|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 肠道菌群与肠道屏障互作在炎症性肠病中的作用研究进展显示文摘炎症性肠病(inflammatory bowel disease,IBD)是一种慢性非特异性肠道炎症性疾病,其发病机制复杂。IBD患者肠道菌群的组成与结构发生明显变化即肠道菌群失调。肠道屏障功能对维持人体肠道功能稳态发挥着重要调节作用。研究表明,在IBD中存在肠道屏障功能受损、肠道通透性增加。肠道菌群失调导致肠道屏障功能受损,从而破坏人体正常的肠道稳态功能,加重IBD的进展。本文将对肠道菌群与肠道屏障功能之间的互作在IBD发生发展及治疗中的应用进行阐述。这将为IBD的防治提供理论基础和新思路。 | 李瑶 黄金莉 黄娟 李华军 | 2021 | 胃肠病学和肝病学杂志2021，30，1： | 21 |
| 2 | Exploring gut microbes in human health and disease:Pushing the envelope显示文摘Humans have coevolved with their microbes over thousands of years,but this relationship,is now being dramatically affected by shifts in the collective human microbiome resulting from changes in the environment and societal norms.Resulting perturbations of intestinal host-microbe interactions can lead to miscues and altered host responses that increase the risk of pathogenic processes and promote“western”disorders such as inflammatory bowel diseases,cancers,obesity,diabetes,autism,and asthma.Given the current challenges and limitations in gene therapy,approaches that can reshape the gut microbiome represent a reasonable strategy for restoring the balance between host and microbes.In this review and commentary,we highlight recent progress in our understanding of the intestinal microbiome in the context of health and diseases,focusing on mechanistic concepts that underlie the complex relationships between host and microbes.Despite these gains,many challenges lie ahead that make it difficult to close the gap between the basic sciences and clinical application.We will discuss the potential therapeutic strategies that can be used to manipulate the gut microbiota,recognizing that the promise of pharmabiotics(“bugs to drugs”)is unlikely to be completely fulfilled without a greater understanding of enteric microbiota and its impact on mammalian physiology.By leveraging the knowledge gained through these studies,we will be prepared to enter the era of personalized medicine where clinical inventions can be custom-tailored to individual patients to achieve better outcomes. | Jun Sun Eugene B.Chang | 2014 | Genes & Diseases2014，1，2： | 17 |
| 3 | 肠道菌群在功能性便秘中的作用显示文摘功能性便秘非常常见,但其发病机制尚不明了。越来越多的证据显示,功能性便秘与肠道微生态失调有关,而肠道微生态与肠道转运之间很可能存在相互影响。本文对功能性便秘发生过程中肠道微生态所发挥作用的证据进行了总结。肠道微生态通过胆酸、短链脂肪酸、5-羟色胺和甲烷等相关微生物代谢活动调控结肠动力、结肠黏膜分泌和吸收,进而促使功能性便秘的发生。作为肠道微生态影响功能性便秘的证据支持,用益生菌、益生元、合生元和中药治疗功能性便秘时,常可观察到患者肠道微生态在菌种组成和功能上的改变。深入研究功能性便秘的病理发生,阐明微生态制剂治疗功能性便秘的药理机制,将为功能性便秘的新型治疗方法提供理论基础。 | Shengsheng Zhang Ruixin Wang Danyan Li Luqing Zhao Lixin Zhu | 2021 | Gastroenterology Report2021，9，5： | 13 |
