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| 1 | Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis显示文摘Molecular and genetic characterizations of mutants have led to a better understanding of many developmental processes in the model system Arabidopsis thaliana. However, the leaf development that is specific to plants has been little studied. With the aim of contributing to the genetic dissection of leaf development, we have performed a large-scare screening for mutants with abnormal leaves. Among a great number of leaf mutants we have generated by T-DNA and transposon tagging and ethylmethae sulfonate (EMS) mutagenesis, four independent mutant lines have been identified and studied genetically. Phenotypes of these mutant lines represent the defects of four novel nuclear genes designated LL1 (LOTUS LEAF 1), LL2 (LOTUS LEAF 2), URO (UPRIGHT ROSETTE), and EIL (ENVIRONT CONDITION INDUCED LESION). The phenotypic analysis indicates that these genes play important roles during leaf development. FOr the further genetic analysis of these genes and the map-based cloning of LL1 and LL2, we have mapped these genes to chromosome regions with an efficient and rapid mapping method. | SUN YUE WEI ZHANG FENG LING LI YING LI GUO TIAN LEI LIU HAI HUANG | 2000 | Cell Research2000，10，4： | 53 |
| 2 | Cold signaling in plants:Insights into mechanisms and regulation显示文摘To survive under cold temperatures plants must be able to perceive a cold signal and transduce it into downstream components that induce appropriate defense mechanisms. In addition to inducing adaptive defenses, such as the production of osmotic factors to prevent freezing and the reprogramming of transcriptional pathways, cold temperatures induce changes in plant growth and development which can affect the plant life cycle. In this review, we summarize recent progress in characterizing cold-related genes and the pathways that allow transduction of the cold signal in plants, focusing primarily on studies in Arabidopsis thaliana and rice(Oryza sativa). We summarize cold perception and signal transduction from the plasma membrane to the nucleus,which involves cold sensors, calcium signals, calciumbinding proteins, mitogen-activated protein kinase cascades, and the C-repeat binding factor/dehydrationresponsive element binding pathways, as well as trehalose metabolism. Finally, we describe the balance between plant organogenesis and cold tolerance mechanisms in rice. This review encapsulates the known cold signaling factors in plants and provides perspectives for ongoing cold signaling research. | Xiaoyu Guo Dongfeng Liu Kang Chong | 2018 | Journal of Integrative Plant Biology2018，60，9： | 42 |
