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| 1 | 断肠草资源的开发与应用显示文摘断肠草(Gelsemium elegansBenth)是一种有毒植物,主要含有多种吲哚类生物碱,近年来在临床上广泛用于抗肿瘤、镇痛及免疫调节等方面.文章对断肠草在医药、农业、畜牧业等方面的应用进展进行了综述,并对其在应用中存在的问题及发展前景进行了讨论和展望. | 陈志慧 于新 宋光泉 | 2006 | 仲恺农业技术学院学报2006，19，2： | 9 |
| 2 | 食细菌线虫Caenorhabditis elegans的取食偏好性显示文摘通过设置平板培养试验,以模式种线虫Caenorhabditis elegans为材料,观察了食细菌线虫的取食行为。结果表明:C.elegans在取食细菌时对原位土壤中分离的一种Pseudomonas sp细菌存在最大的取食偏好性。这种取食偏好性表现在大部分C.elegans在24 h内都直接朝Pseudomonas sp迁移,说明C.elegans能通过某种机制识别Pseudomonas sp。距离迁移试验及C.elegans迁移率表明它可能是通过辨别细菌所发出的气味识别它喜欢的食物。C.elegans的繁殖率跟其取食的偏好性是相关的,在迁移率较高的细菌培养基中线虫表现出更高的繁殖率。结果还表明:相对于G+细菌而言,C.elegans偏好取食G-细菌。为进一步了解土壤生态系统中食细菌线虫与细菌群落结构间相互关系提供了帮助。 | 肖海峰 焦加国 胡锋 李辉信 | 2010 | 生态学报2010，30，24： | 5 |
| 3 | Anti-parasitic effects of water-soluble alkaloid fractions from ethanolic extracts of Sophora moorcroftiana seeds in Caenorhabditis elegans显示文摘Parasite infections of humans and animals remain a major global health problem, with limited choice of drugs being available to the treatment of parasitosis in the clinic. Sophora moorcroftiana(S. moorcroftiana) is a shrub that grows in Tibet Plateau of China. Decoction of the seeds has been used as a traditional Tibetan medicine to treat parasitosis for years. But the anti-parasitic effects of water-soluble fractions in the seeds need further investigation. In the present study, the water-soluble alkaloid fractions(E2) were obtained from S. moorcroftiana seeds by refluxing extraction with 60% ethanol and low polarity fraction(E2-a) and high polarity fraction(E2-b) were subsequently isolated from E2 using column chromatography. As a parasite model, Caenorhabditis elegans(C. elegans) were treated with different fractions and their survivals were recorded. The results showed that that E2-a induced a lower survival rate in C. elegans than E2-b and E2. The protoscoleces of Echinococcus granulosus(E. granulosus) were cultured in the presence of E2-a. Compared with E2-b and E2, protoscoleces exhibited decreased survival rate following E2-a treatment. Furtherly, the effects of E2-a on the behavior, brood size, and lifespan of the worms were investigated. Body bend frequencies of the worms treated with the high concentration of E2-a were reduced by two-thirds compared with the control group(P < 0.01). Compared with non-E2-a-treated group, exposure of nematodes to E2-a led to a decrease in head thrashes and pharyngeal pumps frequency(P < 0.01). E2-a treatment resulted in a significantly lower brood size(P < 0.01). Additional E2-a treatment induced a significantly shortened lifespan, compared with the control(P < 0.05). These findings indicated that water-soluble fraction E2-a from S. moorcroftiana seeds was a potential helminthic agent. | LUO Yan-Ping ZHANG Yuan ZHANG Hui-Min ZHANG Hong ZHANG Lin YU Hong-Juan CAO Ming-Qiang SHI Yan-Bin ZHI De-Juan MA Xing-Ming DONG Kai-Zhong | 2018 | Chinese Journal of Natural Medicines2018，16，9： | 3 |
