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| 1 | Discovery of Metastasis-Associated Biomarkers in Ovarian Cancer Using SELDI-TOF: An in Vitro and Clinical Study显示文摘OBJECTIVE To identify metastasis-related biomarkers in humanovarian cancer cell lines and in serum.METHODS We isolated total protein from cell lysis solutionsand cultured supernatants from 2 human ovarian cancer cell linesand used SELDI-TOF-MS to detect the differential expressionof the proteins in the 2 cell lines.The proteomic spectra weregenerated using weak cation exchange chips.The biomarkerswere validated by analyzing serum proteins or peptides in ovariancancer patients,relapsed ovarian cancer patients,patients withbenign ovarian tumors,and healthy people.RESULTS Four proteins in the culture supernatant fromHO-8910PM cells were up-regulated,relative to the culturesupernatant of HO-8910 cells.One protein (3,144 Da m/z value)was up-regulated in both the cell lysis solution and in the culturesupernatant of HO-8910PM cells.In addition,expression of the3,144 Da m/z protein differed significantly between serum fromthe 26 ovarian cancer patients,from the 22 relapsed ovarianpatients and from the 37 healthy women (P<0.01).However,therewas no difference between patients with benign ovarian tumorsand healthy people (P>0.5).CONCLUSION Ovarian cancer cell lines with high or lowmetastatic potential have distinct protein profiles.Protein 3,144Da m/z could be a useful biomarker for diagnosing ovarian cancermetastasis. | Zhiguo Zheng Yun Gao Linhui Gu Hanzhou Mou Chihong Zhu Jianqing Zhu Shenhua Xu | 2009 | Clinical oncology and cancer resexreh2009，6，5： | 6 |
| 2 | Dye-sensitized solar cells based on anatase TiO 2 /multi-walled carbon nanotubes composite nanofibers photoanode显示文摘 | Pingfan Du Lixin Song Jie Xiong Ni Li Lijun Wang Zhenqiang Xi Naiyan Wang Linhui Gao Hongliang Zhu | 2013 | Electrochimica Acta2013，，： | 1 |
| 3 | Hydrothermal synthesis of blue-emitting YPO4:Yb3+ nanophosphor显示文摘 | Guangfa WANG Linhui GAO Hongliang ZHU Weijie ZHOU | 2016 | Frontiers of Materials Science2016，10，2： | 1 |
| 4 | Removal of methyl orange from aqueous solutions with poly(acrylic acid co acryl- amide) superabsorbent resin 显示文摘 | Tang Yaoji Wang Xing Zhu Linhui | 2013 | Polymer Bulletin2013，70，90： | 1 |
| 5 | KLF4 facilitates chromatin accessibility remodeling in porcine early embryos显示文摘Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development. | Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu Yi-Liang Miao | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 6 | Initial experience of laparoendoscopic single-site radical prostatectomy with a novel purpose-built robotic system显示文摘Objective This prospective single-arm clinical trial aimed to evaluated the feasibility and safety of the application of the SHURUI system(Beijing Surgerii Technology Co.,Ltd.,Beijing,China),a novel purpose-built robotic system,in single-port robotic radical prostatectomy.Methods Sixteen patients diagnosed with prostate cancer were prospectively enrolled in and underwent robotic radical prostatectomy from October 2021 to August 2022 by the SHURUI single-port robotic surgical system.The demographic and baseline data,surgical,oncological,and functional outcomes as well as follow-up data were recorded.Results The mean operative time was 226.3(standard deviation[SD]52.0)min,and the mean console time was 183.4(SD 48.3)min,with the mean estimated blood loss of 116.3(SD 90.0)mL.The mean length of postoperative hospital stay was 4.50(SD 0.97)days.Two patients had postoperative complications(Clavien-Dindo Grade II),and both patients improved after conservative treatment.All patients’postoperative prostate-specific antigen levels decreased to below 0.2 ng/mL 1 month after discharge.The mean prostate-specific antigen level further decreased to a mean of 0.0219(SD 0.0641)ng/mL 6 months after surgery.Thirty days postoperatively,12 out of 16 patients reported using no more than one urinary pad per day,and all patients reported satisfactory urinary control without the need for pads 6 months after surgery.Conclusion The SHURUI system is safe and feasible in performing radical prostatectomy via both transperitoneal and extraperitoneal approaches.Tumor control and urinary continence were satisfying for patients enrolled in.The next phase involves conducting a large-scale,multicenter randomized controlled trial to thoroughly assess the effectiveness and safety of the new technology in a broader population. | Zheng Wang Chao Zhang Chengwu Xiao Yang Wang Yu Fang Baohua Zhu Shouyan Tang Xiaofeng Wu Hong Xu Yi Zhou Lingfen Wu Zhenjie Wu Bo Yang Yi He Yi Liu Linhui Wang | 2023 | Asian Journal of Urology2023，10，4： | 0 |
