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| 1 | Efficient genome editing in plants using a CRISPR/Cas system显示文摘 | Zhengyan Feng Botao Zhang Wona Ding Xiaodong Liu Dong-Lei Yang Pengliang Wei Fengqiu Cao Shihua Zhu Feng Zhang Yanfei Mao Jian-Kang Zhu | 2013 | Cell Research2013，23，10： | 211 |
| 2 | Orthogonal analysis of water model study on the optimization of flow control devices in a six-strand tundish显示文摘Improper flow control devices in a multi-strand tundish can cause some problems, for example, liquid steel cannot reach every nozzle at the same time and the liquid steel in nozzles far away from the entry zone has a lower temperature. The water model experiment of a six-strand tundish of Tianjin Iron & Steel Co. Ltd. was performed, a new 'U' type baffle was obtained, and its parameters were defined by perpendicular analysis. The 'U' baffle can not only improve those imperfections, but also prolong the residence time of nonmetallic inclusions, which is good for their flotation and separation. | Zhengyan Wei Yanping Bao Jianhua Liu Wenxu Gong Baoming Wang | 2007 | Journal of University of Science and Technology Beijing2007，14，2： | 18 |
| 3 | Bulking factor of the strata overlying the gob and a three-dimensional numerical simulation of the air leakage flow field显示文摘现在的学习检验与在家和国外执行的嘴 bulking 因素有关的研究的结果。一个三维的嘴 bulking 因素的数学功能基于一个三维的嘴模型被描述。为空隙和渗透比率拿价值的方法也被建议。充分使机械化的最高的煤的空气漏的法律在这研究被研究。结果证明上面、更低的 crossheadings 附近的空气流动的速度在在从工作的脸的一样的距离的嘴的中央节比那高。当在工作的脸的空气的数量超过批评数量时,在上面、更低的 crossheadings 的自燃地区的宽度在中央节也比那大。处于这种状况,钥匙正在阻止从 self-igniting 在上面、更低的 crossheadings 离开的煤。在工作的脸减少空气的数量能减少自燃地区的宽度,特别上面、更低的 crossheadings 附近的宽度。这也在工作的脸的方向移动自燃地区。它能由在惰性的 crossheadings 使煤左并且由有效地在工作的脸控制空气的数量在嘴阻止煤 self-igniting。 | Shao Hao Jiang Shuguang Wang Lanyun Wu Zhengyan | 2011 | Mining Science and Technology2011，21，2： | 16 |
| 4 | Oxidation kinetics regularity in spontaneous combustion of gas coal显示文摘In order to investigate the oxidation kinetics of gas coal at low temperatures, we derived a rate equation of oxygen con-sumption during low-temperature oxidation of gas coal and deduced an E-c equation, expressing the relation between active energy E and oxygen concentration c. The reaction order n and active energy E were calculated with this equation based on experiments of static oxygen consumption tests. In addition, we proved the rationality of the E-c equation using a kinetic compensation effect and obtained the isokinetic temperature Tc. The results show that: 1) the gas coal oxidizes easily with increasing temperature and the oxidation tends to be spontaneous at higher temperatures; 2) the oxygen concentration c affects oxygen consumption very much at lower temperatures but has only a small effect at higher temperatures; 3) the isokinetic temperature Tc was 127 °C which has been experimentally validated as the key turning point during low-temperature spontaneous combustion of gas coal. | WANG Lanyun JIANG Shuguang WU Zhengyan SHAO Hao ZHANG Weiqing CHEN Yueqin ZOU Lili | 2010 | Mining Science and Technology2010，20，1： | 13 |
| 5 | Spatial distribution and deposition history of nonylphenol and bisphenol A in sediments from the Changjiang River(Yangtze River) Estuary and its adjacent East China Sea显示文摘Nonylphenol(NP) and bisphenol A(BPA) are endocrine disruptors causing harmful effects including feminization and carcinogenesis to various organisms,and consequently,their contamination in natural environment has received wide concerns.This study reports the distribution characteristics of NP and BPA in surface sediments and their deposition history based on a dated sediment core in the Changjiang River(Yangtze River) Estuary and its adjacent East China Sea.The contents of NP and BPA in surface sediments ranged from 1.56-35.8 and 0.72-13.2 ng/g(dry mass),respectively,with high values recorded in the two mud zones,the Changjiang River Estuarine Mud Zone and the Zhejiang Coastal Mud Zone.High values in the Zhejiang Coastal Mud Zone suggest the possibility of long distance transport of both contaminants through the Changjiang riverine plume.The contents were