| 4 | Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs显示文摘Background: Pork produced by outdoor-reared pigs raised mostly on alfalfa pastures attracts increasing population of consumer from most of the world. In China, pigs were raised with alfalfa-containing diets to seek for good quality pork.However, the influence of dietary alfalfa involving high level of insoluble dietary fiber(IDF) on pig intestinal luminal microbiota composition remains unclear. The objective of this study was to investigate the effects of alfalfa on luminal microbiota and short chain fatty acids(SCFA) production, and gene expressions involved in SCFA sensing, transporting and absorbing in pig caecal mucosa.Results: Twenty-four growing pigs were randomly allotted to four diets containing 0%, 5%, 10% and 15% alfalfa meal for a 28-d experiment. Ingestion of alfalfa meal-contained diets significantly increased the ratio of body weight gain to feed consumption. Illumina MiS eq sequencing of the V3 region of the 16 S r RNA genes showed that alfalfa-containing diet significantly decreased the relative abundance of genera Turicibacter, Acidiphilium, Paracoccus, Propionibacterium,Corynebacterium, Pseudomonas, Acinetobacter, and Staphylococcus, and increased the relative abundance of genera Lachnospira, Marvinbryantia, and Desulfovibrio in the caecal digesta. Butyrate concentration was significantly increased in the hindgut by the supplementation of alfalfa meal in diets. The m RNA gene expressions of FFAR3, SMCT1, MCT1,PYY, and GCG were significantly increased in the caecal mucosa of pigs fed alfalfa meal.Conclusions: Our results suggested that alfalfa-containing diet has exerted significant impacts on caecal microbiota composition, butyrate concentration and significantly upregulated m RNA expression of host caecal mucosal genes involved in SCFA sensing and absorption as well as regulation of satiety. | Jiawei Wang Chunfu Qin Ting He Kai Qiu Wenjuan Sun Xin Zhang Ning Jiao Weiyun Zhu Jingdong Yin | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 12 |
| 5 | 香蕉粉干预对糖尿病大鼠肠道菌群调节作用研究显示文摘观察香蕉粉干预对糖尿病大鼠肠道菌群的影响。将SD大鼠分为:正常对照组(NCG)、糖尿病对照组(DCG)、低剂量组(LDG)、中剂量组(MDG)和高剂量组(HDG),干预4周后检测5种常见肠道菌群的分布。结果表明:干预后高剂量组(263.50±8.71g)和中剂量组(258.25±11.89 g)的体重显著低于糖尿病对照组(278.75±5.06 g);各干预组的排便量(182.57±12.80 g、188.00±17.09g、163.00±8.75 g)显著高于糖尿病对照组(146.57±20.44 g);香蕉粉可显著增加双歧杆菌、拟杆菌、乳酸杆菌的菌落数,显著减少肠球菌菌落数,各干预组的B/E值(1.17±0.15、1.24±0.08、1.21±0.11)显著高于糖尿病对照组(1.13±0.07);各干预组的短链脂肪酸含量(188.50±46.90 mmol/L、118.60±32.41 mmol/L、61.42±10.80mmol/L)显著高于糖尿病对照组(32.46±8.34 mmol/L),同时pH值显著降低。香蕉粉有控制糖尿病大鼠体重增加和促进排便的作用,明显改善肠道菌群结构,并增加其酵解产物,降低肠道pH值,从而发挥其对机体的健康作用。 | 白永亮 彭真福 陈庆发 周洁静 杨公明 | 2013 | 现代食品科技2013，29，9： | 11 |
