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| 1 | Effects of Bacillus coagulans supplementation on the growth performance and gut health of broiler chickens with Clostridium perfringens-induced necrotic enteritis显示文摘Background: The poultry industry is in need of effective antibiotic alternatives to control outbreaks of necrotic enteritis(NE) due to Clostridium perfringens.Methods: This study was conducted to investigate the effects of feeding Bacil us coagulans on the growth performance and gut health of broiler chickens with C. perfringens-induced NE. Two hundred and forty 1-day-old broiler chicks were randomly assigned to a 2 × 2 factorial arrangement with two dietary B. coagulans levels(0 or 4 × 109 CFU/kg of diet) and two disease chal enge statuses(control or NE chal enged).Results: NE-induced reduction in body weight gain was relieved by the addition of B. coagulans into broiler diets compared with the NE-infected birds. NE infection damaged intestinal morphological structure, promoted intestinal C.perfringens growth and liver invasion, and enhanced anti-C. perfringens specific sI gA concentrations in the gut and specific IgG levels in serum compared with the uninfected birds. NE infection significantly(P < 0.05) decreased mucin-2(at 14 d post-infection(DPI), tol-like receptor 2(TLR2, at 7 and 14 DPI), TLR4(at 7 and 14 DPI), tumor necrosis factor super family15(TNFSF15, at 7 and 14 DPI), lysozyme(LYZ, at 14 DPI) and fowlicidin-2(at 7 and 14 DPI) mR NA levels, whereas it dramatical y(P = 0.001) increased IFN-γ mR NA levels at 7 DPI. However, chal enged birds fed diets supplemented with B.coagulans showed a significant(P < 0.01) decrease in gut lesion scores, decreased C. perfringens numbers in the cecum and liver, and an increase in fowlicidin-2 mR NA levels in compared with the uninfected birds. In addition, compared with the non-supplemented group, dietary inclusion of B. coagulans improved intestinal barrier structure, further increased specific sI gA levels and alkaline phosphatase(IAP) activity in the jejunum, enhanced the expression of jejunum lysozyme mR NA, and inhibited the growth, colonization, and invasion of C. perfringens; in contrast, it reduced serum-specific IgG concentrations and jejunum IFN-γ mR NA levels.Conclusion: These results indicated that dietary B. coagulans supplementation appeared to be effective in preventing the occurrence and reducing the severity of C. perfringens-induced NE in broiler chickens. | Yuanyuan Wu Yujing Shao Bochen Song Wenrui Zhen Zhong Wang Yuming Guo Muhammad Suhaib Shahid Wei Nie | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 31 |
| 2 | Effects of Lactobacillus acidophilus on the growth performance and intestinal health of broilers challenged with Clostridium perfringens显示文摘Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day trial.Results: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens challenge.Conclusion: Dietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis. | Zhui Li Weiwei Wang Dan Liu Yuming Guo | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 22 |
| 3 | A middle-aged lady with a pyogenic liver abscess caused by Clostridium perfringens显示文摘The pyogenic liver abscess caused by Clostridium perfringens (C. perfringens ) is a rare, but rapidly fatal infection. It is usually associated with malignancy and immunosuppression. We report the case of 50-year-old lady with the secondary liver metastases from rectal cancer presented with fever and epigastric pain. The identification of Grampositive bacilli septicaemia, the presence of gas-forming liver abscess and massive intravascular hemolysis should lead to the suspicion of C. perfringens infection. Here we review twenty cases published since 1990 and their clinical features are discussed. The importance of 'an aggressive treatment policy' with multidisciplinary team approach is emphasized. | Siu-Tong Law Ming Kai Lee | 2012 | World Journal of Hepatology2012，4，8： | 8 |