| 3 | Genome-Wide Analysis of WOX Gene Family in Rice,Sorghum,Maize,Arabidopsis and Poplar显示文摘WUSCHEL-related homeobox (WOX) genes form a large gene family specifically expressed in plants.They are known to play important roles in regulating the development of plant tissues and organs by determining cell fate.Recent available whole genome sequences allow us to do more comprehensive phylogenetic analysis of the WOX genes in plants.In the present study,we identified 11 and 21 WOXs from sorghum (Sorghum bicolor) and maize (Zea mays),respectively.The 72 WOX genes from rice (Oryza sativa),sorghum,maize,Arabidopsis (Arabidopsis thaliana) and poplar (Populus trichocarpa) were grouped into three well supported clades with nine subgroups according to the amino acid sequences of their homodomains.Their phylogenetic relationship was also supported by the observation of the motifs outside the homodomain.We observed the variation of duplication events among the nine sub-groups between monocots and eudicots,for instance,more gene duplication events of WOXs within subgroup A for monocots,while,less for dicots in this subgroup.Furthermore,we observed the conserved intron/exon structural patterns of WOX genes in rice,sorghum and Arabidopsis.In addition,WUS (Wuschel)-box and EAR (the ERF-associated amphiphilic repression)-like motif were observed to be conserved among several WOX subgroups in these five plants.Comparative analysis of expression patterns of WOX genes in rice and Arabidopsis suggest that the WOX genes play conserved and various roles in plants.This work provides insights into the evolution of the WOX gene family and is useful for future research. | Xin Zhang Jie Zong Jianhua Liu Jinyuan Yin Dabing Zhang | 2010 | Journal of Integrative Plant Biology2010，52，11： | 38 |
| 4 | A Single-Cell RNA Sequencing Profiles the Developmental Landscape of Arabidopsis Root显示文摘Cells of eukaryotic multicellular organisms have inherent heterogeneity.Recent advances in single-cell gene expression studies enable us to explore transcriptional regulation in dynamic development processes and highly heterogeneous cell populations.In this study,using a high-throughput single-cell RNA-sequencing assay,we found that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes.A total of 24 putative cell clusters and the cluster-specific marker genes were identified.The spatial distribution and temporal ordering of the individual cells at different developmental stages illustrate their hierarchical structures and enable the reconstruction of continuous differentiation trajectory of root development.Moreover,we found that each root cell cluster exhibits distinct patterns of ion assimilation and hormonal responses.Collectively,our study reveals a high degree of heterogeneity of root cells and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution.We also established a web server(http://wanglab.sippe.ac.cn/rootatlas/)to facilitate the use of the datasets generated in this study. | Tian-Qi Zhang Zhou-Geng Xu Guan-Dong Shang Jia-Wei Wang | 2019 | Molecular Plant2019，12，5： | 37 |