not correlated with the distance from the pollution source,indicating other factors including particle deposition rate and sediment grain size obviously affecting the distribution pattern.NP was also detected in a sediment core at layers deposited from the year of 1971 to 2001 with contents of up to 20.9 ng/g(dry mass).The deposition fluxes of NP varied from 0.68 to 17.9 ng/(cm2 · a) with peaks and valleys reflecting the traces of economic development history in China during the previous three decades.BPA was detected at sediment layers deposited from 1973 to 2001 with contents of up to 3.66 ng/g.The fluxes of BPA varied from 0.62 to 3.13 ng/(cm2 · a) showing a similar pattern as NP.The contents of NP and BPA also indicated potential risks on benthic organisms in the study area. | BIAN Haiyan LI Zhengyan LIU Ping PAN Jinfen | 2010 | Acta Oceanologica Sinica2010，29，5： | 10 |
| 6 | Local concentration of middle and heavy rare earth elements in the col on the weathered crust elution-deposited rare earth ores显示文摘This present study focused on investigating the content and relative enrichment multiple of rare earth elements(REEs) in different terrain of weathered ore body, and the value of light rare earth elements/middle and heavy rare earth elements(LREEs/HREEs), in order to understand the mobilization and redistribution of HREEs, Furthermore, the abrasion pH was investigated as well, The results show that the average contents of HREEs in the knap, ridge and col are 37.26, 61.71, and 271.3 μg/g, respectively. The value of LREEs/HREEs is decreased from 16.78 to 7.914 between knap and col and the relative enrichment multiple of HREEs is as follows: C_(Col-HREEs) > C_(Ridge-HREEs) > 0. It is indicated the HREEs is concentrated in the col and the enrichment degree of HREEs is stronger than the ridge and the knap. Rare earth elements fractionation is exhibited in the different terrain of weathered ore body. Based on this study, the finding that HREEs concentrate in the lower terrain of weathered crust elution-deposited rare earth ores, such as in the col, would become a marker or indicator to search for middle and heavy rare earth enriched zone. | Zhenyue Zhang Ningjie Sun Zhengyan He Ru'an Chi | 2018 | Journal of Rare Earths2018，36，5： | 10 |
| 7 | OsSPL18 controls grain weight and grain number in rice显示文摘Grain weight and grain number are two important traits directly determining grain yield in rice. To date,a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines.Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5?RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice. | Hua Yuan Peng Qin Li Hu Shijie Zhan Shifu Wang Peng Gao Jing Li Mengya Jin Zhengyan Xu Qiang Gao Anping Du Bin Tu Weilan Chen Bingtian Ma Yuping Wang Shigui Li | 2019 | Journal of Genetics and Genomics2019，46，1： | 8 |
| 8 | Destruction mechanism of gas explosion to ventilation facilities and automatic recovery technology显示文摘In order to overcome the heavy casualties caused by gas explosion, we verified the propagation law of shock wave in pipeline and the overpressure distribution of gas explosion by similar experiments according to the analyses of reasons for casualty and ventilation system model destroyed by gas explosion in the mining face. We summarized the gas composition after the explosion and its danger, analyzed the effects of the gas explosion shock wave to ventilation system and facilities and the laws of toxic gas spread and diffusion in the ventilation network after the explosion. We presented a technical proposal to control the smoke and recover the ventilation system after a gas explosion and developed a reserve air door and control system that were embed in the lane, and could close automatically in conditions of no pressure and electricity. The results showed that the reserve air door normally opened and could close automatically controlling the smoke flow and resuming the ventilation system when the gas explosion shock wave destroyed the original shutting air door which resulted in the air short circuit. | Wang Kai | 2012 | International Journal of Mining Science and Technology2012，22，3： | 8 |
