|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cystine transporter SLC7A11/xCT in cancer:ferroptosis,nutrient dependency,and cancer therapy显示文摘The cystine/glutamate antiporter SLC7A11(also commonly known as xCT)functions to import cystine for glutathione biosynthesis and antioxidant defense and is overexpressed in multiple human cancers.Recent studies revealed that SLC7A11 overexpression promotes tumor growth partly through suppressing ferroptosis,a form of regulated cell death induced by excessive lipid peroxidation.However,cancer cells with high expression of SLC7A11(SLC7A11^(high))also have to endure the significant cost associated with SLC7A11-mediated metabolic reprogramming,leading to glucose-and glutamine-dependency in SLC7A11^(high) cancer cells,which presents potential metabolic vulnerabilities for therapeutic targeting in SLC7A11^(high) cancer.In this review,we summarize diverse regulatory mechanisms of SLC7A11 in cancer,discuss ferroptosis-dependent and-independent functions of SLC7A11 in promoting tumor development,explore the mechanistic basis of SLC7A11-induced nutrient dependency in cancer cells,and conceptualize therapeutic strategies to target SLC7A11 in cancer treatment.This review will provide the foundation for further understanding SLC7A11 in ferroptosis,nutrient dependency,and tumor biology and for developing novel effective cancer therapies. | Pranavi Koppula Li Zhuang Boyi Gan | 2021 | Protein & Cell2021，12，8： | 97 |
| 2 | Amino acid transporter SLC7A11/ xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer显示文摘Cancer cells often upregulate nutrient transporters to fulfill their increased biosynthetic and bioenergetic needs,and to maintain redox homeostasis.One nutrient transporter frequently overexpressed in human cancers is the cystine/glutamate antiporter solute carrier family 7 member 11(SLC7A11;also known as xCT).SLC7A11 promotes cystine uptake and glutathione biosynthesis,resulting in protection from oxidative stress and ferroptotic cell death.Recent studies have unexpectedly revealed that SLC7A11 also plays critical roles in glutamine metabolism and regulates the glucose and glutamine dependency of cancer cells.This review discusses the roles of SLC7A11 in regulating the anti-oxidant response and nutrient dependency of cancer cells,explores our current understanding of SLC7A11 regulation in cancer metabolism,and highlights key open questions for future studies in this emerging research area.A deeper understanding of SLC7A11 in cancer metabolism may identify new therapeutic opportunities to target this important amino acid transporter for cancer treatment. | Pranavi Koppula Yilei Zhang Li Zhuang Boyi Gan | 2018 | Cancer Communications2018，38，1： | 77 |