| 6 | Supplementation of milk formula with galacto-oligosaccharides improves intestinal micro-flora and fermentation in term infants显示文摘Background Oligosaccharides in human milk may protect infants by improving the intestinal micro-flora and fermentation. This study was to investigate effects of infant formula milk consisting of galacto-oligosaccharide (GOS) on intestinal microbial populations and the fermentation characteristics in term infants in comparison with that of human milk. Methods The test formula (Frisolac H,Friesland,Netherland) was supplemented with GOS at a concentration of 0.24 g/dl. Human milk and another formula without oligosaccharides (Frisolac H,Friesland,Netherland) were used as positive and negative control respectively. Growth,stool characteristics,and side effects of the recruited infants were recorded after 3 and 6 months’ follow-up,and the fecal species were collected for the analysis of intestinal micro-flora,short chain fatty acid (SCFA) and pH.Results At the end of 3- and 6-month feeding period,intestinal Bifidobacteria and Lactobacilli were significantly increased in infants fed with GOS supplemented formula and human milk when compared with infants fed with negative control formula; however,there was no statistically significant difference between GOS supplemented formula and human milk groups. Stool characteristics were influenced by the supplement and main fecal SCFA (acetic),and stool frequency were significantly increased in infants fed with GOS supplemented formula and human milk,while the fecal pH was significantly decreased as compared with that of negative control ( P <0.05). Supplementation had no influence on incidence of side effects (including crying,regurgitation and vomiting). Conclusions Supplementing infant formula with GOS at a concentration of 0.24 g/dl stimulates the growth of Bifidobacteria and Lactobacilli in the intestine and stool characteristics are similar to in term infants fed with human milk. | 贲晓明 周晓玉 赵卫华 喻文亮 潘伟 张伟利 吴圣楣 Christien M. Van Beusekom Anne Schaafsma | 2004 | Chinese Medical Journal2004，，6： | 10 |
| 7 | 低聚合度魔芋甘露寡糖对正常小鼠肠道及微生物菌群的影响显示文摘研究以有机溶剂沉淀法制备的低聚合度魔芋甘露寡糖(LMOS)对正常小鼠肠道形态、肠道菌群的影响。取ICR小鼠50只,随机分为4组实验组和1组对照组,分别用不同剂量1,2,4,8,8g/(kg·d)的LMOS溶液和等体积的生理盐水灌胃,连续30 d后,观察比较不同LMOS添加量对小鼠肠道形态、盲肠内短链脂肪酸(SCFA)及微生物菌群的影响。实验结果表明,与对照组相比,高剂量的LMOS能显著增加小鼠盲肠内SCFA的含量,并能使肠绒毛高度增加。变性梯度凝胶电泳(DGGE)指纹图谱结果分析表明,与对照组相比,喂食高剂量LMOS能使小鼠肠道中乳杆菌等有益菌含量增加。得出LMOS能够通过促进SCFA的产生、促进乳杆菌的增殖,调节小鼠肠道健康,是一类具有开发前景的益生元。 | 万嘉佳 蒋敏 李恒 罗春勤 史劲松 | 2015 | 食品与发酵工业2015，41，9： | 6 |
| 8 | 气相色谱法检测粪便中短链脂肪酸的前处理方法显示文摘目的:探索一种适合利用气相色谱法检测粪便中短链脂肪酸(short chain fatty acid,SCFA)的前处理方法。方法:改变粪便样品前处理中所涉及的稀释液种类、稀释液体积、离心转速等实验条件,利用气相色谱法检测不同前处理的粪便样品中SCFA的种类和含量,通过加标回收实验对选择的前处理方法进行验证。结果:选择8 m L酸稀释液处理样品,15 000 r/min离心20 min作为样品的前处理方法,色谱图显示各种SCFA峰形较好,达到基线分离。加标回收率在83.75%~94.95%,符合国家标准。结论:实验中所选择的前处理方法切实可行,适用于粪便中SCFA分析。 | 张梦洁 袁甜 史雪敏 贾海莲 高磊 侯瑞丽 包艳 | 2017 | 包头医学院学报2017，33，11： | 5 |
