| 1 | The Arabidopsis spliceosomal protein SmEb modulates ABA responses by maintaining proper alternative splicing of HAB1显示文摘Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal components in regulating ABA signaling is poorly understood.Here,we show that the spliceosome component Sm core protein SmEb plays an important role in ABA signaling.SmEb expression is up-regulated by ABA treatment,and analysis of Arabidopsis smeb mutant plants suggest that SmEb modulates the alternative splicing of the ABA signaling component HAB1 by enhancing the HAB1.1 splicing variant while repressing HAB1.2.Overexpression of HAB1.1 but not HAB1.2 rescues the ABA-hypersensitive phenotype of smeb mutants.Mutations in the transcription factor ABI3,4,or 5 also reduce the ABA hypersensitivity of smeb mutants during seed germination.Our results show that the spliceosomal component SmEb plays an important role in ABA regulation of seed germination and early seedling development. | Yechun Hong Juanjuan Yao Huazhong Shi Yunjuan Chen Jian-Kang Zhu Zhen Wang | 2021 | Stress Biology2021，1，1： | 1 |
| 2 | The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis显示文摘Salt stress adversely impacts crop production.Several spliceosome components have been implicated in regulating salt stress responses in plants,however,the underlying molecular basis is still unclear.Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis.Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress.Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants(RCD1.1/RCD1.2)important for salt stress response.In addition,RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates saltsensitivity by decreasing salt-induced cell death in smeb-1 mutant.Together,our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response. | Yechun Hong Yang Gao Jia Pang Huazhong Shi Tingting Li Huiying Meng Dali Kong Yunjuan Chen Jian-Kang Zhu Zhen Wang | 2023 | Journal of Integrative Plant Biology2023，65，6： | 0 |
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