| 3 | Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis显示文摘Ferroptosis is an iron-dependent novel cell death pathway. Deferoxamine, a ferroptosis inhibitor, has been reported to promote spinal cord injury repair. It has yet to be clarified whether ferroptosis inhibition represents the mechanism of action of Deferoxamine on spinal cord injury recovery. A rat model of Deferoxamine at thoracic 10 segment was established using a modified Allen's method. Ninety 8-week-old female Wistar rats were used. Rats in the Deferoxamine group were intraperitoneally injected with 100 mg/kg Deferoxamine 30 minutes before injury. Simultaneously, the Sham and Deferoxamine groups served as controls. Drug administration was conducted for 7 consecutive days. The results were as follows:(1) Electron microscopy revealed shrunken mitochondria in the spinal cord injury group.(2) The Basso, Beattie and Bresnahan locomotor rating score showed that recovery of the hindlimb was remarkably better in the Deferoxamine group than in the spinal cord injury group.(3) The iron concentration was lower in the Deferoxamine group than in the spinal cord injury group after injury.(4) Western blot assay revealed that, compared with the spinal cord injury group, GPX4, xCT, and glutathione expression was markedly increased in the Deferoxamine group.(5) Real-time polymerase chain reaction revealed that, compared with the Deferoxamine group, mRNA levels of ferroptosis-related genes Acyl-CoA synthetase family member 2(ACSF2) and iron-responsive element-binding protein 2(IREB2) were up-regulated in the Deferoxamine group.(6) Deferoxamine increased survival of neurons and inhibited gliosis. These findings confirm that Deferoxamine can repair spinal cord injury by inhibiting ferroptosis. Targeting ferroptosis is therefore a promising therapeutic approach for spinal cord injury. | Xue Yao Yan Zhang Jian Hao Hui-Quan Duan Chen-Xi Zhao Chao Sun Bo Li Bao-You Fan Xu Wang Wen-Xiang Li Xuan-Hao Fu Yong Hu Chang Liu Xiao-Hong Kong Shi-Qing Feng | 2019 | Neural Regeneration Research2019，14，3： | 35 |
| 4 | 基于三维XCT对碳化引起水泥砂浆内部缺陷改变的测试和分析(英文)显示文摘为了揭示碳化反应对水泥砂浆内部缺陷分布的影响规律,采用三维XCT(X-ray computed tomography)对碳化前后的水泥砂浆的三维内部缺陷体积分数和缺陷尺度分布进行了定量分析。通过XCT的配套软件VG Studio MAX 2.0对水泥砂浆内部缺陷的投影进行三维重构,并通过配套的三维缺陷分析模块软件从三维图像中提取出水泥砂浆内部缺陷的体积分数和尺度分布。结果表明:三维缺陷尺寸范围为0.03~5.5 mm3,碳化前后的水泥砂浆内部缺陷体积分数分别为2.79%和1.86%。体积小的缺陷减小的比例更大,特别在0.03~0.1 mm3的缺陷体积减小的比例最大。主要原因是水泥砂浆中的氢氧化钙和CSH凝胶碳化反应后生成碳酸钙和水,相同摩尔质量的碳酸钙的体积比氢氧化钙和CSH凝胶都要大,碳酸钙和水填堵并细化了原来的缺陷,导致碳化后水泥砂浆内部缺陷体积分数减小。 | 韩建德 潘钢华 孙伟 | 2011 | 硅酸盐学报2011，39，1： | 17 |
