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Generation of purple-violet chrysanthemums via anthocyanin B-ring hydroxylation and glucosylation introduced from Osteospermum hybrid F3'5'H and Clitoria ternatea A3'5'GT

查看全文 作　　者：Xiaoying [1]Han;Yuting [1]Luo;Jiaoyang [1]Lin;Huiying [1]Wu;Hao [2]Sun;Lijie [1]Zhou;Sumei [1]Chen;Zhiyong [1]Guan;Weimin [1]Fang;Fei [1]Zhang;Fadi [1]Chen;Jiafu [1]Jiang 高影响力作者 机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,Key Laboratory of Biology of Ornamental Plants in East China,National Forestry and Grassland Administration,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;[2]Department of Chemistry,College of Sciences,Nanjing Agricultural University,Nanjing 210095,China高影响力机构 出　　处：《Ornamental Plant Research》索引2021年第1卷第1期,共9页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(31930100,31902053);the earmarked fund for Jiangsu Agricultural Industry Technology System(JATS[2020]402);a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. 摘　　要：Chrysanthemums possess no metabolic pathway to synthesize delphinidin because of the lack of endogenous F3'5'H gene encoding the key enzyme in its biosynthetic pathway;therefore,there are no blue or blue-purple chrysanthemums occurring naturally.Currently,the introduction of exogenous F3'5'H into chrysanthemums is an efficient method for breeding bluish chrysanthemums.In this study,we explored the effects of the introduction of mutant CmF3'H(generated via site-directed mutagenesis,T485S,CmF3'Hm)and exogenous Osteospermum hybrid F3'5'H(OhF3'5'H)genes combined with Clitoria ternatea A3'5'GT(CtA3'5'GT)on delphinidin synthesis in chrysanthemum.Among the F3'5'H transgenic lines,those overexpressing endogenous CmF3'Hm could not generate blue flower color,although red color was changed to light pink due to CtA3'5'GT function.Meanwhile,OhF3'5'H introduction promoted the accumulation of delphinidin and its derivatives in chrysanthemum,changing the flower color from red-purple to purple-violet.These results indicate the applicability of exogenous OhF3'5'H and CtA3'5'GT transformation for promoting delphinidin synthesis during the molecular breeding of violet/blue chrysanthemums. 关 键 词：function VIOLET synthesis
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