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| 1 | PTEN编码产物在胃癌发生发展不同阶段中的表达及意义显示文摘目的观察抑癌基因PTEN编码蛋白在癌旁胃黏膜、肠上皮化生、异型增生及胃癌组织中的表达,探讨PTEN表达在胃癌发生发展过程中的作用。方法 采用SABC免疫组化方法检测了184例胃癌及癌旁胃黏膜、肠上皮化生和异型增生中的PTEN蛋白表达,比较其与胃癌的临床病理分期、淋巴结转移、Lauren分型及组织学分型的关系。并检测了其中60例胃癌中血管内皮生长因子(VEGF)的蛋白表达,比较VEGF与PTEN蛋白表达的关系。结果PTEN蛋白在癌旁胃黏膜、肠化生、异型增生和胃癌中的阳性表达率分别为100.0%(102/102)、98.5%(65/66)、66.7%(4/6)和47.8%(88/184),后两者的阳性率均显著低于前两者(P<0.01);进展期胃癌PTEN表达显著低于早期胃癌(42.9%:67.6%,P<0.01);PTEN蛋白表达降低与胃癌淋巴结转移呈显著正相关(40.3%:63.3%,P<0.01);弥漫型胃癌PTEN表达显著低于肠型胃癌(41.5%:57.80%,P<0.05);印戒细胞癌中PTEN表达最低(25.0%,7/28),显著低于高中分化管状腺癌(P<0.01)。PTEN蛋白与VEGF蛋白表达呈负相关趋势,但差异无显著性(P>0.05)。结论PTEN基因编码蛋白在胃癌发生发展不同阶段表达呈进行性下调或缺失,可能系通过降低细胞黏附、促进血管形成和提高细胞运动性等途径参与胃癌的发生和演进过程。 | 郑华川 陈颖 况立革 杨琳 李锦毅 吴东瑛 张素敏 辛彦 | 2003 | 中华肿瘤杂志2003，25，1： | 68 |
| 2 | Expression of estrogen receptor and estrogen receptor messenger RNA in gastric carcinoma tissues显示文摘AIM: To study estrogen receptor (ER) and estrogen receptor messenger RNA (ERmRNA) expression in gastric carcinoma tissues and to investigate their association with the pathologic types of gastric carcinoma.METHODS: The expression of ER and ERmRNA in gastric carcinoma tissues (15 males and 15 females, 42-70 years old) was detected by immunohistochemistry and in situ hybridization, respectively.RESULTS: The positive rate of ER (immunohistochemistry)was 33.3% in males and 46.7% in females. In Borrmann Ⅳ gastric carcinoma ER positive rate was greater than that in other pathologic types, and in poorly differentiated adenocarcinoma and signet ring cell carcinoma the positive rates were greater than those in other histological types of both males and females (P<0.05). The ER was more highly expressed in diffused gastric carcinoma than in non-diffused gastric carcinoma (P<0.05). The ER positive rate was also related to regional lymph nodes metastases (P<0.05), and was significantly higher in females above 55 years old, and higher in males under 55 years old (P<0.05). The ERmRNA (in situ hybridization) positive rate was 73.3% in males and 86.7% in females. The ERmRNA positive rates were almost the same in Borrmann Ⅰ, Ⅱ, Ⅲ and Ⅳ gastric carcinoma (P>0.05). ERmRNA was expressed in all tubular adenocarcinoma, poorly differentiated adenocarcinoma and signet ring cell carcinoma (P<0.05). The ERmRNA positive rate was related to both regional lymph nodes metastases and gastric carcinoma growth patterns, and was higher in both sexes above 55 years old but without statistical significance (P>0.05). The positive rate of ERmRNA expression by in situ hybridization was higher than that of ER expression by immunohistochemistry (P<0.05).CONCLUSION: ERmRNA expression is related to the pathological behaviors of gastric carcinoma, which might help to predict the prognosis and predict the effectiveness of endocrine therapy for gastric carcinoma. | Xin-HanZhao Shan-ZhiGu Shan-XiLiu Bo-RongPan | 2003 | World Journal of Gastroenterology2003，9，4： | 36 |
| 3 | Growth,invasion,metastasis,differentiation,angiogenesis and apoptosis of gastric cancer regulated by expression of PTEN encoding products显示文摘AIM: To investigate expression of PTEN in gastric cancer and to explore its roles in tumorigenesis and progression of gastric cancer.METHODS: Formalin-fixed and paraffin-embedded tissues of adjacent non-tumor mucosa and primary foci from 113cases of gastric cancers were studied for the expression of PTEN and Caspase-3 andmicrovessel density (MVD)by streptavidin-peroxidase (S-P) immunohistochemistry with antibodies against PTEN, Caspase-3, and CD34. The relationship between PTEN and Caspase 3 expression and clinicopathological parameters of tumors was compared.RESULTS: Primary gastric cancer cells expressed PTEN less frequently than adjacent epithelial cells of primary foci (54.9% vs89.4%; P=0.000, χ2=33.474). PTEN expression was significantly associated with invasive depth (P=0.003,rs=0.274), metastasis (P=0.036, rs=0.197), growth pattern (P=0.008, rs=0.282), Lauren′s classification (P=0.000,rs=0.345), and