| 5 | OsELF3 Is Involved in Circadian Clock Regulation for Promoting Flowering under Long-Day Conditions in Rice显示文摘标题日期是决定在米饭(Oryza sativa ) 收割季节和地区性的适应性的一个批评特点。研究努力在最后十年期间识别了在 Arabidopsis 和米饭之间被保存的一些重要光周期小径基因。在这研究,我们识别了新奇基因, Oryza sativa ELF3 (OsELF3 ) ,它是在 Arabidopsis thaliana 的 ELF3 基因的一个通常认为的相当或相同的事物。OsELF3 为在长天的条件下面领导日期的控制被要求。它的标注 Tos17 异种在仅仅下面展出了推迟的标题日期显型长天,然而并非短天,条件。OsELF3 高度在叶片被表示,并且 OsELF3 蛋白质在核是局部性的。一明显日报 OsELF3 抄本水平的节奏被观察,与在早白天内的马槽和在在野类型的植物的迟了的夜里的一座山峰。然而,这个表示模式在 oself3 异种被破坏。进一步的调查证明 OsGI 和 Ghd7 的表示在 oself3 异种是起来调整的,显示 OsELF3 充当在在长天的条件下面的花时间控制的 OsGI 和 Ghd7 在上游的一个否定管理者。包括一些 OsPRR 成员,生理节奏的钟相关的基因的有韵律的表示显然在 oself3 异种被影响。我们的结果显示 OsELF3 在米饭与生理节奏的钟经由它的串音在长天的 photoperiodic 小径充当花的使活跃之物。 | Ying Yang Qiang Peng Guo-Xing Chen Xiang-Hua Li Chang-Yin Wu | 2013 | Molecular Plant2013，6，1： | 24 |
| 6 | Photoactivated CRY1 and phyB Interact Directly with AUX/IAA Proteins to Inhibit Auxin Signaling in Arabidopsis显示文摘光是在 Arabidopsis 通过蓝色和调停 cryptochrome 的 red/far-red 光光敏电阻器和调停 phytochrome 的小径禁止胚轴房间延伸的关键环境暗示。相反,作为枢轴的内长的植物激素,植物生长素通过 AUX/IAA 蛋白质(AUX/IAAs ) 的植物生长素受体 TIR1/AFBs-mediated 降级支持胚轴延伸。然而,位于发信号的光和植物生长素的对抗相互作用下面的分子的机制仍然保持不清楚。这里,我们报导光禁止由 cryptochrome 的蓝、红的轻依赖者的相互作用通过 AUX/IAAs 的稳定发信号的植物生长素 1 (CRY1 ) 并且有 AUX/IAAs 的 phytochrome B 分别地。有 AUX/IAAs 的 CRY1 的蓝被触发光的相互作用与 AUX/IAAs 禁止 TIR1 的协会,导致这些蛋白质的导致植物生长素的降级的压抑。我们的结果显示光敏电阻器下游地直接作为一样与植物生长素受体分享 AUX/IAAs 表明部件。我们建议由光敏电阻器和植物生长素受体的 AUX/IAA 蛋白质稳定性的那条对抗规定允许植物平衡光和植物生长素信号优化他们的生长。 | Feng Xu Shengbo He Jingyi Zhang Zhilei Mao Wenxiu Wang Ting Li Jie Hua Shasha Du Pengbo Xu Ling Li Hongli Lian Hong-Quan Yang | 2018 | Molecular Plant2018，11，4： | 24 |
| 7 | Malate transported from chloroplast to mitochondrion triggers production of ROS and PCD in Arabidopsis thaliana显示文摘规划房间死亡(PCD ) 是一个基本生物过程。在马赛克死亡 1 的缺乏(MOD1 ) ,质体局部性的 enoyl-ACP reductase,导致反应的氧种类(ROS ) 和 PCD 的累积,它能被 mitochondrial 建筑群压制我变化,从叶绿体显示一个信号到线粒体。然而,这个信号尚待被阐明。在这研究,通过克隆并且分析一系列 mod1 suppressors,我们揭示调整 mitochondrial ROS 和扳机 PCD 的产生的一条全面细胞器通讯小径。我们证明在 PLASTIDIAL NAD 依赖的 MALATE 脱氢酶(plNAD-MDH ) 的变化, chloroplastic DICARBOXYLATE TRANSPORTER 1 (DiT1 ) 并且(mMDH1 ) MITOCHONDRIAL MALATE 脱氢酶 1 能各在 mod1 救 ROS 累积和 PCD 显型,经由 malate 梭从叶绿体表明直接通讯到线粒体。进一步的研究证明这些元素在不同光周期条件下面在氧化还原作用动态平衡和植物生长起关键作用。而且,我们表明 ROS 水平和 PCD 显著地在房间,它能是戏剧性地稀释了由的对待 malate 的 HeLa 被增加人的基因 MDH2 击倒, Arabidopsis mMDH1 的 ortholog。这些结果揭开在植物和动物系统的一条保存导致 malate 的 PCD 小径,革命化我们在细胞器之间的通讯的理解。 | Yannan Zhao Lilan Luo Jiesi Xu Peiyong Xin Hongyan Guo Jian Wu Lin Bai Guodong Wang Jinfang Chu Jianru Zuo Hong Yu Xun Huang Jiayang Li | 2018 | Cell Research2018，28，4： | 24 |