| 9 | Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.7387显示文摘Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications.To elucidate the regulatory mechanism of neomycin biosynthesis,we completed draft genome sequencing of a neomycin producer Streptomyces fradiae CGMCC 4.7387 from marine sediments,and the neomycin biosynthesis gene cluster was identified.Inactivation of the afsA-g gene encoding a γ-butyrolactone(GBL) synthase in S.fradiae CGMCC 4.7387 resulted in a significant decrease of neomycin production.Quantitative RT-PCR analysis revealed that the transcriptional level of neoR and the aphA-neoGH operon were reduced in the afsA-g::aac(3)Ⅳ mutant.Interestingly,a conserved binding site of AdpA,a key activator in the GBL regulatory cascade,was discovered upstream of neoR,a putative regulatory gene encoding a protein with an ATPase domain and a tetratricopeptide repeat domain.When neoR was inactivated,the neomycin production was reduced about 40%in comparison with the WT strain.Quantitative RT-PCR analysis revealed that the transcriptional levels of genes in the aphA-neoGH operon were reduced clearly in the neoR::aac(3)Ⅳ mutant.Finally,the titers of neomycin were improved considerably by overexpression of qfsA-gand neoR in S.fradiae CGMCC 4.7387. | Xiangxi Meng Wenzhao Wang Zhoujie Xie Pengwei Li Yue Li Zhengyan Guo Yingjian Lu Jie Yang Kaile Guan Zhaoxin Lu Huarong Tan Yihua Chen | 2017 | Science China(Life Sciences)2017，60，9： | 7 |
| 10 | Analysis of vacuum chamber suppressing gas explosion显示文摘In order to suppress the harm of gas explosion,the current study researched on the body of vacuum chamber.The previous studies verifed that it could obviously lower the explosion overpressure by reasonably arranging vacuum chamber on pipe.That is to say,the vacuum chamber has the effect of absorbing wave and energy.To further deeply analyze the vacuum chamber suppressing gas explosion,this research designed the L-type pipe of gas explosion,and compared the experimental results of gas explosion with vacuum chamber and without vacuum chamber.Besides,using the gas chromatograph,this study also investigated the gas compositions in the pipe before and after explosion.The results show that:(1)without vacuum chamber,the maximum value of explosion overpressure is 0.22 MPa,with60 ms duration,and after explosion,the concentration of oxygen drops to 12.07%,but the concentration of carbon monoxide increases to 4392.3 10à6,and the concentration of carbon dioxide goes up to7.848%,which can make the persons in danger suffocate and die;(2)with vacuum chamber,explosion overpressure drops to 0.18 MPa,with 20 ms duration or less,and after explosion,the concentration of oxygen still remains 12.07%,but the concentration of methane is 7.83%,however the concentration of carbon monoxide is only 727.24 10à6,and the concentration of carbon dioxide is only 1.219%,at the this moment the concentration ratio of toxic gas drops by more than 83%in comparison to be that without vacuum chamber.Consequently,the vacuum chamber can guarantee that most methane does not take part in chemical reaction,and timely quenches the deflagration reaction of gas and oxygen.Because of the two points mentioned above,it reduces the explosion energy,and lowers that the overpressure of blast wave impacts and damages on the persons and facilities,and also decreases the consumption of oxygen and the production of the toxic gas.Therefore,it is safe to conclude that the vacuum chamber not only absorbs wave and energy,but also prevents and suppresses explosion. | Shao Hao Jiang Shuguang Li Qinhua Wu Zhengyan | 2013 | International Journal of Mining Science and Technology2013，23，5： | 5 |
