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| 1 | Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats显示文摘AIM: To invsstigare the effect of L-NAME on nitric oxide andgastriubtestubal motility alterations in cirrhotic ratsMETHODS: Rats with cirrhosis induced by carbontetrachloride were randomly divided into two groups, one( n= 13) receiving 0. 5 mg@ kg-1 per clay of NG-nitro-L-argininemethyl ester (L-NAME), a nitric oxide synthase inhibitor,for 10 days, whereas the other group ( n = 13) and control( n = 10) rats were administrated the same volume of 9 g@ L-1saline.Half gastric emptying time and 2 h residual rate weremeasured by SPECT, using 99m Tc-DTPA-labeled bariumsuifate as test meal. Gastrointestinal transition time wasrecorded simultaneously. Serum concentration of nitrcoxide (NO) was determined by the kinetic cadmiunreduction and colorimetric methods. ImmunohistochemicalSABC method was used to observe the expression anddistribution of three types of nitric oxide synthase (NOS)isoforms in the mt gastrointestinal tract. Western blot wasused to detect expression of gastrointestinal NOS isoforms.RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged( 124.0 ± 26.4min; 33.7± 8.9min;72.1 ± 15.3 min; P<0.01), (12.4±0.5h; 9.5±0.3 h; 8.2±0.8 h; P<0.01), 2h residual rate wasraised in cirrhotic rots than in controls and cirrhotic ratstreated with L-NAME(54.9± 7.6 % ,13.7 ± 3.2 %, 34.9± 10.3%, P< 0.01). Serum concentration of NO was significantlyincreased in cirrhotic rots than in the other groups (8.20 ± 2.48)μmol@L-1, (5.94± 1.07) μmol@L-1 ,and control (5.66± 1.60) tμmol@L-1, P< 0.01. NOS staining intensities which weremainly located in the gastrointestinal tissues were markedlylower in cirrhotic rats than in the controls and cirrhotic ratsafter treated with L- NAME.CONCLUSION: Gastrointestinal motility was remarkablyinhibited in cirrhotic rats, which could he alleviated by L-NAME. Nitric oxide may play an important role in theinhibition of gastrointestinal motility in cirrhotic rats. | Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,Institute of Digestive disease,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Yue-Xia Zhong,Emergency Department,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,Shaanxi Province,China Ju Lu,Class EE 87,Department of Electronic Engineering,Tsinghua University,Beijing 100084,China Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 2 | Nat Chem Biol:利用CRISPR-Cas9激活细菌中沉默的基因簇,有望发现新的药物显示文摘为了抵抗疾病,很多医药库中的武器是从细菌当中获得的。如今,利用CRISPR-Cas9基因编辑技术。研究人员揭示出沉默基因中隐藏着的更多潜在的宝藏。作为一类常见的细菌。链霉菌被用来产生很多作为抗生素、抗癌试剂和其他药物的化合物。在一项新的研究中。来自美国伊利诺伊大学和新加坡科技研究局的研究人员利用CRISPR-Cas9技术激活链霉菌中不表达的或者说沉默的基因簇。 | Mingzi M Zhang, Wan Lin Yeo, Ee Lui Ang, Huimin Zhao Fong Tian Wong, Elena Heng Yajie Wang, Shangwen Luo, Ryan E Cobb, Behnam Enghiad, Huimin Zhao Yee Hwee Lim | 2017 | 现代生物医学进展2017，17，17： | 12 |