| 8 | AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants显示文摘AtbHLH29 of Arabidopsis, encoding a bHLH protein, reveals a high similarity to the tomato FER which is proposed as a transcriptional regulator involved in controlling the iron deficiency responses and the iron uptake in tomato. For identification of its biological functions, AtbHLH29 was introduced into the genome of the tomato FER mutant T3238fer mediated by Agrobacterium tumefaciencs. Transgenic plants were regenerated and the stable integration of AtbHLH29 into their genomes was confirmed by Southern hybridization. Molecular analysis demonstrated that expression of the exogenous AtbHLH29 of Arabidopsis in roots of the FER mutant T3238fer enabled to complement the defect functions of FER. The transgenic plants regained the ability to activate the whole iron deficiency responses and showed normal growth as the wild type under iron-limiting stress. Our transformation data demonstrate that AtbHLH29 is a functional ortholog of the tomato FER and can completely replace FER in controlling the effective iron acquisition in tomato. Except of iron, FER protein was directly or indirectly involved in manganese homeostasis due to that loss functions of FER in T3238fer resulted in strong reduction of Mn content in leaves and the defect function on Mn accumulation in leaves was complemented by expression of AtbHLH29 in the transgenic plants. Identification of the similar biological functions of FER and AtbHLH29, which isolated from two systematically wide-diverged “strategy I” plants, suggests that FER might be a universal gene presented in all strategy I plants in controlling effective iron acquisition system in roots. | You Xi YUAN Juan ZHANG Dao Wen WANG Hong Qing LING | 2005 | Cell Research2005，15，8： | 22 |
| 9 | New insights into gibberellin signaling in regulating flowering in Arabidopsis显示文摘In angiosperms,floral transition is a key developmental transition from the vegetative to reproductive growth,and requires precise regulation to maximize the reproductive success.A complex regulatory network governs this transition through integrating flowering pathways in response to multiple exogenous and endogenous cues.Phytohormones are essential for proper plant developmental regulation and have been extensively studied for their involvement in the floral transition.Among various phytohormones,gibberellin(GA)plays a major role in affecting flowering in the model plant Arabidopsis thaliana.The GA pathway interact with other flowering genetic pathways and phytohormone signaling pathways through either DELLA proteins or mediating GA homeostasis.In this review,we summarize the recent advances in understanding the mechanisms of DELLA-mediated GA pathway in flowering time control in Arabidopsis,and discuss its possible link with other phytohormone pathways during the floral transition. | Shengjie Bao Changmei Hua Lisha Shen Hao Yu | 2020 | Journal of Integrative Plant Biology2020，62，1： | 21 |
