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| 1 | The relation between HLA-DQA1 genes and genetic susceptibility to duodenal ulcer in Wuhan Hans显示文摘AIM To study the genetic susceptibility of HLA-DQA1 alleles to duodenal ulcer in Wuhan Hans.METHODS Seventy patients with duodenalulcer and fifty healthy controls were examinedfor HLA-DQA1 genotypes.HLA-DQA1 typing wascarried out by digesting the locus specificpolymerase chain reaction amplified productswith alleles specific restriction enzymes(PCR-RFLP),i.e.,Apal Ⅰ,Bsaj Ⅰ,Hph Ⅰ,Fok Ⅰ,Mbo Ⅱ and Mnl Ⅰ.RESULTS The allele frequencies of DQA1 * 0301and DQA1 * 0102 in patients with duodenal ulcerwere significantly higher and lower respectivelythan those in healthy controls(0.40 vs 0.20,P = 0.003,mcorret = 0.024)and(0.05 vs 0.14,P = 0.012,but Pcorret>0.05),respectively.CONCLUSION DQA1 * 0301 is a susceptiblegene for duodenal ulcer in Wuhan Hans,andthere are immunogenetic differences in HLA-DQA1 locus between duodenal ulcer patients andhealthy controls. | Du YP Deng CS Lu DY Huang MF Guo SF Hou W | 2000 | World Journal of Gastroenterology2000，6，1： | 37 |
| 2 | Matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 expression in fibrotic rat liver显示文摘INTRODUCTIONLiver fibrosis is an excessive deposition ofextracellular matrix(ECM)resulted from bothincreased synthesis and decreased degradation.Matrix metalloproteinases(MMPs)represent agroup of neutral proteinases with variable | Liu HL Li XH Wang DY Yang SP | 2000 | World Journal of Gastroenterology2000，6，6： | 31 |
| 3 | Analysis of point mutation in site 1896 of HBV precore and its detection in the tissues and serum of HCC patients显示文摘INTRODUCTION Hepatitis B is one of the common infectious diseases,which severely impairs the health of the people in our country and has close relationship | Yuan Wang Hu Liu Qing Zhou Xu Li | 2000 | World Journal of Gastroenterology2000，6，3： | 33 |
| 4 | HCV-RNA positivity in peripheral blood mononuclear cells of patients with chronic HCV-infection: does it really mean viral replication?显示文摘AIM To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC)and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication,METHODS HCV-RNA was monitored in serumand PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-α therapy using a nested RT/ PCRtechnique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma.RESULTS In the IFN-α responding patients,HCV-RNA disappeared first from total RNApreparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNAreappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNAconcentration in serum was performed before and after transition from detectable to nondetectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMCpreparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMCfrom healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration-dependent PCR-positivity for HCV-RNA in reisolated PBMC.CONCLUSION The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMCpreparations of chronically infected patients. | Volker Meier Sabine Mihm Perdita Wietzke-Braun Guliano Ramadori | 2001 | World Journal of Gastroenterology2001，7，2： | 29 |
| 5 | Helicobacter pylori vacA genotypes and cagA status and their relationship to associated diseases显示文摘INTRODUCTIONHelicobacter pylori(H.pylori)is a major causativebacterium of chronic gastritis,peptic ulcer and mucosa-associated lymphoid tissue lymphoma in humans,andassociated with an increased risk of gastric cancer. | Hou P Tu ZX Xu GM Gong YF Ji XH Li ZS | 2000 | World Journal of Gastroenterology2000，6，4： | 27 |
| 6 | Expression of the CagA gene of H.pylori and application of its product显示文摘INTRODUCTION Helicobaeter PYlori(HP)Plays an important role inthe uPPer digestive traet diseases.It can be dividedinto two main groups(toxie and non一toxieHP)aeeording to the | Han FC Yan XJ Su CZ | 2000 | World Journal of Gastroenterology2000，6，1： | 22 |
| 7 | Relationship between expression of α-fetoprotein messenger RNA and some clinical parameters of human hepatocelular carcinoma显示文摘METHODWedetectedtheexpressionofAFPinmRNAlevelintissuesamplesfrom52patientssuferingfromHCCbyRTPCRmethod.RESULTSThepositiverat... | HE Ping, TANG Zhao You, YE Sheng Long and LIU Bin Bin | 1999 | World Journal of Gastroenterology1999，5，2： | 23 |