| 4 | 高功率毫米波辐照对秀丽线虫行为学和细胞形态学的影响显示文摘采用角锥喇叭天线,使用不同输出平均功率的毫米波激光辐照秀丽线虫,采用实时显微摄像系统记录线虫行为学变化,并使用体视显微镜观察线虫细胞形态学变化。受照射后自由活动的秀丽线虫能够快速逃离高功率毫米波的辐照区域。当受输出平均功率为10 W和12 W辐照后,线虫先后出现运动速度增加、运动速度减慢直至身体僵直不动的变化过程。当输出平均功率为5 W时,线虫出现热耐受现象。细胞形态学观察结果显示:辐照后的线虫腹中卵细胞核结构出现不同程度的异常。高功率毫米波辐照可显著影响线虫的行为学特征及细胞形态学,同时说明秀丽线虫是可适用于高功率毫米波生物效应研究的模式生物之一。 | 任长虹 袁广江 高艳 吴永红 徐志伟 徐寿喜 粟亦农 刘濮鲲 张成岗 | 2010 | 强激光与粒子束2010，22，12： | 2 |
| 5 | 线虫脂肪沉积中组织蛋白酶B的表达及功能研究显示文摘文章利用高糖/高胆固醇饮食诱导了线虫,油红O染色结果表明线虫脂肪沉积显著增加。为了研究组织蛋白酶B在诱导线虫过程中的作用,利用实时荧光定量聚合酶链式反应(polymerase chain reaction,PCR)研究了组织蛋白酶B同源基因的表达。选取其中的关键基因利用RNA干扰(RNA interference,RNAi)技术,研究其在线虫脂肪沉积中的作用。结果表明:在线虫脂肪沉积改变后,组织蛋白酶B同源基因表达随之改变;而利用RNA干扰组织蛋白酶B关键基因cpr-3的表达,线虫的脂肪沉积未受影响。因此,组织蛋白酶B同源基因可能参与线虫脂肪沉积改变后的生理变化,而未直接参与线虫脂肪沉积的调控。 | 赵丽娟 鲍斌 | 2016 | 合肥工业大学学报(自然科学版)2016，39，6： | 2 |
| 6 | C.elegans-based screen identifies lysosome-damaging alkaloids that induce STAT3-dependent lysosomal cell death显示文摘Lysosomes are degradation and signaling centers within the cell,and their dysfunction impairs a wide variety of cellular processes.To understand the cellular effect of lysosome damage,we screened natural smallmolecule compounds that induce iysosomal abnormality using Caenorhabditis elegans (C.elegans)as a model system.A group of vobasinyl-ibogan type bisindole alkaloids (ervachinines A-D)were identified that caused lysosome enlargement in C.elegans macrophage-like cells.Intriguingly,these compounds triggered cell death in the germ line independently of the canonical apoptosis pathway.In mammalian cells, ervachinines A-D induced lysosomal enlargement and damage,leading to leakage of cathepsin proteases, inhibition of autophagosome degradation and necrotic cell death.Further analysis revealed that this ervachinine-induced lysosome damage and lysosomal cell death depended on STAT3 signaling,but not RIP1 or RIP3 signaling.These findings suggest that lysosomedamaging compounds are promising reagents for dissecting signaling mechanisms underlying lysosome homeostasis and lysosome-related human disorders. | Yang Li Yu Zhang Qiwen Gan Meng Xu Xiao Ding Guihua Tang Jingjing Liang Kai Liu Xuezhao Liu Xin Wang Lingli Guo Zhiyang Gao Xiaojiang Hao Chonglin Yang | 2018 | Protein & Cell2018，9，12： | 2 |
| 7 | 植物生长调节剂对百日草生长及开花的影响显示文摘以百日草(Zinnia elegans Jacq.)为试材,采用不同浓度的赤霉素(GA3)、油菜素内酯(BR)和不同稀释倍数的的花粉酵素对百日草进行2次全株喷施处理,探讨对百日草花期及开花质量的影响。结果表明,在一定范围内喷施赤霉素可使百日草开花提前、延长花期,而喷施油菜素内酯、花粉酵素可不同程度地推迟百日草开花;三者均可促进花径、叶面积、株高、单株花朵数的增加,提高百日草的观赏价值。综合考虑,3种植物生长调节剂中以0.02 mg/L的油菜素内酯喷施处理的效果最好;其次是花粉酵素800倍稀释液处理;赤霉素喷施浓度以100 mg/L为宜。 | 孙晨 王克凤 董然 钱英 孙晓丹 李春鹏 | 2018 | 湖北农业科学2018，57，4： | 2 |
| 8 | C.elegans野生型和par突变体早期胚胎卵裂的观察显示文摘不对称分裂在动植物的发育中起到了非常重要的作用。Caenorhabditiselegans(C.elegans)胚胎最早的两次卵裂为研究控制不对称分裂的机制提供了很好的机会。用普通光学显微镜观察了野生型胚胎早期卵裂和par-1、par-2、par-3、par-4突变体胚胎的早期卵裂。野生型胚胎最早的分裂是不等的,产生了两个不同大小的子细胞。两个子细胞又以不同的方向进行第二次分裂。在C.elegans中任意一个par基因的缺失会使胚胎的第一次卵裂丧失不对称性。这会导致一些发育调控因子不能在特定的胚胎细胞中准确地定位,造成细胞分裂纺锤体方向的异常。par类基因参与不对称性的建立,这种不对称性决定了C.elegans身体的前后轴。 | 傅兴 杨玉荣 | 2005 | 细胞生物学杂志2005，27，6： | 2 |