| 7 | Numerical Study and Performance Analyses of Mems-Based Particle Velocity Sensor with Combined Inclined Rib Pair显示文摘The combined inclined rib pair(CIRP)is the first time proposed to improve the sensor performance of particle velocity sensor(PVS)by using a three-dimensional numerical method.The method is verified by the experiment results in the literature.The optimal plain channel parameters are determined as the basic sensor structure.In comparison of plain channel,both heat transfer characteristics and sensor performance are enhanced effectively by arranging the CIRP.The reason is that the high flow rate region caused by the CIRP can maintain strongly in the whole fluid field if there are enough rib pairs.Furthermore,the produced longitudinal vortex pair can get a better fluid mix,which is more conductive to heat transfer.The increasing height and number of the CIRP can improve the heat transfer characteristics,but the flow resistance will increase as well.For the purpose of finding the best overall performance,the effects of the parameters including the geometric sizes and the position of the CIRP have been investigated.The results show that PVS will get the best sensitivity when the rib length and width are 0.2 mm and 0.03 mm respectively,and the distance between rib pair and between ribs in the same pair are 0.15 mm and 0.3 mm respectively.Besides,the most suitable crossing angle is 45°.Thus,the performance of PVS can be significantly improved by this novel structure. | ZHU Linhui ZENG Yibo GUO Hang | 2022 | Journal of Thermal Science2022，31，5： | 0 |
| 8 | Experimental study for frequency spectrum of turbulent boundary layer pressure fluctuation显示文摘An experimental study on TBL (turbulent boundary layer) pressure fluctuation frequeny spectrum of a revolution body is presented. With the measured results, a relation of convective frequency fo of the models is obtained. Relations of turbuleat wall-pressure fluctuation spectrum in transition region and development region to frequency and speed are obtained | PENG Linhui LU Jianhui (Ocean University of Qingdao Qingdao 266003) ZHU Xiqing SUN Yudong (CSSRC Wuxi 214082) | 1999 | Chinese Journal of Acoustics1999，18，3： | 0 |
| 9 | 吉西他滨联合放射对人高转移卵巢癌细胞的体外实验研究(英文)显示文摘Objective: The aim of the study was to investigate the mechanism of gemcitabine (GEM) combination with radiation on the high metastasis human ovarian cancer cell line (HO-8910PM). Methods: Human ovarian cancer cell line HO-8910PM was treated with different concentrations of gemcitabine for 24 h, then the cells were counted. In the study of GEM combination with radiation, an efficiency of colony formation was observed; the cell cycle and apoptosis were analyzed by flow cytometry; the experiment of depend on the time and its radio sensitivity were observed by using mitotic index with the cells for each 24, 48, 72 and 96 h after experiment. Results: It suggested that the GEM had an inhibition effect on the human ovarian cancer cell line. The alive cell numbers were decreased by following a height of GEM concentration. When GEM in combination with a radiation, the suppression was significantly increased than that of single GEM therapy. The efficiency of colony formation was significantly lower, under this condition the cell could be arrested at G0-G1 phase and could be decreased to enter into the S phase; the apoptosis percentage could be significantly increased; especially, under the 4 Gy and 6 Gy doses the cell apoptosis was more obvious. GEM combination with radiation had depended on the time to the cells; mitotic index of the cells in combination group was observed significantly lower than that of single GEM therapy or single radiation, and this showed that it had an effect of radiosensitivity. Conclusion: The GEM has a significant growth inhibition on the human ovarian cancer cells, GEM combination with radiation could induce HO-8910PM cell occurred arrested and apoptosis. It has depended on the time and has a radiosensitivity effect. The result shows that it is a better method to treat the human ovarian cancer by using radiotherapy combined with gemcitabine. | Linhui Gu Lijuan Qian Chihong Zhu Yutian Ling Hongwei Zhang, Xianglin Liu | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，11： | 0 |
| 10 | Truncated N-terminal huntingtin fragment with expanded-polyglutamine (htt552-100Q) suppresses brain-derived neurotrophic factor transcription in astrocytes显示文摘尽管 huntingtin (htt ) 能被 caspases, calpains,和 aspartyl 朊酶在许多地点劈开,氨基酸(aa ) 552 在人的亨廷顿为劈开被定义为一个比较喜欢的地点在 vivo 的疾病(HD ) 大脑。迄今为止,野类型的 N 终端 htt 碎片 1-552 aa (htt552 ) 的正常功能和它变异的 htt552 的病理学的角色仍然是未知的。尽管变异的 htt (mhtt ) 也在星形细胞被表示, mhtt 是否并且怎么在大部分未知的 HD 遗体通过星形细胞贡献 neurodegeneration。在这研究,一个神经胶质 HD 模型,用到快速的野类型的 htt552 (htt552-18Q ) 和它在老鼠的变化(htt552-100Q ) 的 adenoviral 向量主要外皮的星形细胞,被产生在导出大脑的神经营养的因素(BDNF ) 的抄写上调查 htt552 的影响。从酶的结果连接了 immunosorbent 试金证明在调节星形细胞的媒介的 BDNF 的水平在表示 htt552-100Q 的星形细胞被减少。量的即时聚合酶链反应证明 htt552-100Q 减少了 BDNF III 的抄本,因此, IV 镇压了 BDNF 的抄写。而且, immunofluorescence 证明 htt552-100Q 形成的总数骗诱抄写因素营地反应元素绑定蛋白质和 stimulatory 蛋白质 1,它可能说明 BDNF 抄写的减小。这些调查结果建议 mhtt552 在星形细胞减少 BDNF 抄写,它可能在 HD 贡献 neuronal 机能障碍。 | Linhui Wang Fang Lin Jin Wang Junchao Wu Rong Han Lujia Zhu Guoxing Zhang Marian DiFiglia Zhenghong Qin | 2012 | Acta Biochimica et Biophysica Sinica2012，44，3： | 0 |
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