| 8 | Effect of 5-Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2显示文摘INTRODUCTIONHepatocellular carcinoma (HCC) is one of the mostcommon human malignancies worldwide[1,2], and isclosely associated with infection of HBV and HCVand contamination of aflatoxin B1[3-6]. Althoughthe molecular mechanisms of hepatocarcinogenesisremain poorly understood, an increasing number ofgenetic abnormalities have been recognized[7-10],for example, the p16 gene[11,12] the p53gene[13-18], the E-cadherin gene[19], and the c-mycgene[20]. | Li Hua Liu1 Wen Hua Xiao2 Wei Wen Liu3 1Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China (now working in Department of Gastroenterology, General Hospital of PLA, Lanzhou 730050, Gansu Province, China)2Department of Oncology3Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China | 2001 | World Journal of Gastroenterology2001，7，1： | 21 |
| 9 | p16 gene methylation in colorectal cancers associated with Duke's staging显示文摘AIM To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.``METHODS Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.``RESULTS pi6 methylation was detected in 42% of the tumors. Dukes staging was associated with p16methylation status, p16 methylation occurred more frequently in Dukes C and D patients (75.9%) than in Dukes A and B patients (12.1%).``CONCLUSION p16 rnethylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis. | Jing Yi~1 Zhi-Wei Wang~1 Hui Cang~1 Yu-Ying Chen~1 Ren Zhao~2 Bao-Ming Yu~2 Xue-Ming Tang~1 1 Department of Cell Biology,2 Department of Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，5： | 21 |
| 10 | Fluctuations in butyrate-producing bacteria in ulcerative colitis patients of North India显示文摘AIM: To study the interplay between butyrate concentration and butyrate-producing bacteria in fecal samples of ulcerative colitis (UC) patients vs control individuals. METHODS: Fecal samples were collected from 14 control individuals (hemorrhoid patients only) and 26 UC patients (severe: n = 12, moderate: n = 6, remission: n = 8), recruited by the gastroenterologist at the Department of Gastroenterology, All India Institute of Medical Sciences, New Delhi, India. Disease activity in UC patients was determined by clinical colitis activity index. We employed fluorescent in situ hybridization in combination with flow cytometry to enumerate the clostridium cluster population targeted by 16S rRNA gene probe. Major butyrate-producing species within this cluster were quantified to see if any change existed in control vs UC patients with different disease activity. This observed change was further validated by quantitative polymerase chain reaction. In addition to this,we carried out gas chromatography to evaluate the changes in concentration of major short chain fatty acids (SCFAs), namely acetate, n -butyrate, iso -butyrate, in the above samples. Student t test and Graph pad prism-6 were used to compare the data statistically. RESULTS: There was a significant decrease of Clostridium coccoides (control, 25.69% ± 1.62% vs severe, 9.8% ± 2.4%, P = 0.0001) and Clostridium leptum clusters (control, 13.74% ± 1.05% vs severe, 6.2% ± 1.8%, P = 0.0001) in fecal samples of UC patients. Furthermore, we demonstrated that some butyrateproducing members of the clostridial cluster, like Fecalibacterium prausnitzii (control, 11.66% ± 1.55% vs severe, 6.01% ± 1.6%, P = 0.0001) and Roseburia intestinalis (control, 14.48% ± 1.52% vs severe, 9% ± 1.83%, P = 0.02) were differentially present in patients with different disease activity. In addition, we also demonstrated decreased concentrations of fecal SCFAs, especially of n -butyrate (control, 24.32 ± 1.86 mmol/μL vs severe, 12.74 ± 2.75 mmol/μL, P = 0.003), iso -butyrate (control, 1.70 ± 0.41 mmol/μL vs severe, 0.68 ± 0.24 mmol/μL, P = 0.0441) and acetate (control, 39.51 ± 1.76 mmol/μL vs severe, 32.12 ± 2.95 mmol/ μL,P = 0.047), in the fecal samples of UC patients. The observed decrease of predominant butyrate producers of clostridial clusters correlated with the reduced SCFA levels in active UC patients. This was further confirmed by the restoration in the population of some butyrate producers with simultaneous increase in the level of SCFA in remission samples. CONCLUSION: Our observations indicate that decreases in members of the clostridial cluster resulting in reduced butyrate levels contribute to the etiology of UC. | Reena Kumari Vineet Ahuja Jaishree Paul | 2013 | World Journal of Gastroenterology2013，19，22： | 21 |