| 5 | SLC7A11基因在恶性肿瘤中的研究进展显示文摘SLC7A11为溶质载体家族成员之一,该基因编码胱氨酸/谷氨酸反转运体xc-系统的轻链亚基SLC7A11(又称xCT)。 SLC7A11通过介导胱氨酸摄取和谷氨酸释放促进谷胱甘肽(glutathione,r-glutamyl cysteingl+glycine,GSH)的 合成,保护细胞免受 氧化应激,维持细胞的氧化还原平衡,阻止脂质过氧化诱导的细胞死亡。SLC7A11在多种恶性肿瘤中过表达,与胶质瘤、乳腺癌、 卵巢癌、肝癌和肺癌等恶性肿瘤的生长、预后、转移和治疗密切相关,为恶性肿瘤新的潜在治疗靶点之一。本文就SLC7A11基因 在恶性肿瘤中的研究进展进行综述。 | 赵雨霏 陶圆 颜晓菁 | 2019 | 中国肿瘤临床2019，46，15： | 12 |
| 6 | A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells显示文摘Breast cancer is one of the most malignant tumors and is associated with high mortality rates among women.Lycium barbarum polysaccharide(LBP)is an extract from the fruits of the traditional Chinese herb,L.barbarum.LBP is a promising anticancer drug,due to its high activity and low toxicity.Although it has anticancer properties,its mechanisms of action have not been fully established.Ferroptosis,which is a novel anticancer strategy,is a cell death mechanism that relies on iron-dependent lipid reactive oxygen species(ROS)accumulation.In this study,human breast cancer cells(Michigan Cancer Foundation-7(MCF-7)and MD Anderson-Metastatic Breast-231(MDA-MB-231))were treated with LBP.LBP inhibited their viability and proliferation in association with high levels of ferroptosis.Therefore,we aimed to ascertain whether LBP reduced cell viability through ferroptosis.We found that the structure and function of mitochondria,lipid peroxidation,and expression of solute carrier family 7 member 11(SLC7 A11,also known as x CT,the light-chain subunit of cystine/glutamate antiporter system X_(c)^(-))and glutathione peroxidase 4(GPX4)were altered by LBP.Moreover,the ferroptosis inhibitor,Ferrostatin-1(Fer-1),rescued LBP-induced ferroptosis-associated events including reduced cell viability and glutathione(GSH)production,accumulation of intracellular free divalent iron ions and malondialdehyde(MDA),and down-regulation of the expression of x CT and GPX4.Erastin(x CT inhibitor)and RSL3(GPX4 inhibitor)inhibited the expression of x CT and GPX4,respectively,which was lower after the co-treatment of LBP with Erastin and RSL3.These results suggest that LBP effectively prevents breast cancer cell proliferation and promotes ferroptosis via the x CT/GPX4 pathway.Therefore,LBP exhibits novel anticancer properties by triggering ferroptosis,and may be a potential therapeutic option for breast cancer. | Xing DU Jingjing ZHANG Ling LIU Bo XU Hang HAN Wenjie DAI Xiuying PEI Xufeng FU Shaozhang HOU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，4： | 10 |