histological classification (P=0.005, rs=0.262)of tumors, but not with tumor size (P=0.639, rs=0.045),Borrmann′s classification (P=0.544, rs=0.070) or TNM staging (P=0.172, rs=0.129). PTEN expression was negatively correlated with MDV in primary gastric cancer (P=0.020,F=5.558). Primary gastric cancer cells showed less frequent immunoreactivity to Caspase-3 than adjacent epithelial cells of primary foci (32.7 % vs 50.4 %; P=0.007,χ2=7.286).Caspase-3 expression was dependent of PTEN expression in primary gastric cancer cells (P=0.000, χ2=15.266).CONCLUSION: Down-regulated expression of PTEN plays an important role in tumorigenesis, progression, growth,differentiation and angiogenesis of gastric cancer. Low expression of PTEN can decrease expression of Caspase-3to disorder apoptosis of tumor cells, which might explain the molecular mechanisms of PTEN contributions to tumorigenesis and progression of gastric cancer. | Wei-GuoJiang Yin-ChangZhang YanXin Hua-ChuanZheng Yi-LingLi Jin-MinSun Xue-FeiYang Xiao-HanLi | 2003 | World Journal of Gastroenterology2003，9，8： | 33 |
| 4 | Detection of micrometastasis of gastric carcinoma in peripheral blood circulation显示文摘AIM:To detect the micrometastasis of gastric carcinoma in peripheral blood circulation using immunomagnetic beads sorting technique and RT-PCR technique,and to discuss its significance and the difference between the two methods.METHODS:Density gradient centrifugation was used to isolate mononuclear cells from peripheral blood,immunomagnetic beads sorting technique and RT-PCR technique were used to detect the disseminated carcinoma cells.HE,immunocytochemical and immunofluorescence staining were also used to identify the characteristics of the cells separated with immunomagnetic beads sorting technique.RESULTS:Cells expressing cytokeratin were separated and enriched from the peripheral blood specimens of patients suffering from gastric carcinoma or chronic gastritis. After HE staining, two kinds of ceils with little cytoplasm were found.Majority of these cells had small and round nuclei, even chromatins and the thickness of nuclear membrane was normal. Immunohistochemical staining indicated that there were CD34 and CD45 expression on the cell membrane ofthis kind of cells and these cells also showed expressed human telomerase reverse transcriptase by immunofluorescence staining, but the expression of carcinoembryonic antigen was absent.So,these cells might hematopoiesis precursors.Another kind of cells had larger and abnormal nuclei with thicker nuclear membranes. Massed chromatins and polynudeoli were found in the nudei. These cells expressed human telomerase reverse transcriptase and carcinoembryonic antigen,but CD34 and CD45 were not found on the cell membrane.So,these cells were considered as gastric carcinoma cells escaping from the original focuses and existing in the peripheral blood circulation.Cardnoma cells were found in 25 of 60(41.7%) specimens of peripheral blood from patients with gastric carcinoma, while there were no such cells separated from the blood specimens of chronic gastritis patients.The difference of positive rates of disseminated carcinoma cells between two groups was markedly significant (P<0.005).The expressions of CK20 mRNA in peripheral blood specimens were examinated withRT-PCR. CK20 mRNA was detected from 32 of 60(53.3%) peripheral blood specimens in the group of gastric carcinoma patients,while none of the specimens from patients suffering from chronic gastritis had CK20 mRNA. Significant difference was also found between two groups (P<0.005).Statistic analyses also showed that there was a significant difference between the positive rates of two methods in detecting the disseminated carcinoma cells from the peripheral blood circulation of gastric carcinoma patients (P<0.05).CONCLUSION:The results demonstrated that there were disseminated carcinoma cells in the peripheral blood circulation of some patients with gastric carcinoma.Disseminated carcinoma cells can be detected from the peripheral blood samples with immunomagnetic beads sorting technique and RT-PCR technique.The positive rate of RT-PCR technique is higher than that of immunomagnetic beads sorting technique in detecting micrometastasis. | Xi-MeiChen Guo-YuChen Zhi-RongWang Feng-ShangZhu Xiao-LeiWang XiaZhang | 2004 | World Journal of Gastroenterology2004，10，6： | 32 |
