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| 1 | Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment显示文摘As an inhibitor of the Notch signaling pathway, N-[N-(3,5-difluorohenacetyl)-l-alanyl]-S-phenylglycine tert-butyl ester(DAPT) may protect brain tissue from serious ischemic injury. This study aimed to explore neuroprotection by DAPT after cerebral ischemia/reperfusion(I/R) injury. DAPT was intraperitoneally injected 3 hours before the establishment of a focal cerebral I/R model in the right middle cerebral artery of obstructed mice. Longa scores were used to assess neurological changes of mice. Nissl staining and TdT-mediated dUTP-biotin nick-end labeling staining were used to examine neuronal damage and cell apoptosis in the right prefrontal cortex, while immunofluorescence staining was used to detect glial fibrillary acidic protein-and Notch1-positive cells. Protein expression levels of Hes1 and Hes5 were detected by western blot assay in the right prefrontal cortex. Our results demonstrated that DAPT significantly improved neurobehavioral scores and relieved neuronal morphological damage. DAPT decreased the number of glial fibrillary acidic protein-and Notch1-positive cells in the right prefrontal cortex, while also reducing the number of apoptotic cells and decreasing interleukin-6 and tumor necrosis factor-α contents, and simultaneously downregulating Hes1 and Hes5 protein expression. These findings verify that DAPT alleviates pathological lesions and strengthens the anti-inflammatory response after cerebral I/R injury. Thus, DAPT might be developed as an effective drug for the prevention of cerebral I/R injury. | Jun-Jie Wang Jun-De Zhu Xian-Hu Zhang Ting-Ting Long Guo Ge Yan Yu | 2019 | Neural Regeneration Research2019，14，3： | 17 |
| 2 | Neural stem cell transplantation inhibits glial cell proliferation and P2X receptor-mediated neuropathic pain in spinal cord injury rats显示文摘P2X4 and P2X7 receptors play an important role in neuropathic pain after spinal cord injury. Regulation of P2X4 and P2X7 receptors can obviously reduce pain hypersensitivity after injury. To investigate the role of neural stem cell transplantation on P2X receptor-mediated neuropathic pain and explore related mechanisms, a rat model of spinal cord injury was prepared using the free-falling heavy body method with spinal cord segment 10 as the center. Neural stem cells were injected into the injured spinal cord segment using a micro-syringe. Expression levels of P2X4 and P2X7 receptors, neurofilament protein, and glial fibrillary acidic protein were determined by immunohistochemistry and western blot assay. In addition, sensory function was quantitatively assessed by current perception threshold. The Basso-Beattie-Bresnahan locomotor rating scale was used to assess neuropathological pain. The results showed that 4 weeks after neural stem cell transplantation, expression of neurofilament protein in the injured segment was markedly increased, while expression of glial fibrillary acidic protein and P2X4 and P2X7 receptors was decreased. At this time point, motor and sensory functions of rats were obviously improved, and neuropathic pain was alleviated. These findings demonstrated that neural stem cell transplantation reduced overexpression of P2X4 and P2X7 receptors, activated locomotor and sensory function reconstruction, and played an important role in neuropathic pain regulation after spinal cord injury. Therefore, neural stem cell transplantation is one potential option for relieving neuropathic pain mediated by P2X receptors. | Xiao-Jing Du Yue-Xia Chen Zun-Cheng Zheng Nan Wang Xiao-Yu Wang Fan-E Kong | 2019 | Neural Regeneration Research2019，14，5： | 6 |
| 3 | Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells显示文摘AIM To investigate the possible involvement of transient receptor potential vanilloid 1(TRPV1) in maturation of enteric glial cells(EGCs).METHODS Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus(MP) as well as cultured MP cells and EGCs using TRPV1 knockout(KO) mice.RESULTS We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type(WT) but not KO mice. Expression of glial fibrillary acidic protein(GFAP) immunoreactive signals was lower at postnatal day(PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100 B, another EGC/astrocyte-specific marker. CONCLUSION These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility. | Masahiro Yamamoto Mitsue Nishiyama Seiichi Iizuka Shigeaki Suzuki Norihiro Suzuki Sadakazu Aiso Jin Nakahara | 2016 | World Journal of Gastroenterology2016，22，44： | 0 |
