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| 1 | Analytical validation of GMEX rapid point- of care CYP2C19 genotyping system for the CHANCE-2 trial显示文摘Background and purpose Rapid genotyping is useful for guiding early antiplatelet therapy in patients with high-risk nondisabling ischaemic cerebrovascular events(HR-NICE).Conventional genetic testing methods used in CYP2C19 genotype-guided antiplatelet therapy for patients with HR-NICE did not satisfy the needs of the Clopidogrel in High-Risk Patients with Acute Nondisabling Cerebrovascular Events(CHANCE)-2 trial.Therefore,we developed the rapid-genotyping GMEX(point-of care)system to meet the needs of the CHANCE-2 trial.Methods Healthy individuals and patients with history of cardiovascular diseases(n=408)were enrolled from six centres of the CHANCE-2 trial.We compared the laboratory-based genomic test results with Sanger sequencing test results for accuracy verification.Next,we demonstrated the accuracy,timeliness and clinical operability of the GMEX system compared with laboratory-based technology(YZY Kit)to verify whether the GMEX system satisfies the needs of the CHANCE-2 trial.Results Genotypes reported by the GMEX system showed 100%agreement with those determined by using the YZY Kit and Sanger sequencing for all three CYP2C19 alleles(*2,*3 and*17)tested.The average result’s turnaround times for the GMEX and YZY Kit methods were 85.0(IQR:85.0-86.0)and 1630.0(IQR:354.0-7594.0)min(p<0.001),respectively.Conclusions Our data suggest that the GMEX system is a reliable and feasible point-of care system for rapid CYP2C19 genotyping for the CHANCE-2 trial or related clinical and research applications. | Xia Meng Anxin Wang Guojun Zhang Siying Niu Wei Li Sifei Han Fang Fang Xingquan Zhao Kehui Dong Zening Jin Huaguang Zheng Kelin Chen Hao Li Chengyuan Yang Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 7 |
| 2 | Trends and predictors of myocardial infarction or vascular death after ischaemic stroke or TIA in China, 2007-2018: insights from China National Stroke Registries显示文摘Background Although stroke management,primary and secondary preventions have been improved in China last decades,the trends and predictors of major vascular events after ischaemic stroke or transient ischaemic attack(TIA)at national scale are less known.Methods Data were obtained from the three phases of China National Stroke Registry(CNSR),including CNSR-Ⅰ(years 2007-2008),CNSR-Ⅱ(years 2012-2013)and CNSR-III(years 2015-2018).For comparison,patients who were diagnosed as ischaemic stroke or TIA were included.Kaplan-Meier estimates of myocardial infarction(MI)or vascular death were calculated at 1 year.Independent predictors were further assessed with a Cox proportional hazards regression.Results From 2007 to 2018,a total of 50284 patients with ischaemic stroke or TIA were enrolled in this study.A declining trend was found in 1-year MI or vascular death(p for trend<0.001),while recurrent stroke depicted a U-shape curve with a nadir in 2012-2013 cohort.A similar trend was also observed in patients who were admitted to 26 hospitals in all three CNSRs.In 2015-2018 cohort,only 251(1.7%;95%CI 1.5%to 1.9%)MI or vascular death had occurred at 1 year.Older age,previous stroke or TIA,history of coronary artery disease and the National Institutes of Health Stroke Scale>6 were associated with both an increased risk of MI or vascular death and recurrent stroke.While early antiplatelet therapy and lipid-lowering agents at discharge predicted a reduced risk.Conclusion A declining trend and current low incidence of MI or vascular death,rather than recurrent stroke,after ischaemic stroke or TIA were observed in China.Traditional factors were found as independent predictors.These findings suggested there is still much room to improve for stroke management. | Long Li Yuesong Pan Mengxing Wang Jing Jing Xia Meng Yong Jiang Caixia Guo Zening Jin Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 4 |
| 3 | Inhibition of the lncRNA DANCR attenuates cardiomyocyte injury induced by oxygen-glucose deprivation via the miR-19a-3p/MAPK1 axis显示文摘Long noncoding RNAs(lncRNAs)have been considered as crucial regulators of acute myocardial infarction(AMI).In this study,to analyze the effect of differentiation antagonizing nonprotein coding RNA(DANCR)of lncRNA on cardiomyocyte damage in AMI,cardiomyocyte injury was induced by oxygen-glucose deprivation(OGD).Cell counting kit-8(CCK-8)assay and flow cytometry were used to assess cell viability and apoptosis,respectively.Quantitative real-time PCR was used to measure the expression levels of DANCR and miR-19a-3p.Bioinformatics analysis and luciferase gene reporter assay were utilized to explore the relationship among DANCR,miR-19a-3p,and mitogen-activated protein kinase 1(MAPK1).CCK-8 and TUNEL assays were used to explore the effects of DANCR alone or plus miR-19a-3p on the viability and apoptosis of OGD/R-exposed HL-1 cells.Western blot analysis was used to detect changes in the MAPK1/ERK1/2 pathway in HL-1 cells.We found that DANCR expression and miR-19a-3p level are negatively correlated as DANCR expression is increased,while miR-19a-3p level is decreased in AMI patients’serum and OGD/R-exposed HL-1 cells.DANCR knockdown increased miR-19a-3p level,and miR-19a-3p inhibition increased DANCR expression.Moreover,DANCR directly binds to miR-19a-3p.DANCR knockdown reduced viability but induced apoptosis in OGD/R-exposed HL-1 cells,while miR-19a-3p inhibition weakens these effects.Furthermore,MAPK1 is a target of miR-19a-3p.miR-19a-3p overexpression decreases MAPK1 and ERK1/2 in HL-1 cells,while miR-19a-3p inhibition increases MAPK1 and ERK1/2 in HL-1 cells.Moreover,DANCR knockdown reduces myocardium apoptosis in mice with the left anterior descending artery ligated.DANCR knockdown effectively restores myocardial cell apoptosis by regulating the miR-19a-3p/MAPK1/ERK1/2 axis. | Yang Ruan Hong Li Xiaojing Cao Shuai Meng Ruofei Jia Lianmei Pu Hao Fu Zening Jin | 2021 | Acta Biochimica et Biophysica Sinica2021，53，10： | 4 |
| 4 | Framework and Key Technologies of Human-machine Hybrid-augmented Intelligence System for Large-scale Power Grid Dispatching and Control显示文摘With integration of large-scale renewable energy,new controllable devices,and required reinforcement of power grids,modern power systems have typical characteristics such as uncertainty,vulnerability and openness,which makes operation and control of power grids face severe security challenges.Application of artificial intelligence(AI)technologies represented by machine learning in power grid regulation is limited by reliability,interpretability and generalization ability of complex modeling.Mode of hybrid-augmented intelligence(HAI)based on human-machine collaboration(HMC)is a pivotal direction for future development of AI technology in this field.Based on characteristics of applications in power grid regulation,this paper discusses system architecture and key technologies of human-machine hybrid-augmented intelligence(HHI)system for large-scale power grid dispatching and control(PGDC).First,theory and application scenarios of HHI are introduced and analyzed;then physical and functional architectures of HHI system and human-machine collaborative regulation process are proposed.Key technologies are discussed to achieve a thorough integration of human/machine intelligence.Finally,state-of-theart and future development of HHI in power grid regulation are summarized,aiming to efficiently improve the intelligent level of power grid regulation in a human-machine interactive and collaborative way. | Shixiong Fan Jianbo Guo Shicong Ma Lixin Li Guozheng Wang Haotian Xu Jin Yang Zening Zhao | 2024 | CSEE Journal of Power and Energy Systems2024，10，1： | 0 |
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