| 5 | Maspin expression and its clinicopathological significance in tumorigenesis and progression of gastric cancer显示文摘AIM:To investigate maspin expression in tumorigenesis and progression of gastric cancer and to explore its relevant molecular mechanisms.METHODS:Formalin-fixed and paraffin-embedded tissues from normal mucosa (n=182), dysplasia (n=69), cancer (n=l13) of the stomach were studied for maspin expression by immunohistochemistry. Microvessel density (MVD) in gastric cancer was labeled using anti-CD34 antibody. Maspin expression was compared with clinical parameters and MVD of tumors. Caspase-3 expression was also detected in gastric carcinoma by immunohistochemistry. The relationship between Caspase-3 and maspin expression was concerned as well.RESULTS:The positive rates of maspin expression were 79.8% (145/182), 75.4%(52/69) and 50.4%(57/113) in normal mucosa, dysplasia and cancer of the stomach,respectively.Cancer less frequently expressed maspin than normal mucosa and dysplasia (P<0.05).Maspin expression showed a significantly negative association with invasive depth, metastasis, Lauren's and Nakamura's classification (P<0.05),but not with tumor size, Borrmann's classification,growth pattern or TNIVl staging (P>0.05). The positive rate of Caspase-3 was significantly lower in gastric cancer than in normal gastric mucosa (P<0.05,32.7% vs 50.4%). It was noteworthy that maspin expression was negatively correlated with MVD, but positively correlated with expression of Caspase-3 in gastric cancer (P<0.05).CONCLUSION:Down-regulated maspin expression is a late molecular event in gastric carcinogenesis. Reduced expression of maspin contributes to progression of gastric cancer probably by inhibiting cell adhesion, enhandng cell mobility,decreasing cell apoptosis and facilitating angiogenesis.Additionally altered expression of maspin underlies the molecular mechanism of differentiation of gastric cancer and supports the different histogenetic pathways of intestinal and diffuse gastric cancers. Maspin expression can be considered as an effective and objective marker to reveal biological behaviors of gastric cancer. | Meng-ChunWang Yan-MinYang Xiao-HanLi FangDong YanLi | 2004 | World Journal of Gastroenterology2004，10，5： | 33 |
| 6 | Pathobiological behavior and molecular mechanism of signet ring cell carcinoma and mucinous adenocarcinoma of the stomach:A comparative study显示文摘AIM:To elucidate the distinctive pathobiological behavior between signet ring cell carcinoma (SRC) and mucinous adenocarcinoma of the stomach.METHODS: Based on the histological growth patterns and cell-functional differentiation classifications of stomach carcinoma, we conducted a series of comparative studies.All paraffin-embedded and frozen blocks were collected from the files of Cancer Institute of China Medical University. On the basis of histopathological observation, we applied enzymatic and mucous histochemistry, immunohistochemistry,flow cytometry (FCM) and molecular biology to compare these two categories of gastric cancers in terms of the DNA ploidy, proliferative kinetics, the expression of gastric carcinoma associated gene product and instabilities of mitochondrial DNA (mtDNA).RESULTS:Gastric SRC was commonly seen in females below 45 years, mostly presenting diffuse growth and ovary or uterine cervix metastasis. The majority of SRC were absorptive and mucus-producing functional differentiation type (AMPFDT), which growth relied on estrogen. Meanwhile,stomach mucinous adenocarcinomas were mostly observed in males over 50 years, prone to massive growth or