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| 1 | Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster ( Pinctada fucata , Gould 1850) responding to bacterial challenge显示文摘 | Zhongliang Wang Zaohe Wu Jichang Jian Yishan Lu | 2008 | Fish and Shellfish Immunology2008，，4： | 2 |
| 2 | Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson)显示文摘 | Jichang Jian Zaohe Wu | 2003 | Aquaculture2003，218，14： | 1 |
| 3 | Cloning and expression of the gene encoding an extracellular alkaline serine protease from Vibrio alginolyticus strain Hy9901, the causative agent of vibriosis in Lutjanus erythopterus (Bloch) 显示文摘 | CAI Shuanghu WU Zaohe JIAN Jichang | 2007 | Journal of Fish Diseases2007，30，8： | 1 |
| 4 | Cloning andexpression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850 ) responding to bacterial challenge 显示文摘 | WANG Zhongliang WU Zaohe JIAN Jichuang | 2009 | Fish and Shellfish Immunology2009，26，4： | 1 |
| 5 | GCRV 096 VP6 protein and its impacts on GCRV replication with different genotypes in CIK cells显示文摘Grass carp reovirus(GCRV)is the causative agent of grass carp hemorrhagic disease,which has detrimental effects on the grass carp aquaculture industry.There are four known genotypes of GCRV and strains of different GCRV genotypes differ greatly.In this study,the diversity of the protein VP6 from different GCRV stains and the effect of genotype GCRV 096 on replication was investigated in CIK cells.Our results showed that the VP6 protein of GCRV 096(genotypeІ)exhibited limited homology to that of GCRV GD108(genotypeШ),with few residues conserved in predicted protein-protein interaction domains.GCRV 096 VP6 protein was expressed and purified and an antiserum against it was characterized.Addition of purified VP6 protein or antiserum to culture media of CIK cells inhibited the replication of GCRV 096 in these cells.In contrast,replication of GCRV GD108(genotypeШ)was not affected in CIK cells under the same condition.Overall,our results indicated that the protein VP6 and VP6 antiserum did not provide cross-protection against GCRV strains and this can be attributed to differences among GCRV genotypes.It will be important to consider multiple GCRV genotypes in the development of effective GCRV vaccines and other therapies against grass carp hemorrhagic disease.In addition,bioinformatics analysis also suggested that the protein VP6 may have a role in the process of GCRV infection.This study lays the foundation for the prevention of grass carp hemorrhagic disease and further detailed studies on the pathogenesis of GCRV. | Xiuying Yan Lingfang Xiong Jie Li Ya Wang Zaohe Wu Jichang Jian Yu Ding | 2018 | Aquaculture and Fisheries2018，3，5： | 1 |
| 6 | Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea(Richardson) 显示文摘 | Jichang Jian Zaohe Wu | 2003 | Aquaculture2003，218，14： | 1 |
| 7 | Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson) 显示文摘 | Jichang Jian Zaohe Wu | 2003 | Aquacuhure2003，218，14： | 1 |
| 8 | 4-(2,2': 6', 2'-Terpyridyl) benzyltriphenylphosphonium hromide显示文摘 | SONG Wendong HONG Pengzhi WU Zaohe | 2007 | Aeta Crystallography Section E2007，63，： | 1 |
| 9 | Isotopic niche overlap of two planktivorous fish in southern China显示文摘 | CHEN Gang WU Zaohe GU Binhe | 2011 | Limnology2011，12，2： | 1 |
| 10 | Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena croce (Richardson) 显示文摘 | JIAN Jichang Wu Zaohe | 2003 | Aquaculture2003，218，14： | 1 |
| 11 | Construction of Live Attenuated Vaccine against Vib- rio alginolyticus and the Evaluation on Its Immuno- protection Effect显示文摘Vibrio alginolyticus,a gram-negative bacterium has been described to be one of the most common and economically important aquatic pathogens of fish and shellfish. Vaccine immunization is an effective approach preventing V. alginolyticus infection. Attenuated vaccine stimulates systemic immune response in the host,but few attenuated vaccine against V. alginolyticus is available. The type III secretion system( T3SS),an important pathogenic factor of V. alginolyticus,is used by bacterial pathogens to inject effector proteins into the cytoplasm of their host cells. The T3 SS forms a structure called needle complex with a multi-ring base that spans the bacteria and a needle-like extension that protrudes several nanometers from the bacterial surface. vscO locates at the ' needle' site of T3 SS,playing the role of escorting the molecular chaperone and effector proteins into host cells and further inducing the death of host cells. In this paper,an in-frame deletion mutant of vscO was constructed using overlap PCR and homologous recombination technology combining with chloramphenicol( Cm) and sucrose screening. The LD50 changes of ZJ03ΔvscO mutant strains compared with the strain ZJ03 were examined in grouper( Epinephelus coioides). The ZJ03ΔvscO mutant showed about 150 times decrease in virulence in E. coioides compared with wide type ZJ03. After vaccination with ZJ03ΔvscO in E. coioides through injection and immersion,the specific antibody titers were markedly higher than that in the saline control group( P < 0. 05). The titers of injection and immersion group on the forth week reached the maximums at 1∶ 2 048 and 1∶ 128,respectively. The relative percentage survival( RPS) of injection group was 84%,while that in immersion group was 68%.These results indicate that the ZJ03ΔvscO of V. alginolyticus has a high immunogenicity,and can be used as live attenuated vaccine. In addition,RPS may be affected by vaccination and infection methods. This study can provide technical support for controlling fish diseases caused by V. alginolyticus. | Yuan Yonghong Pang Huanying Zhou Zejun Song Dawei Ding Yu Jian Jichang Wu Zaohe | 2016 | Animal Husbandry and Feed Science2016，8，2： | 0 |