| 11 | Study on the expression of matrix metalloproteinase-2 mRNA in human gastric cancer显示文摘 | Ji F Wang WL Yang ZL Li YM Huang HD Chen WD | 1999 | World Journal of Gastroenterology1999，5，5： | 19 |
| 12 | Detection of hepatitis C virus NS5 protein and genome in Chinese carcinoma of the extrahepatic bile duct and its significance显示文摘AIM To investigate the hepatitis C virus(HCV)infection in the tissues of carcinoma ofextrahepatic bile duct and study theircorrelation.METHODS HCV NS5 protein and HCV RNA weredetected by labeled streptavidin biotin(LSAB)method and in situ reverse transcriptionpolymerase chain reaction(IS-RT-PCR)insections of 51 cases of carcinoma ofextrahepatic bile duct and 34 cases of controlgroup(without malignant biliary disease).RESULTS In 51 cases of carcinoma ofextrahepatic bile duct,HCV NS5 protein wasdetected in 14(27.5%),which was clearlystained in the cytoplasm of cancer cell but not inthe nucleus or cell membrane.HCV RNA wasdetected in 18(35.4%),which was located inthe nucleus of cancer cell in 12 cases and in thecytoplasm in 6 cases.HCV NS5 protein and RNAcoexistence was found in 2 cases.In 34 cases ofcontrol group,HCV RNA was detected in 2(5.9%).HCV NS5 protein and RNA positive cellswere found either scattered or in clusters.CONCLUSION The prevalence of hepatitis C viral infection in the tissues of carcinoma ofextrahepatic bile duct was significantly higherthan in control group(X^2=9.808,P=0.002).The findings suggest a correlation between HCVinfection and carcinoma of extrahepatic bileduct,which is different from the traditionalviewpoint.HCV infection might be involved inthe development of carcinoma of extrahepaticbile duct. | Ming Yi Chen Zhi Qiang Huang Le Zhen Chen Ya Bing Gao Rui Yun Peng De Wen Wang | 2000 | World Journal of Gastroenterology2000，6，6： | 19 |
| 13 | The cloning of 3'-truncated preS/S gene from HBV genomic DNA and its expression in transgenic mice显示文摘INTRODUCTIONHepatitis B virus (HBV) is regarded as one of themain etiologic factors involved in the developmentof human hepatocellular carcinoma (HCC).The open reading frame (orf)of X gene of HBVencoded a transactivating factor is the evidence thatstrongly supported the notion that the X gene ofHBV DNA integrated in HCC genomic DNA couldcontribute to the carcinogenesis of liver cells byactivation of some related cellular genes | Yi Ping Hu~1 Yu Cheng Yao~1 Jian Xiu Li~1 Xin Min Wang~1 Hong Li~2 Zhong Hua Wang~1 Zhang Heng Lei~3 1 Department of Cell Biology,Second Military Medical University,Shanghai 200433,China 2 Department of Biology,Department of Basic Medicine,West-China University of Medical Sciences,Chengdu 610041,China 3 Department of Biology,North Sichuan Medical College,Nanchong 637007,China | 2000 | World Journal of Gastroenterology2000，6，5： | 18 |
| 14 | Codon 249 mutations of p53 gene in development of hepatocellular carcinoma显示文摘Codon249mutationsofp53geneindevelopmentofhepatocelularcarcinomaPENGXiaoMou,PENGWenWeiandYAOJiLuSubjectheadingsliverneopla... | Peng, XM Peng, WW Yao, JL | 1998 | World Journal of Gastroenterology1998，4，2： | 18 |
| 15 | 实时荧光定量PCR定量方法研究进展显示文摘实时荧光定量PCR以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为了分子生物学研究中的重要工具,综述了实时荧光定量PCR技术及其定量方法的研究进展,并展望了其应用前景。 | 廉红霞 高腾云 傅彤 孙宇 李改英 | 2010 | 江西农业学报2010，22，10： | 17 |
| 16 | Infrequent p53 gene mutation and expression of the cardia adenocarcinomas from a high-incidence area of Southwest China显示文摘INTRODUCTIONAdenocarcinomas of the cardia are the lesionsarising from the proximal stomach or within 3 cm ofthe gastroesophageal junction.These cancerstended to be advanced at the time of presentation,usually with poor prognosis.In recent decade,the incidence of adenocarcinoma of gastric eardiaand esophagus are increasing steadily,while therehas been a decrease in the proportion of the cancersarising from the distal stomach.The | Naoko lida Hideaki Oda Shigetoshi Aiso Takatoshi Ishikawa | 2000 | World Journal of Gastroenterology2000，6，5： | 17 |