nest growth and extensive peritoneal infiltration, showing two categories of cell-functional differentiation types: AMPFDT and mucus-secreting functional differentiation type (MSFDT).Expressions of ER, enzyme c-PDE and 67kDaLN-R in SRC were evidently higher than that in mucinous adenocarcinoma,while expressions of LN, CN-Ⅳ, CD44v6, and PTEN protein were obviously lower in SRC than that in mucinous adenocarcinoma (P<0.05).There was no statistic significance in VEGF, ECD and instabilities of mtDNA (P>0.05) between the above two gastric carcinomas.CONCLUSION: Though SRC and mucinous adenocarcinoma were both characterized by abundant mucus-secretion, they were quite different in morphology, ultrastructure, cell-functional differentiation and protein expression, indicating different mechanisms of carcinogenesis. We concluded that combining histological growth patterns, cell-functional differentiation type with tumor related markers might be significant in early diagnosis and prognosis assessment for SRC and mucinous adenocarcinoma of the stomach. | Xue-FeiYang LinYang Xiao-YunMao Dong-YingWu Su-MinZhang YanXin | 2004 | World Journal of Gastroenterology2004，10，5： | 33 |
| 7 | Expression of nuclear factor-kappa B and target genes in gastric precancerous lesions and adenocarcinoma:Association with Helicobactor pylori cagA (+) infection显示文摘AIM:To examine the expression of nuclear factor kappaB (NF-κB) and its target genes in intestinal metaplasia (IM),dysplasia (DYS) and gastric carcinoma (GC) infected with Helicobacter pylori (H pylori) and to investigate the mechanism underlying Hpyloricytotoxin associated gene A(cag A) infection leading to gastric adenocarcinoma.METHODS: Expressions of NF-κB/p65 and its target genes:c-myc, cyclinD1 and bcl-xl were immunohistochemically examined in 289 cases of gastric biopsy and resection specimens from patients with IM, DYS and GC infected with H pylori. H pylori in the above mentioned tissues was detected by Warthin-Starry stain and rapid urease tests.IgG antibody to cagA in sera of the patients was measured by ELISA.RESULTS:The positive rates of NF-κB/p65 were significantly higher in groups with cagA of IMI-Ⅱ(28/33), IM III(48/52),DYSI(27/31), DY5 Ⅱ-Ⅲ(28/32), GC(35/40) than in groups without cagA of IMI-Ⅱ(4/17), IMⅢ(3/20), DYSI(3/20),DYSⅡ-Ⅲ(6/21), GC(10/23). The expressions of c-myc,cyclinD1, and bcl-xl were significantly higher in groups with cagA of IM Ⅲ(47/52, 49/52, 46/52), DYSⅡ-Ⅲ(29/32, 26/32,25/32) than in groups without cagA of IM Ⅲ(8/20, 7/20,5/20), DYSⅡ-Ⅲ(10/21, 8/21, 3/21), which were in conformity with the expression of NF-κB in IM Ⅲ, and DYSⅡ-Ⅲ. Asignificantly higher expression level of NF-κB/p65, c-myc,cyclinD1 and bcl-xl was detected in intestinal type GC(27/28,18/28, 22/28, 24/28) than in diffuse type GC(8/12, 3/12,3/12, 6/12), respectively.CONCLUSION: There may be two different molecular mechanisms in the occurrence of intestinal and diffuse type gastric carcinomas. Intestinal type gastric carcinoma is strongly associated with high expression of c-myc, cyclinD1 and bcl-xl through NF-κB/p65 activated by Hpylori cagA.Inhibiting the activity of NF-κB is an effective and promising way to prevent intestinal type gastric carcinoma. | Gui-FangYang Chang-ShengDeng Yong-YanXiong Ling-LingGong Bi-ChengWang JunLuo | 2004 | World Journal of Gastroenterology2004，10，4： | 27 |
| 8 | 胃印戒细胞癌与黏液腺癌生物学特征及分子病理学机制的比较研究显示文摘目的:以胃癌组织学生长方式分型和癌细胞功能分化表型研究为基础,从形态、机能与蛋白分子水平相结合的层次,探讨胃印戒细胞癌和黏液腺癌浸润转移的生物学规律及相关分子病理学机制。 方法:本系列研究标本全部取自中国医科大学肿瘤研究所存档石蜡标本和冰冻标本。在病理形态学观察的基础上,采用酶组织化学、黏液组织化学和免疫组织化学、流式细胞技术(FCM)结合分子生物学方法检测并比较上述两型胃癌相关基因编码蛋白表达、癌细胞DNA倍体、增生细胞动力学,微卫星不稳定性和杂合性丢失等分子生物学特征。 结果:近40a研究发现,胃印戒细胞癌好发于中青年女性,组织学上倾向于弥漫性生长,以吸收-黏液分泌功能双向分化(AMPFDT)为主,生长依赖雌激素,好发卵巢和子宫颈转移。胃黏液腺癌多见于50岁以上男性,倾向于团块状或巢状生长,功能分化上表现为AMPFDT型和黏液分泌功能分化型(MSFDT)两种类型,生长不依赖雌激素,以腹膜广泛浸润生长为主要特点,层粘蛋白及其受体和Ⅳ型胶原、CD_(44)v_6、PTEN蛋白表达显著高于胃印戒细胞癌。两型胃癌酸性磷酸酶(ACP)和血管内皮生长因子(VEGF)无显著差异。 结论:胃印戒细胞癌和黏液腺癌虽都以旺盛的黏液分泌能力为主要特征,但他们在病理形态、超微结构、生长浸润方式、功能分化和基? | 杨琳 王艳萍 吴东瑛 张素敏 李锦毅 张荫昌 辛彦 | 2002 | 世界华人消化杂志2002，10，5： | 24 |
