Geographical distribution of GmTfl1 alleles in Chinese soybean varieties

查看全文 作　　者：Guifeng [1]Liu;Lin [1]Zhao;Benjamin [2]J.Averitt;Ying [3]Liu;Bo [2]Zhang;Ruzhen [3]Chang;Yansong [4]Ma;Xiaoyan [4]Luan;Rongxia [3]Guan;Lijuan [1,3]Qiu 机构地区：[1]Soybean Research Institute, Northeast Agricultural University;[2]Department of Crop and Soil Environmental Sciences, VA Polytechnic Institute and State University;[3]The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science,Chinese Academy of Agricultural Sciences;[4]Heilongjiang Academy of Agricultural Sciences 出　　处：《The Crop Journal》索引2015年第3卷第5期,共8页 基　　金：supported by the National Natural Science Foundation of China(31271753);the National Key Technology R&D Program of China(2012AA101106);the Agricultural Science and Technology Innovation Program(ASTIP);Development and Application of Molecular Markers in Crops;the Agricultural Science and Technology Innovation Program;the National Key Basic Research Program of China(2009CB118400);the Crop Germplasm Resources Protection(2014NWB030,2015NWB030-05);the Platform of National Crop Germplasm Resources of China(2014-004,2015-004) 摘　　要：Stem growth habit is an important agronomic trait in soybean and is subject to artificial selection. This study aimed to provide a theory for genotypic selection of stem growth habit for breeding purposes by analyzing the alleles of Gm Tfl1 gene in Chinese soybean varieties and establishing a database of Gm Tfl1 variation. Using knowledge of insertion and deletion(Indel) in the non-coding region and four single-nucleotide polymorphisms(SNPs) in the coding sequences of the Gm Tfl1 gene, four CAPS and one Indel markers were developed and used to test 1120 Chinese soybean varieties. We found that the dominant Gm Tfl1 allele was prevalent in accessions from the Northern ecoregion, whereas the recessive allele, Gmtfl1, was more common in the Southern ecoregion, and the proportions of Gm Tfl1 and recessive alleles were respectively 40.1% and 59.9% in the Huang-Huai ecoregion. The proportion of Gm Tfl1 decreased and that of Gmtfl1 increased, gradually from north to south. Allele Gm Tfl1-a was present in higher proportions in the Huang-Huai spring, Huang-Huai summer, and Northern spring sub-ecoregions than that in the other sub-ecoregions. Gm Tfl1-b was common in the Northeast spring, Northern spring and Southern summer sub-ecoregions. Gmtfl1-ta was found mainly in the Huang-Huai spring,Huang-Huai summer and Southern spring sub-ecoregions. The Gmtfl1-ab allele was distributed in all six soybean sub-ecoregions. The Gmtfl1-bb allele was distributed mainly in the Huang-Huai spring and summer and Southern spring and summer sub-ecoregions,but the Gmtfl1-tb allele was detected only in the Huang-Huai summer sub-ecoregion. The distributions of Gm Tfl1 and Gmtfl1 have shown no large changes in nearly 60 years of breeding, but the frequency of the recessive genotype Gmtfl1 has shown a rising trend in the last 20 years. This study provides a theoretical foundation for breeding new soybean varieties for different ecoregions. 关 键 词：SOYBEAN STEM GROWTH HABIT GM Tfl1 Distribution