| 17 | Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients显示文摘AIM: To investigate Fusobacterium nucleatum(F. nucleatum) abundance in colorectal cancer(CRC) tissues and its association with CRC invasiveness in Chinese patients.METHODS: The resected cancer and adjacent normal tissues(10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction(FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization(FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results.RESULTS: The median abundance of F. nucleatum in CRC tissues [0.242(0.178-0.276)] was significantly higher than that in normal controls [0.050(0.023-0.067)](P < 0.001). F. nucleatum was over-represented in 88/101(87.1%) CRC samples. The abundance of F. nucleatum determined by 2^(-ΔCT) was significantly greater in tumor samples [0.242(0.178, 0.276)] than in normal controls [0.050(0.023, 0.067)](P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88(59.1%)] than in the under-abundance group [0/13(0%)](P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed(P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues(median number 6, 25^(th) 3, 75^(th) 10 vs 2, 25^(th) 1, 75^(th) 5)(P < 0.01).CONCLUSION: F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis. | Yu-Yuan Li Quan-Xing Ge Jie Cao Yong-Jian Zhou Yan-Lei Du Bo Shen Yu-Jui Yvonne Wan Yu-Qiang Nie | 2016 | World Journal of Gastroenterology2016，22，11： | 17 |
| 18 | Follow-up study of hepatitis C virus infection in uremic patients on maintenance hemodialysis for 30 months显示文摘INTRODUCTIONA high prevalence of antibodies to hepatitis C virus(HCV)(range from 3.3%-80%)has beenreported in hemodialysis(HD)patients,andworrisome as it often becomes chronic and induceschronic liver disease,therefore thenephrologists face a major challenge of how toprevent it.The main route of HCV transmission | Nian Song Wang Lu Tan Liao Yan Juan Zhu Wei Pan Fang Fang | 2000 | World Journal of Gastroenterology2000，6，6： | 17 |
| 19 | Naturally occurring hepatitis B virus reverse transcriptase mutations related to potential antiviral drug resistance and liver disease progression显示文摘The annual number of deaths caused by hepatitis B virus(HBV)-related disease, including cirrhosis and hepatocellular carcinoma(HCC), is estimated as 887000. The reported prevalence of HBV reverse transcriptase(RT) mutation prior to treatment is varied and the impact of preexisting mutations on the treatment of na?ve patients remains controversial, and primarily depends on geographic factors, HBV genotypes, HBe Ag serostatus, HBV viral loads, disease progression, intergenotypic recombination and co-infection with HIV. Different sensitivity of detection methodology used could also affect their prevalence results. Several genotype-dependent HBV RT positions that can affect the emergence of drug resistance have also been reported. Eight mutations in RT(rtL80I, rtD134N, rtN139K/T/H, rtY141F, rtM204I/V, rtF221Y, rtI224V, and rtM309K) are significantly associated with HCC progression. HBe Ag-negative status, low viral load, and genotype C infection are significantly related to a higher frequency and prevalence of preexisting RT mutations. Preexisting mutations are most frequently found in the A-B interdomain of RT which overlaps with the HBs Ag 'a' determinant region, mutations of which can lead to simultaneous viral immune escape. In conclusion, the presence of baseline RT mutations can affect drug treatment outcomes and disease progression in HBVinfected populations via modulation of viral fitness and host-immune responses. | Yu-Min Choi So-Young Lee Bum-Joon Kim | 2018 | World Journal of Gastroenterology2018，24，16： | 17 |
| 20 | Sequencing of PCR amplified HBV DNA pre-c and c regions in 2.2.15 cells and antiviral action by targeted antisense oligonucleotide directed against sequence显示文摘INTRODUCTIONManystudieshaveshownthatantisenseoligonucleotides(ASONs)canefficientlyinhibitHBVDNAreplicationandexpresioninvitro... | ZHOUG Sen 1, WEN Shou Ming 2, ZHANG Ding Feng 3, WANG Quan Li 4, WANG Seng Qi 4 and REN Hong 3 | 1998 | World Journal of Gastroenterology1998，4，5： | 16 |
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