Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs

查看全文 作　　者：Jiawei [1,2,3]Wang;Chunfu [1]Qin;Ting [1]He;Kai [1]Qiu;Wenjuan [1]Sun;Xin [1]Zhang;Ning [1]Jiao;Weiyun [2]Zhu;Jingdong [1]Yin

机构地区：[1]State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University;[2]Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University;[3]College of Biotechnology and Food Science, Hebei Normal University for Nationalities

出　　处：《Journal of Animal Science and Biotechnology》索引2018年第9卷第2期,共9页

基　　金：financially supported by the National Key Basic Research Program of China(2012CB124702,2013CB127302);National Natural Science Foundation of China(31272452);the National Key Technology R&D Program of China(2011BAD26B01) 摘　　要：Background: Pork produced by outdoor-reared pigs raised mostly on alfalfa pastures attracts increasing population of consumer from most of the world. In China, pigs were raised with alfalfa-containing diets to seek for good quality pork.However, the influence of dietary alfalfa involving high level of insoluble dietary fiber(IDF) on pig intestinal luminal microbiota composition remains unclear. The objective of this study was to investigate the effects of alfalfa on luminal microbiota and short chain fatty acids(SCFA) production, and gene expressions involved in SCFA sensing, transporting and absorbing in pig caecal mucosa.Results: Twenty-four growing pigs were randomly allotted to four diets containing 0%, 5%, 10% and 15% alfalfa meal for a 28-d experiment. Ingestion of alfalfa meal-contained diets significantly increased the ratio of body weight gain to feed consumption. Illumina MiS eq sequencing of the V3 region of the 16 S r RNA genes showed that alfalfa-containing diet significantly decreased the relative abundance of genera Turicibacter, Acidiphilium, Paracoccus, Propionibacterium,Corynebacterium, Pseudomonas, Acinetobacter, and Staphylococcus, and increased the relative abundance of genera Lachnospira, Marvinbryantia, and Desulfovibrio in the caecal digesta. Butyrate concentration was significantly increased in the hindgut by the supplementation of alfalfa meal in diets. The m RNA gene expressions of FFAR3, SMCT1, MCT1,PYY, and GCG were significantly increased in the caecal mucosa of pigs fed alfalfa meal.Conclusions: Our results suggested that alfalfa-containing diet has exerted significant impacts on caecal microbiota composition, butyrate concentration and significantly upregulated m RNA expression of host caecal mucosal genes involved in SCFA sensing and absorption as well as regulation of satiety. 关键词：ALFALFA meal Growing PIGS INSOLUBLE fiber MICROBIOTA SCFA

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Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs

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耦合文献(72)

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