| 9 | MCT1在犊牛消化道的分布以及SCFA对MCT1表达的影响显示文摘短链脂肪酸(SCFAs)是由纤维素性饮食在瘤胃内发酵产生的乙酸、丙酸、丁酸以及戊酸等组成的混合物。SCFA对维持反刍动物的能量平衡至关重要,目前对SCFA的吸收机制和影响因素至今尚不清楚,本试验主要研究一元羧酸转运蛋白(monocarboxylate/proton cotransporter isoform 1,MCT1)在犊牛消化道内的分布及SCFA对MCT1表达的影响。运用免疫印迹Western blot和实时荧光定量PCR检测MCT1在犊牛消化道内的分布和表达;在体外试验中,体外添加一定比例的SCFA(乙酸、丙酸和丁酸的混合物)处理犊牛原代瘤胃上皮细胞,检测SCFA对MCT1表达水平的影响。结果显示:MCT1在整个消化道内均有分布,并且在瘤胃内的表达水平显著高于其他部位(P<0.01);体外处理的瘤胃上皮细胞,正常组MCT1的表达水平显著高于(P<0.05)SARA组。结果表明:正常水平的SCFA能够促进MCT1的表达和转运能力,而过量的SCFA抑制MCT1的表达和转运能力。 | 王亚洲 孙国权 赵晨旭 魏园 崔媛旭 苑学 王哲 李心慰 刘国文 | 2017 | 中国兽医学报2017，37，3： | 5 |
| 10 | 营养支持中脂肪制剂的发展显示文摘1915年美国的Murlin和Riche即开始给动物静脉输注脂肪乳剂;1920年Ya-makawa将脂肪乳剂用于人体;1935年Em-metHolt首次应用棉子油乳剂输注。然而,这些制剂均未发展到临床应用。继后,Geyer和Stare报道了用卵磷脂和... | 张利华 黎介寿 | 1998 | 肠外与肠内营养1998，5，1： | 4 |
| 11 | 藏绵羊暖、冷季瘤胃微生物菌群密度与其短链脂肪酸浓度互作分析显示文摘针对藏绵羊暖、冷季营养供给严重不均衡的生产实际,本试验旨在解析藏绵羊应对冷季营养严重匮乏的适应机制。试验选择1周岁(±1月龄)健康的放牧藏绵羊母羊12只,采用气相色谱仪和荧光定量PCR仪分别测定藏绵羊暖(7月)、冷(12月)季瘤胃短链脂肪酸(SCFA)浓度和微生物菌群密度,分析藏绵羊暖、冷季瘤胃SCFA浓度与微生物菌群密度间的互作关系。结果表明:藏绵羊冷季瘤胃中乙酸与丙酸浓度及比值(A/P)显著高于暖季(P<0.05),其他SCFA浓度在暖、冷季间差异不显著(P>0.05);各种SCFA所占比例分析发现,乙酸比例在冷季显著高于暖季(P<0.05),丁酸比例在暖季显著高于冷季(P<0.05),异丁酸比例在暖、冷季间差异不显著(P>0.05)。随着季节的变化,藏绵羊瘤胃微生物中黄色瘤胃球菌(Ruminococcus flavefaciens)、白色瘤胃球菌(Ruminococcus albus)、产琥珀酸丝状杆菌(Fibrobacter succinogenes)、溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)、嗜淀粉瘤胃杆菌(Ruminobacter amylophilus)与产甲烷菌(Methanogenic bacteria)的相对密度在暖季显著高于冷季(P<0.05)。相关性分析表明,乙酸浓度与黄色瘤胃球菌相对密度,丙酸浓度与白色瘤胃球菌相对密度呈极显著正相关(P<0.01),乙酸浓度与白色瘤胃球菌、嗜淀粉瘤胃杆菌、溶纤维丁酸弧菌、产甲烷菌相对密度,丙酸浓度与产琥珀酸丝状杆菌相对密度呈显著负相关(P<0.05),丁酸浓度与6种微生物菌种相对密度无显著相关性(P>0.05)。由此可见,藏绵羊瘤胃微生物菌群密度与SCFA浓度间的互作存在明显差异,这种互作机制可能在藏绵羊应对暖、冷季的适应性方面发挥着重要作用,研究结果将为藏绵羊产业发展及种质资源保护利用提供参考。 | 史浩 沙玉柱 魏红 吕卫兵 文禹梁 刘秀 罗玉柱 王继卿 李少斌 胡江 | 2021 | 动物营养学报2021，33，2： | 3 |
| 12 | 4种粮食体外发酵特性研究显示文摘采用体外发酵技术比较4种粮食发酵特性,评价高粱、黄豆、早籼米(糙米和精制白米)发酵性能。将4种食物制成冻干粉加入培养基,接种人体新鲜粪便进行体外发酵,定点记录发酵液产气量、测定pH、氨和SCFA含量。黄豆组产气量最大,空白组产氨量最多且pH最高,高粱组pH最低,产氨量最少。高粱组总短链脂肪酸含量最高,空白组最低。4种粮食体外发酵有助于增加SCFA,高粱优于其他三种。 | 罗晓燕 蔡力创 阮征 黄小流 刘世强 欧阳崇学 | 2013 | 食品研究与开发2013，34，16： | 3 |
| 13 | Eggshell and environmental bacteria contribute to the intestinal microbiota of growing chickens显示文摘Background: The initial intestinal microbiota acquired from different sources has profound impacts on animal health and productivity. In modern poultry production practices, the source(s) of the establishing microbes and their overall contribution during development of gastrointestinal tract communities are still unclear. Using fertilized eggs from two independent sources, we assessed the impact of eggshell-and environmental-associated microbial communities on the successional processes and bacterial community structure throughout the intestinal tract of chickens for up to 6 weeks post-hatch.Results: Culturing and sequencing techniques identified a viable, highly diverse population of anaerobic bacteria on the eggshell. The jejunal, ileal, and cecal microbial communities for the egg-only, environment-only, and conventionally raised birds generally displayed similar successional patterns characterized by increasing