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| 1 | Cloning and Expression Analysis of Citrus Genes Cs GH3.1 and Cs GH3.6Responding to Xanthomonas axonopodis pv. citri Infection显示文摘To study the functions of the early auxin-responsive genes Cs GH3.1 and Cs GH3.6 in citrus resistance against canker disease, we cloned Cs GH3.1and Cs GH3.6 in ‘Newhall' Navel Orange(Citrus sinensis Osbeck). They are 1 797 bp and 1 887 bp and encode 598 and 629 amino acids, respectively. In vitro mature leaves from susceptible ‘Newhall' and resistant Calamondin(C. madurensis) were inoculated by a Xanthomonas axonopodis pv. citri(Xac) bacterial suspension, and expression of Cs GH3.1 and Cs GH3.6 in the two varieties were analyzed using quantitative real-time PCR(q RT-PCR). ‘Newhall' leaves were treated with different hormones for 3 days, inoculated by Xac bacterial suspension, and then the symptoms in these leaves were investigated. We used q RT-PCR to analyze the effect of different hormones on Cs GH3.1 and Cs GH3.6 expression in ‘Newhall'leaves. The expression levels of both Cs GH3.1 and Cs GH3.6 were significantly induced by Xac in ‘Newhall' leaves, compared with levels in Calamondin leaves. 1-naphthy acetic acid(NAA) increased the hypertrophy of infection sites in ‘Newhall' leaves, while naphthyl-phthalamic acid(NPA)had no visible effect on lesion development. NAA hormone greatly improved expression of Cs GH3.1 in ‘Newhall', but not Cs GH3.6. These results indicate that the auxin primary-response gene Cs GH3.1 plays an important role in citrus susceptibility to Xac. | CHEN Min HE Yongrui XU Lanzhen PENG Aihong LEI Tiangang YAO Lixiao LI Qiang ZHOU Pengfei BAI Xiaojing DUAN Minjie JIANG Xueyou JIA Ruirui ZOU Xiuping CHEN Shanchun | 2016 | Horticultural Plant Journal2016，2，4： | 4 |
| 2 | Earth energy evolution, human development and carbon neutral strategy显示文摘Energy is the basis of human development and the impetus of society progress. There are three sources of energy: energy of celestial body outside the Earth, the Earth energy and energy of interaction between the Earth and other celestial bodies. Meanwhile, there are three scales of co-evolution: the evolution of the Sun-Earth-Moon system on an ultra-long time scale has provided energy sources and extra-terrestrial environmental conditions for the formation of the Earth system;the evolution of the Earth system on a long time scale has provided the material preconditions such as energy resources and suitable sphere environment for life birth and the human development;on a short time scale, the development of human civilization makes the human circle break through the Earth system, expanding the extraterrestrial civilization. With the co-evolution, there are three processes in the carbon cycle: inorganic carbon cycle, short-term organic carbon cycle and long-term organic carbon cycle, which records human immoderate utilization of fossil energy and global sphere reforming activities, breaking the natural balance and closed-loop path of the carbon cycle of the Earth, causing the increase of greenhouse gases and global climate change, affecting human happiness and development. The energy transition is inevitable, and carbon neutrality must be realized. Building the green energy community is a fundamental measure to create the new energy system under carbon neutrality target. China is speeding up its energy revolution and developing a powerful energy nation. It is necessary to secure the cornerstone of the supply of fossil energy and forge a strong growing pole for green and sustainable development of new energy. China energy production and consumption structure will make a revolutionary transformation from the type of fossil energy domination to the type of new energy domination, depending on a high-level self-reliance of science and technology and a high-quality green energy system of cleaning, low-carbon, safety, efficiency and independence. Energy development has three major trends: low-carbon fossil energy, large-scale new energy and intelligent energy system, relying on the green innovation, contributing the green energy and constructing the green homeland. | ZOU Caineng MA Feng PAN Songqi LIN Minjie ZHANG Guosheng XIONG Bo WANG Ying LIANG Yingbo YANG Zhi | 2022 | Petroleum Exploration and Development2022，49，2： | 1 |