| 9 | Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis-9,trans-11-conjugated linoleic acid显示文摘AIM: To determine the effect of apoptosis on gastric cancer cells (SGC-7901) induced by cis-9, trans-11-conjugated linoleic acid (c9, t11-CLA) and its possible mechanism in the inhibition of cancer cells growth.METHODS: Using cell culture, flow cytometery and immunocytochemical techniques, we examined the cell growth, frequency of apoptosis and distribution of cell cycle,expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25,50,100 and 200 μmol@L-1) of c9, t11-CLA for 24h and 48 h,with a negative control (0.1% ethanol).RESULTS: The growth of SGC-7901 cells was inhibited by c9,t11-CLA. Eight days after treatment with various concentrations of c9,t11-CLA, as mentioned above, the inhibition rates were 5.9 %, 20.2 %,75.6 % and 82.4 %, respectively. The frequency of apoptosis on SGC-7901 cells induced by different concentrations of c9, t11-CLA (except for 25 μmol@L-1, 24 h) was significantly greater than that in the negative control (P<0.01). To further investigate the influence of the cell cycle progression, we found that apoptosis induced by c9, t11-CLA may be involved in blocking the cell cycle of SGC-7901 cells. Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations for various time periods significantly decreased the expressions of ki67 (the expression rates were 18.70-3.20 %, at 24 h and 8.10-0.20 % at 48 h, respectively), bd-2 (4.30-0.15 % at 24 h and 8.05 %-0 at 48 h),and c-myc(4.85-2.20 % at 24 h and 4.75-0.30 % at 48 h) as compared with those in the controls (the expressions of ki67, bcl-2, and c-mycwere 15.1% at 24 h and 13.5 % at 48 h, 6.80 % at 24 h and 8.00 % at 48 h,5.50 % at 24 h and 5.30 % at 48 h, respectively) (P<0.01),whereas the expressions of Fas were increased (0.60-2.75 %,24 h and 0.45-5.95 %, 48 h).CONCLUSION: The growth and proliferation of SGC-7901 cells are inhibited by cg, t11-CLA via blocking the cell cycle,pathways of bcl-2-associated mitochondria with reduced expression of bcl-2 and Fas-associated death domain protein (FADD) with enhanced expression of Fas. But expression of c-myc on SGC-7901 cells is lower than that in negative control, which needs to be studied further. | Jia-RenLiu Bing-QingChen Yan-MeiYang Xuan-LingWang Ying-BenXue | 2002 | World Journal of Gastroenterology2002，8，6： | 20 |
| 10 | Variations of mitochondrial D-loop region plus downstream gene 1 2S rRNA-tRNA^(phe) and gastric carcinomas显示文摘AIM: To explore the instabilities, polymorphisms and other variations of mitochondrial D-loop region and downstream gene 12S rRNA-tRNAPhe in gastric cancers, and to study their relationship with gastric cancer.METHODS: Three adjacent regions (D-loop, tRNAphe and 12S rRNA) were detected for instabilities, polymorphisms and other variations via PCR amplification followed by direct DNA sequencing in 22 matched gastric cancerous tissues and para-cancerous normal tissues.RESULTS: PolyC or (CA)n instabilities were detected in 13/22(59.1%) gastric cancers and 9/22(40.9 %) in the control (P>0.05). There existed 2/12(16.7%) and 6/10(60%)alterations of 12S rR NA-tRNAphe in well differentiated gastric cancers and poorly differentiated ones, respectively(P0.05).Some new variations were found, among which np 318 and np 321 C-T transitions in D-loop region were two of the five bases for H-strand replication primer. Np 523 AC-deletion and np 527 C-T transition occurred at mtTF1 binding site (mtTFBS), which were associated with the transcription of downstream mitochondrial genome. Seven samples showed the np 16 182 polyC instabilities, five of which simultaneously showed np 16 189 T-C transitions.CONCLUSION: There is no statistic significance of instabilities and polymorphisms in mitochondrial D-loop region between gastric cancerous and para-cancerous normal tissues, which suggests that the instability might relate to heredity or be dependent on aging. There is asignificant correlation between differentiation degree of gastric cancer and variant frequencies of 12S rRNA-tRNAphe. The poorly differentiated gastric cancers are more prone to 12S rRNAtRNAphe variations, or gastric cancers with 12S rRNA-tRNAphe variations are more likely to be poorly differentiated, np 16189 T-C transition may be one of the important reasons for polyC instability in gastric cancer. | Cheng-BoHan FanLi Yu-JieZhao Jia-MingMa Dong-YingWu Yu-KuiZhang YanXin | 2003 | World Journal of Gastroenterology2003，9，9： | 20 |