community richness and evenness over time, with strains of Enterococcus, Romboutsia, and unclassified Lachnospiraceae abundant for all three input groups in both trials. Bacterial community structures differed significantly based on trial and microbiota input with the exception of the egg-exposed and conventional birds in the jejunum at week 1 and the ileum at week 6. Cecal community structures were different based on trial and microbiota input source, and cecal short-chain fatty acid profiles at week 6 highlighted functional differences as well.Conclusion: We identified distinct intestinal microbial communities and differing cecal short-chain fatty acid profiles between birds exposed to the microbiota associated with either the eggshell or environment, and those of conventionally hatched birds. Our data suggest the eggshell plays an appreciable role in the development of the chicken intestinal microbiota, especially in the jejunum and ileum where the community structure of the eggshellonly birds was similar to the structure of conventionally hatched birds. Our data identify a complex interplay between the eggshell and environmental microbiota during establishment and succession within the chicken gut.Further studies should explore the ability of eggshell-and environment-derived microbes to shape the dynamics of succession and how these communities can be targeted through interventions to promote gut health and mitigate food-borne pathogen colonization in poultry. | Joel J.Maki Elizabeth A.Bobeck Matthew J.Sylte Torey Looft | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 2 |
| 14 | Effects of drinking water supplementation with Lactobacillus reuteri,and a mixture of reuterin and microcin J25 on the growth performance,caecal microbiota and selected metabolites of broiler chickens显示文摘Background:Since the overuse of antibiotics in animal production has led to a selection of antibiotic-resistant pathogens that affect humans and animals as well.Scientists are therefore searching for novel natural alternatives to antibiotics.In this study Lactobacillus reuteri and a combination of reuterin and microcin J25(RJ)were evaluated as promoters of growth and modulators of the cecal microbiota and metabolite profiles in broiler chickens.Oneday-old Cobb 500 male broilers were distributed to 8 treatments:negative control(without antibiotic),positive control(bacitracin),three concentrations of RJ and three doses of L.reuteri plus glycerol.The birds(2176,34 per pen,8 pens per treatment)were reared for 35 d.Results:The body weight of the bacitracin and 5 mmol/L reuterin combined with 0.08μmol/L microcin J25(10RJ)treatment group was significantly higher than that of the negative control group(P<0.05).L.reuteri had no significant effect on