| 3 | Cloning, expression, and purification of cold inducible RNA-binding protein and its neuroprotective mechanism of action显示文摘 | Jialin Liu Jinghui Xue Haitao Zhang Yuxiao Liu Minji Zou Donggang Xu Zhiwen Zhang | 2014 | Brain Research2014，，： | 1 |
| 4 | Global energy transition revolution and the connotation and pathway of the green and intelligent energy system显示文摘The essence of energy system transition is the'energy revolution':The development of the'resource-dominated'energy system with fossil energy as the mainstay has promoted human progress,but it has also triggered energy crisis and ecological environment crisis,which is not compatible with the new demands of the new round of scientific and technological revolution,industrial transformation,and sustainable human development.It is in urgent need to research and develop a new-type energy system in the context of carbon neutrality.In the framework of'technique-dominated'new green and intelligent energy system with'three new'of new energy,new power and new energy storage as the mainstay,the'super energy basin'concepts with the Ordos Basin,Nw China as a representative will reshape the concept and model of future energy exploration and development.In view of the'six inequalities'in global energy and the resource conditions of'abundant coal,insufficient oil and gas and infinite new energy'in China,it is suggested to deeply boost'China energy revolution',sticking to the six principles of independent energy production,green energy supply,secure energy reserve,efficient energy consumption,intelligent energy management,economical energy cost;enhance'energy scientific and technological innovation'by implementing technique-dominated'four major science and technology innovation projects',namely,clean coal project,oil production stabilization and gas production increasing project,new energy acceleration project,and green-intelligent energy project;implement'energy transition'by accelerating the green-dominated'four-modernization development',namely,fossil energy cleaning,large-scale new energy,coordinated centralized energy distribution,intelligent multi-energy management,so as to promote the exchange of two 80%s'in China's energy structure and construct the new green and intelligent energy system. | ZOU Caineng MA Feng PAN Songqi ZHAO Qun FU Guoyou ZHANG Guosheng YANG Yichao YU Hao LIANG Yingbo LIN Minjie WANG Ying XIONG Bo LIU Hanlin | 2023 | Petroleum Exploration and Development2023，50，3： | 0 |
| 5 | 人工智能白内障协同管理的通用平台显示文摘目的:建立和验证一个涉及多级临床场景的白内障协作通用的人工智能(artificial intelligence,AI)管理平台,探索基于AI的医疗转诊模式,以提高协作效率和资源覆盖率。方法:训练和验证的数据集来自中国AI医学联盟,涵盖多级医疗机构和采集模式。使用三步策略对数据集进行标记:1)识别采集模式;2)白内障诊断包括正常晶体眼、白内障眼或白内障术后眼;3)从病因和严重程度检测需转诊的白内障患者。此外,将白内障AI系统与真实世界中的居家自我监测、初级医疗保健机构和专科医院等多级转诊模式相结合。结果:通用AI平台和多级协作模式在三步任务中表现出可靠的诊断性能:1)识别采集模式的受试者操作特征(receiver operating characteristic curve,ROC)曲线下面积(area under the curve,AUC)为99.28%~99.71%);2)白内障诊断对正常晶体眼、白内障或术后眼,在散瞳-裂隙灯模式下的AUC分别为99.82%、99.96%和99.93%,其他采集模式的AUC均>99%;3)需转诊白内障的检测(在所有测试中AUC>91%)。在真实世界的三级转诊模式中,该系统建议30.3%的人转诊,与传统模式相比,眼科医生与人群服务比率大幅提高了10.2倍。结论:通用AI平台和多级协作模式显示了准确的白内障诊断性能和有效的白内障转诊服务。建议AI的医疗转诊模式扩展应用到其他常见疾病和资源密集型情景当中。 | WU Xiaohang HUANG Yelin LIU Zhenzhen LAI Weiyi LONG Erping ZHANG Kai JIANG Jiewei LIN Duoru CHEN Kexin YU Tongyong WU Dongxuan LI Cong CHEN Yanyi ZOU Minjie CHEN Chuan ZHU Yi GUO Chong ZHANG Xiayin WANG Ruixin YANG Yahan XIANG Yifan CHEN Lijian LIU Congxin XIONG Jianhao GE Zongyuan WANG Dingding XU Guihua DU Shaolin XIAO Chi WU Jianghao ZHU Ke NIE Danyao XU Fan LV Jian CHEN Weirong LIU Yizhi 林浩添 无 王厚硕(审校) 罗明杰(审校) | 2023 | 眼科学报2023，38，10： | 0 |
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