| 7 | NADPH debt drives redox bankruptcy:SLC7A11/xCT-mediated cystine uptake as a double-edged sword in cellular redox regulation显示文摘Cystine/glutamate antiporter solute carrier family 7 member 11(SLC7A11;also known as xCT)plays a key role in antioxidant defense by mediating cystine uptake,promoting glutathione synthesis,and maintaining cell survival under oxidative stress conditions.Recent studies showed that,to prevent toxic buildup of highly insoluble cystine inside cells,cancer cells with high expression of SLC7A11(SLC7A11high)are forced to quickly reduce cystine to more soluble cysteine,which requires substantial NADPH supply from the glucose-pentose phosphate pathway(PPP)route,thereby inducing glucose-and PPP-dependency in SLC7A11high cancer cells.Limiting glucose supply to SLC7A11high cancer cells results in significant NADPH“debt”,redox“bankruptcy”,and subsequent cell death.This review summarizes our current understanding of NADPH-generating and-consuming pathways,discusses the opposing role of SLC7A11 in protecting cells from oxidative stresseinduced cell death such as ferroptosis but promoting glucose starvationeinduced cell death,and proposes the concept that SLC7A11-mediated cystine uptake acts as a double-edged sword in cellular redox regulation.A detailed understanding of SLC7A11 in redox biology may identify metabolic vulnerabilities in SLC7A11high cancer for therapeutic targeting. | Xiaoguang Liu Yilei Zhang Li Zhuang Kellen Olszewski Boyi Gan | 2021 | Genes & Diseases2021，8，6： | 7 |
| 8 | 含能材料密度的XCT自参照测试显示文摘运用同步测试技术和体积CT灰度值计算方法,研究含能材料的体密度与体积CT灰度值的线性关系。以密度相近的含能材料试验件组成的体系密度作为参照,利用各试验件的VCT灰度值计算CT密度值。试验结果表明,采用自参照密度测试方法可以简化含能材料局部密度均匀性的测试,CT法计算获得的试验件密度值与排水法测得密度值的相对误差<0.1%。 | 杨雪海 张伟斌 戴斌 田勇 | 2010 | 无损检测2010，32，6： | 6 |
| 9 | 基于XCT技术的原位根系三维可视化研究显示文摘为解决植物根系原位三维观测的技术难题,采用XCT层析成像技术获取原位根系的断层序列图像,然后利用计算机图像图形处理技术实现对植物根系的原位三维可视化。为了提高图像分割的精确性,提出一种利用根系几何形态特征的综合分割方法,有效清除了与根系密度极其近似的杂质体素,完成了对序列图像的三维分割。并利用VTK工具箱采用移动立方体算法(MC)实现了对分割后序列图像的三维重建。编程实验证实,本文提出的技术路线和方法能够有效地实现对生长在介质环境中原位根系的三维可视化观测。 | 周学成 罗锡文 | 2009 | 农业机械学报2009，40，S1： | 5 |
| 10 | xCT和促血管生成因子在胶质瘤组织中肿瘤相关巨噬细胞的表达及意义显示文摘目的:探讨xCT和促血管生成因子在胶质瘤组织中肿瘤相关巨噬细胞(TAMs)中的表达,以及TAMs对胶质瘤进展的影响。方法:分离并培养人新鲜胶质瘤和正常脑组织中的TAMs。采用荧光实时定量(RT-PCR)技术检测M2表型标记物CD14和CD86,促血管生成因子Arg-1和CD209,以及xCT mRNA表达水平。组织冰冻切片免疫荧光染色检测CD68和xCT在胶质瘤中的表达。结果:xCT和CD68在胶质母细胞瘤(WHO°Ⅳ)中的表达明显高于WHO°Ⅱ胶质瘤和正常脑组织,且xCT和CD68共同表达于小胶质细胞中。xCT mRNA在分化程度差的胶质瘤中表达明显增高。此外,小胶质细胞M2表型标记物表达水平(CD14和CD86)、活化状态的TAMs数目和促血管生成因子(Arg-1和CD209)与肿瘤分级相关。结论:TAMs能够通过xCT过表达,促炎症因子和促血管生成因子促进胶质瘤的发展。肿瘤中M2表型的TAMs募集与脑胶质瘤的不良预后相关。 | 陈黛诗 郑朝攀 杜甲珺 胡继良 | 2018 | 中国免疫学杂志2018，34，9： | 4 |