| 11 | Application study on complex wetting agent for dust-proof after gas drainage by outburst seams in coal mines显示文摘After gas drainage,the outburst seam is dry and,consequently,during subsequent mechanized mining,unwanted dust production is extensive.Because the hard dustfall typically has a small particle size it contains a high percentage of respirable dust.Considering these factors,this paper improved the efficiency of dustfall by reducing the surface tension of the liquid used as a wetting agent,then configuring five kinds of wetting agents by adopting various doses and types of surfactants,neutral inorganic salts and chemical additives,etc.After purchasing DA-85 and SRJ-1 wetting agent,the surface tension and critical micelle concentration of seven kinds of wetting agents were measured using the capillary rise method.Wetting agent A was confirmed to have the best comprehensive effect.The experiments were optimized by measuring and comparatively analyzing contact angles of pure water,SRJ-1 wetting agent and wetting agent A on the surface of different coal samples using a contact angle measurement instrument(HARKESPCA).By comparative analysis,dust-proof effects of seam,dynamic pressure water infusion and highpressure spray in the tunneling face at the Daning coal mine using pure water,wetting agent SRJ-1and A,it was determined that after using wetting agent A,the average dust concentration in the place of the mining machine driver,10 m and 110 m away from the tunneling front is approximately 300 mg/m^3,240 mg/m^3 and 100 mg/m^3,respectively.This practice increased the dust-proof efficiency dramatically and achieved a good comprehensive dust-proof effect. | Wang Kai Ma Xiaoping Jiang Shuguang Wu Zhengyan Shao Hao Pei Xiaodong | 2016 | International Journal of Mining Science and Technology2016，26，4： | 5 |
| 12 | An experimental study of the effect of ionic liquids on the low temperature oxidation of coal显示文摘Fourier transform infrared spectroscopy(FTIR) and constant heating rate experiments were performed to study the low temperature oxidation of coal treated by an ionic liquid,1-allyl-3-methylimidazolium chloride.The inerting effect of the ionic liquid toward the low temperature oxidation process is discussed.The results show that:(1) The hydroxyl content associated with hydrogen bonds,the aliphatic methyl content,the methylene group content,and the ether oxygen bond content are reduced in the treated coal.At the same time the content of aromatic C@C bonds is constant but these chemical bonds weaken and some substituted aromatic hydrocarbon content increases while other types decrease.This demonstrates that(AMIm)Cl dissolves and destroys the coal surface microstructure;(2) The oxygen consumption of the treated coal is less than what is seen in raw coal.The CO,CO 2,C 2 H 4,and C 2 H 6 content from the treated coal is reduced compared to the untreated coal;(3) The apparent activation energy for the oxidizing reaction is different in the treated and raw coals.Micro-structural changes and macroscopic gas production allow us to conclude that(AMIm)Cl can effectively inhibit low temperature oxidation of coal. | Zhang Weiqing | 2012 | International Journal of Mining Science and Technology2012，22，5： | 5 |
| 13 | Comprehensive Treatment and Prognostic Factors in Patients with Carcinoma of the Cervical Stump显示文摘OBJECTIVE To evaluate the applicability of combined therapyand the prognostic factors in patients with carcinoma of thecervical stump (CCS).METHODS The clinical records of 60 CCS patients whounderwent combined treatment in our hospital during a periodfrom January 2000 to December 2007, were collected andretrospectively analyzed. The prognostic factors were studiedusing univariate analysis. Analytical evaluation of the independentprognostic factors was performed using COX proportionalhazardsregression model.RESULTS The 1-, 3- and 5-year survival rates of the 60 patientswere 95%, 78% and 68%, respectively, with a median survivaltime of 32 months. Univariate survival analysis showed thatthese independent prognostic factors included positive pelviclymph nodes (P = 0.001), lymphovascular tumor embolus (P =0.001), and adjuvant chemotherapy (P = 0.011). In the 60 cases,postoperative local recurrence in the pelvic cavity occurred in1 and distant metastasis in 3. Related complications, such asradiocystitis, recto-vaginal fistula and vesico-vaginal fistula werefound in 6 of the total cases (10%). The serum levels of squamousepithelium antigen detected before and after treatment weresignificantly different (P = 0.000). The incidence of CCS is low;however, the disease is difficult to cure due to the high incidenceof complications and to the frequency of distant metastasis.Therefore, individualized treatment is needed. Complications fromsubtotal hysterectomy (STH) should be treated and controlledaggressively. Careful follow-up as well as close monitoring andobservation for significant symptoms in the postoperative coursewill enhance clinical outcome.CONCLUSION Cancer of the cervical stump has a lowmorbidity and severe complications, and most recurrences aredistant metastases. Because it is difficult to cure, there is a needto design a treatment regimen for each individual patient basedon the factors deemed as high risk. The surgical indications forsubtotal uterine resection should be followed and close follow-upafter surgery should be maintained. | Lu Chen Ting Xia Zhengyan Yang Jianqing Zhu | 2009 | Clinical oncology and cancer resexreh2009，6，6： | 4 |
| 14 | Identifiability and identification of a Synthesis Load Model显示文摘A Synthesis Load Model (SLM) including both the power load and the distribution network has been proposed in the references. The identifiability of SLM is analyzed at first, it is concluded that the model parameters are identifiable if one of the resistance, reactance and the ratio of them is known. The conclusion is validated through a simulation example. A strategy for parameter identification of SLM is proposed with the combination of the component based approach and the measurement based approach. During parameter identification, only the key parameters playing very important roles in the dynamics of the load and the system are estimated, while the other parameters playing limited role are set as the default values. The proposed strategy is verified by the field measurements. | JU Ping, JIN YuQing, CHEN Qian, SHAO ZhengYan & MAO Chao College of Electrical Engineering, Hohai University, Nanjing 210098, China | 2010 | Science China(Technological Sciences)2010，53，2： | 4 |
| 15 | Distributions of Inorganic Nutrients in the Bohai Sea of China显示文摘To study the contents and distribution of inorganic nutrients in the Bohai Sea of China, two cruise surveys were undertaken in August (summer) 2000 and January (winter) 2001, respectively. A total of 595 water samples were collected from 91 stations and five nutrients, i.e., nitrate, nitrite, ammonia, phosphate and silicate, were analyzed for each sample. The results show that the average concentration of dissolved inorganic nitrogen (DIN) in the Bohai Sea in winter (6.529 μmol L -1) is significantly higher than that in summer (3.717 μmol L -1). The phosphorus concentration in winter (0.660 μmol L -1) is also significantly higher than that in summer (0.329 μmol L -1). Mean silicate concentration in winter (7.858 μmol L -1) is, however, not significantly different from that in summer (7.200 μmol L -1). Nutrients also vary considerably in different areas in Bohai Sea. DIN concentration in the Laizhou Bay (4.444 μmol L -1), for example, is significantly higher than those in the Bohai Bay (2.270 μmol L -1) and Bohai Strait (2.431 μmol L -1), which is caused by the discharge of large amounts of nitrogen into Laizhou Bay via Yellow River. The nutrients show different vertical distribution patterns. In summer, nutrients in bottom layer are generally richer than those in surface and middle layers. In winter, however, nutrients are not significantly different in different layers. Compared with historic data, DIN contents have increased continually since the early 1980 s. Based on atomic ratios of different nutrients, nitrogen is still the limiting factor for algal growth in the Bohai Sea. | LI Zhengyan *, BAI Jie, SHI Jinhui, GAO Huiwang College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, P..R.China | 2003 | Journal of Ocean University of Qingdao2003，2，1： | 4 |