broiler growth.MiSeq high-throughput sequencing of 16S rRNA showed clustering of cecal microbial operational taxonomic unit diversity according to treatment.The influence of bacitracin and 10RJ on bacterial community overall structure was similar.They promoted Ruminococcaceae,Lachnospiraceae and Lactobacillaceae,increased the relative abundance of Faecalibacterium and decreased the abundance of Bacteroides and Alistipes,while the negative control condition favored Bacteroidaceae and Rikenellaceae.Furthermore,10RJ increased the concentration of short-chain fatty acid in the cecum and changed the metabolome overall.Conclusions:These overall suggest that 10RJ can promote a host-friendly gut environment by changing the cecal microbiome and metabolome.This combination of natural antimicrobial agents in the drinking water had a positive effect on broiler growth and may be suitable as an alternative to antibiotic growth promoters. | Liya Zhang Laila Ben Said Nadège Hervé Séverine Zirah Moussa Sory Diarra Ismail Fliss | 2022 | Journal of Animal Science and Biotechnology2022，13，4： | 1 |
| 15 | 污泥厌氧资源化利用积累SCFA的新策略及作用机制显示文摘为了探究磁铁矿对剩余污泥厌氧发酵生产短链脂肪酸(SCFA)的影响,以剩余污泥为研究对象,建立序批式厌氧反应器,通过比较pH,溶解性化学需氧量(SCOD),SCFA及乙酸钠的变化探究了磁铁矿纳米材料(Fe3O4NPs)对污泥厌氧发酵的影响。实验结果表明Fe3O4纳米材料的存在能够增加SCOD和SCFA含量,当Fe3O4NPs由0 mg/L增加至300 mg/L时,SCOD和SCFA的最大质量浓度分别由3256、1524 mg/L增加至4015、3460 mg/L。进一步研究发现适量Fe3O4NPs投加有助于乙酸钠的降解,而过量Fe3O4NPs的投加却抑制乙酸钠的降解。适量Fe3O4NPs的投加为厌氧微生物提供了微量元素,强化了微生物的活性。揭示Fe3O4NPs对污泥厌氧消化产酸的影响行为,为推动污泥厌氧消化技术进步提供理论依据和技术支撑。 | 王一冰 王德英 | 2021 | 水处理技术2021，47，1： | 1 |
| 16 | 逐步式pH强化污泥厌氧发酵生产短链脂肪酸显示文摘介绍了一种利用逐步式pH强化污泥厌氧发酵生产短链脂肪酸(SCFA)的新方法。结果表明:在半连续流厌氧反应器中,pH由7升高至8时,提高了气相中甲烷和CO_2的分压,进一步升高至9和10,却显著降低了甲烷分压。当pH为9时,SCFA的最大产量为358.9 mg/g。机理研究发现:当pH由7升高至10时,SCOD、溶解性蛋白质、溶解性多糖的含量均呈现上升趋势,SCOD的含量由485 mg/L升高至685 mg/L,溶解性蛋白质的含量由235 mg/L升高至386 mg/L,而溶解性多糖的含量则由51 mg/L升高至92 mg/L。挥发性悬浮固体(VSS)减量随pH升高呈现上升趋势。微生物种群结构分析表明:pH的升高降低了Euryarchaeota的相对丰度,而升高了Firmicutes的相对丰度。 | 杨平平 | 2018 | 环境工程2018，36，12： | 1 |
| 17 | 短链脂肪酸作用下伤寒沙门菌诱导巨噬细胞凋亡机制的探讨显示文摘目的了解短链脂肪酸(SCFA)作用伤寒沙门菌诱导巨噬细胞凋亡机制。方法将SCFA作用伤寒沙门菌感染巨噬细胞8 h后,检测TNF-α、caspase3、caspase8、caspase9及NO的产生量,同时检测加入caspase3、caspase8、caspase9抑制剂和TNF-α抗体后的细胞凋亡率。结果作用8 h后caspase3、caspase8及NO、TNF-α的产生量均高于对照组(P<0.01)。caspase3、caspase8抑制剂和TNF-α抗体均能不同程度抑制SCFA作用伤寒沙门菌诱导的巨噬细胞凋亡(P<0.01)。结论 SCFA作用伤寒沙门菌诱导巨噬细胞凋亡可以通过NO及TNF-α介导,caspase3和caspase8参与的外源性凋亡途迳。 | 刘婷婷 马丽娜 李凤云 刘勇 樊蓉 | 2010 | 中国微生态学杂志2010，22，10： | 0 |