| 3 | Infl uence of nitrate concentrations on EH40 steel corrosion aff ected by coexistence of Desulfovibrio desulfuricans and Pseudomonas aeruginosa bacteria显示文摘Nitrate addition is a common bio-competitive exclusion(BCE)method to mitigate corrosion in produced water reinjection systems,which can aff ect microbial community compositions,especially nitrate and sulfate reducing bacteria,but its eff ectiveness is in controversy.We investigated the infl uence of nitrate concentrations on EH40 steel corrosion aff ected by coexistence of Desulfovibrio vulgaris and Pseudomonas aeruginosa bacteria.Results demonstrate that only mixed bacteria or nitrate had little eff ect on EH40 steel corrosion,and nitrate could accelerate the corrosion of EH40 steel through the action of microorganisms.The corrosion promotion of nitrate was dependent on its concentrations,which increased from 0 to 5 g/L and decreased from 5 to 50 g/L.These diff erences were believed to be related to the regulation of nitrate in the growth of bacteria and biofi lms.Therefore,care must be taken to BCE method with nitrate when nitrate reducing bacteria with high corrosive activity are present in the environments. | Zhihua SUN Jiajia WU Dun ZHANG Ce LI Liyang ZHU Ee LI | 2022 | Journal of Oceanology and Limnology2022，40，4： | 2 |
| 4 | Protein and RNA expression of acid-sensing ion channels 2 and 3 in myocardial ischemia rats induced by isoproterenol显示文摘OBJECTIVE:To investigate the impact of electro-acupuncture at the Neiguan(PC 6) acupoint on protein and RNA expression of acid-sensing ion channel 2(ASIC2) and ASIC3 in myocardial ischemia rats.METHODS:Fifty male Sprague-Dawley rats were used,weighing(230 ± 50) g.The rats were randomized into a normal group A,model group B,Neiguan(PC 6) group C,Lieque(LU 7) group D,and A-shi points group E.There were 10 rats in each group.Rats were continuously administered 85 mg/kg intravenous isoproterenol daily to establish the model.Successfully modeled rats in groups C,D,and E were given electro-acupuncture treatment.Each group of rats was sacrificed with chloral hydrate(1 mL/100 g) intraperitoneal injection.The left ventricular myocardium was extracted and placed at- 70 ℃ until use.Western blot analysis and real-time PCR were performed to assay protein and RNA expressions of ASIC2 and ASIC3,respectively.Fold changes in RNA expression were quantified with the 2~^(-△△Ct) method.Blood samples were drawn from the aorta abdominalis and tested for creatine kinase-MB(CK-MB) and lactate dehydrogenase(LDH) levels using enzyme-linked immunosorbent assay.RESULTS:Myocardial ischemia rats given electro-acupuncture at the Neiguan(PC 6) acupoint had significantly lower protein and RNA expression of ASIC2 and ASIC3,and CK-MB and LDH levels,compared with model rats(P < 0.01).CONCLUSION:Electro-acupuncture at the Neiguan(PC 6) acupoint can not only decrease the protein and RNA expression of ASIC2 and ASIC3,but also inhibit the opening of ASICs and reduce the cardiomyocyte damage in myocardial ischemia rats. | Wang Shudong Zhang Lide Dong Baoqiang Zhang Wenshun Ho Chin Ee Guo Na Chen Yiguo | 2015 | Journal of Traditional Chinese Medicine2015，35，2： | 2 |
| 5 | Inducible nitric oxide synthase,nitrotyrosine,and apoptosis in Helicobacter pylori gastritis:effect of antibiotics and antioxidants 显示文摘 | Mannick EE Bravo LE Zarama G Realpe JL Zhang XJ Ruiz B Eonthan ETH Mera R Miller MJL Correa P | 1996 | Cancer Res1996，56，： | 1 |