| 9 | 外源水杨酸对百日草生长及开花的影响显示文摘为探讨外源水杨酸(SA)对百日草(Zinnia elegans Jacq.)生长发育的影响,以梦境百日草为试验材料,研究了不同浓度SA对百日草生长及开花的影响.结果表明,0.5mmol/L水杨酸处理显著提高百日草的株高、生物量、真叶数及开花数量,1.0mmol/L处理与对照相比大多差异不显著.综合来看,适宜浓度的外源SA对百日草的生长及开花有明显的促进作用. | 相程程 朱彦霖 魏敏 戴晓玲 王晓彤 李海云 | 2019 | 湖北农业科学2019，58，17： | 1 |
| 10 | 杀线虫活性菌的筛选、分离与初步鉴定显示文摘从河南南阳宝天曼森林土壤样品中分离纯化细菌,以对线虫Caenorhabditis elegans的杀灭作用为筛选模型,从128株细菌中共筛选到3株杀线虫活性较高的细菌,编号分别为B、C、D。当作用于线虫24 h后,它们对线虫的致死率分别为20%、25%、97%。然而发现当B和C共同作用于线虫时,致死率大为提高(约为90%),说明二者在杀线虫方面有协同作用。通过培养观察菌落形态,革兰氏染色;提取各菌种基因组,扩增16S rDNA测序,系统发育分析,确定这三株菌的鉴定结果为:菌株B与Bacillus subtilis strain xfchu2的同源性较高,为96.46%,菌株C与Stenotrophomonas maltophilia strain QT24的同源性较高,为92.75%。菌株D与荧光假单胞菌属的同源性较高。 | 张林 牛秋红 周晓丽 | 2012 | 南阳理工学院学报2012，4，2： | 1 |
| 11 | A STUDY OF LICHENOMETRY AND ITS APPLICATION TO SOME GEOLOGICAL EVENTS IN SHAANXI, CHINA显示文摘In 1950, R. E. Beschel proposed the lichenometric dating technique. For age determination, the diameter growth rate of lichens is usually taken as a constant. Alternatively, the growth curve of lichens is derived by dating objects of known age in some area, and it can then be used for age determination in the same area or areas of similar environment. Unfortunately it is very difficult to obtain such growth curves in many places. The growth curves differ in different environments. In order to use this technique under all conditions, the relationship between the lichen growth and the environment has to be investigated. | 谢新生 肖振敏 | 1990 | Chinese Science Bulletin1990，35，15： | 1 |
| 12 | Expression of hepatitis B virus surface antigens induces defective gonad phenotypes in Caenorhabditis elegans显示文摘AIM To test whether a simple animal, Caenorhabditis elegans(C. elegans), can be used as an alternative model to study the interaction between hepatitis B virus antigens(HBs Ag) and host factors. METHODS Three plasmids that were able to express the large, middle and small forms of HBs Ags(LHBs Ag, MHBs Ag, and SHBs Ag, respectively) driven by a ubiquitous promoter(fib-1) and three that were able to express SHBs Ag driven by different tissue-specific promoters were constructed and microinjected into worms. The brood size, egglaying rate, and gonad development of transgenic worms were analyzed using microscopy. Levels of m RNA related to endoplasmic reticulum stress, enpl-1, hsp-4, pdi-3 and xbp-1, were determined using reverse transcription polymerase reaction(RT-PCRs) in three lines of transgenic worms and dithiothreitol(DTT)-treated wild-type worms. RESULTS Severe defects in egg-laying, decreases in brood size, and gonad retardation were observed in transgenic worms expressing SHBs Ag whereas moderate defects were observed in transgenic worms expressing LHBs Ag and MHBs Ag. RT-PCR analysis revealed that enpl-1, hsp-4 and pdi-3 transcripts were significantly elevated in worms expressing LHBs Ag and MHBs Ag and in wild-type worms pretreated with DTT. By contrast, only pdi-3 was increased in worms expressing SHBs