| 12 | Construction and Biological Function Analysis of a dldh Deletion Mutant Strain of Vibrio alginolyticus显示文摘[Objective] This study aimed to investigate the role of dihydrolipoamide dehydrogenase( DLDH) in the pathopoiesis of Vibrio alginolyticus. [Method] Using suicide plasmid pRE112 as the vector,dldh deletion mutant strain ZJ03Δdldh was constructed with insertion inactivation mutation method by Overlap PCR and homologous recombination technology to compare and analyze the differences in the growth,swimming ability,enzyme activity,biofilm formation and pathogenicity between wild-type strain ZJ03 and deletion mutant strain ZJ03Δdldh. [Result]dldh deletion mutant strain ZJ03Δdldh exhibited prolonged lag phase,significantly reduced swimming ability( P < 0. 05),significantly reduced enzyme activity( P < 0. 05),significantly reduced biofilm formation ability( P < 0. 05) and remarkably reduced pathogenicity to Epinephelus coioides( P < 0. 05) compared to wild-type strain ZJ03. [Conclusion] This study provided theoretical basis for revealing the pathogenic mechanisms of Vibrio from the perspective of bacterial energy metabolism. | Huanying PANG Liming CHEN Yanfei ZHANG Jing LI Mingsheng QIU Jichang JIAN Zaohe WU | 2015 | Agricultural Biotechnology2015，4，4： | 0 |
| 13 | Molecular Cloning,Bioinformatics Analysis and Expression Analysis of Type III Secretion System(T3SS)Injectisome Gene vscX from Vibrio alginolyticus显示文摘In order to enrich the research about type Ⅲ secretion system(T3SS) injectisome of Vibrio alginolyticus,vscX gene was cloned from V.alginolyticus strain HY9901 for bioinformatics analysis and expression analysis.Specific primers were designed according to the full-length genome sequence of V.alginolyticus in GenBank.vscX gene(GenBank accession number:FR780679) contained a 378 bp open reading frame(ORF),encoding a putative protein of 125 amino acids.The theoretical molecular weight was 14.209 2 kD and theoretical pI was 5.75.By using Signal 4.1 Server and TMHMM Server 2.0,it was predicted that VscX protein had no transmembrane domain or signal peptide.The results of prediction using SoftBerry-Psite software showed that VscX protein contained three casein kinase Ⅱ phosphorylation sites,one N-myristoylation site and three C-terminal targeting signal sites.Subcellular localization revealed that VscX might be located in cytoplasm with the possibility of 56.5%.According to SMART prediction,VscX had one Pfam(1-125 aa) domain.Phylogenetic analysis revealed that VscX from V.alginolyticus and VscX from V.parahemolyticus were clustered into the same group.Network interaction analysis showed that vscX was adjacent to vscY,vopB and sycN.By real-time fluorescent quantitative PGR technique and 2^(-△△Ct) method,the differences in expression levels of VscX mRNA in V.alginolyticus strain HY9901,T3 SS deletion strain AvscO and complementary strain C-vscO at different growth stages were analyzed.The results showed that the expression levels of VscX mRNA in V.alginolyticus strain HY9901 and C-vscO were significantly up-regulated at stable growth stage(P<0.01);the expression levels of VscX mRNA in deletion strain AvscO were significantly up-regulated at late growth stage(P <0.01).This study provided the basis for revealing the transport mechanism of T3 SS injectisome of V.alginolyticus. | Xieyan CHEN Jing LI Huanying PANG Yunsheng CHANG Yucong HUANG Zaohe WU Jichang JIAN | 2017 | Agricultural Biotechnology2017，6，1： | 0 |
| 14 | Characterization and Expression Analysis of B Cell Receptor Accessory Molecule CD79 Gene in Humphead Snapper(Lutjanus sanguineus)显示文摘CD79,a key component of the B cell antigen receptor complex,is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively,and plays an important role in B cell signaling.In this study,we isolated and characterized mb-1 and B29 from humphead snapper(Lutjanus sanguineus).Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated.The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp,encoding 227 amino acids and 201 amino acids,respectively.Both CD79α and CD79β possess signal peptide,extracellular Ig domain,transmembrane region and immunoreceptor tyrosine kinase activation motif(ITAM).Mb-1 is highly expressed in lymphoid organs(thymus,posterior kidney and spleen) and mucosal-associated lymphoid tissues(gill and intestine),while B29 is mainly detected in posterior kidney,spleen,gill and skin.Furthermore,transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide(LPS),pokeweed mitogen(PWM),and polyinosinic-polycytidylic acid(Poly I:C) stimulation,respectively,and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi.These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection. | HUANG Yucong YANXiuying CAI Shuanghu CAI Jia JIAN Jichang LU Yishan 3,4TANG Jufen WU Zaohe | 2016 | Journal of Ocean University of China2016，15，2： | 0 |
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