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| 1 | Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs显示文摘A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying the development,homeostasis,and disease of human intervertebral disks(IVDs)remains challenging.Here,the transcriptomic landscape of 108108 IVD cells was mapped using single-cell RNA sequencing of three main compartments from young and adult healthy IVDs,including the nucleus pulposus(NP),annulus fibrosus,and cartilage endplate(CEP).The chondrocyte subclusters were classified based on their potential regulatory,homeostatic,and effector functions in extracellular matrix(ECM)homeostasis.Notably,in the NP,a PROCR+resident progenitor population showed enriched colony-forming unit-fibroblast(CFU-F)activity and trilineage differentiation capacity.Finally,intercellular crosstalk based on signaling network analysis uncovered that the PDGF and TGF-βcascades are important cues in the NP microenvironment.In conclusion,a single-cell transcriptomic atlas that resolves spatially regulated cellular heterogeneity together with the critical signaling that underlies homeostasis will help to establish new therapeutic strategies for IVD degeneration in the clinic. | Yibo Gan Jian He Jun Zhu Zhengyang Xu Zhong Wang Jing Yan Ou Hu Zhijie Bai Lin Chen Yangli Xie Min Jin Shuo Huang Bing Liu Peng Liu | 2021 | Bone Research2021，9，3： | 3 |
| 2 | Molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme(CYP11A1) gene during the reproductive cycle in goose(Anas cygnoides)显示文摘Background: CYP11A1, a gene belonging to the family 11 of cytochrome P450, encodes a crucial steroidogenic enzyme that catalyzes the initial step in the production of all classes of steroids. Many studies show that CYP11A1 plays a role in ovary function. However, the role of CYP11A1 in goose reproductive cycle remains largely unknown.Results: In this study, full-length CYP11A1 c DNA of Zhedong goose was obtained using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of c DNA ends(RACE). The c DNA consisted of a 96-base pair(bp) 5′untranslated region(UTR), a 179-bp 3′UTR and a 1509-bp open reading frame. The open reading frame encodes a putative 503 amino acid protein that shares high homology with CYP11A1 of other birds. The amino acid sequence possesses conserved domains of the P450 superfamily, which include the steroid-binding domain and the heme-binding region. Real-time quantitative polymerase chain reaction(q PCR) analysis revealed CYP11A1 mR NA was expressed ubiquitously in every Zhedong goose tissue analyzed, including the heart, liver, glandular stomach,lung, spleen, kidney, intestinum tenue, intestinum crassum, cerebrum, cerebellum, muscle, oviduct, pituitary,hypothalamus and ovary.. The relatively low levels of CYP11A1 m RNA were detected in pituitary, ovary and oviduct tissues at ovulation when compared with levels at oviposition. Interestingly, higher expression was observed in ovary and oviduct tissues during brooding. Lastly, higher m RNA expression of Yangzhou geese was detected during the ovulation period than that of Zhedong geese.Conclusions: Our findings reveal the sequence characterization and expression patterns of the CYP11A1 gene during the goose reproductive cycle, which may provides correlative evidence that CYP11A1 expression is important in reproduction activity. | Qi Xu Yadong Song Yang Chen Ran Liu Yang Zhang Yang Li Zhengyang Huang Wenming Zhao Guobin Chang Guohong Chen | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
| 3 | Differential proteome profiling of pleural effusions from lung cancer and benign inflammatory disease patients显示文摘 | Zhengyang Wang Cheng Wang Xiaobin Huang Ying Shen Jing Shen Kejing Ying | 2012 | BBA - Proteins and Proteomics2012，，4： | 1 |