| 11 | 胃癌线粒体DNA拷贝量的变化显示文摘目的:通过比较线粒体基因组(mitochondrialDNA,mtDNA)拷贝数在胃癌和癌旁胃黏膜组织间的差异,阐述mtDNA与胃癌发生的关系.方法:PCR分别扩增胃癌组织和癌旁胃黏膜组织各20例共40个样本的线粒体D-loop两个高变区HV1(hypervariableregion)和HV2;并以核基因组的β-actin作为定量标准物.聚丙烯酰胺凝胶电泳(polyacrylamidegelelectrophoresis,PAGE)银染比较mtDNA拷贝数在癌和正常组织间的差异.结果:HV1和HV2拷贝量(用β-actin标准化)在胃癌组织和癌旁组织间有显著的差异(P<0.01);其拷贝量与组织类型,癌组织浸润深度未发现有统计学联系(P>0.05);而与核内一些重要的酶:碱性磷酸酶(AKP)、环腺苷酸磷酸二脂酶(cAMP-PDE)和环鸟苷酸磷酸二脂酶(cGMP-PDE)表达有一定关系(P<0.05).结论:胃癌的发生与胃上皮细胞内mtDNA量的减少有着密切的关系.有望成为一种新的肿瘤分子标志物. | 韩琤波 李凡 杨雪飞 毛晓韵 吴东瑛 辛彦 | 2004 | 世界华人消化杂志2004，12，2： | 16 |
| 12 | 胃癌转移规律研究新进展显示文摘胃癌转移方式按好发程度依次为淋巴结转移、腹膜种植转移和血行转移。淋巴结转移的高危因素包括浸润深度,大体类型,生长方式,癌灶长径>4cm,低分化,淋巴管受侵阳性等。有无淋巴结转移是影响早期胃癌预后最重要的独立危险因素。术中腹腔冲洗液脱落癌细胞(ECC)检查是诊断或检测潜在腹膜转移的常用方法和金标准。不同的浆膜分型是预测胃癌根治术后腹膜复发的独立危险因素。CEA、肝素酶等肿瘤标记物对检测或预测腹膜转移具有较好的临床意义。胃癌的血行转移多发生于肝、肺、骨等脏器。隆起型、高分化、AFP阳性的肝样腺癌、团块状生长、静脉癌栓阳性病例易发生肝转移。浸润型、低分化、静脉癌栓阳性是胃癌肺、骨转移的病理生物学特征。 | 徐惠绵 徐岩 | 2011 | 中国实用外科杂志2011，31，8： | 15 |
| 13 | Mast cell density and the context of clinicopathological parameters and expression of p185,estrogen receptor,and proliferating cell nuclear antigen in gastric carcinoma显示文摘AIM: To investigate the relationship between the mast cell density (MCD) and the context of clinicopathological parameters and expression of p185, estrogen receptor (ER), and proliferating cell nuclear antigen (PCNA) in gastric carcinoma.METHODS: Mast cell, p185, ER, and PCNA were detected using immunohistochemical S-P labeling method. Mast cell was counted in tissue of gastric carcinoma and regional lymph nodes respectively, and involved lymph nodes (TLN) were examined as usual.RESULTS: MCD was significantly related to both age and depth of penetration (χ2=4.688,P<0.05 for age and χ2=9.350,P<0.01 for depth of penetration) between MCD>21/0.03 mm2 and MCD≤21/0.03 mm2 in 100 patients; MCD in 1-6 ILN group patients was significantly higher than that in 7-15 TLN or >15 TLN group patients (u=6.881, 8.055, P<0.01);There were significant differences intergroup in positive expression rate of p185, ER and PCNA between MCD >21/0.03 mm2 and MCD≤21/0.03 mm2 in 100 patients.CONCLUSION: Mast cell may have effect on inhibiting invasive growth of tumor, especially in the aged patients; The number of mast cells, in certain degree, may predicate the number of involved lymph nodes, which is valuable for assessment of prognosis; MCD was related to the expression of p185, ER, and PCNA in gastric carcinoma. Tt suggests that mast cell accumulation may inhibit the proliferation and the dissemination of the gastric carcinoma. | Ying-AnJiang You-YuanZhang He-ShengLuo Shou-FuXing | 2002 | World Journal of Gastroenterology2002，8，6： | 13 |
| 14 | Expression of Fas ligand and Caspase-3 contributes to formation of immune escape in gastric cancer显示文摘AIM: To study the role of Fas ligand (FasL) and Caspase-3expression in carcinogenesis and progression of gastric cancer and molecular mechanisms of relevant immune escape.METHODS: FasL and Caspase-3 expression was studied in adjacent epithelial cells, cancer cells and lymphocytes of primary foci, and cancer cells of metastatic foci from 113 cases of gastric cancer by streptavidin-biotin-peroxidase (S-P) immunohistochemistry. Expression of both proteins in cancer cells of primary foci was compared with clinicopathological features of gastric cancer. The relationship between FasL expression in cancer cells and Caspase-3expression in cancer cells or infiltrating lymphocytes of primary foci was investigated.RESULTS: Cancer cells