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LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA

查看全文 作　　者：Mingwei [1]Chen;Xuedong [1]Wei;Xiu [2]Shi;Le [1]Lu;Guangbo [1,3]Zhang;Yuhua [1]Huang;Jianquan [1]Hou 高影响力作者 机构地区：[1]Department of Urology,The First Affiliated Hospital of Soochow University,Suzhou 215006,China;[2]Department of Obstetrics and Gynecology,The First Affiliated Hospital of Soochow University,Suzhou 215006,China;[3]Jiangsu Institute of Clinical Immunology,The First Affiliated Hospital of Soochow University,Jiangsu Key Laboratory of Clinical Immunology,Soochow University,Jiangsu Key Laboratory of Gastrointestinal Tumor Immunology,Suzhou 215006,China高影响力机构 出　　处：《Cancer Biology & Medicine》索引2021年第18卷第2期,共17页高影响力期刊 基　　金：supported by grants from the National Natural Science Foundations of China(Grant Nos.81702511,81472401,81772708,and 2016YFA0201204);the Jiangsu Provincial Key Medical Discipline(Grant No.ZDXKA2016012);the Clinical Medicine Center of Suzhou(Grant No.SZZXJ201501);programs for Recruitment of Clinical Medical Top Team of Suzhou。 摘　　要：Objective:Several reports have proposed that lnc RNAs,as potential biomarkers,participate in the progression and growth of malignant tumors.HIF1 A-AS2 is a novel lnc RNA and potential biomarker,involved in the genesis and development of carcinomas.However,the molecular mechanism of HIF1 A-AS2 in renal carcinoma is unclear.Methods:The relative expression levels of HIF1 A-AS2 and miR-30 a-5 p were detected using RT-qPCR in renal carcinoma tissues and cell lines.Using loss-of-function and overexpression,the biological effects of HIF1 A-AS2 and miR-30 a-5 p in kidney carcinoma progression were characterized.Dual luciferase reporter gene analysis and Western blot were used to detect the potential mechanism of HIF1 A-AS2 in renal carcinomas.Results:HIF1 A-AS2 was upregulated in kidney carcinoma tissues when compared with para-carcinoma tissues(P<0.05).In addition,tumor size,tumor node mestastasis stage and differentiation were identified as being closely associated with HIF1 A-AS2 expression(P<0.05).Knockdown or overexpression of HIF1 A-AS2 either restrained or promoted the malignant phenotype and WNT/β-catenin signaling in renal carcinoma cells(P<0.05).Mi R-30 a-5 p was downregulated in renal cancers and partially reversed HIF1 A-AS2 functions in malignant renal tumor cells.HIF1 A-AS2 acted as a micro RNA sponge that actively regulated the relative expression of SOX4 in sponging miR-30 a-5 p and subsequently increased the malignant phenotypes of renal carcinomas.HIF1 A-AS2 showed a carcinogenic effect and miR-30 a-5 p acted as an antagonist of the anti-oncogene effects in the pathogenesis of renal carcinomas.Conclusions:The HIF1 A-AS2-miR-30 a-5 p-SOX4 axis was associated with the malignant progression and development of renal carcinoma.The relative expression of HIF1 A-AS2 was negatively correlated with the expression of miR-30 a-5 p,and was closely correlated with SOX4 mRNA levels in renal cancers. 关 键 词：HIF1A-AS2 SOX4 miR-30a-5p kidney carcinoma ceRNA
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