| 4 | Temperature-driven reversible structural transformation and conductivity switching in ultrathin Cu_(9)S_(5)crystals显示文摘Two-dimensional(2D)materials with reversible phase transformation are appealing for their rich physics and potential applications in information storage.However,up to now,reversible phase transitions in 2D materials that can be driven by facile nondestructive methods,such as temperature,are still rare.Here,we introduce ultrathin Cu_(9)S_(5)crystals grown by chemical vapor deposition(CVD)as an exemplary case.For the first time,their basic electrical properties were investigated based on Hall measurements,showing a record high hole carrier density of~1022 cm^(-3) among 2D semiconductors.Besides,an unusual and repeatable conductivity switching behavior at~250 K were readily observed in a wide thickness range of CVD-grown Cu_(9)S_(5)(down to 2 unit-cells).Confirmed by in-situ selected area electron diffraction,this unusual behavior can be ascribed to the reversible structural phase transition between the room-temperature hexagonalβphase and low-temperatureβ’phase with a superstructure.Our work provides new insights to understand the physical properties of ultrathin Cu_(9)S_(5)crystals,and brings new blood to the 2D materials family with reversible phase transitions. | Lei Zhang Zeya Li Ying Deng Li Li Zhansheng Gao Jiabiao Chen Zhengyang Zhou Junwei Huang Weigao Xu Xuewen Fu Hongtao Yuan Feng Luo Jinxiong Wu | 2023 | Nano Research2023，16，7： | 0 |
| 5 | The first chromosome-level Fallopia multiflora genome assembly provides insights into stilbene biosynthesis显示文摘Fallopia multiflora(Thunb.)Harald,a vine belonging to the Polygonaceae family,is used in traditional medicine.The stilbenes contained in it have significant pharmacological activities in anti-oxidation and anti-aging.This study describes the assembly of the F.multiflora genome and presents its chromosome-level genome sequence containing 1.46 gigabases of data(with a contig N50 of 1.97 megabases),1.44 gigabases of which was assigned to 11 pseudochromosomes.Comparative genomics confirmed that F.multiflora shared a whole-genome duplication event with Tartary buckwheat and then underwent different transposon evolution after separation.Combining genomics,transcriptomics,and metabolomics data to map a network of associated genes and metabolites,we identified two FmRS genes responsible for the catalysis of one molecule of p-coumaroyl-CoA and three molecules of malonyl-CoA to resveratrol in F.multiflora.These findings not only serve as the basis for revealing the stilbene biosynthetic pathway but will also contribute to the development of tools for increasing the production of bioactive stilbenes through molecular breeding in plants or metabolic engineering in microbes.Moreover,the reference genome of F.multiflora is a useful addition to the genomes of the Polygonaceae family. | Yujiao Zhao Zhengyang Yang Zhongren Zhang Minzhen Yin Shanshan Chu Zhenzhen Tong Yuejian Qin Liangping Zha Qingying Fang Yuan Yuan Luqi Huang Huasheng Peng | 2023 | Horticulture Research2023，10，5： | 0 |
| 6 | Identification and Functional Analysis of Serum Specific miRNAs in Recurrent Aphthous Stomatitis Patients with Excess-heat or Yin-deficiency显示文摘Background:The treatment of Traditional Chinese Medicine(TCM)in recurrent aphthous stomatitis(RAS)based on syndrome differentiation has won the international acceptance,but the molecular mechanism of excess-heat syndrome and yin-deficiency syndrome of RAS remains unclear.Objective:To clarify specific microRNAs(miRNAs)and their functions in RAS patients with excess-heat or yin-deficiency.Methods:Serum samples were collected from patients meeting the RAS diagnostic criteria of excess-heat or yin-deficiency syndrome and healthy individuals.Core miRNAs were then identified under miRNA microarray anal-yses.Target prediction and bioinformatic analyses were carried out and gene-pathway-networks were visualized to better understand the relationship between different genes and pathways.Results:(1)90 individuals meeting the inclusion criteria were collected in this study.Among them,9 miRNAs were screened out in excess-heat syndrome group(EH group),with 1 upregulated and 8 downregulated.And four miRNAs(hsa-miR-20b-5p,hsa-miR-122-5p,hsa-miR-483-5p and hsa-miR-3197)were validated by real-time PCR method.14 miRNAs were screened out in yin-deficiency syndrome group(YD group)(7 upregulated and 7 downregulated).And hsa-miR-17-5p,hsa-miR-106-5p and hsa-miR-20b-5p were validated.