| 10 | EL1-like Casein Kinases Suppress ABA Signaling and Responses by Phosphorylating and Destabilizing the ABA Receptors PYR/PYLs in Arabidopsis显示文摘揭开植物激素 abscisic 酸(骆驼毛的织物) 和它的规章的机制的信号 transduction 为向改进植物回答到紧张的环境开发策略是批评的。骆驼毛的织物发信号被多重 PYR/PYL 受体察觉并且调停,谁的 translational 以后修正,特别 phosphorylation,大部分未知的遗体。在这研究,我们示威 ? 那 Arabidopsis 象 EL1 一样(AEL ) 蛋白质,调整各种各样的生理的过程的酷蛋白 kinase,支持他们的 ubiquitination 和降级的 phosphorylate PYR/PYLs,导致压制的骆驼毛的织物回答。Arabidopsis ael 三元组异种显示过分敏感的回答到骆驼毛的织物处理,它与 PYR/PYL 蛋白质的压制的降级一致。PYR/PYLs 是 phosphorylated 在 ? 保存 AEL phosphorylation 地点的 vivo 和变化导致 PYR/PYLs,和因此提高的骆驼毛的织物回答的减少的 phosphorylation, ubiquitination,和降级。一起拿,这些结果证明调停 AEL 的 phosphorylation 在调整 PYR/PYLs 的稳定性和功能起关键作用,提供重要卓见进 PYR/PYL 受体并且骆驼毛的织物发信号的 translational 以后规定。 | Hu-Hui Chen Li Qu Zhi-Hong Xu Jian-Kang Zhu Hong-Wei Xue | 2018 | Molecular Plant2018，11，5： | 20 |
| 11 | 5-Methylcytosine RNA Methylation in Arabidopsis Thaliana显示文摘5-Methylcytosine (m 5 C ) 是描绘得好的 DNA 修正,并且主要也在初核质和优核质在丰富的非编码的 RNA 被报导。然而,分发和 m 5 在植物 mRNAs 的 C 仍然保持大部分未知。这里,我们报导 RNA m 5 在由使用 m 5 C RNA immunoprecipitation 由一条深定序的途径列在后面(m 5 C-RIP-seq ) 。LC-MS/MS 和点污点分析揭示 m 5 在各种各样的纸巾并且在不同发展阶段的 C mRNA 修正。m 5 C-RIP-seq 分析识别了 6045 m 5 在 4465 的 C 山峰在年轻幼苗表示了基因。我们发现那 m 5 C 在编码序列,二座山峰在开始 codons 以后并且在站 codons 前立即定位了被充实,并且随低翻译与 mRNAs 被联系活动。我们进一步表明了那 RNA (cytosine-5 )-methyltransferase,tRNA 特定的 methyltransferase 4B (TRM4B ) ,展览 m 5 C RNA methyltransferase 活动。在 TRM4B 的变化在根开发和减少的 m 5 C 山峰。TRM4B 影响涉及根开发的基因的抄本层次,它断然与他们的 mRNA 稳定性和 m 5 C 层次。我们的结果建议那 m 5 在 mRNA 的 C 是新 epitranscriptome 标记 inArabidopsis,并且这修正的那条规定是基因的不可分的部分规章的网络位于 \O 下面植物开发。 | Xuean Cui Zhe Liang Lisha Shen Qian Zhang Shengjie Bao Yuke Geng Bin Zhang Vonny Leo Leah A. Vardy Tiegang Lu Xiaofeng Gu Hao Yu | 2017 | Molecular Plant2017，10，11： | 19 |
| 12 | A Phytophthora capsici RXLR Effector Targets and Inhibits a Plant,PPlase to Suppress Endoplasmic Reticulum-Mediated Immunity显示文摘 | Guangjin Fan Yang Yang Tingting Li Wenqin Lu Yu DU Xiaoyu Qiang Qujiang Wen Weixing Shan | 2018 | Molecular Plant2018，11，8： | 18 |
| 13 | Four IVa bHLH Transcription Factors Are Novel Interactors of FIT and Mediate JA Inhibition of Iron Uptake in Arabidopsis显示文摘工厂发展了复杂基因网络调整铁(Fe ) 为他们的幸存的动态平衡。包括 jasmonic 酸(JA ) 的工厂荷尔蒙的几个类被显示了涉及在工厂调整铁举起或缺乏应答的基因的表达式。然而, JA 由调整铁举起的分子的机制仍然保持不清楚。在这研究,我们发现 JA 否定地由 downregulating 调制铁举起表示合适(bHLH29 ) , bHLH38, bHLH39, bHLH100,和 bHLH101 并且支持合适的蛋白质的降级,在 Arabidopsis 的铁举起的一个关键管理者。我们进一步证明亚群 IVa bHLH 蛋白质, bHLH18, bHLH19, bHLH20,和 bHLH25,是新奇 interactors 适合,它支持导致 JA 的合适的蛋白质降级。