| 11 | 基于XCT序列图像的植物根系三维矢量模型构建方法显示文摘为实现植物根系三维构型参数的原位、无损、准确、快速和自动化测量,提出一种基于X射线计算机断层摄影术(X-ray computed tomography,简称XCT)序列图像的植物根系三维矢量模型构建方法。首先,根据根系的形态特征设计了根结点、根分枝、根系统3个基本结构;其次,对植物根系三维体数据(XCT序列图像)重切以提取根截面,并根据根截面质心和面积构建初始根结点,继而对根结点分组以构建初始根分枝,再通过重构根结点、重获根结点面积和根分枝关系判定构建完整的根系统(矢量模型);再次,基于矢量模型设计根数、根长度、根体积、根表面积以及根夹角的计算方法;最后,在Windows平台上利用图形用户界面开发工具Qt、可视化工具包VTK和医学图像分割与配准工具包ITK实现上述结构和算法,并将上述参数的计算结果与手工测量值进行对比,验证所提出方法的可行性与准确性。 | 李一海 周学成 陈富强 | 2017 | 江苏农业科学2017，45，14： | 3 |
| 12 | 断层扫描成像新技术——光学相干计算机断层摄影术显示文摘断层扫描成像技术是人类疾病诊断学中的重要工具。人们所熟知的X线计算机断层摄影(XCT)和磁共振成像(MRI)等技术,已在生物医学领域中得到了广泛应用,在医学诊断中,尤显重要。但是由于技术能力所限,XCT和MRI无法使人们观察到密集体腔内的组织变异,空... | 薛平 钱俊雯 刘志荣 陈瓞延 | 1999 | 中国激光医学杂志1999，8，2： | 3 |
| 13 | 安捷伦液相/离子阱(XCT)质谱检测虾仁及家禽中的硝基呋喃代谢物显示文摘在液相/离子阱质谱上,利用液相/质谱/质谱方法对鸡肉及虾仁中硝基呋喃代谢物残留进行了定性定量分析.对于四种硝基呋喃代谢物,其定量限在0125到05μg·kg-1的范围内,分析结果完全满足欧盟的1μg·kg-1的最低检测限量的要求. | Bernhard wüst Christian Sauber Hans (J) A van Rhijn | 2004 | 环境化学2004，23，6： | 2 |
| 14 | xCT与肿瘤和肿瘤干细胞关系的研究进展显示文摘胱氨酸/谷氨酸逆向转运体(system xc-)是细胞膜上的逆向转运氨基酸的异源二聚体,参与氧化还原和氨基酸转运的调控。胱氨酸/谷氨酸反向运输溶质载体家族7成员11(SLC7A11)也称为xCT,为其主要功能性亚基。由于肿瘤组织中氧化应激的增强及营养代谢需求的增加,xCT在多种肿瘤细胞中呈异常表达。在肿瘤细胞中,xCT通过摄取胱氨酸还原为半胱氨酸用于谷胱甘肽的合成,参与肿瘤耐药;同时胞浆内多余的半胱氨酸分泌到细胞外,提供细胞信号传导和细胞间通讯所需的还原微环境,有利于肿瘤细胞的增殖、侵袭和转移。在肿瘤干细胞(CSC)中,xCT促进CSC表面标记蛋白的表达,与维持CSC的干性特征密切相关,是肿瘤治疗的潜在靶点之一,并且有望应用于肿瘤的临床筛查。现通过回顾近年来xCT的相关研究,就xCT在肿瘤和CSC中的生物学作用及其作用机制进行综述。 | 熊芳 龙梅芳 叶小群 | 2019 | 吉林大学学报(医学版)2019，45，6： | 2 |
| 15 | 蓝光诱导视网膜色素上皮细胞铁死亡的机制研究显示文摘目的:探讨蓝光诱导人视网膜色素上皮(ARPE)细胞铁死亡的发生及可能机制。方法:体外培养的ARPE-19细胞接受405nm蓝光50mW/cm^(2)辐照度照射不同时间,分为对照组、16.3J/cm^(2)组、32.6J/cm^(2)组和65.2J/cm^(2)组;将65.2J/cm^(2)组定为高能量蓝光照射组,进一步分为对照组、高能量蓝光照射组和高能量蓝光照射+铁死亡抑制剂组,CCK-8检测细胞活力,试剂盒检测细胞内谷胱甘肽(GSH)含量、二价铁离子浓度及丙二醛(MDA)含量,Western blot法检测细胞内GPX4和xCT蛋白相对表达量。结果:蓝光照射导致ARPE-19细胞活力下降呈剂量依赖性,高能量蓝光照射导致细胞内GSH含量下降,二价铁离子浓度和MDA含量上升(均P<0.05);加入铁死亡抑制剂可部分恢复蓝光照射组细胞活力和GSH含量,减少MDA含量,降低二价铁离子浓度(均P<0.05);蓝光照射组GPX4和xCT蛋白相对表达量显著下降,加入铁死亡抑制剂后蛋白表达量不同程度恢复(P<0.05)。结论:蓝光照射可能通过影响xCT和GPX4相关抗氧化途径诱导RPE细胞铁死亡发生。 | 徐翌华 徐丽 蒋晶晶 朱鸿 | 2023 | 国际眼科杂志2023，23，4： | 2 |
| 16 | 工业CT坐标精密测量技术显示文摘介绍了基于X射线工业CT的坐标精密测量技术,讨论了该技术相对于传统三坐标测量技术的优势和不足及国内外的发展现状,对主要研究方向进行了探讨和研究,指出目前CT坐标测量中存在的问题,为评价测量不确定度提出了系统的解决思路。工业CT坐标精密测量测量技术在国内尚处于探索阶段,具有较大的发展空间和应用前景。 | 苏宇航 王倩妮 何方成 | 2015 | 计量学报2015，36，4： | 2 |