(2)A total of 4,776 target genes were identified in EH group which enriched in GO categories including nervous system development and calcium ion binding and pathway such as PI3K-Akt signaling pathway and Calcium signaling pathway.10,172 target genes were identified in YD group which enriched GO categories included protein binding and positive regulation of transcription from RNA polymerase II promoter and pathway included MAPK signaling pathway and Ras signaling pathway.Conclusion:Hsa-miR-20b-5p in patients with RAS could act as the novel target for the classification of the syndrome.It is upregulated in RAS patients with excess-heat syndrome while downregulated in patients with yin-deficiency syndrome.The PI3K-Akt and MAPK signaling pathways and their related target genes may provide new insights into the molecular mechanisms of RAS with excess-heat syndrome or yin-deficiency syndrome,respectively. | Jie Bao Zhengyang Zhu Xizhao Zhang Lin Huang Li Xu Xiaobing Dou Yongsheng Fan | 2023 | Clinical Complementary Medicine and Pharmacology2023，3，4： | 0 |
| 7 | Synergy to discovery and innovation——Growth of nanorods显示文摘A synergy of computer simulations, analytical formulations, and experiments proves very effective in research and development. Using nanorod growth as an example, this letter presents the synergy of the three complementary approaches in research, and demonstrates its effectiveness. Through this synergy, a theoretical framework of nanorod growth emerges; through the direction of the theories,experimental realization of smallest well-separated nanorods becomes reality; through the realization of such nanorods, metallic glue in ambient environments becomes technical reality and commercial possibility. | Zhengyang Li Hanchen Huang | 2016 | Theoretical & Applied Mechanics Letters2016，6，6： | 0 |
| 8 | Learning physical characteristics like animals for legged robots显示文摘Physical characteristics of terrains,such as softness and friction,provide essential information for legged robots to avoid non-geometric obstacles,like mires and slippery stones,in the wild.The perception of such characteristics often relies on tactile perception and vision prediction.Although tactile perception is more accurate,it is limited to close-range us e;by contrast,establishing a supervised or self-supervised contactless prediction system using computer vision re quires adequate labeled data and lacks the ability to adapt to the dynamic environment.In this paper,we simulate the behavior of animals and propose an unsupervised learning framework for legged robots to learn the physical characteristics of terrains,which is the first report to manage it online,incrementally and with the ability to solve cognitive conflicts.The proposed scheme allows robots to interact with the environment and adjust their cognition in real time,therefore endowing robots with the adaptation ability.Indoor and outdoor experiments on a hexapod robot are carried out to show that the robot can extract tactile and visual features of terrains to create cognitive networks independently;an associative layer between visual and tactile features is created during the robot’s exploration;with the layer,the robot can autonomously generate a physical segmentation model of terrains and solve cognitive conflicts in an ever-changing environment,facilitating its safe navigation. | Peng Xu Liang Ding Zhengyang Li Huaiguang Yang Zhikai Wang Haibo Gao Ruyi Zhou Yang Su Zongquan Deng Yanlong Huang | 2023 | National Science Review2023，10，5： | 0 |