| 18 | Main Melody Configuration and Chord Algorithm for Relaxing Music Generation显示文摘This study applies the diatonic chord in music theory,utilization rate,and the close relationship between the main chord system,the dominant chord system,and the subordinate chord system.From the perspective of music theory,the computer can automatically and quickly analyze the music,and establish a set of algorithms for configuring the chord accompaniment for the main melody,called the symmetrical circle offifths algorithm,SCFA(Symmetrical Circle of Fifths Algorithm).SCFA can immediately confirm the key,perform harmony analysis,configure chord accompaniment for the main melody,and effectively and correctly complete any given melody or interval.It can also quickly analyze and correctly configure the chord accompaniment for any MIDI(Musical Instrument Digital Interface)music,enriching the musicality of the music.It can also allow scorers or computer music creators to quickly deconstruct the harmony configuration of the melody.Through the measurement of bio-feedback sensor HRV(Heart Rate Variability),it can achieve a relaxing music healing effect. | Chih-Fang Huang Ai-Hsien Fan Jin-Huang Huang Hsing-Cheng Huang | 2023 | Intelligent Automation & Soft Computing2023，，1： | 0 |
| 19 | The association between the metabolites of intestinal flora and diabetes mellitus显示文摘Objective To contrast and analyze the difference of metabolites of intestinal flora between patients with type 2diabetes and normal blood glucose human in Xinjiang Uygur and Kazak.Methods Uygur fecal samples and Kazak fecal samples were collected,and short-chain fatty acids(SCFA)were extracted by water from feces,then the content of SCFA in fecal samples was analyzed by gas-chromatography mass-spectrometry(GC-MS).Results Both Uygurs and Kazaks,in their feces,the content of SCFA in the feces showed significantly decreased in patients with type 2diabetes,and a significant negative linear correlation was shown between the fasting plasma glucose and the concentration of SCFA in the fecal samples.But,there was no statistical differences between uygurs and kazaks,in not only normal blood glucose group,but also type 2 diabetes group.Conclusion The feces of patients with type 2diabetes has an decreased concentration of SCFA.Between Uygurs and Kazaks,the metabolic changes of intestinal flora was similar in patients with type 2diabetes. | WANG Ye WANG Chang-hui YU Xin-lan LI Ge LIU Jing-bao GAO Bing MAO Xin-min LI Lin-lin | 2010 | 新疆医科大学学报2010，33，9： | 0 |
| 20 | 梯度乙醇沉淀法提取杏鲍菇多糖及其抗氧化和免疫刺激活性研究显示文摘食用菌多糖具有多种生理活性,这些活性与其组成及结构等有直接的关系。杏鲍菇在我国栽培面积大,对其深度开发具有重要的经济价值。本文的目的是提取杏鲍菇多糖,并通过不同浓度的乙醇沉淀获得各级醇沉多糖,并评价其体内免疫调节活性。在温和条件下,从杏鲍菇子实体中提取多糖,并通过20%、.40%、60%80%梯度乙醇沉淀法或传统的80%乙醇沉淀获得不同的多糖组分(分别命名为PP20、PP40、PP60、PP80和TPP),通过高效液相色谱(HPLC)进行定性和定量分析;然后将多糖给小鼠灌胃。评估了杏鲍菇多糖的抗氧化能力及其对小鼠免疫活性和肠道短链脂肪酸(SCFA)的调节作用。结果表明,PP60和TPP中含有岩藻糖成分,而在PP80和TPP中含有核糖。PP60和PP80处理小鼠体内中超氧阴离子的清除率显著高于PP40和正常对照组(p<0.01)。PP80组的血清IL-6浓度明显低于正常对照组(p<0.01)。PP60组的血清TNF-α浓度降至82.13 pg·mL^-1,明显低于PP40组和空白对照组(p<0.01)。所有经过多糖处理的组在粪便中均显示出较高的短链脂肪酸(SCFA)含量。 | 孙庆申 赵越龙 王鑫 刘欣洋 韩晓云 李梦洋 | 2020 | 黑龙江大学自然科学学报2020，37，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员