Ag. To further determine which tissue expressing SHBs Ag could induce gonad retardation, we substituted the fib-1 promoter with three tissue-specific promoters(myo-2 for the pharynx, est-1 for the intestines and mec-7 for the neurons) and generated corresponding transgenic animals. Moderate defective phenotypes were observed in worms expressing SHBs Ag in the pharynx and intestines but not in worms expressing SHBs Ag in the neurons, suggesting that the secreted SHBs Ag may trigger a cross-talk signal between the digestive track and the gonad resulting in defective phenotypes. CONCLUSION Ectopic expression of three forms of HBs Ag that causes recognizable phenotypes in transgenic worms suggests that C. elegans can be used as an alternative model for studying virus-host interactions because the resulting phenotype is easily detected through microscopy. | Yi-Yin Chen Li-Wei Lee Wei-Ning Hong Szecheng J Lo | 2017 | World Journal of Virology2017，6，1： | 1 |
| 13 | 中国西沙海绵Dactylospongia elegans化学成分研究显示文摘目的对中国南海西沙群岛海绵Dactylospongia elegans的化学成分进行研究。方法利用薄层色谱、硅胶柱层析、Sephadex LH-20柱层析、MPLC、半制备HPLC等方法对化学成分进行分离纯化;通过NMR、MS等方法,并结合文献理化数据比对,鉴定化合物的结构。结果从西沙海绵Dactylospongia elegans的甲醇粗提取物中分离得到6个化合物:dactyloquinonesA-D(1~4),neodactyloquinone(5),cyclospongiaquinone-1(6)。 | 贾卓美 唐旭利 乔丹 李平林 李国强 | 2018 | 中国海洋药物2018，37，1： | 1 |
| 14 | 原位杂交检测lin-4 mRNA在Caenorhabditis elegans中的表达显示文摘lin-4是控制Caenorhabditis elegans(C.elegans)幼虫发育的异时性基因,也是一种小RNA分子(microRNA)。通过整体原位杂交检测小RNA lin-4在野生型和lin-14、lin-28突变体中的区域性表达,探讨lin-4在C.elegans发育时空控制中的作用。结果表明:lin-4 mRNA在胚胎发育的早期和中期表达,胚胎后期至L1期末没有表达,之后又持续表达,成虫中也可以检测到lin-4 mRNA的存在。在lin-14和lin-28突变体中,lin-4的表达基本与野生型一致,不受lin-14、lin-28基因突变的影响,说明lin-14和lin-28是lin-4的下游基因。 | 高雅君 杨玉荣 | 2007 | 细胞生物学杂志2007，29，2： | 1 |
| 15 | Insulin-like signaling pathway regulates integration and learning in C. elegans显示文摘Animals can sense many environment stimuli simultaneously and integrate these signals within the nervous system. However, the neural system and molecular | Yue Yang, Jiu Yaming, Xu Tao, Wu Zhengxing College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R.China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 16 | 秀丽线虫中p53/cep-1基因克隆、表达及其对寿命的影响显示文摘Caenorhabditis elegans中的cep-l基因与哺乳动物中的肿瘤抑制因子p53同源,能够引发由DNA损伤导致的细胞凋亡。cep-l基因与寿命的关系研究并不多。我们利用C.elegans的cDNA进行PCR扩增得到cep-l的片段并构建到表达载体以及RNA干扰载体上,经过IPTG诱导表达得到了约90kDa的CEP-1融合蛋白。同时对C.elegans三种虫株:野生型,daf-2(e1370),daf-16(e1038)进行RNAi(RNA-mediated interference),发现cep-l RNAi之后野生型的寿命显著延长,另外两种虫株的寿命没有显著改变,并以RT-PCR的方法检测了cep-l在这三种虫株中的表达情况。 | 苏小珊 杨玉荣 | 2010 | 中国细胞生物学学报2010，32，3： | 0 |