| 9 | Heptagonal intramolecular-lock strategy enables high-performance thermally activated delayed fluorescence emitters显示文摘The development of highly efficient thermally activated delayed fluorescence(TADF)emitters is persistently pursued for the application of organic light-emitting diodes(OLED)in full-colour display and solid-state lighting.Herein,we present a heptagonal intramolecular-lock strategy to design high-performance TADF emitters.As a proof-of-concept,a new type of tribenzotropone(TBP)acceptor has been designed and synthesized by a cascade decarboxylative cyclization of aryl oxoacetic acid derivative with biphenyl boronic acid.Compared with the unlocked benzophenone(BP)acceptor,the TBP acceptor has a highly twisted heptagonal geometry with moderate rigidity and flexibility,which enables a high-performance TADF emitter with a small single-triplet energy gap(ΔE_(ST))of 0.04 e V,a high photoluminescence quantum yield(Φ_(PL))of 99% and a large horizontal orientation factor(Θ_(//))of 84.0%.Consequently,highly efficient OLEDs with an external quantum efficiency as high as 33.8% are assembled,which is significantly higher than those of DPAC-BP with a highly rotatable BP acceptor(23.8%)as well as DPACFO with a rigid fluorenone(FO)acceptor(6.9%). | Zhenmei Huang Dezhi Yang Dongge Ma Zhengyang Bin Jingsong You | 2024 | Science China Chemistry2024，67，4： | 0 |
| 10 | Genetic interrogation of phenotypic plasticity informs genome-enabled breeding in cotton显示文摘Phenotypic plasticity, or the ability to adapt to and thrive in changing climates and variable environments, is essential for developmental programs in plants. Despite its importance, the genetic underpinnings of phenotypic plasticity for key agronomic traits remain poorly understood in many crops. In this study, we aim to fill this gap by using genome-wide association studies to identify genetic variations associated with phenotypic plasticity in upland cotton (Gossypium hirsutum L.). We identified 73 additive quantitative trait loci (QTLs), 32 dominant QTLs, and 6799 epistatic QTLs associated with 20 traits. We also identified 117 additive QTLs, 28 dominant QTLs, and 4691 epistatic QTLs associated with phenotypic plasticity in 19 traits. Our findings reveal new genetic factors, including additive, dominant, and epistatic QTLs, that are linked to phenotypic plasticity and agronomic traits. Meanwhile, we find that the genetic factors controlling the mean phenotype and phenotypic plasticity are largely independent in upland cotton, indicating the potential for simultaneous improvement. Additionally, we envision a genomic design strategy by utilizing the identified QTLs to facilitate cotton breeding. Taken together, our study provides new insights into the genetic basis of phenotypic plasticity in cotton, which should be valuable for future breeding. | Yuefan Huang Zhengyang Qi Jianying Li Jiaqi You Xianlong Zhang Maojun Wang | 2023 | Journal of Genetics and Genomics2023，50，12： | 0 |
| 11 | Brexit and Its Impact on the US Stock Market显示文摘This paper firstly analyzes the Brexit’s impact on the US stock market using a novel interval methodology. The interval-valued dummy variables are proposed to measure the direction and magnitudes of the changes in the inter-day trend and the intra-day volatility of S&P500 returns simultaneously. It is found that both the trend and the volatility of S&P500 returns increased before the Brexit. Besides, the Brexit negatively affected S&P500 returns’ trend in the short term after the event,while its impact on market volatility was positive, which slowly decayed across time. Furthermore, a new interesting finding is that there are both short-term momentum effects(i.e., positive autocorrelation of trends) and volatility clustering in stock markets. | QIAO Kenan LIU Zhengyang HUANG Bai SUN Yuying WANG Shouyang | 2021 | Journal of Systems Science & Complexity2021，34，3： | 0 |
| 12 | Lck/Hck/Fgr介导的TBK1酪氨酸磷酸化负调TBK1功能及抗病毒天然免疫应答显示文摘抗病毒天然免疫通路的激活,传统上认识是由蛋白泛素化和蛋白丝苏氨酸磷酸化所介导,对于蛋白酪氨酸磷酸化修饰的作用与生理功能,未有深入探索和认知。课题组发现病毒感染过程中,在多种器官来源的细胞中都观察到细胞内源TBK1有动态的酪氨酸磷酸化修饰,并与TBK1激活与抑制紧密相关。 | Shengduo Liu Shasha Chen Xinran Li Shiying Wu Qian Zhang Qiuheng Jin Lin Hu Ruyuan Zhou Zhengyang Yu Fansen Meng Siwen Wang Yaowei Huang Sheng Ye Li Shen Zongping Xia Jian Zou Xin-Hua Feng 徐平龙 | 2018 | 科学新闻2018，0，4： | 0 |
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