The direct targets of CBFs:In cold stress response and beyond

查看全文 作　　者：Yue [1]Song;Xiaoyan [1]Zhang;Minze [1]Li;Hao [1]Yang;Diyi [1]Fu;Jian [1]Lv;Yanglin [1]Ding;Zhizhong [1]Gong;Yiting [1]Shi;Shuhua [1]Yang

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,China

出　　处：《Journal of Integrative Plant Biology》索引2021年第63卷第11期,共14页

基　　金：This work was supported by the National Natural Sciences Foundation of China(31872658,32022008,31921001)。 摘　　要：Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive(COR) genes. Essential to this process is the C-repeat binding factor(CBF)-dependent pathway, involving the activity of AP2/ERF(APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing(ChIP-seq) to determine the genomewide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat(CRT)/dehydrationresponsive elements(CRT/DRE;G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light,and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants. 关键词：ARABIDOPSIS CBF target genes CHIP-SEQ cold acclimation

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The direct targets of CBFs:In cold stress response and beyond

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