| 4 | Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide显示文摘Poultry production has undergone a substantial increase compared to the livestock industries since 1970.However, the industry worldwide is now facing challenges with the removal of in-feed antibiotics completely or gradually, as the once well-controlled poultry diseases have re-emerged to cause tremendous loss of production. Necrotic enteritis(NE) is one of the most important diseases which costs the industry over two billion dollars annually. In this paper, we review the progress on the etiology of NE and its control through dietary modifications, pre-and probiotics, short chain fatty acids, and vaccination. The other likely measures resulted in the most advances in the toxin characterization are also discussed. Vaccine strategies may have greater potential for the control of NE mainly due to clearer etiology of NE having been elucidated in recent years with the identification of necrotic enteritis toxin B-like(NetB) toxin. Therefore, the use of alternatives to in-feed antibiotics with a better understanding of the relationship between nutrition and NE, and limiting exposure to infectious agents through biosecurity and vaccination, might be a tool to reduce the incidence of NE and to improve gut health in the absence of in-feed antibiotics. More importantly, the combinations of different measures may achieve greater protection of birds against the disease. Among all the alternatives investigated, prebiotics, organic acids and vaccination have shown improved gastrointestinal health and thus, have potential for the control of NE. | Shawkat A.M'Sadeq Shubiao Wu Robert A.Swick Mingan Choct | 2015 | Animal Nutrition2015，，1： | 6 |
| 5 | Gas-forming liver abscess associated with rapid hemolysis in a diabetic patient显示文摘We experienced a case of liver abscess due to Clostridium perfringens(CP) complicated with massive hemolysis and rapid death in an adequately controlled type2 diabetic patient. The patient died 6 h after his first visit to the hospital. CP was later detected in a blood culture. We searched for case reports of CP septicemia and found 124 cases. Fifty patients survived, and 74died. Of the 30 patients with liver abscess, only 3 cases survived following treatment with emergency surgica drainage. For the early detection of CP infection, detection of Gram-positive rods in the blood or drainage fluid is important. Spherocytes and ghost cells indicate intravascular hemolysis. The prognosis is very poor once massive hemolysis occurs. The major causative organisms of gas-forming liver abscess in diabetic patients are Klebsiella pneumoniae(K. pneumoniae) and Escherichia coli(E. coli). Although CP is relatively rare,the survival rate is very poor compared with those of K. pneumoniae and E. coli. Therefore, for every case that presents with a gas-forming liver abscess, the possibility of CP should be considered, and immediate aspiration of the abscess and Gram staining are important. | Miwa Kurasawa Takashi Nishikido Junko Koike Shin-ichi Tominaga Hiroyuki Tamemoto | 2014 | World Journal of Diabetes2014，5，2： | 4 |
| 6 | A secondary bile acid from microbiota metabolism attenuates ileitis and bile acid reduction in subclinical necrotic enteritis in chickens显示文摘Background:Clostridium perfringens-induced chicken necrotic enteritis(NE)is responsible for substantial economic losses worldwide annually.Recently,as a result of antibiotic growth promoter prohibition,the prevalence of NE in chickens has reemerged.This study was aimed to reduce NE through titrating dietary deoxycholic acid(DCA)as an effective antimicrobial alternative.Materials and methods:Day-old broiler chicks were assigned to six groups and fed diets supplemented with 0(basal diet),0.8,1.0 and 1.5 g/kg(on top of basal diet)DCA.The birds were challenged with Eimeria maxima(20,000 oocysts/bird)at d 18 and C.perfringens(109 CFU/bird per day)at d 23,24,and 25 to induce NE.The birds were sacrificed at d 26 when ileal tissue and digesta were collected for analyzing histopathology,mRNA accumulation and C.perfringens colonization by real-time PCR,targeted metabolomics of bile acids,fluorescence in situ hybridization(FISH),or terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay.Results:At the cellular level,birds infected with E.maxima and C.perfringens developed subclinical NE and showed shortening villi,crypt hyperplasia and immune cell infiltration in ileum.Dietary DCA alleviated the NE-induced ileal inflammation in a dose-dependent manner compared to NE control birds.Consistent with the increased histopathological scores,subclinical NE