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1篇 您的检索式：作者名="Zule Cheng"

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		题名	作者	年代	出处	被引量
	1	Chip-based visual detection of micro RNA using DNA-functionalized gold nanoparticles显示文摘In the present study, we developed a highly sensitive and convenient biosensor consisting of gold nanoparticle(Au NP) probes and a gene chip to detect micro RNAs(mi RNAs). Specific oligonucleotides were attached to the glass surface as capture probes for the target mi RNAs, which were then detected via hybridization to the Au NP probes. The signal was amplified via the reduction of HAu Cl4 by H2O2. The use of a single Au NP probe detected 10 pmol L?1 of target mi RNA. The recovery rate for mi R-126 from fetal bovine serum was 81.5%–109.1%. The biosensor detection of mi R-126 in total RNA extracted from lung cancer tissues was consistent with the quantitative PCR(q PCR) results. The use of two Au NP probes further improved the detection sensitivity such that even 1 fmol L?1 of target mi R-125a-5p was detectable. This assay takes less than 1 h to complete and the results can be observed by the naked eye. The platform simultaneously detected lung cancer related mi R-126 and mi R-125a-5p. Therefore, this low cost, rapid, and convenient technology could be used for ultrasensitive and robust visual mi RNA detection.	Ping Wang Jianlong Zhao Bin Hu Zule Cheng Yanan Bai Qinghui Jin Huiying Liu Hongju Mao Sanqiang Li Jianlong Zhao	2016	Science China(Life Sciences)2016，59，5：	0

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