这四 IVa bHLHs 冗余地工作反对 Ib bHLHs 的活动(例如 bHLH38 ) 在在铁下面调整合适的蛋白质稳定性缺乏。四 IVa bHLH 基因首先在根被表示,并且由 JA 处理是可诱导的。而且,我们发现了那 MYC2 和 JAR1 ,表明小径的 JA 的二个批评部件,在调停起关键作用表示的 JA 抑制合适并且 Ib bHLH 基因他们差别调制 bHLH18 的表示, bHLH19 , bHLH20 ,并且调整的 bHLH25 在铁缺乏下面适合累积。一起拿,这些结果显示由 transcriptionally 调整 bHLH 基因的不同集合的 ?表示,发信号的 JA 支持合适的降级,导致铁举起的减少的表示基因, IRT1 和 FRO2,和到铁的增加的敏感缺乏。我们的数据 ? 建议当面有铁缺乏反应的 multilayered 抑制在 Arabidopsis 的 JA。 | Yan Cui Chun-Lin Chen Man Cui Wen-Juan Zhou Hui-Lan Wu Hong-Qing Ling | 2018 | Molecular Plant2018，11，9： | 16 |
| 14 | The Antagonistic Action of Abscisic Acid and Cytokinin Signaling Mediates Drought Stress Response in Arabidopsis显示文摘 | Xiaozhen Huang Lingyan Hou Jingjing Meng Huiwen You Zhen Li Zhizhong Gong Shuhua Yang Yiting Shi | 2018 | Molecular Plant2018，11，7： | 15 |
| 15 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
| 16 | Turning rice meiosis into mitosis显示文摘通过在庄稼的种子(单性生殖) 的同种细胞的复制的介绍有潜力由允许任何精英变化的自我繁殖革命化农业,特别地 F1 混血儿。在性模型植物 Arabidopsis thaliana,通过种子的合成同种细胞的复制能被联合三个变化把成熟分裂变成有丝分裂(笑剧) 并且(的(i) 人工地实现 ii ) 穿过获得的同种细胞的配偶子,一根线表示修改了 CENH3 并且其染色体在接合子被消除。这里,我们证明变化的另外的联合能把 Arabidopsis 成熟分裂变成有丝分裂并且在米饭(Oryza sativa ) 的三个变化的联合高效地把成熟分裂变成有丝分裂,导致在这主要庄稼的男、女的同种细胞的双配偶子的生产。在种系发生地远的 eudicot Arabidopsis 和 monocot 米饭的笑剧技术的成功的实现为它的申请打开门到任何 flowering 植物并且为在庄稼种类介绍单性生殖铺平道路。 | Delphine Mieulet Sylvie Jolivet Maud Rivard Laurence Cromer Aurore Vemet Pauline Mayonove Lucie Pereira Gaetan Droc Brigitte Courtois Emmanuel Guiderdoni Raphael Mercier | 2016 | Cell Research2016，26，11： | 14 |
| 17 | An Arabidopsis ABC Transporter Mediates Phosphate Deficiency-Induced Remodeling of Root Architecture by Modulating Iron Homeostasis in Roots显示文摘改变根建筑学是到磷酸盐(Pi ) 的植物的主要发展回答缺乏并且被认为在表层土为可得到的 Pi 提高一个植物能力到牧草。然而,控制这回答的内在的机制糟糕被理解。在这研究,我们识别了 Arabidopsis 异种, hps10 (对 Pi 饥饿 10 过分敏感) ,它在 Pi 足够的状况下面是词法上正常的,但是在 Pi 下面显示出主要的根生长和侧面的根的提高的生产的增加的抑制缺乏。hps10 是 ALUMINUM SENSITIVE3 (ALS3 ) 基因的以前识别的等位基因(als3-3 ) ,它涉及植物忍耐到铝毒性。我们的结果证明 ALS3 和它的交往的蛋白质 AtSTAR1 在 tonoplast 形成 ABC transporter 建筑群。这蛋白质建筑群调停一高度在 Xenopus 卵母细胞的 electrogenic 运输。在 Pi 缺乏下面, als3 比野类型在它的根积累 Fe 3+ 的高水平。在 Arabidopsis, LPR1 (低磷酸盐 ROOT1 ) 和 LPR2 编码 ferroxidases,当变异时,它在根和原因根生长减少 Fe 3+ 累积对 Pi 缺乏感觉迟钝。这里,我们提供证明 ALS3 与 LPR1/2 合作调整 Pi 的引人注目的证据在根的由 modulating Fe 动态平衡的根建筑学的导致缺乏的改变。 | Jinsong Dong Miguel A. PiSeros Xiaoxuan Li Haibing Yang Yu Liu Angus S. Murphy Leon V. Kochian Dong Liu | 2017 | Molecular Plant2017，10，2： | 13 |
| 18 | Ligand-triggered de-repression of Arabidopsis heterotrimeric G proteins coupled to immune receptor kinases显示文摘 | Xiangxiu Liang Miaomiao Ma ,Zhaoyang Zhou Jinlong Wang Xinru Yang Shaofei Rao Guozhi Bi Lin Li Xiaojuan Zhang Jijie Chai She Chen Jian-Min Zhou | 2018 | Cell Research2018，28，5： | 13 |