birds suffered body weight gain reduction compared to the uninfected birds,an effect attenuated with increased doses of dietary DCA.At the molecular level,the highest dose of DCA at 1.5 g/kg reduced C.perfringens luminal colonization compared to NE birds using PCR and FISH.Furthermore,the dietary DCA reduced subclinical NE-induced intestinal inflammatory gene expression and cell apoptosis using PCR and TUNEL assays.Upon further examining ileal bile acid pool through targeted metabolomics,subclinical NE reduced the total bile acid level in ileal digesta compared to uninfected birds.Notably,dietary DCA increased total bile acid and DCA levels in a dose-dependent manner compared to NE birds.Conclusion:These results indicate that DCA attenuates NE-induced intestinal inflammation and bile acid reduction and could be an effective antimicrobial alternative against the intestinal disease. | Mohit Bansal Ying Fu Bilal Alrubaye Mussie Abraha Ayidh Almansour Anamika Gupta Rohana Liyanage Hong Wang Billy Hargis Xiaolun Sun | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 3 |
| 7 | 应用梭状芽孢杆菌口服疫苗载体表达HIV p24蛋白的研究显示文摘应用一种不产毒的梭状芽孢杆菌(Clostridium perfringens,C.P.)作为口服疫苗载体表达HIVp24蛋白。用PCR扩增HIVp24基因,酶切后插入到含有C.P.序列的pJRC200质粒的CPEORF中,将重组的cp24质粒转化无致病作用的ATCC3624菌株。使HIVp24基因在C.P.形成芽孢时大量表达。结果应用SDS_PAGE和West Blot分析HIVp24在重组C.P.繁殖体和芽孢中的表达情况,发现HIVp24蛋白在C.P.芽孢体中大量表达,而在C.P.繁殖体中却没有表达。证明在体外构建了含有HIVp24基因的重组pJRC200质粒,成功地表达了HIVp24蛋白并得到了HIVp24的高表达ATCC3624菌株。为成功研究C.P.作为一种口服HIV疫苗载体奠定了基础。 | 石英 周育森 郭彦 肖文君 于虹 吴昊 陈月 PhalguniGupta | 2007 | 科学技术与工程2007，7，17： | 3 |
| 8 | Emphysematous cholecystitis with massive gas in the abdominal cavity显示文摘Emphysematous cholecystitis is a rare variant of acute cholecystitis with a high mortality rate.The combination of emphysematous cholecystitis and pneumoperitoneum is also rare.We herein describe a case of emphysematous cholecystitis with massive gas in the abdominal cavity.A 77-year-old male presented with epigastric pain and lassitude lasting for one week.A computed tomography scan demonstrated massive gas in the abdominal cavity.Gas was also detectable inside the gallbladder.Massive ascites as well as a pleural effusion were also detected.Under the diagnosis of perforation of the digestive tract,we performed emergency surgery.Beyond our expectations,the perforation site was not in the alimentary tract,but rather in the gallbladder.We then diagnosed the patient with emphysematous cholecystitis with perforation,and performed cholecystectomy.A pathological examination of the resected gallbladder revealed necrosis in the mucosa and thinning of the wall.Cultures of the ascites detected Clostridium perfringens,a gas-producing microorganism. | Hiroyuki Miyahara Dai Shida Hiroki Matsunaga Yukiko Takahama Sachio Miyamoto | 2013 | World Journal of Gastroenterology2013，19，4： | 2 |
| 9 | B型产气荚膜梭菌三种主要毒素基因的PCR检测显示文摘为了对B型产气荚膜梭菌(Clostridium perfringens type B)进行快速检测,依据NCBI登录的产气荚膜梭菌α、β及ε毒素基因序列设计引物,并对其进行PCR扩增。琼脂糖凝胶电泳结果显示3个毒素基因均被成功扩增,目标片段条带清晰,长度与预期相符。 | 冶贵生 | 2012 | 湖北农业科学2012，51，13： | 1 |
| 10 | Efficient and Soluble Expression of α Protein of Clostridium perfringens and Preparation of Genetic Engineering Subunit Vaccine显示文摘[Objective] The paper was to develop genetic engineering vaccine that can express α exotoxin antigen protein efficiently without destroying its immunogenicity for preventing and controlling the diseases caused by Clostridium perfringens. [Method] Efficiently expressed soluble recombinant α protein was obtained from Escherichia coli expression system by optimizing codon,removing signal peptide,selecting sequences with better hydrophilicity and antigenicity,and optimizing expression conditions. [Result] Mice obtained higher serum antibody level when immunized by α protein,and the immune protection rates against type A,type B,type C and type D C. perfringens were 100%,90%,85% and 90%,respectively. The antibody titer of mice within 7-14 d after the third immunization reached the peak. [Conclusion]The α protein has good immunogenicity,and can be further used to develop genetic engineering subunit vaccines for preventing C. perfringens. | Sun Yu Yang Lin Wang Chuanbin Dong Hao Qu Ping Zhao Bolin Hu Dongmei Yang Tianyi Song Xiaohui | 2017 | Animal Husbandry and Feed Science2017，9，5： | 0 |
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