of primary foci expressed FasL in 53.98 % (61/113) of gastric cancers, more than their adjacent epithelial cells (34.51%, 39/113) (P=0.003, X2=8.681), while the expression of Caspase-3 in cancer cells of primary foci was detected in 32.74 % (37/113) of gastric cancers, less than in the adjacent epithelial cells (50.44 %, 57/113)(P=0.007, X2=7.286). Infiltrating lymphocytes of the primary foci showed positive immunoreactivity to Caspase-3 in 70.80 % (80/113) of gastric cancers, more than their corresponding adjacent epithelial cells (P=0.001, X2=10.635)or cancer cells of primary foci (P=0.000, X2=32.767). FasL was less expressed in cancer cells of metastases (51.16 %,22/43) than in those of the corresponding primary foci (81.58 %, 31/38) (P=0.003, X2=9.907). Conversely,Caspase-3 was more expressed in cancer cells of metastases (58.14 %, 25/43) than in those of the corresponding primary foci (34.21%, 13/38) (P=0.031, X2=4.638). FasL expression was significantly correlated with tumor size (P=0.035,rs=0.276), invasive depth (P=0.039, rs=0.195), metastasis (P=0.039, rs=0.195), differentiation (P=0.015, rs=0.228)and Lauren′s classification (P=0.038, rs=0.196), but not with age or gender of patients, growth pattern or TNM staging of gastric cancer (P>0.05). In contrast, Caspase-3 expression showed no correlation with any dinicopathological parameters described above in cancer cells of the primary foci (P>0.05).Interestingly, FasL expression in primary gastric cancer cells paralleled to Caspase-3 expression in infiltrating lymphocytes of the primary foci (P=0.016, X2=5.825).CONCLUSION: Up-regulated expression of FasL and downregulated expression of Caspase-3 in cancer cells of primary foci play an important role in gastric carcinogenesis. As an effective marker to reveal the biological behaviors, FasL is implicated in differentiation, growth, invasion and metastasis of gastric cancer by inducing apoptosis of infiltrating lymphocytes. Chemical substances derived from the primary foci and metastatic microenvironment can inhibit the growth of metastatic cells by enhancing Caspase-3 expression and diminishing FasL expression. | Jin-MinSun Zheng-LiWei Xue-Feiyang Yin-ChangZhang YanXin Hua-ChuanZheng | 2003 | World Journal of Gastroenterology2003，9，7： | 13 |
| 15 | Effect of P-selectin monoclonal antibody on metastasis of gastric cancer and immune function显示文摘AIM: To investigate the effect of cell adhesion molecule Pselectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue.METHODS: SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 μg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the 3H TdR infiltration method, the NK cell activity was detected by the LDH release method.RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time.The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by Pselectin Mab.CONCLUSION: P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function. | Jin-Lian Wei-xiongChen Jin-ShuiZhu Ni-WeiChen TongZhou Yun-LinWu MingYao Dong-QingZhang | 2003 | World Journal of Gastroenterology2003，9，7： | 12 |
| 16 | Expression of PCNA and CD44mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis显示文摘AIM: To investigate the expression of proliferating cell nuclear antigen (PCNA) and CD44mRNA in colorectal cancer with venous invasion and its relationship with liver metastasis.METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of PCNA and CD44mRNA in 31 cases of colorectal cancer with venous invasion.RESULTS: Positive expression rates of PCNA and CD44mRNA in colorectal cancer were higher than those without liver metastasis (P<0.05 and P<0.01). In case of colorectal cancer with liver metastasis, strongly positive rates of PCNA and CD44mRNA were 94.1% and 70.6 %,respectively, significantly higher than those without liver metastasis. There was a positive relationship between the expressions of PCNA and CD44mRNA (r=0.67, P<0.05).CONCLUSION: Detection of PCNA and CD44mRNA expression in colorectal cancer may be useful for evaluating liver metastasis of cancer cells. | Shu-Qiang Yue Yan-Ling Yang Ke-Feng Dou Kai-Zong Li Department of Hepatobiliary Surgery,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 11 |