| 19 | Marking RNA:m^6A writers,readers,and functions in Arabidopsis显示文摘N^6-methyladenosine(m^6A)emerges as an important modification in eukaryotic mRNAs.m^6A has first been reported in 1974,and its functional significance in mammalian gene regulation and importance for proper development have been well established.An arsenal of writer,eraser,and reader proteins accomplish deposition,removal,and interpretation of the m^6A mark,resulting in dynamic function.This led to the concept of an epitranscriptome,the compendium of RNA species with chemical modification ofthe nucleobases in the cell,in analogy to the epigenome.While m^6A has long been known to also exist in plant mRNAs,proteins involved in m^6A metabolism have only recently been detected by mutant analysis,homology search,and mRNA interactome capture in the reference plant Arabidopsis thaliana.Dysregulation ofthe m^6A modification causes severe developmental abnormalities of leaves and roots and altered timing of reproductive development.Furthermore,m^6A modification affects viral infection.Here,we discuss recent progress in identifying m^6A sites transcriptome-wide,in identifying the molecular players involved in writing,removing,and reading the mark,and in assigning functions to this RNA modification in 4.thaliana.We highlight similarities and differences to m^6A modification in mammals and provide an outlook on important questions that remain to be addressed. | Marlene Reichel Tino Koster Dorothee Staiger | 2019 | Journal of Molecular Cell Biology2019，11，10： | 13 |
| 20 | Regulation of Seed Vigor by Manipulation of Raffinose Family Oligosaccharides in Maize and Arabidopsis thaliana显示文摘棉白糖家庭 oligosaccharides (RFO ) 在许多植物种类在成熟干燥期间在种子积累。然而, RFO 是否在建立种子活力有一个角色,仍然保持不清楚。GALACTINOL SYNTHASE (GOLS ) ,棉白糖 SYNTHASE (RS ) ,和 STACHYOSE SYNTHASE (圣) 是为 RFO 负责的酶 ? 在植物的生合成。有趣地,仅仅棉白糖在玉米种子被检测,并且唯一的玉米 RS 基因(ZmRS ) 被识别。在这研究,我们发现二独立 mutator (亩) 打断了 zmrs 线,不包含棉白糖但是 hyperaccumulating galactinol, ? 显著地减少了种子活力,与空 segregant 控制相比。不同于玉米, Arabidopsis thaliana 种子包含几 RFO (棉白糖, stachyose,和 verbascose ) 。A 的操作。thaliana RFO ? 由 overexpressing ZmGOLS2, ZmRS,或 AtSTS 的内容表明了那 co-overexpression ZmGOLS2 和 ZmRS,或 ZmGOLS2 的 overexpression 独自一个,显著地增加了 RFO 的全部的内容并且提高了 Arabidopsis 种子活力。令人惊讶地,当 ZmRS 的 overexpression 增加了种子棉白糖内容时,它的 overexpression 戏剧性地减少了种子活力并且减少了 galactinol, stachyose,和 verbascose 的种子数量。相反, atrs5 异种种子类似于关于种子活力和 RFO 内容的野类型的那些,除了 stachyose 它在 atrs5 种子积累了。全部的 RFO, RFO/sucrose 比率,然而并非绝对单个 RFO ? 数量,断然与 A 相关。thaliana 种子活力, stachyose 和 verbascose 多于棉白糖作出贡献到。一起拿,这些结果提供新卓见进种子活力的规章的机制并且在 monocot 和 dicot 在 modulating 种子活力为 RFO 揭示不同要求。 | Tao Li Yumin Zhang Dong Wang Ying Liu Lynnette M.A. Dirk Jack Goodman A. Bruce Downie Jianmin Wang Guoying Wang Tianyong Zhao | 2017 | Molecular Plant2017，10，12： | 13 |
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