| 17 | In vitro biological screening of the stem of Desmodium elegans显示文摘Objective:To explore the medicinal importance of the stem of Desmodium elegans,methanolic extract,and its different solvent fractions were evaluated for brine shrimp lethality,insecticidal and phytotoxicity,antifungal,and antibacterial activities.Methods:The methanolic extract and its solvent fractions were tested for cytotoxic,phytotoxic,insecticidal,antifungal,and antibacterial effects using our previous published protocols.Results:The methanolic,DCM,ethyl acetate and n-butanol fractions exhibited insecticidal effect against Callosobruchus analis and Rhyzopertha dominie.The methanolic extract,n-hexane,DCM ethyl acetate and n-butanol showed 75,85,85,65 and 5%phytotoxicity at the tested concentration of 500μg/ml.respectively.The solvent fractions(DCM and ethyl acetate)were effective against F.solani(10%and 20%,inhibition respectively).All the tested samples were devoid of cytotoxic and antibacterial effects.Conclusions:It was concluded diat diis plant can be practiced for control of weeds and insects. | Arshad Khan Rabia Usman Ming-Liang Wang Abdur Rauf Naveed Muhammad Akhatar Aman Taha Hussein Musa Tahir | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，9： | 0 |
| 18 | Ingestion of Carbon Nanotubes and Mesoporous Silica Nanospheres by Caenorhabditis Elegans显示文摘The toxic effects to microorganism induced by nanomaterials have received considerable attentions in the past decades [1]. Herein, two diverse nanomaterials i.e. multi-walled carbon nanotubes (MWCNTs) and mesoporous silica nanospheres (MSNs) were prepared to investigate their deleterious effects on Caenorhabditis. elegans (C. elegans)[2-3]. As shown in Figure 1A, histidine functionalized MWCNTs (his-MWCNTs) were in length of ~500 nm with outer diameter ~20 nm, while fluorescein isothiocyanate dyed MSNs (FITC-MSNs) were in an average diameter of ~70 nm (Figure 1B). Microscopic images display his-MWCNTs having been ingested into intestine of C. elegans after co-incubation for 2 h, as arrowed in Figure 1C and 1E. In contrast, no MSNs were observed to be ingested after co-incubating in the same liquid medium. However, fluorescence microscopic images (Figure 1D and 1F) demonstrate that FITC-MSNs could be ingested by C. elegans after co-incubation for 24 h or longer time via seeding Kingagar plates with FITC-MSNs. | ZHANG Ye WEI Qing BI Hong QI Linxiang ZHANG Hong | 2013 | 矿物学报2013，33，S1： | 0 |
| 19 | 原位杂交检测lin-28 mRNA在Caenorhabditis elegans中的分布显示文摘利用原位杂交检测了异时性基因lin-28 mRNA在野生型和lin-4,lin-14突变体以及lin-4,lin-14双突变体中的分布,发现lin-28 mRNA在胚胎和成虫生殖腺中存在.还发现lin-4突变不改变lin-28 mRNA分布,证明了lin-28表达独立于lin-4抑制通路的存在. | 杨玉荣 郑卫玲 | 2008 | 厦门大学学报(自然科学版)2008，47，A02： | 0 |
| 20 | 原位杂交检测pal-1 mRNA在C.elegans野生型和突变体早期胚胎中的分布显示文摘Caenorhabditis elegans是发育生物学中的重要模式生物,许多基因参与C.elegans的细胞命运决定和细胞谱系发育.par基因的活性影响C.elegans胚胎前后轴的极性,其功能缺失会使胚胎的第一次分裂丧失前后轴的不对称性,导致一些母体提供的发育调控因子不能在特定的胚胎细胞中准确定位,从而改变胚胎细胞发育命运.pal-1是C.elegans早期胚胎发育中决定细胞命运的重要基因,它决定体细胞的性质,也是转录因子,调控着后续基因的表达,凡含有该基因表达的细胞发育成体细胞.调控PAL-1表达的基因有许多,本文通过原位杂交检测pal-1 mRNA在C.elegans野生型和par-5、par-6、gld-1突变体早期胚胎中的分布,来探讨par-5、par-6和gld-1基因的活性在胚胎发育早期对pal-1 mRNA的影响.实验结果表明,pal-1mRNA在野生型早期胚胎中有明显极性,而pal-1mRNA分布的不对称性在上述基因缺失的情况下被破坏,这些基因与pal-1mRNA的不对称分布直接相关. | 杨玉荣 李俊涛 | 2008 | 厦门大学学报(自然科学版)2008，47，A02： | 0 |
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