| 17 | Expression of TFF2 and Helicobacter pylori infection in carcinogenesis of gastric mucosa显示文摘AIM: To investigate the expression of TFF2 and Helicobacter pyloriinfection in carcinogenesis of gastric mucosa.METHODS: The expression of TFF2 was immunohistochemically analyzed in paraffin-embedded samples from 119 patients with endoscopic biopsy and subtotal gastrectomy specimens of gastric mucosal lesions, including 16 cases of chronic superficial gastritis (CSG), 20 chronic atrophic gastritis (CAG),35 intestinal metaplasia (IN), 23 gastric epithelial dysplasia (GED) and 25 gastric carcinoma (CA), and Helicobacter pylori infection was detected by Warthin-Starry staining.RESULTS: 1:TFF2 was located in the cytoplasm of gastrk mucous neck cell. The expression of TFF2 was 100 %,100 %, 0, 56.5 % and 0 in CSGs, CAGs, INs, GEDs and CAs, respectively. 2: The value of TFF2 positive cell density in CSG with Helicobacter pyloriinfection was higher than that without Helicobacter pyloriinfection. (52.89±7.27vs46.49±13.04, P>0.05); But the value of TFF2 positive cell density in CAG and GED with Helicobacter pyloriinfection was significantly lower than that without Helicobacter pylori infection (18.17±4.09 vs 37.93±13.80, P<0.01 and 14.44±9.32 vs 24.84±10.22, P<0.05).CONCLUSION: Increase of TFF2 expression in CSG is perhaps associated with the protective mechanism after gastric mucosal injury. Decrease of TFF2 expression in CAG possibly attributes to the decrease in the number of gastric gland cell expressing TFF2. Re-expression of TFF2 in gastric epithelial dysplasia implies that TFF2 possibly contributes to the initiation of gastric carcinoma. The effect of Helicobacter pylori on the expression of TFF2 depends on the status of gastric mucosa. | Guo-Yong Hu Bao-Ping Yu Wei-Guo Dong Mu-Qi Li Jie-Ping Yu He-Sheng Luo Zong-Xue Rang Gastroenterology Department,Renmin Hospital of Wuhan University,Wuhan 430060,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 10 |
| 18 | 胃癌组织CD_(44)v9和MMP-2基因的表达显示文摘目的:研究CD_(44)v9和MMP-2在胃癌和癌旁组织中的表达,探讨胃癌侵袭与转移的可能机制。方法:采用RT-PCR方法分别检测40例胃癌及癌旁组织CD_(44)v9及MMP-2的阳性表达情况。结果:40例胃癌组织中CD_(44)v9和MMP-2的阳性表达率分别为75%和82.5%,明显高于癌旁组织的35%和48.5%,二者比较差异均有显著性(x^2=12.929;x^2=10.769.P均≤0.001)。CD_(44)v9和MMP-2表达与肿瘤的大小、分化高低、浸润深度及临床分期有关,合并有淋巴结转移的17例胃癌患者CD_(44)v9和MMP-2表达率明显高于不伴有淋巴结转移的胃癌患者(P均<0.05),CD_(44)v9和MMP-2的表达与胃癌有相关性(r=0.6,P<0.001)。结论:CD_(44)v9和MMP-2与胃癌侵袭和转移性有关,可作为预测肿瘤转移潜能的指标。 | 张翠萍 田字彬 赵清喜 武军 梁永信 | 2003 | 世界华人消化杂志2003，11，10： | 9 |
| 19 | 基质金属蛋白酶-7表达与胃癌临床病理生物学行为的关系显示文摘目的:研究基质金属蛋白酶-7(MMP-7)表达在胃癌发生和演进中的作用。方法:采用S-P免疫组化方法利用抗MMP-7、抗CD34抗体研究 113例胃癌原发灶中 MMP-7表达和微血管密度(MVD),比较 MMP-7 表达和 MVD与胃癌临床病理特征的关系,分析胃癌组织中MMP—7表达与MVD的关系。结果:MMP-7在胃癌旁黏膜中阳性表达率为29.2%(33/113),低于胃癌组织的阳性率 69.0%(78/113);MMP-7表达与胃癌肿块大小、浸润深度、转移和TNM 分期密切相关(P<0.05),而与胃癌的生长方式和分化程度无显著相关性(P>0.05);胃癌组织中MVD与肿块大小、浸润深度、转移和TNM分期关系密切(P<0.05),但与胃癌的组织分化和生长方式无显著相关性(P>0.05);特别是MVD的大小依赖于胃癌组织中 MMP—7表达(P<0.05)。结论:MMP-7在胃癌中表达上调,可作为揭示胃癌生物学行为的客观指标,其可能通过参与胃癌的生长、浸润、转移和血管形成而在胃癌的发生和演进中起到重要作用。 | 孙晋民 郑华川 杨雪飞 辛彦 张荫昌 | 2003 | 世界华人消化杂志2003，11，9： | 6 |
| 20 | Apoptosis of human gastric adenocarcinoma cells induced by β-ionone显示文摘AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.METHODS: Using M-IT, fluorescence dye (Hoechst-33258),transmission electron microscopy and the TUNEL assay,we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200μmol/L) for 24h,48h.RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%,78.25% and 94.15%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION:β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells.However, the mechanism needs to be further investigated. | Jia-RenLiu 3ing-QingChen Bao-FengYang Hong-WeiDong Chang-HaoSun Qiwang GuoSong You-Qiangsong | 2004 | World Journal of Gastroenterology2004，10，3： | 6 |
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