| 16 | Dopaminergic effects on in vitro osteogenesis显示文摘Multiple growth factors(e.g., BMP2, TGF-b1, FGF2) and isolated genes have been shown to improve osteoblastic proliferation and mineralization, advancing bone tissue engineering. Among these factors, both polydopamine(PDA) and dopamine(DA) monomer have recently been reported to increase osteoblast proliferation and mineralization in vitro. Although a well-characterized neurotransmitter, DA's role in the bone is unknown. We hypothesize that DA can directly act on osteoblasts, and examined whether osteoblasts express DA receptors that respond to exogenous DA. m RNAs and protein cell lysates were obtained from MC3T3-E1 cells during osteogenic differentiation phase. Reverse transcription polymerase chain reaction and western blot analysis were used to examine the expression of DA receptors, D1–D5. Dose-response effect and time course of DA treatment on cell proliferation, mineralization, and osteogenic differentiation were investigated at pre-determined days. Real-time PCR was performed to investigate whether DA affects osteogenic gene expression(ALP, BSP, OC, OSX, RUNX2, and Collagen1a2) with or without receptor antagonists(SCH233390 and GR103691). Two-way ANOVA was used for statistical analysis. All five DA receptors(D1, D2, D3, D4, and D5) m RNAs and proteins were expressed in MC3T3-E1 cells. DA treatment increased cell proliferation for up to 7 days(P, 0.05). Osteogenic mineralization was significantly greater in the DA-treated group than control group(P, 0.05). Finally, expression of all the osteogenic genes was inhibited by DA receptor antagonists for D1, D3, and D5. Our findings suggest that MC3T3-E1 osteoblasts express functional DA receptors that enhance proliferation and mineralization. PDA is not biologically inert and has important implications in orthopedic applications. Furthermore, osteoblast differentiation might be regulated by the nervous system, presumably during bone development, remodeling, or repair. | Dong Joon Lee Henry C Tseng Sing Wai Wong Zhengyan Wang Meng Deng Ching-Chang Ko | 2015 | Bone Research2015，3，3： | 3 |
| 17 | Empirical likelihood inference for diffusion processes with jumps显示文摘In this paper, we consider the empirical likelihood inference for the jump-diffusion model. We construct the confidence intervals based on the empirical likelihood for the infinitesimal moments in the jump-diffusion models. They are better than the confidence intervals which are based on the asymptotic normality of point estimates. | Lin ZhengYan Wang HanChao | 2010 | Science China Mathematics2010，53，7： | 3 |
| 18 | Serological characterization of Haemophilus parasuis isolates from China显示文摘 | Xuwang Cai Huanchun Chen P.J. Blackall Zhengyan Yin Lei Wang Zhengfei Liu Meilin Jin | 2005 | Veterinary Microbiology2005，，3： | 3 |
| 19 | Consistency of kernel density estimators for causal processes显示文摘Using the blocking techniques and m-dependent methods,the asymptotic behavior of kernel density estimators for a class of stationary processes,which includes some nonlinear time series models,is investigated.First,the pointwise and uniformly weak convergence rates of the deviation of kernel density estimator with respect to its mean(and the true density function)are derived.Secondly,the corresponding strong convergence rates are investigated.It is showed,under mild conditions on the kernel functions and bandwidths,that the optimal rates for the i.i.d.density models are also optimal for these processes. | LIN ZhengYan ZHAO YueXu | 2014 | Science China Mathematics2014，57，5： | 3 |
| 20 | Reweighted Nadaraya-Watson estimation of jump-diffusion models显示文摘In this paper,we study the nonparametric estimation of the second infinitesimal moment by using the reweighted Nadaraya-Watson (RNW) approach of the underlying jump diffusion model.We establish strong consistency and asymptotic normality for the estimate of the second infinitesimal moment of continuous time models using the reweighted Nadaraya-Watson estimator to the true function. | HANIF Muhammad WANG HanChao LIN ZhengYan | 2012 | Science China Mathematics2012，55，5： | 3 |
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