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Experimental study on filtering,transporting, concentrating and focusing of microparticles based on optically induced dielectrophoresis

查看全文 作  者:ZHU XiaoLu,YIN ZhiFeng,GAO ZhiQiang & NI ZhongHua Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments,Southeast University,Nanjing 211189,China 高影响力作者 出  处:《Science China(Technological Sciences)》索引2010年第53卷第9期,共9页高影响力期刊 基  金:supported by the National High Technology Research and Development Program of China ('863' Program) (Grant No. 2009AA04Z310);the National Natural Science Foundation of China (Grant Nos. 30770553, 50805022) 摘  要:The key problem to be solved for the dielectrophoresis (DEP) application is to provide dynamically reconfigurable microelectrodes and low-cost methodology for bioparticle manipulation.The emergence of optically induced DEP (ODEP) based on photoconductive effect provides a potential solution for the above problem.In this paper,an ODEP chip is designed and fabricated,and the corresponding experimental platform was established,whereupon four types of particle manipulation regimes–filtering,transporting,concentrating and focusing based on ODEP are experimentally demonstrated and the operating performances are quantitatively analyzed.The experiment results show that the functions and performances of ODEP manipulation are heavily dependent on the geometrical shape,scales and speed of optical patterns,actuating signal frequency and the electric conductivity of the solution.The manipulation efficiency can increase by more than 50% via increasing the optical line width.Moreover,the efficiency is obviously affected by the inclination angle of the optical oblique lines in the manipulation of particle focusing.Additionally,the maximum velocity of particles increases with the increment of the inside radius and the thickness of the optical trapping ring.Particle manipulation efficiency is always related to signal frequency and solution conductivity,and empirically,satisfactory performance and high efficiency are obtained when the solution electric conductivity ranges from 5×10-4 S/m to 5×10-3 S/m. 关 键 词:optically-induced dielectrophoresis particle MANIPULATION FILTERING transporting CONCENTRATING FOCUSING
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