Cell Research2016，26，9： | 13 |
| 2 | The study of combustion synthesis of fine-particle γ-lithium aluminate显示文摘 | Wei Lin Xinde Bai Yunhan Ling Jinlong Yang Wenjun Ma | 2003 | Journal of Materials Science2003，，18： | 1 |
| 3 | PET image reconstruction with rotationally symmetric polygonal pixel grid based highly compressible system matrix显示文摘To achieve a maximum compression of system matrix in positron emission tomography (PET) image reconstruction, we proposed a polygonal image pixel division strategy in accordance with rotationally symmetric PET geometry. Geometrical definition and indexing rule for polygonal pixels were established. Image conversion from polygonal pixel structure to conventional rectangular pixel structure was implemented using a conversion matrix. A set of test images were analytically defined in polygonal pixel structure, converted to conventional rectangular pixel based images, and correctly displayed which verified the correctness of the image definition, conversion description and conversion of polygonal pixel structure. A compressed system matrix for PET image recon was generated by tap model and tested by forward-projecting three different distributions of radioactive sources to the sinogram domain and comparing them with theoretical predictions. On a practical small animal PET scanner, a compress ratio of 12.6:1 of the system matrix size was achieved with the polygonal pixel structure, comparing with the conventional rectangular pixel based tap-mode one. OS-EM iterative image reconstruction algorithms with the polygonal and conventional Cartesian pixel grid were developed. A hot rod phantom was detected and reconstructed based on these two grids with reasonable time cost. Image resolution of reconstructed images was both 1.35 mm. We conclude that it is feasible to reconstruct and display images in a polygonal image pixel structure based on a compressed system matrix in PET image reconstruction. | YU Yunhan XIA Yan CHEN Jing HONG Baoyu LIU Yaqiang WANG Shi MA Tianyu | 2013 | Nuclear Science and Techniques2013，24，3： | 0 |
| 4 | Rapid Quantitative Determination of Isoprene Monomer in Living Taraxacum kok-saghyz by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry显示文摘Taraxacum kok-saghyz(TKS)is rich in natural rubber(NR),a natural organic macromolecular compound composed of cis-1,4-polyisoprene,and may become the second NR-bearing plant for biochemical engineering development.In this paper,a rapid and quantitative ultra-high performance liquid chromatography tandem mass spectrometry(UHPLCMS/MS)method was established for determination of macromolecular biosynthesis substrate(dimethylallyl pyrophosphate,DMAPP)and initiator(farnesyl pyrophosphate,FPP)contained in TKS.A Kromasil C18 chromatographic column was used for separation,and the multi-reaction monitoring mode(MRM)of triple quadrupole mass spectrometry was used for detection.Quantification was performed by external calibration method.The results showed that the limit of detection(LOD)and the limit of quantitation(LOQ)of DMAPP were 2.42μg/L and 7.26μg/L,respectively,and the LOQ and the LOD of FPP were 1.02μg/L and 3.05μg/L,respectively.At a concentration of 1—1000μg/L,both analytes had good determination coefficients(>0.999)of calibration curve.The recoveries of DMAPP and FPP were between 99.0%and 117.1%.In real samples detection,the contents of DMAPP and FPP in TKS samples were between 23.32—82.77μg/L and 12.03—85.67μg/L,respectively.Thus,this approach is a reliable method to quantify DMAPP and FPP in TKS. | Xiang Tong Guo Tianyang Zhang Xi Chen Yunhan Dong Yiyang Zhang Jichuan Ma Qiang Zhang Liqun | 2020 | China Petroleum Processing & Petrochemical Technology2020，22，2： | 0 |
| 5 | Study on Apoptosis-Inducing Effect of XIAP Antisense Oligonucleotides on Glioblastoma Cells in Vitro显示文摘OBJECTIVE To investigate the apoptosis-inducing effect ofXIAP antisense oligonucleotides on glioblastoma cells in vitro.METHODS There were 4 groups in our experiment. Group A,as a cell control group, had normal cell culture and no treatmentapplied. Group B, as a blank control group, had normal cellculture and no liposome control of ASODN. Group C was N-ODN.Group D was the ASODN group. RT-PCR and Western blot assaywere conducted to detect the expression of XIAP in all A-172cell groups after treatment with XIAP antisense oligonucleotides(ASODN). MTT assay and flow-cytometry (FCM) detection wereused to detect the ability of cell anchoring growth and apoptoticrates of all groups. The processing time was 72 h.RESULTS The expression of XIAP in the A-172 cells was greatlydown-regulated, after treated with XIAP-ASODN. Amongdifferent concentrations of ASODN, the 300nM was the mostoptimal one. The down-regulation of XIAP obviously inhibited thesuccinate dehydrogenase (SDH) activity of the A-172 cells and theincreased apoptotic rate of A-172 cells (87.45%) was significantlyhigher than that of the A-172 in the control groups. There wasa statistically significant difference between the treatment andcontrol groups (P < 0.01).CONCLUSION The XIAP-ASODN can effectively regulate theexpression of the XIAP down, as a result, inhibit the growth of theglioblastoma cells (A-172) and obviously increase the apoptoticrate of the A-172 cells. The results of the study manifest an overtkilling role of XIAP-ASODN to the glioblastoma cells. | Zhongwei Zhao Zhengchun Sun Yunhan Zhang Ming Zhang Xudong Ma | 2009 | Chinese Journal of Clinical Oncology2009，6，2： | 0 |
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