| 17 | 牙龈卟啉单胞菌通过xCT受体调控食管癌细胞谷氨酰胺代谢显示文摘目的探讨牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)通过膜受体分子-溶质运载蛋白7家族成员11(solute cartier family 7 member 11,SLC7A11,又称xCT)调节食管鳞癌细胞谷氨酰胺代谢的作用及机制。方法基于人类全基因组库筛选Pg感染食管鳞癌细胞所需的宿主因子;运用免疫共沉淀、NanoBiT活细胞实时蛋白相互作用和免疫荧光实验探索Pg的主要毒力因子鞭毛蛋白(fimbriae,FimA)和宿主细胞受体分子xCT之间的相互作用关系;构建xCT基因敲除小鼠模型,Pg口腔灌胃4周后剥离小鼠食管组织,利用RNAscope原位杂交检测小鼠食管黏膜Pg的载菌量;采用qRT-PCR、Western blot和还原型/氧化型谷胱甘肽试剂盒测定Pg感染对食管癌细胞内谷氨酰胺代谢及其合成酶表达的影响。结果全基因组筛选测序分析及细胞活力测定结果表明,xCT能够增强Pg在胞内的定殖从而促进食管癌细胞增殖;免疫共沉淀、NanoBiT实验和免疫细胞荧光实验结果表明,xCT与Pg鞭毛蛋白FimA可直接结合并相互作用;体内实验结果表明,xCT基因敲除小鼠食管上皮中Pg载菌量显著低于野生型小鼠(P<0.01);qRT-PCR、Western blot和还原型/氧化型谷胱甘肽测定发现,感染Pg后食管癌细胞的谷氨酰胺合成酶(glutaminase-1,GLS1)以及还原型/氧化型谷胱甘肽比率显著高于未感染组,差异具有统计学意义(均P<0.01)。结论xCT是Pg入侵食管癌细胞的重要受体分子之一;Pg入胞后能显著增强肿瘤细胞谷氨酰胺代谢。上述研究为食管癌的病因学和防治策略提供了理论依据。 | 张秀森 刘书培 王海瑞 原翔 | 2022 | 华中科技大学学报(医学版)2022，51，4： | 2 |
| 18 | 在CT扫描中X射线辐射场均匀性的理论计算显示文摘由于具有无痛、高效等优势,XCT被广泛应用于医疗方面.X射线辐射场的均匀性对图像的质量产生重要影响.利用C语言编制计算机程序,其中的参数取自医用XCT的技术参数,以探讨靶角、管电压、距离和过滤材料如何影响反射式X射线管辐射场的均匀性. | 李志 | 2005 | 杭州师范学院学报(自然科学版)2005，4，5： | 1 |
| 19 | GE lightspeed 5.X CT OC部分故障分析与维修显示文摘GE lightspeed5.XCT在正常情况下开机会启动Linux后自动进入应用程序,对各部件自检复位后进入扫描界面。但OC如有部件出现故障时机器将出现报警提示而中断应用程序的运行。下面是两例因DARC原因引起不能正常开机的故障。 | 罗焕江 | 2009 | 北京生物医学工程2009，28，4： | 1 |
| 20 | Effects of cooling rates on microporosity in DC casting Al-Li alloy显示文摘During the direct chill(DC)casting process,primary cooling from the mold and bottom block,and secondary cooling from the waterjets produce a concave solid shell.The depth of this liquid pocket and mushy zone not only depends on the solidification range of the alloy but also the boundary conditions such as cooling rates.Al-Li alloys solidify in a long solidification range increasing the susceptibility of porosity nucleation in the semi-solid region.In this study,the effects of cooling rate on the porosity formation were quantified for the large ingot casting using X-ray computed tomography(XCT).By characterizing pore size distributions at four different cooling conditions,the correlation between the mechanical properties at both room and high temperatures and the microstructure features was identified.The constitutive equations were constructed.It is found that increasing the cooling rate reduces the grain size,increases the number density of micropores,and minimizes the number of large pores,thereby improving the mechanical performance.Therefore,long mushy zones and deep liquid pockets in Al-Li alloys can be effectively controlled by controlling the boundary conditions of the DC casting solidification process,thereby obtaining castings with excellent mechanical properties. | Yu-xuan Zhang Jun-sheng Wang Dong-xu Chen Bing Wang Chi Zhang Zheng-an Wang | 2022 | China Foundry2022，19，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员