| 4 | COMPARISON OF THE EFFECTS BETWEEN THE CONVENTIONAL AND ELECTRICAL ACUPUNCTURE ON SOME CYTOCHEMICAL COMPONENTS OF MEDIAN EMINENTIA AND SUPRAOPTIC NUCLEUS显示文摘Owing to the significant effectiveness onmany indications treated by puncturing,acupunc-ture has been widely adopted as a medical treat-ment in Europe,American and Japan etc.Electro-acupuncture(E.A.)was applied for su- | 葛子 王志英 贾林 | 1989 | Journal of Traditional Chinese Medicine1989，9，1： | 0 |
| 5 | Effects of electroacupuncture on the expressions of neuroal nitric oxide synthase and astrocyte in dentate gyrus of rats with Parkinson's disease显示文摘Objective To observe the changes in the expression of neuroal nitric oxide synthase (nNOS) and glial fibrillary acidic protein (GFAP) in dentate gyrus (DG) of rats with Parkinson’s disease (PD) and effects of electroacupuncture (EA) .Methods On the 7th day of | 丁艳霞 | 2013 | China Medical Abstracts(Internal Medicine)2013，30，3： | 0 |
| 6 | THE STUDIES ON MONOCLONAL ANTIBODY SZ-39 AGAINST HUMAN GLIOMA CELLS显示文摘A hybridoma cell line SZ-39 secreting monoclonal antibody against the human glioma cell has been established by a fusion between NS-1 myeloma cells and spleen cells from mice immunized with human glioma cell lines. Monoclonal antibody (McAb) SZ-39 was analyzed by ELISA, quantitative absorption, indirect immunofluorescence and ABC immunohistology. McAb SZ-39 strongly bound to 9/10 glioma cell lines, 17/20 glioma tissues, weakly bound to one liver cancer cell line and 1/2 lung cancer line, but they did not band with other tested human cancer linse. NcAb SZ-39 have no cross-reaction with lymphocyte, ABC red blood cells, white blood cells, blood platelet, normal bone marrow cells, fibroblast cells and 12 normal human tissues.The result indicated the antigen recognized by McAb SZ-39 may be a glioma-associated antigen <GAA). This GAA was analyzed by means of Western blotting. It was a MW 180 Kd glycopro-tein. The 131I-McAb SZ-39 specifically localized in human glioma xenografted in nude mice that indicate | 杨伟廉 杜子威 李佩霞 阮长耿 | 1989 | Chinese Journal of Cancer Research1989，1，3： | 0 |
| 7 | EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFT ER SPINAL INJURY IN ADULT RATS显示文摘Objective To study the ex pr ession of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were cr eated by artery clamp. Expression of Nestin and GFAP in different period (1,3,5d ays;1-8 weeks) and different area(injury locus and its surrounding tissue ) afte r spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5 00IW imaging processing system. Results There was expression of Nestin and GFAP in injured area 1 day after injury.The expression increased not only in in injured area but its sourrounding 3-7 days later and gradually got t o peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between e xpression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell may be invovled in the repair of central nervous system (CNS). | 屈建强 贺西京 杨平林 师蔚 李浩鹏 兰宾尚 袁普卫 王国毓 | 2004 | Journal of Pharmaceutical Analysis2004，16，2： | 0 |
| 8 | Effect of the control proliferation of astrocyte on the formation of glial scars by antisense GFAP retrovirus显示文摘Astrocytes play an important role in the formation of glial scars. in order to investigate the effect of inhibiting GFAP gene expression on normal, reactive astrocytes and on glial scar formation, the efficiency of the recombinant antisense GFAP retrovirus (PLBskG) on the growth, celi cycle, morphology and GFAP gene expression of astrocytes in vitro and on the formation of glial scars in vivo has been studied by celi growth curves, flow cytometry, immunocytochemistry, in situ hybridization, RT-PCR and Southern blot. The results confirm the recombinant retrovirus (PLBskG) produced growth suppression and G1 arrest of the normal and injured astrocytes. The infected cells become round or ellipoid. The celi processes become fine or retracted. The intensity of stain-ing of GFAP is reduced. Expression of GFAP mRNA is down regulated. However, in the control experiment, no obvious effects on the morphology or synthesis of GFAP on cultured normal and scratched astrocytes infected by primary retrovirus vector | HUANG Qilin CAI Wenqin ZHANG Kecheng | 2000 | Chinese Science Bulletin2000，45，1： | 0 |
| 9 | 胶质纤维酸性蛋白的免疫组化研究及其在肿瘤病理诊断中的应用(综述)显示文摘中间丝(Intermediate filaments,IFs)是细胞骨架的组成成份,是一组直径为7—11nm纤维状结构,主要包括五种类型:①角蛋白(keratin),存在于上皮细胞;②波形蛋白(Vimentin),存在于间叶起源的细胞及体外长期培养的细胞;③结蛋白(Desmin),存在于肌细胞;④神经微丝(Neurofilament),存在于神经细胞及其纤维;⑤胶质纤维酸性蛋白(Glial fibrillary acidic protein,GFAP),存在于神经胶质细胞[1,2,3]。 | 殷秀 范维珂 | 1990 | 川北医学院学报1990，5，3： | 0 |
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