| 6 | Variability oi' renal apparent diffusion coefficients:limitaions of the monocx- ponetial model for diffusion quantification 显示文摘 | Zhang JL Sigmund EE Chandarana H | 2010 | Radiology2010，254，： | 1 |
| 7 | Cryptochrome mediates circadian regulation of cAMP signaling and hepatic gluconeogenesis显示文摘 | Zhang EE Liu Y | 2010 | Nature Medicine2010，16，10： | 1 |
| 8 | Cyclopiazonic acid增加SHR和WKY血管平滑肌细胞Ca^(2+)-依赖性外向K^+-电流显示文摘采用全细胞及细胞贴附式斑片钳技术记录自发性高血压大鼠(SHR)和Wistar-Kyoto对照鼠(WKY)培养主动脉平滑肌细胞的Ca^(2+)-依赖性外向K^+电流[I_(k(Ca))],测定肌浆网Ca^(2+)泵抑制剂CPA对其影响.CPA能增加I_K(Ca))单通道开放时间,缩短关闭时间,增加全细胞I_(K(Ca))幅度,这些作用与Ca^(2+)相关并可被K^+通道阻断药glybenclamide阻断。CPA作用在SHR和WKY之间无明显差异。结果提示高血压状态下血管平滑肌的功能改变可能与I_(K(Ca))无关。 | 关永源 John Zhang Lee RMKW Kwan CY Daniel EE | 1993 | 中国药理学通报1993，9，4： | 1 |
| 9 | The role of beta-catenin,TGF beta 3,HGF2,FGF2,IGFR2,and BMP4 in the pathogenesis of mesenteric sclerosis and angipathy in midgut carcinois显示文摘 | Zhang PJ Furth EE Cai X | 2004 | Hum Pathol2004，35，6： | 1 |
| 10 | Femtosecond laser versus mechanical microkeratome laserin situ keratomileusis for myopia : Metaanalysis of randomizedcontrolled trials 显示文摘 | Zhang ZH Jin HY Suo Y Patel SV Montes-Mlco R Manche EE | 2011 | J Cataract Refract Surg2011，3，： | 1 |
| 11 | A genome-wide RNAi screen for modifiers of the circadian clock in human cells显示文摘 | Zhang EE Liu AC Hirota T | | 0，，01： | 1 |
| 12 | Development and validation of a C0-C7 FE complex for biomechanical study显示文摘 | QING HANG ZHANG EE CHON TEO HONG WAN NG | 2005 | Journal of Biomechanical Engineering2005，127，5： | 1 |
| 13 | Thyroid hormoneβreceptor activation has additive cholesterol lowering activity in combinatiowith atorvastatin in rabbits,dogs and monkeys显示文摘 | Ito BR Zhang BH Cable EE | 2013 | Br J Pharmacol2013，156，3： | 1 |
| 14 | Antitumor efficiency of the cytosine deaminase/5 fluorocytosine suicide gene therapy system on malignant gliomas: an in vivo study 显示文摘 | Lv SQ Zhang KB Zhang EE | 2009 | Med Sci Monit2009，15，1： | 1 |
| 15 | Advances in systems biology are en- hancing our understanding of disease and moving us closer to no- vel disease treatments 显示文摘 | Sehadt EE Zhang B Zhu J | 2009 | Genetiea2009，136，2： | 1 |
| 16 | TTC21B contributes both causal and modifying alleles across the ciliopathy spectrum显示文摘 | Davis EE Zhang Q Liu Q | | 0，，03： | 1 |
| 17 | Mutational dynamics of the SARS coronavirus in cell culture and human populations iso- lated in 2003 显示文摘 | Vega VB Ruan Y Liu J Lee WH Wei CL Se-Thoe SY Tang KF Zhang T Kolatkar PR Ooi EE Ling AIE Stan- ton LW Long PM Liu ET | 2004 | BMC Infect Dis2004，4，： | 1 |
| 18 | TTC21B contributes both causal and modifying alleles across the ciliopathy spectrum显示文摘 | Davis EE Zhang Q Liu Q | 2011 | Nat Genet2011，43，3： | 1 |
| 19 | Dimerization of the human papillomaviros type 16 E2 N terminus results in DNA looping within the upstream regulatory region显示文摘 | Hernandez-Ramon EE Burns JE Zhang W | 2008 | J Virol2008，82，10： | 1 |
| 20 | Optimization of b-value sampling for diffusion-weighted imaging of the kidney 显示文摘 | Zhang JL Sigmund EE Rusinek H | 2012 | Magn Reson Med2012，67，1： | 1 |
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