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| 1 | Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis显示文摘Background: The poultry industry is in need of effective antibiotic alternatives to control outbreaks of necrotic enteritis(NE) due to Clostridium perfringens.Methods: This study was conducted to investigate the effects of feeding Bacil us coagulans on the growth performance and gut health of broiler chickens with C. perfringens-induced NE. Two hundred and forty 1-day-old broiler chicks were randomly assigned to a 2 × 2 factorial arrangement with two dietary B. coagulans levels(0 or 4 × 109 CFU/kg of diet) and two disease chal enge statuses(control or NE chal enged).Results: NE-induced reduction in body weight gain was relieved by the addition of B. coagulans into broiler diets compared with the NE-infected birds. NE infection damaged intestinal morphological structure, promoted intestinal C.perfringens growth and liver invasion, and enhanced anti-C. perfringens specific sI gA concentrations in the gut and specific IgG levels in serum compared with the uninfected birds. NE infection significantly(P < 0.05) decreased mucin-2(at 14 d post-infection(DPI), tol-like receptor 2(TLR2, at 7 and 14 DPI), TLR4(at 7 and 14 DPI), tumor necrosis factor super family15(TNFSF15, at 7 and 14 DPI), lysozyme(LYZ, at 14 DPI) and fowlicidin-2(at 7 and 14 DPI) mR NA levels, whereas it dramatical y(P = 0.001) increased IFN-γ mR NA levels at 7 DPI. However, chal enged birds fed diets supplemented with B.coagulans showed a significant(P < 0.01) decrease in gut lesion scores, decreased C. perfringens numbers in the cecum and liver, and an increase in fowlicidin-2 mR NA levels in compared with the uninfected birds. In addition, compared with the non-supplemented group, dietary inclusion of B. coagulans improved intestinal barrier structure, further increased specific sI gA levels and alkaline phosphatase(IAP) activity in the jejunum, enhanced the expression of jejunum lysozyme mR NA, and inhibited the growth, colonization, and invasion of C. perfringens; in contrast, it reduced serum-specific IgG concentrations and jejunum IFN-γ mR NA levels.Conclusion: These results indicated that dietary B. coagulans supplementation appeared to be effective in preventing the occurrence and reducing the severity of C. perfringens-induced NE in broiler chickens. | Yuanyuan Wu Yujing Shao Bochen Song Wenrui Zhen Zhong Wang Yuming Guo Muhammad Suhaib Shahid Wei Nie | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 31 |
| 2 | Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge显示文摘Three acid-producing strains,AFB-1,AFB-2and AFB-3,were isolated during this study,and their roles in anaerobic digestion of waste activated sludge (WAS)were evaluated.Data of 16S rRNA method showed that AFB-1 and AFB-2 were Bacillus coagulans,and AFB-3was Escherichia coil The removal in terms of volatile solids (VS)and total chemical oxygen demand (TCOD)was maximized at 42.7% and 44.7% by inoculating Bacillus coagulans AFB-1.Besides,the optimal inoculum concentration of Bacillus coagulans AFB-1was 30%(v/v).Solubilization degree expedments indicated that solubilization ratios (SR)of WAS reached 20.8%±2.2%,17.7%±1.48%,and 11.1%±1.53%.Volatile fatty acids (VFAs)concentrations and compositions were also explored with a gas chromatograph.The results showed that VFAs improved by 98.5%,53.0% and 11.6% than those of the eonlrol,respectively.Biochemical methane potential (BMP)experiments revealed that biogas production increased by 90.7%and 75.3%when inoculating with Bacillus coagulans AFB-1 and AFB-2.These results conftnned that the isolated acid-producing bacteria,especially Bacillus coagulans,was a good candidate for anaerobic digestion of WAS. | Sijia Ai Hongyu Liu Mengjie Wu Guangming Zeng Chunping Yang | 2018 | Frontiers of Environmental Science & Engineering2018，12，6： | 0 |
| 3 | 连续循环发酵生产L-乳酸新技术研究显示文摘通过对L-乳酸发酵动力学的研究,考察了发酵培养基(氮源、碳源),发酵工艺条件(温度、pH值、氧化还原电位ORP值),确定了乳酸发酵菌株JD.L-08的生长速率,建立了生长动力学方程μ=0.24s/(0.88+s),确定了菌株JD.L-08在连续培养中的稳定状态及发酵过程中的物质平衡,通过微滤装置的选择与操作条件优化(膜孔径、膜过滤压力、膜面积、纳虑膜的选择),在移出产物L-乳酸的同时实现了菌株的循环使用,确定了L-乳酸连续循环发酵生产的工艺条件。降低了发酵培养基的营养要求,提高了发酵的转化率及发酵罐的体积产率。 | 于培星 | 2010 | 中国食品添加剂2010，21，5： | 0 |
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